컴프리 葉의 조직배양에서의 植物體 再分化에 미치는 식물호르몬의 영향

車賢哲,崔賢美 단국대학교 1993 論文集 Vol.27 No.-

This study was conducted in order to regenerate plants from leaf explants and callus induced from the leaves of comfry (Symphytum peregrium). Calli were induced from leaf blade on MS medium supplemented with 1.0 mg/l 2,4-D and 1.0~2.0 mg/l kinetin. The shoot formation from leaf explants was initiated on MS medium supplemented with IAA in combination with kinetin. The root was also induced on MS medium supplemented 0.5-1.0 mg/l IAA and 0.1-2.0mg/l kinetin. The organogenesis from callus was formed to roots on B5 medium supplemented with kinetin, BAP, 2iP, irrespective to their kind and quantity Plantlets were regenerated from callus in B5 medium containing kinetin in combination with IAA or Containing BAP in combination with IAA. The optimum concentration of growth regulator for plant regeneration was estimated to be 2.0 mg/l BAP.

버어리種 煙草의 캘러스 生長에 미치는 植物호르몬의 影響

車賢哲 단국대학교 1987 論文集 Vol.21 No.-

The objective of this study is to establish the optimum condition, especially, phytohormone activity on callus induction and growth in Nicotiana tabacum cv. Burley 21. By culturing the pith in MS medium with various kinds and concentrations of phytohormone, the following results were obtained. 2,4-D was essential for callus induction from the pith culture in Burley 21 and 3.2μM was the most optiumu concentraction on callus growth. The activity of cytokinins on the growth of callus decreased in the order of BAP, kintin, 2iP. One of environmental factors, light seemed to have detrimental effect on callus growth. As for the optimum concentrations of BAP, 2iP and kinetin, Burly 21 had the value of 0.1,0.1∼10 and 0.3∼1μM, respectively. The fresh weight of callus increased exponentially for 35days. with 9days' lag phase, before rapidly entering a stationary phase.

내염성 담배 캘러스의 미세구조

차현철 단국대학교 1999 論文集 Vol.34 No.-

The objective of this study is to to select Na-Cl-toerant callus from the cultured tobacco(Nicotuana tabacum L. cv. BY 4) tissues and to examine the properties of their structural characteristics by comparing with the corresponding non-selected(NS) callus. Selection of salt tolerant(S) callus was conducted by subcultureing the BY 4 callus in 200 mM NaCl-containing and modified MS callus culture medium for more than two years. There were some differences in ultrastructure between the two cells. One of the most prominent differences lied on the polyphosphate body(PB) of electron-dense granules. Large PBs were abundunt in the central vacuoles in S cells but absent in NS cells. In addition to this, small membrane-bound vesiclesa were also found in the vacuoles in S cells. Probably PBs and these vesicles are attributed to the mechanism of salt tolerance by avoilding toxic effect of excess salts. Accumulation of ions in the vacuoles as bound forms with organic solutes may result in compartmentalization in the vacuoles. The level of chloroplast was also different. Both cell types did not have fully functional chloplasts. A large starch grain in NS cells and a few thylakoid membranes in S cells were typical feature in each type of the chloroplasts.

당근의 고체배양에서 체세포발생중 세포내 생화학적 변화

차현철,박희승 단국대학교 1996 論文集 Vol.30 No.-

Some important macromolecules in The cells of embryogenic(E-) and non-embryogenic(NE-) calli of car-rot(Daucus carata cv. Daepooong-ochon) were quantitatively measured periodically during culture of 5 weeks on solid medium. The transfer of the callus from the MS medium with phytohormones to phytohormone-free medium induced embyroid formation at 10 days culture. And the condents of some solutes were changed dramatically at embryoid forming time. In other words, free amino acid and soluble protein contents were reached to maximum at 8th day after culture in E-callus cells. On the contrarily reducing suger content was decreased at early time leading to minimum value at 8th day on both calli imspite of the double value in E-callus comparing with Ne-callus at that time. The higher con-tents of sugars in E-callus may contributed by energy budgets needed to initiate embryoid formation. The activity of peroxidase and its specific activity was higher in E-callus than NE-callus at embryoid forming time and DNA content was steadly increased during that time. RNA content also was increased to reach peak at 16th day in NE-callus, at 24th day in E-callus. After that, the content was decreased to the original levels. In conclusion, the metabolism in the parenchyma cells may be changed dramatically in order to initiate the embryoid formation.

웨이블릿 변환과 독립성분분석을 이용한 디지털 워터마킹

차성철,김대성,최훈,손상욱,배현덕 충북대학교 컴퓨터정보통신 연구소 2004 컴퓨터정보통신연구 Vol.12 No.2

디지털 워터마크는 디지털 자료를 모사로부터 보호하는 방법이다. 그르므로 디지털 워터마크는 파일변환, 컬러변환, 약간의 몽롱화, 압축과 같은 치리에서 뿐 아니라 인쇄, 복사, 스캐닝에 대해서도 강인해야한다. 본 논문은 워터마크 추출을 위한 키 데이터와 독립성분분석을 이용하는 알고리즘을 제안한다. 이 기법에서는 워터마크는 사람의 눈에 덜 민감한 원 영상의 웨이블릿변환 계수에 삽입된다. 그리고 독립성분분석 알고리즘은 서로 확률 분포가 다른 워터마크와 계수를 분리한다. 실험결과 워터마크가 워터마크 영상에서 사람 눈으로 인지할 수 없음을 보였으며, 워터마크 영상으로부터 키 데이터를 이용 영상이 일부 제거된 경우, 잡음이 첨가된 경우, JPEG 압축에서 워터마크의 추출이 가능함을 보였다. A digital watermark is a method to protect digital material from counterfeit. Therefore, it must be robust to withstand any processing, such as file format conversions, color conversions, slight blurring, sharpening color adjustment and compression as well as printing, copying and scanning. This paper proposed a method using key data and ICA algorithm for extraction of watermark. In this Method, we embed the watermark to the wavelet transform coefficients of original image which located in less sensitive subband to human eyes. ICA algorithm separated between watermark and coefficient that differs with probability distribution function each other. The experiment results have shown that the watermark is invisible to human eyes in watermarked image, and it is possible for watermarked image to extract the watermark with key data in various environment, such as removing, mixed noises and JPEG compressions.

耐鹽性 煙草細胞群의 選拔

車賢哲 단국대학교 1988 論文集 Vol.22 No.-

Soil salinity by irrigation is a serious problem to agriculture leading to the decrease of crop production. To overcome this diffoculty, it is essential to adapt plants to tolerare saline enviornments. The objective of this study was to select salt-tolerant cell line in tobacco through plant tissue culture techniques. The summarized results were as follows. Flue-cured tobacco(BY 4) callus was more tolerant and healthier to saline enviornment than that of air-cured (Burley 21). The ratio of D.W./F.W. in callus was elevated in the presence of increasing NaCl concentrations. Na^+ and Cl^- uptake was considerably larger in callus cultured on 200mM NaCl medium than that of NaCl-free. On the contrary, the amount of K^+ showed a slight decrease with external NaCl concentration. Fresh weight showed a steady increase for 7 weeks and subsrquently declined in all but 200mM NaCl concentration. The effect of NaCl on the regeneration of callus was negative.

상악골 급속확장술식 전후 두경부 자세와 설골위치의 변화에 관한 연구

배현철,차경석,이진우 대한치과교정학회 1997 대한치과교정학회지 Vol.27 No.4

본 연구는 상악골 급속확장술식 후 호홉변화와 연관된 두경부 자세의 변화와 이에 따른 설골 위치 변화의 양상을 알아보기 위하여 시행하였다. 평균연령이 12세 9개월이고 Hellman stage Ⅲc에서 adult stage까지의 인두강이 정상적이며 상악골 급속활장술식을 시행한 Angle씨 Ⅲ급 골격양상의 남녀환자 32명을 대상으로 하여 이들 중 상악골 급속확장술식 시행 후 두경부각도가 증가된 군을 A군으로, 감소된 군을 B군으로 분류하였고 대조군은 연구대상과 동일한 Hellman stage이며 평균연령 12세 7개월의 Angle I급 골격양상을 띠는 남녀환자 23명으로 이들을 C군으로 하여 골격양상과 인두강크기, 두경부자세, 설골 위치에 대한 항목을 연구하여 다음과 같은 결론을 얻었다. 1. 골격양상과 인두강 비교 1) A, B군의 치료 전후 비교에서, 두 군 공히 하악이 하방경사되었고 인두강 크기는 변화가 없었다. 2) 세 군간의 치료 전후를 각가 비교한 결과, A, B군의 PMV에 대한 골격 양상과 인구강의 크기는 정상이었다. 2. 두경부 자세비교 1) A, B군의 치료 전후비교에서, A군은 두경부 각도가 상방으로 B군은 두경부 각도와 하악하연이 하방으로 경사됨을 보였다. 2) 세 군간의 치료 전후를 각각 비교한 결과, 치료전에 두경부 각도가 A군은 정상적이었으며, B군은 정상보다 상방 경사짐을 보였다. 치료후에는 A, B군 모두 정상적인 두경부각도를 보였다. 3. 설골 위치 비교 1) A, B군의 치료 전후 비교에서, 두군 공히 설골장축 각도의 변화가 없었고 전후방적으로 A군은 설골이 후방위치되었으며 B군은 변화가 없었다. 수직적으로는 A군의 APHFH가 증가되었을 뿐 나머지 항목에서는 두 군 모두 변화가 없었다. 2) 세 군의 치료 전후를 각각 비교한 결과, 설골장축 각도는 A, B군 모두 정상적이었으며, 전후방적으로 치료전에 A군이 B, C군보다 설골이 전방에 위치되었으나 치료후에는 A, B군 공히 정상적 위치였었다. 설골의 수직적 위치는 치료 전후에 A, B군 모두가 정상적이었다. The present study assessed the effects of Rapid Maxillary Expansion on head posture and hyoid bone position. For this study, 32 Angle's class Ⅲ patients - hellman 3c ~ adult, mean age 12y9m - were selected and divided into two groups, A,B according to craniocervical angulation. Crantiocervical angulation Increased in Group A and decreased in Group B after the therapy. And 23 Angle's class I persons ~ same hellman state, mean age 12y7m ~ were selected for the control group. Cephalometric analysis of skeletal pattern, pharyngeal space, head posture, hyoid bone position was performed. The results were as follows, 1. Comparison of skeletal pattern and pharyngeal space 1) All two group(A,B) had Mandibular plane inclined inferiorly and no pharyngeal space change was observed after RME therapy. 2) Skeletal pattern and pharyngeal space of Group A,B were normal before and after treatment. 2. Comparison of head posture 1) Cranicoervical angulation of Group A was increased after treatment. That of Group B was decreased and mandibular plane was inclined inferiorly after treatment. 2) Before treatment, craniocervical inclination was normal in Group A but larger than normal in Group B. After treatment, all two group(A,B) had normal craniocervical angulation. 3. Comparison of hyoid bone position. 1) After treatment, long axis of hyoid in Group A,B was not changed. Antero-posteriorly, hyoid position was changed posteriorly in Group A but no change was founded in Group B after treatment. Vertically, hyoid bone position were not changed in two groups except in crease in APHFH in Group B after treatment. 2) Long axis of hyoid bone was normal in Group A, B before and after treatment. Anteroposteriorly, hyoid bone positon was more anterior than Group B, C before treatment but all the position of two groups had normal position after treatment. Vertical position of hyoid bone was normal in all two groups before and after treatment.

Studies on Structure and Function of Photosystem 2 in Oxygenic Photosynthesis

Cha, Hyeon Cheol,Tae, Gun Sik 한국식물학회 1997 Journal of Plant Biology Vol.40 No.2

Cytochrome b-559 is an integral protein of the photosystem Ⅱ (PSⅡ) reaction center from both plants and cyanobacteria. Cytochrome b-559 has the unique structure of a heme cross linked α- and β-subunit heterodimer. The stoichiometry of cytochrome b-559 (one or two copies) per the PSⅡ reaction center has been the subject of controversy and the molar ratio of the heme group to the special chlorophyII P-680 has a number of significant implications on our understanding of the functional role of cytochrome b-559, the mechanism of electron donation in PSⅡ, and the stoichiometry of the other redox-active reaction center components. In order to determine the number of the cytochrome b-559 heme in the PSⅡ reaction center of Synechocystis sp. PCC 6803, the α- and β-subunits are covalently linked by use of the molecular genetic techniques. The resulting psbEF fusion mutant was able to grow photoautotrophically, implying that the PSⅡ complexes are assembled and functional in thylakoids. This result supports the fact that there are two set of cytochrome b-559 in the PSⅡ reaction center of Synechocystis sp. PCC 6803.

Plant Regeneration and Morphology during in-Vitro Organogenesis from Heloniopsis orientalis(Thunb.) C. Tanaka

Cha, Hyeon-Cheol,Park, Hye-Jeong,Min, Byeong-Mee 한국식물학회 2002 Journal of Plant Biology Vol.45 No.1

Heloniopsis orientalis (Liliaceae) is an important horticultural crop native to Korea. Under natural conditions, germination is poor and plant growth is delayed. Therefore, we have developed a vegetative propagation method to produce plants with vigorous growth characteristics via tissue culture. Leaf tissues were cultured on MS basal media supplemented with growth regulators 2,4-D, TDZ, BA, or zeatin. The regenerated shoots were then initiated directly from leaf explants on an MS medium containing either 0.1 to 1.0㎎/L 2,4-D or 0.1 to 3.0㎎/L BA. Healthy plantlets with adventitious roots were formed on the medium supplemented with 0.1㎎/L BA. TDZ triggered callus initiation without caulogenesis or rhizogenesis, and callus formation was better on the half-strength MS medium than on the full-strength medium. After the plants were acclimatized for one month at 4℃, they were successfully transferred to soil. In addition, we used LM and SEM to investigate shoot morphogenesis at various stages of differentiation.

내염성 담배 캘러스의 광합성능과 peroxidase의 활성

차현철 ( Hyeon Cheol Cha ) 단국대학교 신소재기술연구소 2004 신소재 연구논집 Vol.12 No.2