Effects of Free Anthraquinones Extract from the Rhubarb on Cell Proliferation and Accumulation of Extracellular Matrix in High Glucose Cultured-Mesangial Cells

Jianyun Wang,Hui Fang,Bingzheng Dong,Dongdong Wang,Yan Li,Xiao Chen,Lijuan Chen,Tong Wei,Qunli Wei 대한약리학회 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6

Diabetic nephropathy (DN) is the leading cause of end-stage failure of the kidney, but the efficacy of currently available strategies for the prevention of DN remains unsatisfactory. In this study, we investigated the effects of free anthraquinones (FARs) extract, which was extracted from the rhubarb and purified by macroporous resin DM130 with gradient mixtures of ethanol/water as the lelution solvents, in high glucose-cultured glomerular mesangial cells (MCs). The cell proliferation was determined by CCK-8 assay, the levels of TGF-β1, CTGF, ColIV and FN proteins in the supernatant of MCs were measured by ELISA assays, and the mRNA levels of these four genes were detected by RT-PCR. The results showed that the increased proliferation of MCs, the mRNA levels and protein expression of TGF-β1, CTGF, ColIV and FN induced by high glucose were inhibited after the treatment with the FARs extract. This indicated that FARs extract could inhibit cell proliferation and the expression of main extracellular matrix induced by high glucose in MCs. The FARs extract exhibited potential values for prophylaxis and therapy of DN.

Exploration on Damage Mechanism and Equivalent Damage Model of High Arch Dams under Earthquakes

Jianyun Chen,Xiangyu Cao,Qiang Xu,Jing Li 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.4

Damage identification technology of high arch dams has been well developed under strong earthquakes. However, the failure mechanism of high arch dams at different locations remains unclear. For this purpose, a series of deterministic dynamic analyses for a 3D 289-m-high arch dam based on concrete damaged plasticity constitutive model are performed. The failure mechanism at different locations of high arch dams under earthquakes is studied systematically. According to the sensitivity of different indicators to different damaged positions, an effective sensor placement scheme is proposed to assess the seismic reliability in practical engineering. Furthermore, a new effective equivalent damage model based on prior information is proposed, which can improve the efficiency of damage identification, as well as solving the problem that the real damage is concealed by homogenizing the damage in a pre-set region. The results provide suggestions and theoretical basis for sensor placement in actual monitoring of high arch dams; and the equivalent damage model provides a new solution to balance the efficiency and accuracy of damage identification.

Seismic Damage Indexes of a High Arch Dam Based on the Monolith

Jianyun Chen,Lijun Qin,Qiang Xu,Jing Li 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.7

To assess safety of a dam in an earthquake, establishing the performance evaluation index of the arch dam is significant. The finite-element model based on the 289 m-high Baihetan Dam in Southwest China is taken as a case for seismic response analysis, in which the radiation damping of infinite foundation and opening of contraction joints are taken into consideration. Using the incremental dynamic analysis (IDA) approach, a three-component earthquake scaled to 12 increasing intensity levels is applied to the model, and the seismic response of the model is analysed. In this paper, it is proposed to study the damage based on the monolith, so the monolith damage volume ratio and monolith damage area ratio are suggested to determine the most severe part of the dam. Meanwhile, to help identifying the damage of the dam, the response results of contraction joints are extracted to analyse the potential relationship between the proposed indexes, providing a reference for exploring new detection indexes and damage evaluation indexes. In order to explore the possibility of the simplified layout of dam damage monitoring, the dam body is partitioned according to the results of monolith damage development.

Numerical Simulation Analysis of Damage Mode of Concrete Gravity Dam under Close-in Explosion

Jianyun Chen,Xiaopeng Liu,Qiang Xu 대한토목학회 2017 KSCE JOURNAL OF CIVIL ENGINEERING Vol.21 No.1

The study of damage mode of a concrete gravity dam under close-in explosive impact is a critical issue to assess the dam’s antiknock security. In this paper, a fluid-structure coupled finite element numerical method with combination of Lagrangian and Eulerian algorithm is applied, taking into consideration the damage development process of the Huangdeng concrete gravity dam. The damage mode of a square reinforced concrete slab under close-in blast loads has been simulated and discussed as a comparison with real experiment to verify the feasibility of the numerical simulation. The influence of the presence of spillway tunnel on damage mode of the concrete gravity dam has been investigated. Different damage modes of the concrete gravity dam are compared and studied for different standoff distances and different detonation depths. The damage process of the dam with time course is researched. The influence of different mediums, where the explosion occurs, on the failure mode of the dam is also researched. The analysis results show that attention should be paid to the concrete gravity dam subjected to close-in upstream shallow underwater blasts. It also should be focused on that the presence of spillway tunnel can affect the damage result of the dam.

Nonlinear Wave and Currents Loads Analysis of Offshore Wind Turbines

Xiaobo Chen,Jing Li,Jianyun Chen 대한토목학회 2014 KSCE JOURNAL OF CIVIL ENGINEERING Vol.18 No.6

In recent years, the offshore wind energy has increased in popularity due to the demand for renewable energy. Being built in a verydynamic environment, the nonlinear wave and current loads on offshore wind turbines are required to be investigated for design. Thewave and current kinematics were calculated by the model that the wave propagates on a linear shear current which was developedby Dalrymple based on the stream function wave theory. The wave and current loads were calculated based on the Morison equation. For the engineering application purpose, some problems such that the wave and current kinematics models, the wave and currentcombination schemes in statistics, and the free surface’s influences on wave and current loads were discussed. At the end, thenonlinear results were compared with linear results to verify the calculation correctness.

Effects of Free Anthraquinones Extract from the Rhubarb on Cell Proliferation and Accumulation of Extracellular Matrix in High Glucose Cultured-Mesangial Cells

Wang, Jianyun,Fang, Hui,Dong, Bingzheng,Wang, Dongdong,Li, Yan,Chen, Xiao,Chen, Lijuan,Wei, Tong,Wei, Qunli The Korean Society of Pharmacology 2015 The Korean Journal of Physiology & Pharmacology Vol.19 No.6

Diabetic nephropathy (DN) is the leading cause of end-stage failure of the kidney, but the efficacy of currently available strategies for the prevention of DN remains unsatisfactory. In this study, we investigated the effects of free anthraquinones (FARs) extract, which was extracted from the rhubarb and purified by macroporous resin DM130 with gradient mixtures of ethanol/water as the lelution solvents, in high glucose-cultured glomerular mesangial cells (MCs). The cell proliferation was determined by CCK-8 assay, the levels of TGF-${\beta}1$, CTGF, ColIV and FN proteins in the supernatant of MCs were measured by ELISA assays, and the mRNA levels of these four genes were detected by RT-PCR. The results showed that the increased proliferation of MCs, the mRNA levels and protein expression of TGF-${\beta}1$, CTGF, ColIV and FN induced by high glucose were inhibited after the treatment with the FARs extract. This indicated that FARs extract could inhibit cell proliferation and the expression of main extracellular matrix induced by high glucose in MCs. The FARs extract exhibited potential values for prophylaxis and therapy of DN.

Implementation of Physical Platform for Networked Control Systems based on GPC

Chen Zaiping,Wu Fan,Ni Jianyun,Jia Chao 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10

The performance of a Networked Control System is affected by transmission time delays in the communications network. In order to prove effectiveness of NCS algorithm design, experiment must be carried out. Therefore design and implementation of NCS hardware simulation platform is necessary. In this paper, NCS hardware simulation platform connects three computers, first as an object, second as a controller, third as network congestion Data Sources. The third computer used as the resource of data blocking can realize the network time delay through the transmission of Multicast. Finally, experiment in the NCS hardware experiment platform verify the effectiveness of the theory method proposed in this paper.

Implementation of Embedded System for Ethernet/IP Protocol

CHEN Zaiping,SHAO Xia,JIA Chao,NI Jianyun 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10

Based on the Ethernet/IP protocol and communication interfaces with embedded microprocessor platform for analysis, discuss the advantages and disadvantages of the typical variety of hardware platforms and operating systems deeply, propose the most suitable options to realize the EtherNet/IP protocol data communications interface hardware. The experimental tests confirmed the validity of the proposed approach.

One-pot synthesis of electron-acceptor composite enables efficient fullerene-free ternary organic solar cells

Zhang, Jianyun,Liu, Wenrui,Chen, Shanshan,Xu, Shengjie,Yang, Changduk,Zhu, Xiaozhang The Royal Society of Chemistry 2018 Journal of Materials Chemistry A Vol.6 No.45

<P>An effective strategy of “synthesizing an electron-acceptor composite (ZITI-m) by one-pot reaction” to improve photovoltaic performance is proposed. The ZITI-m composite is synthesized by one-pot Knoevenagel reaction of ZIT-2CHO and a mixture of INCN-2F and I-a, from which asymmetrical ZITI-3F and symmetrical ZITI-4F are separated. J71:ZITI-m-based OSC shows the highest PCE of 13.65 ± 0.13% with remarkable PCE of 13.85% than those based on J71:ZITI-3F (12.97 ± 0.15%) and J71:ZITI-4F (13.02 ± 0.13%) blends. The effect of acceptor ratio in the composite on OPV performance indicates that all PCEs of composite system are higher than those of the materials based on pure acceptors with a broad composition tolerance, which is ascribed to the synergistic effect of the ternary blend system.</P>

Research on the Influence Factors of Thermal Cracking in Mass Concrete by Model Experiments

Xianzheng Yu,Jianyun Chen,Qiang Xu,Zhi Zhou 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.8

Thermal cracking has significant impact on the safety and durability of mass concrete. This paper aims to find the reason forcracking of a prototype bridge structure and consider which influencing factors are the most important. For this model experimentson mass concrete in bridge structures were designed and conducted and thermally cracking was induced. Fiber bragg grating sensorswere used to monitor the temperature and strain within concrete in real time, as well as the strain around the embedded cooling pipes. Thermal properties of concrete were deduced from these monitoring data and then used in the numerical analyses to verity thecalculation accuracy. In the context of engineering practice, the primary influencing factors on the cracking of mass concrete wereinvestigated by numerical analyses. The optimized temperature control methods were proposed to prevent the occurrence of thermalcracking of mass concrete.