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고재건,김형길 제주대학교 환경연구소 1997 환경연구논문집 : 제주대 Vol.5 No.-
The social responsibility of Corporate requires that organizations consider seriously the impact of their actions on society. By the ecological environment trends, managers need to be aware of the threats and opportunities associated with important trends in the natural environment: shortage of raw materials, increased cost of energy, increased levels of pollution, changing role of government in environment protection and the heightening of consumers' sense of environment. According to the result of this survey on EMS(i.e., Environmental Management System) the level of small business in Cheju was low. In order to increase the competitive power of Cheju small business the following things are needed: environment education program for managers and employees, supporting system for introducing ISO 14000, acquirements of Environmental Labelling and strengthening of technology and administrative & financial support.
Identification of Heterodera glycines (Tylenchida; Heteroderidae) Using qPCR
Ko, Hyoung-Rai,Kang, Heonil,Park, Eun-Hyoung,Kim, Eun-Hwa,Lee, Jae-Kook The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.6
The soybean cyst nematode, Heterodera glycines, is a major plant-parasitic nematode that has caused important economic losses to Korea's soybean production. Four species of cyst nematodes, H. schachtii, H. glycines, H. trifolii, and H. sojae, all belong to schachtii group are coexist in field soil in Korea. The rapid identification of the nematode is crucial for preventing crop damage and in decision making for controlling this nematode. This study aimed to develop a species-specific primer set for quantitative PCR (qPCR) assay of H. glycines. The specific primer set (HGF1 and HGR1) for H. glycines was designed based on the cytochrome c oxidase subunit I (COI) sequence of mitochondrial DNA. After optimization, it is possible to identify the H. glycines using a qPCR assay with DNA extracted from a single cyst and single second-stage juvenile (J2). The specificity was confirmed by the absence of SYBR fluorescent signals of three other Heterodera species. A serial dilution of DNA extracted from a single cyst was obtained for the sensitivity test. The result showed that the standard curve of the test had a highly significant linearity between DNA concentration and Ct value (R2 = 0.996, slope = -3.49) and that the detection limit concentration of DNA of the primer set was 10 pg of DNA per reaction. Our findings suggested that H. glycines could be distinguished from H. sojae and other Heterodera species when a qPCR assay is used with a specific primer set.
( Jae-hyoung Song ),( Aeri Shim ),( Yeon-jeong Kim ),( Jae-hee Ahn ),( Bo-eun Kwon ),( Thuy Trang Pham ),( Jongkook Lee ),( Sun-young Chang ),( Hyun-jeong Ko ) 한국응용약물학회 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.6
Human rhinoviruses (HRV) are one of the major causes of common cold in humans and are also associated with acute asthma and bronchial illness. Heat-shock protein 90 (Hsp90), a molecular chaperone, is an important host factor for the replication of single-strand RNA viruses. In the current study, we examined the effect of the Hsp90 inhibitor pochonin D, in vitro and in vivo, using a murine model of human rhinovirus type 1B (HRV1B) infection. Our data suggested that Hsp90 inhibition significantly reduced the inflammatory cytokine production and lung damage caused by HRV1B infection. The viral titer was significantly lowered in HRV1B-infected lungs and in Hela cells upon treatment with pochonin D. Infiltration of innate immune cells including granulocytes and monocytes was also reduced in the bronchoalveolar lavage (BAL) by pochonin D treatment after HRV1B infection. Histological analysis of the lung and respiratory tract showed that pochonin D protected the mice from HRV1B infection. Collectively, our results suggest that the Hsp90 inhibitor, pochonin D, could be an attractive antiviral therapeutic for treating HRV infection.
High performance of InGaN light-emitting diodes by air-gap/GaN distributed Bragg reflectors.
Ryu, Jae Hyoung,Kim, Hee Yun,Kim, Hyun Kyu,Katharria, Yashpal Singh,Han, Nam,Kang, Ji Hye,Park, Young Jae,Han, Min,Ryu, Beo Deul,Ko, Kang Bok,Suh, Eun-Kyoung,Hong, Chang-Hee Optical Society of America 2012 Optics express Vol.20 No.9
<P>The effect of air-gap/GaN DBR structure, fabricated by selective lateral wet-etching, on InGaN light-emitting diodes (LEDs) is investigated. The air-gap/GaN DBR structures in LED acts as a light reflector, and thereby improve the light output power due to the redirection of light into escape cones on both front and back sides of the LED. At an injection current of 20 mA, the enhancement in the radiometric power as high as 1.91 times as compared to a conventional LED having no DBR structure and a far-field angle as low as 128.2° are realized with air-gap/GaN DBR structures.</P>