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Feng Ding,Shuwei Zhang,Houbin Chen,Hongxiang Peng,Jiang Lu,Xinhua He,Jiechun Pan 한국유전학회 2018 Genes & Genomics Vol.40 No.12
Litchi (Litchi chinensis Sonn.) is an important subtropical fruit crop with high commercial value due to its high nutritional values and favorable tastes. However, irregular bearing attributed to unstable flowering is a major ongoing problem for litchi producers. Previous studies indicate that low-temperature is a key factor in litchi floral induction. In order to reveal the genetic and molecular mechanisms underlying the reproductive process in litchi, we had analyzed the transcriptome of buds before and after low-temperature induction using RNA-seq technology. A key flower bud differentiation associated gene, a homologue of FLORICAULA/LEAFY, was identified and named LcLFY (GenBank Accession No. KF008435). The cDNA sequence of LcLFY encodes a putative protein of 388 amino acids. To gain insight into the role of LcLFY, the temporal expression level of this gene was measured by real-time RT-PCR. LcLFY was highly expressed in flower buds and its expression correlated with the floral developmental stage. Heterologous expression of LcLFY in transgenic tobacco plants induced precocious flowering. Meantime, we investigated the sub-cellular localization of LcLFY. The LcLFY-Green fluorescent protein (GFP) fusion protein was found in the nucleus. The results suggest that LcLFY plays a pivotal role as a transcription factor in controlling the transition to flowering and in the development of floral organs in litchi.
Yifu Zhang,Min Zhou,Meijuan Fan,Chi Huang,Chongxue Chen,Yuliang Cao,Houbin Li,Xinghai Liu 한국물리학회 2011 Current Applied Physics Vol.11 No.5
V_3O_7ㆍH_2O@C core-shell structured composites have been successfully synthesized using V_3O_7ㆍH_2O nanobelts as the cores and glucose as the source of carbon in the presence of sodium lauryl sulfate (SDS). The as-obtained V_3O_7ㆍH_2O@C core-shell materials were characterized by X-ray powder diffraction (XRD),transmission electron microscopy (TEM), elemental analysis (EA), Fourier transform infrared spectroscopy (FT-IR) and Raman spectrum. The thickness of the carbon shell can be controlled by the hydrothermal reaction time and the quantity of glucose. The surfactants have great influence on fabricating V_3O_7ㆍH_2O@C core-shell composites, which have been discussed in detail. V_3O_7@C composites were subsequently obtained through thermal treatment with V_3O_7ㆍH_2O@C. The electrochemical properties of V_3O_7@C core-shell composites were studied, indicating that the discharge capacity is still 151.2 mAh/g after 45 cycles, which is better than that of pure V_3O_7ㆍH_2O nanobelts.