Report of tree Burspahelenchus (Nematoda: Parasitaphelenchidae) species distributed in Korea

Hyerim Han,Bo-Young Han,Yeong-Jin Chung,Sang-Chul Shin 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Pine wood nematode(PWN), Bursaphelenchus xylophilus, is a causal organism to induce pine wilt disease in many varieties of pine trees. PWN is mainly distributed in the East Asia including Japan, China, and Korea, but it was originally imported from the North America of the West. Over 70 species of Bursaphelenchus have been reported, but they are morphologically similar to each other. In Korea, only two species of Bursaphelenchus xylophilus, B. mucronatus (both Asian type and European type) have been reported however, a recent survey showed the distribution of extra species of Bursaphelenchus in dead trees. Three isolates, BSPD-1, BSPD-2, and BSPL-1, were identified as Bursaphelenchus thilandae, B. hylobianum, and B. doui, respectively, which was determined by both morphological and molecular biological characteristics. Both BSPD-1 and BSPD-2 were originally collected from Pinus densiflora in Namyangju and BSPL-1 came from Liriodendron tulipifera in Wanju. The morphology of each species were compared from the original descriptions focusing on male spicule and female tail and reproductive organ. A molecular diagnosis method, ITS-RFLP was applied to confirm morphological identification. Genomic DNA was extracted from a single individual nematode and ITS DNA was amplified by PCR. Amplified ITS was digested by 5 different restriction enzymes (Rsa I, Hae III, Msp I, Hinf I, and Alu I) and provided a discriminatory profile for different species of Bursaphelenchus. The three species, B. thilandae, B. hylobianum, and B. doui, are all unrecorded species in Korea.

Identification of Bursaphelenchus species transmitted by Monochamus alternatus and M. saltuarius in Korea

Hyerim Han,Bo Young Han,Sang-Hyeon Koh,Yeong-Jin Chung,Sang-Chul Shin 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

Pine wood nematode, Bursaphelenchus xylophilus, is a causative organism to induce pine wilt disease in many varieties of pine trees. Until 2006, Monochamus alternatus had been known as the only insect vector of pine wood nematode in Korea which targeted on Pinus densiflora (Japanese red pine) and P. thunbergii (Japanese black pine). However, pine wilt disease was also reported from Korean pine tree (Pinus koraiensis) in 2006 and we found another insect vector, M. saltuarius, was involved to transmit pine wood nematode. Both Monochamus species were confirmed to transfer pine wood nematode to their hosts but, there is no detail information about other transmitted nematode. Especially Bursaphelenchus mucronatus is common species transmitted by Monochamus species which is morphologically closed to B. xylophilus. Moreover B. mucronatus have two genotypes; one is East Asian type and the other is European type. Both genotypes of B. mucronatus were found in Korea but, the host and vector information related to the genotypes of B. mucronatus was not clear. Monochamus saltuarius was collected from three different geographical locations and nematodes were extracted and identified. For the identification of the juveniles, nematode DNA was extracted and ITS-RFLP analysis was done by PCR and gel electrophoresis. The selected enzymes were Hinf I, Alu I, Msp I, Hae III, Rsa I. Most of Bursaphelenchus species carried by M. saltuarius, which collected from pine wilt disease-free area, was determined as European type of B. mucronatus. We will compare the nematode species and genotypes carried by M. alternatus and M. saltuarius. In addition the rate of nematode carrying insect and the average number of nematode per single insect will be counted and compared.

Current status on pine wilt disease in South Korea

Hyerim Han,Jong-Kook Jung,Heejung Kim 한국농약과학회 2018 한국농약과학회 학술발표대회 논문집 Vol.2018 No.10

A Simple PCR-RFLP for Idenficiation of Bursaphelenchus spp. Collected from Korea

Hyerim Han,Bo Young Han,Yeong Jin Chung,Sang Chul Shin 한국식물병리학회 2008 Plant Pathology Journal Vol.24 No.2

Accurate identification of pine wood nematode, Bursaphelenchus xylophilus is a prerequisite to diagnose the pine wilt disease. However, a fungivorous nematode, B. mucronatus is highly similar to B. xylophilus and it is difficult to differentiate these two species by morphological features. A molecular diagnosis method, ITSRFLP was applied for the identification of B. xylophilus and B. mucronatus from Korea. Genomic DNA was extracted from a single individual nematode and ITS DNA was amplified by PCR. The size of PCR product was approximately 900 bp and the sequence data were obtained after cloning. Amplified ITS was digested by 5 different restriction enzymes (Rsa I, Hae III, Msp I, Hinf I, and Alu I) and provided a discriminatory profile for B. xylophilus and B. mucronatus. Besides, B. mucro- natus was determined to have 2 different genotypes, East Asian type and European type also clearly separated by Rsa I and Hae III digestion. European type of B. mucronatus is recently collected from Pinus koraiensis and has not been reported before. ITS sequnce data were analyzed by Restriction Mapper program and the result supported ITS-RFLP pattern. These data indicated that PCRRFLP method is an accurate and simple way for identification of Bursaphelenchus species.

PCR-RFLP Identification of Three Major Meloidogyne Species in Korea

Hyerim Han,Cho, Myoung-Rae,Jeon, Heung-Yong,Lim, Chun-Keun,Jang, Han-Ik Korean Society of Applied Entomology 2004 Journal of Asia-Pacific Entomology Vol.7 No.2

Partial mitochondrial DNA from single female or second stage juvenile (J2) of root-knot nematodes was amplified by PCR (polymerase chain reaction), and the further analysis by RFLP (restriction fragment length polymorphism) provided discriminatory profiles useful for three major Meloidogyne species, M arenaria, M incognita, and M hapla, in Korea. The nematode DNA was extracted individually in nuclease-free water. The sizes of PCR product (1.7 kb and 500 bp) and restriction patterns obtained from single female nematodes were consistent with the results from single J2 within the same species. M. hapla was easily differentiated from the two other root-knot nematode species by the size of the PCR products. A fragment of 500 bp was generated from M. hapla, while M. arenaria, and M. incognita produced an 1.7 kb fragment in PCR amplification. M arenaria could be distinguished from M. incognita by analysis of restriction enzyme digestion with HinfI or Alu I. Hinf I had no digestion site in mitochondrial DNA of M. arenaria; however, it generated 1.3 kb and 400 bp fragments in M. incognita. Alu I digestion resulted in 1 kb, 460 bp, and 250 bp fragments in M. arenaria, but showed different digestion patterns in M. incognita by generating 800 bp, 460 bp, 250 bp, and 150 bp fragments.

D3-28S rDNA Characterization of Major Pratylenchus Species Collected from Chrysanthemum in Korea

Hyerim Han,Jae-Kook Lee,Dong-Ro Choi,Man-Jong Han 한국응용곤충학회 2005 한국응용곤충학회 학술대회논문집 Vol.2005 No.05

아버지의 양육태도와 양육참여도, 양육효능감의 군집유형에 따른 유아의 사회적 유능감 연구

한혜림(Han, Hyerim) 한국생활과학회 2021 한국생활과학회 학술대회 Vol.2021 No.5

유아기 자녀를 둔 어머니의 양육효능감 관련 변인에 대한 메타회귀분석

한혜림(Han, Hyerim) 한국생활과학회 2020 한국생활과학회지 Vol.29 No.6

This study examined to create ways to improve the parenting efficacy among mothers with young children by integrating factors that affect their parenting efficacy. To achieve the purpose of this study, a total of 134 studies related to the parenting efficacy of mothers with young children were selected and used for the research analysis. The study results are as follows. First, the mother-related factors were the greatest effect concerning the mothers’ parenting efficacy among the factor classifications. Second, the effect size of each factor related to the mothers’ parenting efficacy has been demonstrated in the order of parenting stress, spouse’s involvement in parenting, social support, and child temperament. Third, the publication year was indicated as moderate concerning the relationship between parenting stress, spouse’s involvement in parenting, social support, and parenting efficacy. These findings suggested improvement in the parenting efficacy of mothers with young children, a necessity for mothers to manage child care stress in a well-coordinated manner, and explore ways to actively engage their spouses in parenting and enhance their social support. It is also necessary to promote parenting based on an understanding of one’s children.

남녀 대학생이 지각한 부모애착과 진로성숙도 간의 관계에서 심리적 독립과 자기효능감의 매개효과

한혜림(Han, Hyerim),이지민(Lee, Jimin) 한국생활과학회 2016 한국생활과학회지 Vol.25 No.5

In this study, we determined the effect of parental attachment on college students’ career maturity and the mediating effects of psychological independence and self-efficacy. Five hundred and seventy-seven college students from Daegu and Gyeongsan cities were included in the study. Data were analyzed by descriptive statistics, Pearson’s correlation, structural equational models, and Sobel test, using SPSS software ver. 18.0 and Amos software ver. 18.0. Firstly, the results indicated that parental attachment had no significant effect on career maturity. Second, psychological independence and self-efficacy mediated the negative association between parental attachment and career maturity. Additionally, male and female college students showed significant differences in the relationship between parental attachment and career maturity; psychological independence of male college students showed a greater influence, as compared to female college students; whereas, female college student’s self-efficacy showed a greater influence, as compared to male college students. The findings of the study suggested that interventions for psychological independence and self-efficacy are important to improve male and female college students’ career maturity; in addition, gender should be considered to better understand career maturity.

아버지의 양육태도와 양육참여도, 양육효능감의 군집유형에 따른 유아의 사회적 유능감 연구

한혜림(Han, Hyerim) 한국생활과학회 2021 한국생활과학회지 Vol.30 No.5

This study aimed to typify the fathers’ parenting attitude, parental participation, and parental efficacy, and to analyze the differences in infants’ social competence depending on the type of fathers’ parenting characteristics. A total of 1,274 fathers were analyzed using data from the 7th year of the Korean Children and Youth Panel Surveys, and cluster analysis and ANOVA analysis were conducted using SPSS 24.0. First, the types of fathers’ parenting characteristics were categorized as; affective parenting attitude-parenting ability (31.1%), affective parenting attitude-parent anxiety (25.4%), controlled parenting attitude-parenting ability (27.5%), and controlled parenting attitude-parent anxiety (23.6%). Second, infants overall social skills, sub-factors of argument, cooperation, and self-control all had the highest type of affective parenting attitude-parenting ability. Therefore, it is necessary for a father to be confident and efficacious as a parent and to communicate with his children compassionately to improve the infants’ social skills.