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Jeong Sheop Shin(申正燮) 한국육종학회 1992 한국육종학회지 Vol.24 No.3
Genomic single or low copy number DNA clones from random genomic DNA libraries using the plasmid vector pBR322 and the phage λgtll were constructed using DNA from a barley cultivar, Sacheon #6. This work was done to develop the barley RFLP DNA markers and to saturate the linkage groups. In the initial screening, 25 clones, which were expected to have single or low-copy sequence, were selected from 1,200 bacterial colonies and 50 genetically unique phage plaques. Of these, 10 clones showed polymorphisms. To do these works, DNAs that were isolated from the barley lines and cultivars, MMS, Apex, Olbori, Sacheon #6, were digested with 3 restriction enzymes, Bam HI, Hind Ⅲ and Eco RI to prepare the genomic blot. Chemiluminescence as well as nick-translation was utilized to label the probe.
Jeong Sheop Shin(申正燮),Seung Jae Lee(李承再),Kuen Woo Park(朴權瑀) 한국육종학회 1995 한국육종학회지 Vol.27 No.1
A total of 39 genotypes of watermelon were chosen to identify the frequency of RAPD polymorphisms and to group into sub-populations. Of the 15 primers tested, 14 primers except primer 275 showed the polymorphic pattern. A total of 162 bands were generated across all 39 genotypes. Among them, 100(62%) were polymorphic and the remaining 62(38%) were monomorphic. The mean 1-F(genetic distance) was 0.114, and this value corresponded to the mean value of 0.117 detected in 36 genotypes of sorghum. The hightest value was 0.309 and the smallest one was 0.012. The 100 polymorphic fragments of total 162 bands were proved to be very reliable and confident, and selected as useful polymorphic markers. The mean value in this comparison was 0.24 and the highest onewas 0.69. Eight RAPD markers were expected to be utilized in the unique variety discrimination of eight watermelon genotypes. Using DNA polymorphisms, the two major clusters were resolved and one of these was subdivided into three cluster. Each sub-group were characterized and identified again with morphological and genetic characteristics of respective genotype.
NaCl, 한발 및 온도 처리에 따른 유묘기 수도의 폴리펩티드 속성의 비교분석
임금춘,정영상,신정섭,Lim, Gum-Chun,Jung, Yeoung-Sang,Shin, Jeong-Sheop 한국응용생명화학회 1992 Applied Biological Chemistry (Appl Biol Chem) Vol.35 No.6
식물은 자극이 큰 환경적 stress에 반응 외견상의 변모 뿐 아니라 내적으로 생리적 생화학적인 변화가 있게 되며, 특히 체내의 단백질 합성계는 그러한 stress에 더욱 급격하게 반응한다. 여러가지 stress하에서 유묘기 벼의 단백질 수준이 어떻게 변화되는 가를 일차적으로 조사하기 위하여 NaCl, 한발 및 온도를 각각 달리 처리하여 이에 따른 폴리펩티드의 변이 양상을 전기영동에 의하여 비교하여 보았다. 환경 변화에 내성이 다소 강한 벼의 경우에도 stress 처리에 따라 다수의 폴리펩티드가 변화되었으며, 새로운 폴리펩티드의 생성도 관찰 되었다. 또한 기존의 1차 대사과정을 위한 단백질의 감소도 각각의 처리에 따라서 달리 표현되는 것이 확인되었다. Plants are altered not only in the outward appearance but also in their physiological and biochemical properties with reaction to the environmental stresses; particularly, the biosynthetic system of protein in situ rapidly responds to this. In order to investigate the change of properties of polypeptides in rice plants induced by several stresses, the seedlings were subjected to exposure to NaCl, drought, and low and high temperatures, respectively, and then some aspects of polypeptide variations were compared. Without exception, the rice plant, which is somewhat tolerant to environmental change, shows the alteration in several polypeptides. Moreover, newly synthesized polypeptides were observed in response to stresses. The existing proteins for the primary metabolic pathways were markedly decreased as each treatment progressed.
벼의 arginine decarboxylase DNA clone 의 재조합 및 염기서열 분석
홍성회(Sung Hoi Hong),신정섭(Jeong Sheop Shin),정지웅(Ji Ung Jeung),옥승한(Sung Han Ok) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.2
Arginine decarboxylase (ADC) is the first enzyme in one of the two pathways of diamine putrescine biosynthesis in plants. The genes encoding ADC have previously been cloned from Escherichia coli, oat and tomato genome. Two degenerate oligonucleotides (17-mer) corresponding to two conserved regions of ADC were used as primers in polymerase chain reaction of rice (Oryza sativa L.) genomic DNA, and an approximately 1.0 kbp fragment was obtained. This amplified PCR product showed an open reading frame which contains 1,022 by of nucleotide sequences. This PCR product was cloned into pGEM-originated T vector anti the short 500 by Pst1 digested fragment was subcloned into pGEM-3zf(+/-) vectors to facilitate sequencing. The nucleotide sequence of this PCR product showed about 74% and 70% identity with the same regions of the oat and tomato ADC cDNA sequences, respectively. The predicted .amino acid sequence exhibited 45% and 62% identity with oat and tomato ADC polypeptide fragments, respectively. The sequence similarities of 34%, 47%n and 38% were previously reported in oat and E. coli, tomato ;md oat, and tomato and E. coli ADC amino acids, respectively. Therefore, similarities and identities between rice and oat or tomato are remarkably higher than those others of the previous reports. In the highly conserved regions in both the amino acid sequence and spacing regions among the sequences of these three, rice ADC open reading frame also has the exactly same regions with the striking similarity. RNA blot analysis showed that ADC is expressed as a transcript of approximately 2.5 kbp in the rice seedling leaf tissues.