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      • KCI등재

        Artificial diet development for mass rearing and its effect on the reproduction of yellow peach moth, Conogethes punctiferalis (Guenée)

        Jing Dapeng,Zhang Tiantao,Bai Shuxiong,He Kanglai,Prabu Sivaprasath,Wang Zhenying 한국곤충학회 2021 Entomological Research Vol.51 No.3

        Yellow peach moth, Conogethes punctiferalis(Guenée) (Lepidoptera: Crambidae), is a very destructive fruit borer in Eastern Asia and Australia. A successful artificial diet and rearing protocol are urgently needed for its biological control and research. In this study, we developed and assessed four artificial diets: AD-1, AD-2, AD-3 and AD-4, for rearing yellow peach moth larvae. AD-1 is effective supplements, leading to the larval survival rate of 82.05%, percentage of pupation 79.48%, mean pupa weight of 76.53 mg, emergence rate of 95.25%, and an adult fecundity rate of 121 eggs/female. Using it to feed can shorten the whole generation developmental period to 33 days. Based on this diet AD-1, we also develop a suitable mass rearing method. During the larval stages providing three- to four-time fresh diet can keep a healthy larval colony.

      • KCI등재

        Genetic and antigenic characterization of fragments of the pheasant immunoglobulin Y heavy chain constant region

        Jing Zhai,Weishan Chang,Zhenzhen Zhai,Xingliang Ma,Lingling Yang,Jinfeng Ti,Daozhen Song,Shijun Fu,Dapeng Li 한국유전학회 2016 Genes & Genomics Vol.38 No.8

        Few studies have attempted to characterize the pheasant (Phasianus colchicus) immunoglobulin Y (IgY) heavy chain constant region. In the present study, fragments of the pheasant IgY heavy chain constant region were cloned, analyzed, and expressed. The cross-reactivity of IgY or immunoglobulin G (IgG)s with antigens from other vertebrate species was determined using dot-enzymelinked immunosorbent assay and western blot analysis. Five peptides of the pheasant IgY heavy chain constant region were synthesized to determine its immunoregulatory activity in vitro. The IgY heavy chain constant region from pheasant showed the highest homology with that from chicken (71.2 %) and duck (49.1 %). Phylogenetic analysis for IgY showed that pheasant was closely related to chicken and duck than to any other analyzed vertebrate species. The rabbit anti-chicken IgG showed immunologic cross-reactivity with recombinant proteins of the pheasant IgY heavy chain constant region. Four peptides were able to induce significant up-regulation of interleukin (IL)-1b, IL-4, and interferon-c in chicken peripheral blood lymphocytes, suggesting a new role of avian IgY in immune regulation.

      • KCI등재

        Simultaneous activation of KHSO5 and BuOOH by iron octacarboxyphthalocyanine loaded on fly ash microspheres to boost pollutant degradation

        Dapeng Li,Peng Zhang,Jiangtao Duan,Yaxin Wu,Na Ding,Zhenyu Wan,Longqi Chen,Jingli Xu,Suxiang Ge,Juntao Ma 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

        Utilization of fly ash (FA) wastes as the carriers of catalysts for the environmental application is an economicand practical strategy for their low cost, easy accessibility and thermal stability. However, mostcatalysts immobilized on fly ash were metal or metal oxides, some potential catalysts such as metalphthalocyanine complexes have not been reported upon their heterogeneous catalysis with FA as the carriers. In this paper, highly active iron octacarboxyphthalocyanine were immobilized onto the bird nestlikesurfaces of fly ash microspheres after NaOH activation. It is noted that the FeOCPc@FA compositeswith only 2 wt.% exhibited the high catalytic efficiency in the organic dye degradation. The high concentrationof 30 mg/L rhodamine B and methylene blue could be fast decolorized in the presence ofFeOCPc@FA–2 % and KHSO5. Moreover, the ultrafast decolorization of these dyes could be observed inthe catalytic system composed of FeOCPc@FA–2 %, KHSO5 and BuOOH (tert-butyl hydroperoxide). Simultaneous activation of KHSO5 and BuOOH could be realized in our designed catalytic system. Based on the structural characterizations of composites and active species generated during the catalyticprocesses, the probable generation pathway of metal–oxygen active species and various radicals wereanalyzed to explicate the catalytic mechanism. Our investigation provides a high efficiency, low costand environmentally friendly strategy for advanced oxidation treatment of organic pollutants.

      • KCI등재

        Insecticidal activity of Origanum majorana L. essential oil as anti-cholinergic agent

        Prabu Sivaprasath,Jing Dapeng,Chandran Viswanathan,Mathew Preethy 한국곤충학회 2020 Entomological Research Vol.50 No.8

        The present study was focused on exploring the presence of active compounds in Origanum majorana essential oil (OmEO), and its various knock-down effects against the rice moth, Corcyra cephalonica. GC–MS analysis detected the existence of major compounds such as monoterpenes, cis-β-terpineol and terpinen-4-ol with the total proportion of 52.16%. Fumigant toxicity against adult and larvae was calculated with an LC50 value of 11.31 and 49.83 μL/L air, respectively. The contact toxicity against adult, pupa, larvae and eggs was observed with LC50 value 2.54, 0.95, 2.78, and 0.49 μL/L, respectively. Furthermore, the influential repellent behavior against adults has been observed. Acetylesterase (AChE) inhibition activity of OmEO was observed against adult and larvae of C. cephalonica with an IC50 value of 35.89 and 118.54 μL/mL, respectively. Moreover, computational docking study revealed the binding affinity of Cis-β-terpineol and terpinen-4-ol towards the active binding sites of AChE. On the other hand, Fluorescence-assisted cytometry and comet assay confirmed the cytotoxic and genotoxic effect of OmEO at various concentrations on C. cephalonica. Altogether, the results showed the knock-down effect of OmEO against C. cephalonica, and it could be a potential biocontrol measure against the stored product pest.

      • KCI등재

        Molecular identification and functional analysis of Niemann-Pick type C2 protein in Macrocentrus cingulum Brischke (Hymenoptera: Braconidae)

        Lin Maung Kyaw,Jing Dapeng,Zhang Tiantao,Prabu Sivaprasath,He Kanglai,Bai Shuxiong,Wang Zhenying 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

        Niemann-Pick type C2 (NPC2) proteins in arthropods have been extensively differentiated and possibly dupli cated according to environmental conditions and are probable to have different functions. The participation of NPC2 proteins in chemical communication in arthropods brings new objectives in environmental-friendly stra tegies for pest population control. In this study, NPC2 gene in Macrocentrus cingulum (McinNPC2) was newly identified by rapid amplification cDNA ends (RACE) technology. McinNPC2 amino acid sequence alignment with other representative NPC2 annotates to evaluate the highly conserved consensus amino acids, but with odorant binding proteins in M. cingulum show that only one consensus amino acid. Primary six-cysteine structures that are same to odorant binding proteins in M. cingulum were observed in McinNPC2. Phylogenetic analysis of McinNPC2 indicated that the nearest monophyletic group forming one clade with high posterior probability values clusters as Cyphomyrmex costatus (CcosNPC2) whereas the nearest evolutionary relation group as some odorant binding proteins. Moreover, quantitative real-time PCR (qPCR) measurements show that the McinNPC2 gene expression level in various tissues of the female is significantly and ubiquitously higher than in male, whereas the highest expression level in female antennae. We further explore the binding characterization of recombinant McinNPC2 to candidate odor molecules and did the modeling and docking simulations. The results showed ligands binding specificity and docking tests results indicate that β-ionone, an aroma compound commonly found in essential oils, can strongly bind with McinNPC2. In conclusion, we proposed that McinNPC2 may be involved in chemical communication and play roles in perception of plant volatiles.

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