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( Hyunjoo Im ),( Kyung Mo Kim ),( Sang Heon Lee ),( Choong Min Ryu ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.3
Extensive use of antibiotics over recent decades has led to bacterial resistance against antibiotics, including gentamicin, one of the most effective aminoglycosides. The emergence of resistance is problematic for hospitals, since gentamicin is an important broad-spectrum antibiotic for the control of bacterial pathogens in the clinic. Previous study to identify gentamicin resistance genes from environmental samples have been conducted using culturedependent screening methods. To overcome these limitations, we employed a metagenomebased culture-independent protocol to identify gentamicin resistance genes. Through functional screening of metagenome libraries derived from soil samples, a fosmid clone was selected as it conferred strong gentamicin resistance. To identify a specific functioning gene conferring gentamicin resistance from a selected fosmid clone (35-40 kb), a shot-gun library was constructed and four shot-gun clones (2-3 kb) were selected. Further characterization of these clones revealed that they contained sequences similar to that of the RNA ligase, T4 rnlA that is known as a toxin gene. The overexpression of the rnlA-like gene in Escherichia coli increased gentamicin resistance, indicating that this toxin gene modulates this trait. The results of our metagenome library analysis suggest that the rnlA-like gene may represent a new class of gentamicin resistance genes in pathogenic bacteria. In addition, we demonstrate that the soil metagenome can provide an important resource for the identification of antibiotic resistance genes, which are valuable molecular targets in efforts to overcome antibiotic resistance.
Hyunjoo Kim,Sun Kyung Kim,Jong-Im Kim,Sunae Kim,Young Suk Jun,Keumok Park 한국융합학회 2018 한국융합학회논문지 Vol.9 No.3
본 논문은 베하스 운동프로그램의 지역사회 여성독거노인에 대한 융합적 효과를 알아보고자 시행된 비동등성 대조 군 전후설계의 유사실험연구이다. 실험군 대조군 사전사후 연구이다. 베하스 운동프로그램은 주 2회 시행되어 4주간 총 8회 실시되었고 통증, 어깨유연성, 악력, 우울과 자살생각에 대한 측정이 이루어졌다. 수집된 자료는 Independent t-test, Chi-squre test, paired t-tests, 공분산분석 (ANCOVA)으로 분석되었다. 실험군의 4주의 실험 전후비교결과 통증(t = 4.044, p = .000)과 오른쪽 어깨 유연성(t =2.192, p=.038), 왼쪽어깨유연성(t=2.568, p=.016)의 통계적으로 유의한 향상이 확인되었다. 실험군과 대조군의 그룹간 비교에서는 자살생각의 변화양상에서 통계적으로 유의한 차이가 확인되었다(F=7.32, p =.010). 본 연구를 바탕으로 베하스 운동프로그램의 지역사회 여성 독거노인에서의 신체적 정신적 건강증진에 대한 긍정적인 효과 를 확인하였다. This study was conducted to evaluate the converge effects of the Be Happy and Strong (BeHaS) exercise program on female elders living alone, using a nonequivalent control-group pretest-posttest design. The BeHaS exercise program was conducted twice each week for 4 weeks (total 8 sessions). The outcome measures included pain, shoulder flexibility, grip strength, depression, and suicidal ideation. Independent t-test, Chi-squre test, paired t-tests and ANCOVA were used for the statistical data analysis. A statistically significant improvement emerged in pain (t=4.044, p=.000) and right and left shoulder flexibility (t=2.192, p=.038 and t=2.568, p=.016) 4 weeks later. In a group comparison, a statistically significant difference arose in changed scores of suicidal ideation (F=7.32, p=.010) between groups. The BeHaS exercise program may effectively improve the physical and psychological health of female elders living alone.
전현주(Hyunjoo Jeon),박찬우(Chan Woo Park),임익태(Ik-Tae Im) 대한설비공학회 2009 대한설비공학회 학술발표대회논문집 Vol.2009 No.-
Flow field in a roller conveyor system, induced from the movement of a cassette in which glasses for flat panel display are loaded, is numerically studied in this paper. Contamination on the glass surface by dust particles produced from mechanical friction between roller and cassette is predicted from the analysis results of flow fields. Results show that a large swirl flow is formed under the moving cassette with constant speed. This swirl flow is confined only under the cassette because two main streams from the backward and the fan filter unit on the top ceiling are sufficiently strong. Therefore, it can be said that possibility of the contamination by the particles originated from the friction is relatively low. It is also revealed that flow direction between glass plates is changed according to the speed of the cassette movement due to the shear force of glass plates.
사회복지사의 대인관계스트레스가 직무소진과 이직의도에 미치는 영향
김현주(Hyunjoo Kim),임금옥(Geumok Im),박희서(Hwieseo Park) 한국컴퓨터정보학회 2018 韓國컴퓨터情報學會論文誌 Vol.23 No.6
The purpose of this study is to investigate the relationship between interpersonal stress and job burnout, turnover intention of social welfare worker"s in social welfare center and efficient response of welfare worker"s. In order to accomplish this study purpose, the interpersonal stress of social welfare worker"s in social welfare center as an independent variable, interpersonal stress as a dependent variable of job burnout and turnover intention, and stress coping ability as a moderating variable were selected. The causal relationship between interpersonal stress and job burnout, turnover intention and the moderating effect of stress coping ability were analyzed. As a result of the analysis, the interpersonal stress of social welfare worker"s showed a significant effect on job burnout and turnover intention. Also, moderating effects of stress copying ability were significant. Based on the results of this analysis, the theoretical implications and policy implications of this study are suggested, and the directions and limitations of this study are suggested.
Feasibility and user experience of virtual reality fashion stores
Minjung Park,Hyunjoo Im,Do Yuon Kim 한국의류학회 2018 Fashion and Textiles Vol.5 No.1
Virtual reality (VR) is a computer-simulated, realistic three-dimensional environment. VR heightens a user’s sense of being in the environment and enables the user to interact with the environment. We propose that VR can be a very cost-effective way to evaluate fashion retail store designs because of the ease of developing and adjusting the store designs as well as its ability to allow users to closely mimic the real store shopping experience. Also, these benefits create an opportunity for consumer researchers to investigate realistic shopper behaviors. In order to assess the effectiveness of using VR fashion retail store models in developing store designs and in understanding the way shoppers interact with the stores, an experiment with 40 female undergraduate students was conducted. This case study was written to share the advantage of using VR and the challenges VR uniquely presents with both fashion retailers and scholars. Implications for fashion retail store designers and managers as well as consumer researchers are discussed.
Minjung Park,Hyunjoo Im,Hye-Young Kim, 글로벌지식마케팅경영학회 2017 Global Fashion Management Conference Vol.2017 No.07
Despite the positive outcomes of brand-consumer interactions on social media documented in the literature, an important question still remains: Are active brand-consumer interactions always beneficial to luxury fashion brands? This study argues that such interactions may undermine the core perceptions of the brands by making consumers feel too close to the brands. Drawing upon construal level theory of psychological distance, the purpose of this study is to examine the negative effects of brand-consumer interactions on perceptions of luxury fashion brands (i.e., social perception, uniqueness perception, quality perception) in a social media context. Two experimental studies were conducted. The purpose of Study 1 was to test the hypothesis that luxury brands, compared to mainstream brands, will be perceived as more psychologically distant and abstract. Study 1 used a 2 (brand category: luxury vs. mainstream) x 2 (brand replicates) mixed-model design in which the brand category was a between-subject factor and the brand replicates were a within-subject factor. Fifty-nine subjects recruited from Amazon MTurk participated in the study. The results of Study 1 revealed that luxury brands are inherently psychologically distant than mainstream brands. The purpose of Study 2 was to test the impact of brand-consumer interactions (i.e., high vs. low) and the mediating role of psychological distance on the three perceptions of luxury brands (i.e., social perception, uniqueness perception, quality perception) on social media. A single factor between-subjects design was used, and a total of 74 participants were recruited from Amazon MTurk. To manipulate the level of consumer-brand interaction (high vs. low), two versions of a luxury brand’s mock Facebook pages were created. For the high interaction condition, the brand responded to consumers’ posts in a friendly way and displayed the images of user photos. For the low interaction condition, the brand did not respond to consumers’ posts and displayed no images of users. As predicted, the results showed that participants indicated lower brand perceptions when the brand’s social media page displayed a high level of interactions than a low level of interactions. Moreover, formality, a measure of psychological distance, partially mediated the relationship between brand-consumer interactions and all the three brand perceptions. The findings of this study provide empirical evidence that active consumer-brand interactions on social media do not necessarily benefit luxury fashion brands, rather they can damage consumer perceptions of the brands. This study provides important implications that luxury fashion brands should maintain a sacred distance on social media; otherwise it will undermine important perceptions of the brands such as status signaling, exclusivity, and quality.