Photodynamic antibacterial micro/nanofiber composite membrane with high efficiency and low resistance filtration performance for medical protective materials

Jia-Horng Lin,Lu Yang,Xianjin Hu,Haokai Peng,Haitao Ren,Ting-Ting Li,Ching-Wen Lou 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.128 No.-

Bacterial infection is one of the major threats to human health worldwide, posing a great challenge to thedevelopment of medical protective materials with efficient filtration and long-lasting antimicrobial properties. In this study, electrospinning technology and non-woven fabrics were combined to construct amicro/nano-structured photodynamic rechargeable and storable antibacterial filtration composite membrane. The filtration efficiency of the composite membrane is as high as 99.99% for 0.5–5 lm particles,and the resistance pressure drop is 85 Pa. In addition, the release of OH and H2O2 from the compositefilm reached 5326.16 lg/g and 711.93 lg/g, respectively. After 7 cycles of quenching, the charging capacityretains more than 70 % of the original. It showed good antimicrobial properties even when storedunder dark conditions for one month. The bactericidal efficiency of the composite membrane againstE. coli and S. aureus exceeded 99% under both dark and light conditions. The development of this simpleand clean micro/nanofiber membrane provides a new idea for exploring photoactive antimicrobial andfiltration materials for medical protection.

Platelet-Derived Growth Factor Receptor-α Subunit Targeting Suppresses Metastasis in Advanced Thyroid Cancer In Vitro and In Vivo

( Ching-ling Lin ),( Ming-lin Tsai ),( Yu-hsin Chen ),( Wei-ni Liu ),( Chun-yu Lin ),( Kai-wen Hsu ),( Chien-yu Huang ),( Yu-jia Chang ),( Po-li Wei ),( Shu-huey Chen ),( Li-chi Huang ),( Chia-hwa Lee 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.5

Thyroid cancer is the most common endocrine malignancy. Patients with well-differentiated thyroid cancers, such as papillary and follicular cancers, have a favorable prognosis. However, poorly differentiated thyroid cancers, such as medullary, squamous and anaplastic advanced thyroid cancers, are very aggressive and insensitive to radioiodine treatment. Thus, novel therapies that attenuate metastasis are urgently needed. We found that both PDGFC and PDGFRA are predominantly expressed in thyroid cancers and that the survival rate is significantly lower in patients with high PDGFRA expression. This finding indicates the important role of PDGF/PDGFR signaling in thyroid cancer development. Next, we established a SW579 squamous thyroid cancer cell line with 95.6% PDGFRA gene insertion and deletions (indels) through CRISPR/Cas9. Protein and invasion analysis showed a dramatic loss in EMT marker expression and metastatic ability. Furthermore, xenograft tumors derived from PDGFRA geneedited SW579 cells exhibited a minor decrease in tumor growth. However, distant lung metastasis was completely abolished upon PDGFRA gene editing, implying that PDGFRA could be an effective target to inhibit distant metastasis in advanced thyroid cancers. To translate this finding to the clinic, we used the most relevant multikinase inhibitor, imatinib, to inhibit PDGFRA signaling. The results showed that imatinib significantly suppressed cell growth, induced cell cycle arrest and cell death in SW579 cells. Our developed noninvasive apoptosis detection sensor (NIADS) indicated that imatinib induced cell apoptosis through caspase-3 activation. In conclusion, we believe that developing a specific and selective targeted therapy for PDGFRA would effectively suppress PDGFRA-mediated cancer aggressiveness in advanced thyroid cancers.

Acupuncture for ventilator-dependent patients at a hospital-based respiratory care center: A randomized controlled trial

Jia-Ming Chen,Wan-Li Chiang,Bin-Chuan Ji,Ruei-Jhe Jhang,Pei-Hsin Chen,Ya-Lun Li,Che-Ju Chang,Sung-Yen Huang,Tsung-Chieh Lee,Chia-Yun Chen,Ching-Hsiung Lin,Sheng-Hao Lin 한국한의학연구원 2023 Integrative Medicine Research Vol.12 No.4

Background: In intensive care units, mechanical ventilation is an important therapy to help patients with dysp- nea. However, long-term ventilator dependence would consume huge medical resources and increase the risk of morbidity and mortality. The aim of the study was to examine the efficacy of the acupuncture combined with western medical care on ventilator parameters in ventilator-dependent patients. Methods: In this clinical trial, 80 ventilator-dependent patients aged 20 to 80 years old were randomly assigned to acupuncture group and control group in the respiratory care center (RCC) of Changhua Christian Hospital. Besides regular medical care and therapy, participants in the acupuncture group received acupuncture therapy at the same 17 acu-points for 20 minutes once a day, a total of 12 sessions. The ventilator parameters were recorded to evaluate the respiratory efficiency for all participants. The primary outcome was rapid shallow breathing index (RSBI), and secondary outcomes were respiratory rate (RR), tidal volume (TV) and ventilation per minute (MV). Results: Though there was no significant difference in the parameter between the acupuncture group and the control group, we found the trend of decreasing RSBI in the acupuncture group. In subgroup analyses, the mean of RSBI significantly decreased 16.02 (with the SD in 60.84) in acupuncture group, while it increased 17.84 (with the SD in 39.38) in control group (p = 0.036) after 12 sessions. Conclusion: Acupuncture treatment can improve breathing ability of patients with respirator dependence in respiratory care center.

Cushion Polyurethane Sandwich Composite Structures Strengthened by a Concave-Convex Fabric Panels

Ting-Ting Li,Peiyao Liu,Bing-Chiuan Shiu,Hongyang Wang,Hao-Kai Peng,Jia-Horng Lin,Ching-Wen Lou 한국섬유공학회 2022 Fibers and polymers Vol.23 No.12

In order to improve the low velocity impact resistance, flexible PU foam composites (FPUC) with a sandwichstructure are proposed. The interlayer is composed of a concave-convex fabric structure while the top/bottom layers are madeof polyurethane foam (PU). The core is prepared as follows. Seven warp knitted spacer fabric types (WKSF) (WKSF.1-7) arelaminated with different number of layers to form cuboids, which are fixed firmly between two layers of low melting pointpolyester (LMPET) fabrics via hot melting. The effects of spacer yarn density, spacer yarn inclination angle, combination ofspacer fabrics, and number of lamination layers on the dynamic cushion performance are evaluated. With the impact energybeing 16 J, FPUs demonstrate an energy absorption level that is 19 % and 39 % greater than PU and WKSF. Furthermore,ultra-high molecular weight polyethylene fabric can effectively enhance the surface strength. Finally, the results arecomputed via theoretical prediction formulae of dynamic buffer resistance is in conformity with the experimental test results.

Effects of STF and Fiber Characteristics on Quasi-Static Stab Resistant Properties of Shear Thickening Fluid (STF)-Impregnated UHMWPE/Kevlar Composite Fabrics

Ting-Ting Li,Wenna Dai,Liwei Wu,Hao-Kai Peng,Xiayun Zhang,Bing-Chiuan Shiu,Jia-Horng Lin,Ching-Wen Lou 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2

In order to deeply explore the fiber characteristics influencing on stab resistance of shear thickening fluid (STF)-impregnated fabrics, two different weaving fabrics, ultra-high molecular weight polyethylene (UHMWPE) fabric and Kevlar fabrics are saturate the various concentrations and particle size of STFs. Result shows that, SiO2/PEG-200 blends demonstrate quick shear-thickening property, and the critical shear rate lowers to 1.2-45 s-1 with higher concentration of 75 nm SiO2. STF concentration and particle size significantly affect spike puncture resistance property, but the knife stab resistance mainly depends on fiber characteristics. Comparatively, STF-UHMWPE composite fabrics exhibit better knife stab resistance but weaker spike puncture resistance than STF-Kevlar fabrics. This study can provide an optimization for structure design of stab resistance armors in the future.

Preparation and Mechanical Property Evaluations of Puncture-Resistant Insoles Composites Reinforced by High-Modulus Filament and Thermal Bonding

Ting-Ting Li,Fei Sun,Xing Liu,Hua-Ling Wu,Ching-Wen Lou,Jia-Horng Lin 한국섬유공학회 2018 Fibers and polymers Vol.19 No.6

High-modulus PET filaments and thermal bonding are used to reinforce the puncture resistance stability of the insole composite. This study aims to discuss the influences of the amount of low-melting-point polyester fibers (LMPET) and needle densities (ND) on tensile, bursting, and quasi-static puncture resistance properties. Besides, significance of LMPET amount and ND on puncture resistance against flat-head (A), spherical-head (B), and pointed-head (C) probes are in particular investigated to simulate the diversified application environments for insoles. Research result shows that, LMPET amount significantly affects the static puncture resistance against three probes; ND only significantly influences the puncture resistance against Probe A and C. Thermal bonding significantly improves the puncture strength against Probe B with various LMPET amounts of insoles, but evidently increases the puncture resistance against Probe A and C when being punched at various ND. The amount of LMPET fibers has a positive influence on the puncture strength of insoles, and 70 wt% of LMPET provide the average static puncture resistance up to 342.6 N. The high-modulus resultant insoles have advantages of flexibility, ease of process, and bendability with a higher and more stabilized puncture resistances.

Synergistic Effects of Needle Punching and Shear-Thickening Fluid on Sandwich-Structured Composites Made of Nonwoven and Woven Fabrics

Xiayun Zhang,Ting-Ting Li,Hao-Kai Peng,Zhike Wang,Junli Huo,Ching-Wen Lou,Jia-Horng Lin 한국섬유공학회 2020 Fibers and polymers Vol.21 No.7

This study explores the influences of needle punching and a shear-thickening fluid (STF) on the stab resistancesandwich-structured composites made of nylon nonwoven and aramid fabrics. Needle punching and different treatments ofaramid fabric are conducted to obtain the composites. The effects of needle punching and different treatments of aramidfabrics on the sandwich-structured composites were examined using quasi-static puncture resistance, quasi-static stabresistance, dynamic puncture resistance, and bursting property tests. Results show that needle punching and incorporation ofSTF positively influence the stab resistance of the composites. This study provides an innovative method to reduce thenumber of lamination layers and decrease the weight.

Perforation threshold energy of carbon fiber composite laminates

Shun-Fa Hwang,Jia-Ching Li,Ching-Ping Mao 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.43 No.2

Two carbon fiber composite laminates, [0/90]2S and [0/+45/90/−45]S, were considered in this work to find out the perforation threshold energy to complete the perforation process and the corresponding maximum contact force. Explicit finite element commercial software, LS-DYNA, was used to predict these values. According to the simulation results, these two types of composite laminates were tested by using a vertical drop-weight testing machine. After testing, the damage condition of these specimens were observed and compared with the results from finite element analysis. The testing results indicate that the perforation threshold energy is 6 Joules for [0/90]2S and 7 Joules for [0/+45/90/−45]S, which is in good agreement with the simulation results. Also, the maximum contact force at the case of perforation threshold energy is the lowest as compared to the maximum contact forces occurring at the impact energy that is larger or less than the perforation threshold energy.

Stab/Puncture Resistance Performance of Needle Punched Nonwoven Fabrics: Effects of Filament Reinforcement and Thermal Bonding

Xiayun Zhang,Ting-Ting Li,Ting-Ting Li,Hao-Kai Peng,Zhike Wang,Jia-Horng Lin,Ching-Wen Lou 한국섬유공학회 2022 Fibers and polymers Vol.23 No.8

This paper fabricated two types stab-resistant nonwoven fabric and the stab/puncture resistance of nonwoven werestudied. Nylon fibers, Kevlar fibers, and two types of low-melting point fibers-low-melting point polyester (LPET) fibers andpolypropylene(PP) fibers-are made into PP/nylon/Kevlar and LPET/nylon/Kevlar nonwoven fabrics. Needle punching andthermal bonding technology are used to made stab-resistant nonwoven fabric, thereby examining the influence of lowmeltingpoint fibers content (i.e. LPET or PP fibers) and bonding mode with other fibers on the stab/puncture resistanceproperty of nonwoven fabrics. The quasi-static stab resistance and bursting properties were compared between nonwovenfabrics and nonwoven fabric of PET filament reinforced nonwovens. The test results show that PET filament reinforcementhas a positive influence on the mechanical properties of composite nonwoven fabrics, especially puncture/stab resistanceperformance.

Perforation threshold energy of carbon fiber composite laminates

Hwang, Shun-Fa,Li, Jia-Ching,Mao, Ching-Ping Techno-Press 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.43 No.2

Two carbon fiber composite laminates, $[0/90]_{2S}$ and $[0/+45/90/-45]_S$, were considered in this work to find out the perforation threshold energy to complete the perforation process and the corresponding maximum contact force. Explicit finite element commercial software, LS-DYNA, was used to predict these values. According to the simulation results, these two types of composite laminates were tested by using a vertical drop-weight testing machine. After testing, the damage condition of these specimens were observed and compared with the results from finite element analysis. The testing results indicate that the perforation threshold energy is 6 Joules for $[0/90]_{2S}$ and 7 Joules for $[0/+45/90/-45]_S$, which is in good agreement with the simulation results. Also, the maximum contact force at the case of perforation threshold energy is the lowest as compared to the maximum contact forces occurring at the impact energy that is larger or less than the perforation threshold energy.