Safe Range of Retaining Pressure for Three-dimensional Face of Pressurized Tunnels based on Limit Analysis and Reliability Method

Jiahua Zhang,Wei-jun Wang,Dao-bing Zhang,Biao Zhang,Fei Meng 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.11

Based on the existing research, the 3D active and passive failure mechanisms of a pressurized tunnel face are constructed. The nonlinear failure criterion is introduced into the limit analysis by virtue of the tangent technique. By neglecting the randomness of soil parameters, the collapse pressure of active failure and the blowout pressure of passive failure, namely the lower and upper limit of retaining pressure, are obtained along with the failure ranges. On account of the randomness of soil properties, a three-dimensional stochastic model of a pressurized tunnel face is established, and the optimal range of retaining pressure of the pressurized tunnel face is presented on the basis of the multiple correlated failure modes. Taking Changsha Metro Line 2 as an example, the good agreement of the measured data in situ with the reasonable range of retaining pressure verifies the presented method. The combination of limit analysis and reliability method can provide more scientific and reasonable support parameters for the construction of pressurized tunnels in the future.

Comparative study on the structures and properties of organo-montmorillonite and organo-palygorskite in oil-based drilling fluids

Zepeng Zhang,Guanzheng Zhuang,Maguy Jaber,Jiahua Gao,Shanmao Peng 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.56 No.-

This work aims to reveal the differences of structures and properties between organo-montmorillonite(OMt) and organo-palygorskite (OPal) in oil-based drillingfluids. Organoclays are prepared in aqueoussolution and characterized by XRD, SEM and TEM. Surfactants can intercalate into the interlayer space ofMt while they coat only on the palygorskite surface. OMt can form “house of cards” structure while OPalforms “haystack” structure. Temperature rising promotes network structure and rheological properties oforganoclays in oil. The decline of rheological properties at high temperature is mainly caused by thedissolution of surfactants into oil, instead of thermal decomposition.

Research on Comparison and Evolution Mobile Customers Participate Type in Open Innovation

Zhen Zhang,He Junhui,Jiahua Li 보안공학연구지원센터 2014 International Journal of Multimedia and Ubiquitous Vol.9 No.8

SrAl₁₂O₁₉:Pr³⁺ nanodisks and nanoplates: New processing technique and photon cascade emission

Nie, Zhaogang,Zhang, Jiahua,Zhang, Xia,Lim, Ki-Soo Cambridge University Press (Materials Research Soc 2009 Journal of materials research Vol.24 No.5

<P>High-quality SrAl12O19:Pr<SUP>3+</SUP> nanodisks and nanoplates were fabricated via a new processing technique based on a modified polymer steric entrapment method. Serious agglomeration and large particle size distribution of final products, which usually occurred in the conventional method, were eliminated completely. The effects of new synthetic processes on the morphology, crystallization, and yield of products and the relevant mechanisms were discussed. As far as we know, SrAl12O19:Pr<SUP>3+</SUP> nanodisks with mean diameter ∼60 nm and thickness between 5 and 10 nm were successfully synthesized for the first time by this low-cost technique. The new synthetic method may provide a general route to synthesize other refractory mixed-oxide nanocrystals. Photon cascade emission involving transitions <SUP>1</SUP>S0-<SUP>1</SUP>I6 followed by <SUP>3</SUP>P0-<SUP>3</SUP>H4 in SrAl12O19:1% Pr<SUP>3+</SUP> nanodisks was investigated. Size-effect-induced blue shift of the 4<I>f</I>5<I>d</I> states of Pr<SUP>3+</SUP> was observed in SrAl12O19:1% Pr<SUP>3+</SUP> nanodisks, in which the quantum efficiency was preserved, as in the bulk counterparts.</P>

Polymorphisms in Exon 2 of MHC Class II DRB3 Gene of 10 Domestic Goats in Southwest China

Zhao, Yongju,Xu, Huizhong,Shi, Lixiang,Zhang, Jiahua Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.6

Polymorphism of the second exon of the caprine leukocyte antigen-DRB3 gene (CLA-$DRB3^*02$) was investigated in this study. The 285 bp PCR product of 258 individuals from 10 domestic goat breeds in Southwest China was digested with restriction endonucleases PstI and HaeIII and then genotyped. Three alleles and 4 restriction digestion profiles were distinguished by digestion of the PCR fragment by PstI, and 8 alleles and 13 genotypes by HaeIII. For HaeIII restriction enzyme sites, the Chi-square ($X^2$) test showed that all goat breeds in this study did not fit with the Hardy-Weinberg equilibrium (p<0.01 or p<0.05). The highly polymorphic nature of CLA-$DRB3^*02$ was demonstrated and the ranges of gene heterozygosity (He) and polymorphism information content (PIC) were 0.36-0.63 and 0.32-0.55, respectively. Clustering analysis showed that the 10 goat breeds clustered into two groups and Dazu Black goat had a close genetic relationship with Chengdu Grey, Jintang Black and Nanjiang Yellow goats.

Clinical report and genetic analysis of a novel variant in ZMIZ1 causing neurodevelopmental disorder with dysmorphic factors and distal skeletal anomalies in a Chinese family

He Liting,Wang Yao,Pan Jiahua,Guo Limin,Zhou Haoquan,Zhang Lan 한국유전학회 2024 Genes & Genomics Vol.46 No.4

Background Neurodevelopmental disorder with dysmorphic factors and distal skeletal anomalies (NEDDFSA) is a rare and phenotypically variable disorder. The zinc finger MIZ-type containing 1 gene (ZMIZ1) is a causative gene of NEDDFSA that encodes a protein inhibitor of the activated STAT-like family transcriptional regulator. Given the rarity of reported NEDDFSA cases, new phenotypes and genotypes of this disorder are still being discovered. Objective This study describes the phenotype characteristics of a Chinese NEDDFSA family caused by a novel ZMIZ1 variant. Methods We reviewed the clinical phenotype of a Chinese patient with NEDDFSA and performed whole-exome sequencing (WES) of the patient’s family. We simulated the potential biological harmfulness of the mutant protein. Plasmids were constructed and used for western blot and immunofluorescence assays to analyze protein expression levels. Results The patient was a 6-month-old male infant who exhibited dysmorphic facial features, neurodevelopmental abnormalities, congenital heart disease, and previously unreported genitourinary system anomalies. WES revealed a non-frameshift deletion variant in ZMIZ1 (NM_020338.4: c.858_875del, p.Val288_Ala293del), resulting in a structural alteration in the protein’s alanine-rich domain. Western blot and immunofluorescence assays indicated a significant decrease in the expression level of the mutant ZMIZ1 protein compared to the wild-type protein. Conclusion The clinical manifestations of this patient may be associated with the ZMIZ1 variant, and the structural alteration in the alanine-rich domain of the ZMIZ1 protein may contribute to a more complex disease phenotype. These results expand the genotype–phenotype correlation of ZMIZ1. Background Neurodevelopmental disorder with dysmorphic factors and distal skeletal anomalies (NEDDFSA) is a rare and phenotypically variable disorder. The zinc finger MIZ-type containing 1 gene (ZMIZ1) is a causative gene of NEDDFSA that encodes a protein inhibitor of the activated STAT-like family transcriptional regulator. Given the rarity of reported NEDDFSA cases, new phenotypes and genotypes of this disorder are still being discovered. Objective This study describes the phenotype characteristics of a Chinese NEDDFSA family caused by a novel ZMIZ1 variant. Methods We reviewed the clinical phenotype of a Chinese patient with NEDDFSA and performed whole-exome sequencing (WES) of the patient’s family. We simulated the potential biological harmfulness of the mutant protein. Plasmids were constructed and used for western blot and immunofluorescence assays to analyze protein expression levels. Results The patient was a 6-month-old male infant who exhibited dysmorphic facial features, neurodevelopmental abnormalities, congenital heart disease, and previously unreported genitourinary system anomalies. WES revealed a non-frameshift deletion variant in ZMIZ1 (NM_020338.4: c.858_875del, p.Val288_Ala293del), resulting in a structural alteration in the protein’s alanine-rich domain. Western blot and immunofluorescence assays indicated a significant decrease in the expression level of the mutant ZMIZ1 protein compared to the wild-type protein. Conclusion The clinical manifestations of this patient may be associated with the ZMIZ1 variant, and the structural alteration in the alanine-rich domain of the ZMIZ1 protein may contribute to a more complex disease phenotype. These results expand the genotype–phenotype correlation of ZMIZ1.

The miR-145-5p/CD36 pathway mediates PCB2-induced apoptosis in MCF-7 cells

Yuan Yuan,Caihua Xue,Qiang Wu,Mengjie Wang,Jiahua Liu,Longfei Zhang,Qianwen Xing,Jingyan Liang,Hua Wu,Zhi Chen 한국유전학회 2021 Genes & Genomics Vol.43 No.2

Background Procyanidin B2 (PCB2) can increase the levels of anti-infammatory and immune mediators. Objectives However, its molecular mechanism in human breast cancer remains unclear. This study aimed to investigate the antitumor efect of PCB2 on MCF-7 cells and to examine the underlying mechanism. Methods The fow cytometry and EdU incorporation assays were measured the PCB2-induced BMECs. The expression levels of infammatory factors and immune response genes were upregulated in MCF-7 cells, high-throughput sequencing was used to detect diferentially expressed genes in blank and PCB2-treated MCF-7 cells. Results The results showed that PCB2 induced the apoptosis of MCF-7 cells. CD36 profles were afected in MCF-7 cells. Additionally, prediction software identifed a miR-145-5p binding site in the CD36 sequence. Luciferase reporter assays and Western blot analysis were used to verify the regulatory relationships between the diferentially expressed miRNA miR145-5p and CD36. MiR-145-5p and its key target (CD36) constitute a potential miRNA-mRNA regulatory pair. Functional studies in MCF-7 cells revealed that CD36 promotes but miR-145-5p inhibits apoptosis. Conclusion Overall, these data suggest that miR-145-5p inhibits the enhancing efect of PCB2 on CD36 expression by binding CD36 and subsequently regulating apoptosis, the immune response and anti-infammatory pathways. These results provide theoretical and experimental support for the treatment of breast cancer.