The 14-3-3 Gene Function of Cryptococcus neoformans Is Required for its Growth and Virulence

( Jingbo Li ),( Yun C. Chang ),( Chun Hua Wu ),( Jennifer Liu ),( Kyung J. Kwon Chung ),( Sheng He Huang ),( Hiro Shimada ),( Rob Fante ),( Xiaowei Fu ),( Ambrose Jong ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.5

Cryptococcus neoformans is a life-threatening pathogenic yeast that causes devastating meningoencephalitis. The mechanism of cryptococcal brain invasion is largely unknown, and recent studies suggest that its extracellular microvesicles may be involved in the invasion process. The 14-3-3 protein is abundant in the extracellular microvesicles of C. neoformans, and the 14-3-3-GFP fusion has been used as the microvesicle’s marker. However, the physiological role of 14-3-3 has not been explored. In this report, we have found that C. neoformans contains a single 14-3-3 gene that apparently is an essential gene. To explore the functions of 14-3-3, we substituted the promoter region of the 14-3-3 with the copper-controllable promoter CTR4. The CTR4 regulatory strain showed an enlarged cell size, drastic changes in morphology, and a decrease in the thickness of the capsule under copper-enriched conditions. Furthermore, the mutant cells produced a lower amount of total proteins in their extracellular microvesicles and reduced adhesion to human brain microvascular endothelial cells in vitro. Proteomic analyses of the protein components under 14-3-3-overexpressed and -suppressed conditions revealed that the 14-3-3 function(s) might be associated with the microvesicle biogenesis. Our results support that 14-3-3 has diverse pertinent roles in both physiology and pathogenesis in C. neoformans. Its gene functions are closely relevant to the pathogenesis of this fungus.

Preparation of Nano-Convex and Nano-Concave-Patterned Polyimide Surfaces and their Nano-Tribological Behavior

Chunxia Wu,XIAOLIANG ZHANG,HONGWEI CHE,JINGBO MU,Guangshuo Wang,Zhixiao Zhang,Guohua Cui 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.4

In this work, nano-convex-patterned polyimide surface (notated as 1-sample) and nano-concavepatterned polyimide surface (notated as 2-sample) were prepared by self-assembly and etching. Atomic force microscope (AFM) with a colloidal probe was used to examine the adhesion and nano-tribological behavior of the 1-sample and 2-sample. Results suggest that the 1-sample and 2-sample can decrease the surface friction and adhesive forces because of the decreased contact area between the contacting pairs. The friction forces of the 1-sample and 2-sample increased with the increase in sliding velocity and applied load. Moreover, the nano-concave pattern is more effective in reducing the adhesive force than the nano-convex pattern because of its higher surface roughness. However, the nano-convex patterning is more effective in reducing the friction force than the nano-concave patterning because of the smaller area of contact between the 2-sample and the colloidal probe.

ECLOGITES AND THEIR COUNTRY GNEISSES STUDIES ON INCLUSIONS IN THE ACCESSORY MINERALS OF COUNTRY GNEISSES

LIU JINGBO,WU YIN,GUO LIANJIE 대한지질학회 1997 대한지질학회 학술대회 Vol.1997 No.

Response evaluation and vibration control of a transmission tower-line system in mountain areas subjected to cable rupture

Chen, Bo,Wu, Jingbo,Ouyang, Yiqin,Yang, Deng Techno-Press 2018 Structural monitoring and maintenance Vol.5 No.1

Transmission tower-line systems are commonly slender and generally possess a small stiffness and low structural damping. They are prone to impulsive excitations induced by cable rupture and may experience strong vibration. Excessive deformation and vibration of a transmission tower-line system subjected to cable rupture may induce a local destruction and even failure event. A little work has yet been carried out to evaluate the performance of transmission tower-line systems in mountain areas subjected to cable rupture. In addition, the control for cable rupture induced vibration of a transmission tower-line system has not been systematically conducted. In this regard, the dynamic response analysis of a transmission tower-line system in mountain areas subjected to cable rupture is conducted. Furthermore, the feasibility of using viscous fluid dampers to suppress the cable rupture-induced vibration is also investigated. The three dimensional (3D) finite element (FE) model of a transmission tower-line system is first established and the mathematical model of a mountain is developed to describe the equivalent scale and configuration of a mountain. The model of a tower-line-mountain system is developed by taking a real transmission tower-line system constructed in China as an example. The mechanical model for the dynamic interaction between the ground and transmission lines is proposed and the mechanical model of a viscous fluid damper is also presented. The equations of motion of the transmission tower-line system subjected to cable rupture without/with viscous fluid dampers are established. The field measurement is carried out to verify the analytical FE model and determine the damping ratios of the example transmission tower-line system. The dynamic analysis of the tower-line system is carried out to investigate structural performance under cable rupture and the validity of the proposed control approach based on viscous fluid dampers is examined. The made observations demonstrate that cable rupture may induce strong structural vibration and the implementation of viscous fluid dampers with optimal parameters can effectively suppress structural responses.

Hypolipidemic Effects of Hickory Nut Oil using Cold Pressure Extraction

Yan Zhang,Liying Wu,Zhixin Yao,Zhongsu Ma,Jingbo Liu 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.suppl1

Optimal conditions of hickory nut oil cold press technology were studied. L9(34) orthogonal experiment results showed that optimal conditions were a pressing pressure of 15 MPa, pressing temperature of 50oC, pressing cycle of 4 s, and stop cycle of 9 s. Fatty acid compositions were determined using GC-MS and hypolipidemic effects in mice were investigated. Compared to a high fat diet group, hickory nut oil administration decreased serum and visceral total cholesterol, triacylglycerol, and serum low density lipoprotein cholesterol levels. Serum high density lipoprotein cholesterol values were increased. Hickory nut oil can be used as a valuable bioactive source of natural hypolipidemic compounds.

Research and Practice of Full Plugging Groundwater Technology in Water-Rich TBM Diversion Tunnel

Lichuan Wang,Shuai Zhang,Zongqing Zhou,Jian Wu,Tao Bai,Yunjuan Chen,Jingbo Zhao,Shuo Xu 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.2

To solve the problem of water discharge in the tunnel when the tunnel passes through the low-pressure water-rich fractured stratum, the grouting plugging technology of the low-pressure water-rich tunnel is studied. The grouting plugging scheme is optimized through the field test. The post-validation research is numerically simulated and the parameters of the complete sealing grouting ring are analyzed. The results show that: 1) Full plugging grouting can effectively improve the funnel effect of precipitation with good water plugging effect. With the decrease of the permeability coefficient and the increase of the thickness of the grouting ring, the flow velocity around the tunnel and the water discharge in the tunnel both decreased significantly, with a trend of “rapidly decreasing-slowly decreasing”. Considering various factors, an optimal grouting ring thickness between 1.5 m and 2.5 m and a reasonable range for permeability coefficient between 2.07 × 10−6 cm/s and 4.14 × 10−6 cm/s are considered appropriate. 2) The use of cement-water glass system full plugging grouting for water plugging, can significantly reduce the discharge of water, only partial dripping or even dripping state. Using polyurethane foam single point method to systematically block grouting and water plugging can only changes the discharge path and hardly attenuates water discharge.

Synthesis of aminated calcium lignosulfonate and its adsorption properties for azo dyes

Yingying Wang,Linli Zhu,Xiaohong Wang,Wanru Zheng,Chen Hao,Chenglong Jiang,Jingbo Wu 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.61 No.-

A low-cost adsorbent, aminated calcium lignosulfonate (ACLS) was prepared and successfully applied to the adsorption of Congo red and the Titan yellow dyes. The adsorbent was characterized by scanning electron microscopy (SEM), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), elemental mapping images (EMIs) and Brunauer–Emmett–Teller (BET) analysis. And the efficiency of ACLS for the removal of Congo red and the Titan yellow dyes was evaluated by several factors, such as temperature, pH, adsorbent dose, contact time and initial concentration of dyes solution. And the test ranges of temperature, pH, adsorbent dose, contact time and initial concentration of dye solution were 25–45 °C, 2–12, 0.005–0.05 g, 1–48 h, 10–200 mg L−1, respectively. The adsorption results demonstrated a good ability to remove dye with the removal rates of 97% and 91% for 30 mg L−1 Congo red and 40 mg L−1 Titan yellow, respectively. The adsorption kinetic and adsorption isotherms can be well described by the pseudo second order kinetic and the Langmuir isotherm model for the both dyes, respectively. Moreover, the maximum adsorption capacity of Congo red and Titan yellow reached 258.4 mg g−1 and 190.1 mg g−1 in the study of the Langmuir adsorption isotherm, respectively. Thermodynamic studies show that the adsorption of the two dyes is a spontaneous endothermic process. The results indicate that the ACLS has the potential to be used in the treatment of dye wastewater.

Up-regulation of Insulin-like Growth Factor Binding Protein-3 Is Associated with Brain Metastasis in Lung Adenocarcinoma

Yang, Lishi,Li, Junyang,Fu, Shaozhi,Ren, Peirong,Tang, Juan,Wang, Na,Shi, Xiangxiang,Wu, Jingbo,Lin, Sheng Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.4

The brain is the most common metastatic site of lung adenocarcinoma; however, the mechanism of this selective metastasis remains unclear. We aimed to verify the hypothesis that exposure of tumor cells to the brain microenvironment leads to changes in their gene expression, which promotes their oriented transfer to the brain. A549 and H1299 lung adenocarcinoma cells were exposed to human astrocyte-conditioned medium to simulate the brain microenvironment. Microarray analysis was used to identify differentially expressed genes, which were confirmed by quantitative real-time PCR and western blotting. Knockdown experiments using microRNAs and the overexpression of genes by cell transfection were performed in addition to migration and invasion assays. In vitro findings were confirmed in clinical specimens using immunohistochemistry. We found and confirmed a significant increase in insulin-like growth factor binding protein-3 (IGFBP3) levels. Our results also showed that the up-regulation of IGFBP3 promoted A549 cell epithelial-mesenchymal transition, migration, and invasion, while the knockdown of IGFBP3 resulted in decreased cell motility. We also found that Transforming growth factor-${\beta}$ (TGF-${\beta}$)/Mothers against decapentaplegic homolog 4 (Smad4)-induced epithelial-mesenchymal transition was likely IGFBP3-dependent in A549 cells. Finally, expression of IGFBP3 was significantly elevated in pulmonary cancer tissues and intracranial metastatic tissues. Our data indicate that up-regulation of IGFBP3 might mediate brain metastasis in lung adenocarcinoma, which makes it a potential therapeutic target.