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( Sang Chul Shin ),( Jong Yul Roh ),( Hee Jin Shim ),( Soon Kee Kim ),( Chul Su Kim ),( Il Kwon Park ),( Mun Jang Jeon ),( Yeon HO Je ) 한국잠사학회 2005 International Journal of Industrial Entomology Vol.10 No.2
Beauveria bassiana F-101, which has high toxicity toward Acantholyda parki as well as Thecodiplosis japonensis, was an isolate to develop an alternative control system against the major forest pests. Up to now, in B. bassiana, only one pr1 gene has been isolated and characterized. Therefore, we here reported the identification of a prl-like gene, which would be a factor of toxicity from B. bassiana F-101. The oligonucleotides for the amplification of the prl-like gene, were chosen based on the conserved regions of the subtilisin family enzymes, prl genes of B. bassiana and Metarhizium anisopliae, and proteinase K of Tritirachium album. The cloned PCR fragment had 1111bp including 52bp intron. The deduced Prl-like peptide showed a low identity with Prls of entomopathogenic fungi such as B. bassiana Prl (BbPrl) and M. anisopliae Prl (MaPrl) as well as the proteinase K of T. album (TaPrK). Instead, the deduced peptide had a substantially high amino acid sequence identity (>65%) with the serine proteases of Magnaporthe grisea (MgSPM1) and Podospora anserina (PaPspA). These results, therefore, appear to suggest that the putative Prl-like peptide of B. bassiana F-101 belongs to the subtilisin-like serine protease family and may be a novel gene.
Jeon, Sang-Je,Park, Jeong-Kil,Kim, Hyeon-Cheol,Woo, Sung-Gwan,Kim, Kwang-Hoon,Son, Kwon,Hur, Bock 대한치과보존학회 2006 Restorative Dentistry & Endodontics Vol.31 No.5
본 연구의 목적은 네 가지 형태의 비우식성 치경부 병소에 과다한 교합하중을 가했을 때 각 와동에 나타나는 응력분포를 3차원적으로 조사하고자 하였다. 임상적으로 많이 관찰되는 다양한 형태의 병소 중 4가지 형태의 서로 다른 병소를 대표적으로 선택하여 발치된 상악 제2소구치에 3차원 유한요소 모형을 제작하였다. 형성된 모형에 협측교두와 설측교두에 500 N의 하중을 가한 후 치경부병소 첨부와 수직 절단면의 주 응력을 분석하여 다음과 같은 결과를 얻었다. 1. 서로 다른 네 가지 형태의 와동에서 응력분포 양상은 비슷했지만 응력의 크기가 서로 달랐다. 2. 최대치 응력은 근심협측 우각부에서 나타났으며, 또한 병소의 첨부에 응력의 집중을 보였다. 3. 하중 A에서는 주된 응력이 압축 응력이었고 하중 B에서는 주된 응력이 인장 응력이었다. 4. 이러한 하중 하에서 수복치료를 하지 않으면 와동의 크기는 점차 커지고 치아구조에는 유해하게 작용하리라 생각된다. The objective of this study was to investigate the effect of excessive occlusal loading on stress distribution on four type of cervical lesion, using a three dimensional finite element analysis (3D FEA). The extracted maxillary second premolar was scanned serially with Micro-CT. The 3D images were processed by 3D-DOCTOR. ANSYS was used to mesh and analyze 3D FE model. Four different lesion configurations representative of the various types observed clinically for teeth were studied. A static point load of 500N was applied to the buccal and lingual cusp (Load A and B). The principal stresses in lesion apex, and vertical sectioned margin of cervical wall were analyzed. The results were as follows 1. The patterns of stress distribution were similar but the magnitude was different in four types of lesion. 2. The peak stress was observed at mesial corner and also stresses concentrated at lesion apex. 3. The compressive stress under load A and the tensile stress under load B were dominant stress. 4. Under the load, lesion can be increased and harmful to tooth structure unless restored.
Jeon, Jin Sue,Ahn, Jun Hyong,Moon, Youn-joo,Cho, Won-Sang,Son, Young-Je,Kim, Seung-Ki,Wang, Kyu-Chang,Bang, Jae Seung,Kang, Hyun-Seung,Kim, Jeong Eun,Oh, Chang Wan BMJ Publishing Group Ltd 2014 Journal of neurology, neurosurgery and psychiatry Vol.85 No.7
<P><B>Objective</B></P><P>The elevation of cellular retinoic acid-binding protein-I (CRABP-I) has been suggested as a candidate in the pathogenesis of paediatric moyamoya disease (MMD). However, few studies have addressed CRABP-I in adult onset MMD. The aim of this study was to examine the expression of CRABP-I in the cerebrospinal fluid (CSF) of adult onset MMD, and to evaluate its association with clinical presentation and postoperative haemodynamic change.</P><P><B>Methods</B></P><P>This study examined the CSF from 103 patients: bilateral MMD, n=58 (56.3%); unilateral MMD, n=19 (18.4%); atherosclerotic cerebrovascular disease (ACVD), n=21 (20.4%); and control group, n=5 (4.9%). The intensity of CRABP-I was confirmed by western blotting and expressed as the median (25th–75th percentile). The differences in CRABP-I expression according to disease entity (unilateral MMD vs bilateral MMD vs ACVD), initial presenting symptoms (haemorrhage vs ischaemia) and postoperative haemodynamic change (vascular reserve in single photon emission CT and basal collateral vessels in digital subtraction angiography) were analysed.</P><P><B>Results</B></P><P>CRABP-I intensities in bilateral MMD (1.45(0.86–2.52)) were significantly higher than in unilateral MMD (0.91(0.78–1.20)) (p=0.044) or ACVD (0.85(0.66–1.11)) (p=0.004). No significant differences were noted based on the initial presenting symptoms (p=0.687). CRABP-I was not associated with improvement in vascular reserve (p=0.327), but with decrease in basal collateral vessels (p=0.023) postoperatively.</P><P><B>Conclusions</B></P><P>Higher CRABP-I in the CSF can be associated with typical bilateral MMD pathogenesis in adults. Additionally, postoperative basal collateral change may be related to the degree of CRABP-I expression.</P>
( Sang Chul Shin ),( Jong Yul Roh ),( Chul Su Kim ),( Il Kwon Park ),( Mun Jang Jeon ),( Yeon Ho Je ) 한국잠사학회 2007 International Journal of Industrial Entomology Vol.8 No.1
For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidiophores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM-T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99% and 97% to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95% to B. amorpha, 95% to B. tenella, 89% to B. vermiconia and 69% to B. alba, respectively. In addition, in comparison to different genus, it had 95% similarities to Cordyceps militaris and 91% to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.
Jeon, Bo-Young,Kim, Seung-Cheol,Je, Sungmo,Kwak, Jeongyeon,Cho, Jang-Eun,Woo, Jong-Tae,Seo, Sangkyo,Shim, Hang-Sub,Park, Byoung-Ok,Lee, Sung-Sik,Cho, Sang-Nae Elsevier 2010 Research in veterinary science Vol.88 No.3
<P><B>Abstract</B></P><P>An enzyme-linked immunosorbent assay (ELISA) using bulk tank milk samples was evaluated as a screening test for bovine tuberculosis (TB), a contagious chronic disease of cattle. An ELISA with MPB70, a major antigen of <I>Mycobacterium bovis</I> was performed using paired sets of milk and sera samples from 33 tuberculin-positive and 43 tuberculin-negative cattle. Anti-MPB70 antibodies were detected in milk samples and there was a significant correlation between seroreactivities of milk and sera samples (<I>R<SUP>2</SUP></I>=0.83). Using the tuberculin skin test as the reference test, the sensitivities of ELISA using milk and sera samples were 87.8% and 81.8%, respectively, and the specificities were 97.7% and 100%, respectively.</P><P>In the screening test using bulk tank milk samples from 931 dairy herds in Whasung, Gyeonggi-do, Korea, the positive rate for anti-MPB70 antibody was 4.5% (42/931) and the tuberculin-positive rate was 2.8% (26/931). Individual milk samples (<I>n</I>=253) were collected from randomly selected 8 problematic and 3 negative herds (positive and negative in the screening test by MPB70 ELISA using bulk tank milk samples, respectively) and tested by MPB70 milk ELISA. In the problematic herds, positive rates were 10.5% (20/190) for anti-MPB70 antibodies in milk ELISA and 2.1% (4/190) in the tuberculin skin test. More than one dairy cows were positive by milk ELISA among the problematic herds, and all tuberculin-positive dairy cows were positive in the milk ELISA. Further, no positive cows were detected in negative herds both by milk ELISA and tuberculin skin test. These results suggest that an ELISA, using bulk tank milk samples, might be a potential efficient screening test for bovine TB of dairy cows.</P>
Jeon, Sang-Ho,Park, Sunhwa,Song, Da-Hee,Hwang, Jong-yeon,Kim, Moon-su,Jo, Hun-Je,Kim, Deok-hyun,Lee, Gyeong-Mi,Kim, Ki-In,Kim, Hye-Jin,Kim, Tae-Seung,Chung, Hyen-Mi,Kim, Hyun-Koo 한국토양비료학회 2017 한국토양비료학회지 Vol.50 No.5
To establish new metal groundwater standard, 5 metals such as aluminum, chromium, iron, manganese, and selenium were evaluated by Chemical Ranking Of groundWater pollutaNts (CROWN) including possibility of exposure, toxicity, interest factor, connection standard for other media, and data reliability. 430 groundwater samples in 2013 and 2014 were collected semiannually from 110 groundwater wells and they were analyzed for selenium, manganese, iron, chromium, and aluminum. For this study, 430 groundwater samples were categorized into 3 geological distribution features, such as igneous, metamorphic, and sedimentary rock region and geological background levels were divided by pre-selection methods. For the results, the average concentrations of aluminum, chromium, iron, manganese, and selenium in 430 groundwater samples were $0.0008mg\;L^{-1}$, $0.0001mg\;L^{-1}$, $0.174mg\;L^{-1}$, $0.083mg\;L^{-1}$, and $0.0004mg\;L^{-1}$, respectively. In addition, among various geologies, average concentration of selenium was the highest in igneous rock region, average concentrations of chromium, manganese and aluminum were the greatest in sedimentary rock region, and average concentration of iron was the most high in metamorphic rock region. As a result of the geological background concentration with pre-selection method, background concentrations of selenium and aluminum in groundwater samples were the highest from sedimentary rock as $0.0010mg\;L^{-1}$ and $0.0029mg\;L^{-1}$ and background concentrations of manganese and iron in groundwater samples were the greatest from metamorphic rock as $0.460mg\;L^{-1}$ and $1.574mg\;L^{-1}$, and no chromium background concentration in groundwater samples was found from all geology.
Jeon, Jin Sue,Lee, Sang Hyung,Son, Young-Je,Chung, Young Seob The Korean Neurosurgical Society 2013 Journal of Korean neurosurgical society Vol.53 No.2
Bilateral abducens nerve palsy related to ruptured aneurysm of the anterior communicating artery (ACoA) has only been reported in four patients. Three cases were treated by surgical clipping. No report has described the clinical course of the isolated bilateral abducens nerve palsy following ruptured ACoA aneurysm obliterated with coil. A 32-year-old man was transferred to our institution after three days of diplopia, dizziness and headache after the onset of a 5-minute generalized tonic-clonic seizure. Computed tomographic angiography revealed an aneurysm of the ACoA. Magnetic resonance imaging showed focal intraventricular hemorrhage without brain stem abnormalities including infarction or space-occupying lesion. Endovascular coil embolization was conducted to obliterate an aneurysmal sac followed by lumbar cerebrospinal fluid (CSF) drainage. Bilateral paresis of abducens nerve completely recovered 9 weeks after ictus. In conclusion, isolated bilateral abducens nerve palsy associated with ruptured ACoA aneurysm may be resolved successfully by coil embolization and lumbar CSF drainage without directly relieving cerebrospinal fluid pressure by opening Lillequist's membrane and prepontine cistern.