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Keum, Hyun Ok,Kim, Hye Kwon,Rho, Se Mi,Moon, Hyoung Joon,Park, Seong Jun,Park, Bong Kyun The Korean Society of Veterinary Science 2010 大韓獸醫學會誌 Vol.50 No.2
To determine the prevalence of Escherichia (E.) coli O157 : H7 from pigs after the Hazard Analysis and Critical Control Point (HACCP) system has been applied to Korean swine farm since 2006, 291 fecal samples were tested between May and December in 2008. Four E. coli O157:non-H7 (1.4%) were isolated from 4 different non-HACCP-accredited farms and they didn't have virulent genes which can cause illness for human. Also, Clostridium (C.) perfringens, Salmonella spp. and E. coli enterotoxins were tested using multiplex PCR. The positive rate for these pathogens of non-HACCP-accredited farms was higher than that of HACCP-accredited farms, and especially in case of C. perfringens, E. coli enterotoxins LT and STa, it was statistically significant (p < 0.05). Thus, the early implementation of the HACCP program is expected to greatly contribute to the safety of livestock products as well as food hygiene.
( Hyun Joo Park ),( Keum Ok Hwang ),( Eung Soo Kim ) 한국미생물 · 생명공학회 2002 Journal of microbiology and biotechnology Vol.12 No.4
Bacterial bioluminescence has been known to be a highly valuable reporter system for its potential application as an effective and simple environmental monitoring method for toxic compounds. In this short report, we constructed a streptomycetes-Escherichia coli shuttle vector-containing bioluminescence system and evaluated its potential application for toxic compounds monitoring. The luxAB bioluminescence genes from Vibrio harveyi were cloned into a streptomycetes-E. coli shuttle vector (named pESK004) and functionally expressed in Streptomyces lividans. The recombinant S. lividans containing pESK004 exhibited an optimal bioluminescence at the optical density (OD600nm) of 0.4-0.5 and aldehyde concentration of 0.005%. When the recombinant bioluminescence streptomycetes was exposed to a toxic compound such as heavy metals, chlorinated phenols, or pesticides, the bioluminescence was decreased proportionally to the concentration of toxic compound in the assay mixture. The EC50 (effective concentration to decrease 50% of the bioluminescence prior to exposure) values in the recombinant bioluminescence streptomycetes for mercury, 2,4-dichlorophenol, and malathion were measured at 2.2 ppm, 144.0 ppm, and 82.4 ppm, respectively. The degree of sensitivity and specificity pattern toward these toxic compounds characterized in this recombinant bioluminescence streptomycetes were unique when compared with previously reported bacterial bioluminescence systems, and this revealed that a recombinant bioluminescence streptomycetes might provide an alternative or complementary system for potential environmental monitoring.
Determination of Deoxynivalenol in Cereal-Based Foods and Estimation of Dietary Exposure
Ok, Hyun Ee,Kim, Hyun Jung,Cho, Tae Yong,Oh, Keum Soon,Chun, Hyang Sook Informa UK (TaylorFrancis) 2009 Journal of toxicology and environmental health. Pa Vol.72 No.21
<P>Deoxynivalenol (DON) is a trichothecene mycotoxin. DON contamination in agricultural food staples such as wheat, barley, and maize due to Fusarium colonization is an increasing problem. In order to provide risk managers with better guidance for regulatory measures, the concentration of DON in cereal-based foods (n = 689), collected from six cities between June 2005 and August 2008, was determined. Further, dietary exposure to DON was estimated by combining data on DON concentration in these foods with their consumption rates. Among 689 samples, 272 samples (39%) were contaminated with DON. Relatively high DON concentrations were found in dried corn, with a mean concentration of 109 mug/kg (n = 74). Daily intake of DON simulated by the @Risk program was estimated to between 0.066 and 0.142 mug/kg body weight (bw)/d for males and between 0.066 and 0.144 mug/kg bw/d for females. The major contributor to DON exposure in the 50th and 95th percentile intake groups was polished rice. For each age class, young children (3-6 yr) showed the highest relative intake, with a mean intake of 0.142 mug/kg bw/d for males and 0.144 mug/kg bw/d for females. However, the estimated daily DON intake did not exceed the provisional maximum tolerable daily intake (1 mug/kg bw/d) for any age group or gender. From our risk assessment, it was concluded that the current intake of DON in South Korea may not serve as a serious health hazard.</P>