Index admission laparoscopic cholecystectomy for acute cholecystitis restores Gastrointestinal Quality of Life Index (GIQLI) score

Hongyan Yu,Esther Ern-Hwei Chan,Pravin Lingam,Jingwen Lee,Winston Wei Liang Woon,Jee Keem Low,Vishal G Shelat 한국간담췌외과학회 2018 Annals of hepato-biliary-pancreatic surgery Vol.22 No.1

Backgrounds/Aims: Previous studies have evaluated quality of life (QoL) in patients who underwent laparoscopic cholecystectomy (LC) for cholelithiasis. The purpose of this study was to evaluate QoL after index admission LC in patients diagnosed with acute cholecystitis (AC) using the Gastrointestinal Quality of Life Index (GIQLI) questionnaire. Methods: Patients ≥21 years admitted to Tan Tock Seng Hospital, Singapore for AC and who underwent index admission LC between February 2015 and January 2016 were evaluated using the GIQLI questionnaire preoperatively and 30 days postoperatively. Results: A total of 51 patients (26 males, 25 females) with a mean age of 60 years (24-86 years) were included. Median duration of abdominal pain at presentation was 2 days (1-21 days). 45% of patients had existing comorbidities, with diabetes mellitus being most common (33%). 31% were classified as mild AC, 59% as moderate and 10% as severe AC according to Tokyo Guideline 2013 (TG13) criteria. Post-operative complications were observed in 8 patients, including retained common bile duct stone (n=1), wound infection (n=2), bile leakage (n=2), intra-abdominal collection (n=1) and atrial fibrillation (n=2). 86% patients were well at 30 days follow-up and were discharged. A significant improvement in GIQLI score was observed postoperatively, with mean total GIQLI score increasing from 106.0±16.9 (101.7-112.1) to 120.4±18.0 (114.8-125.9) (p<0.001). Significant improvements were also observed in GIQLI subgroups of gastrointestinal symptoms, physical status, emotional status and social function status. Conclusions: Index admission LC restores QoL in patients with AC as measured by GIQLI questionnaire.

Structural properties of disordered macroporous La2O2CO3/ZnO materials prepared by a solution combusion method

Hongyan Yu,Yong Men,신은우 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.4

Disordered macroporous La2O2CO3/ZnO materials were prepared by a solution combustion method, and then their microstructures and La2O2CO3 phases were investigated as functions of La/Zn ratios and calcination temperatures. All of the materials prepared by the solution combustion method clearly showed disordered macroporous morphology whose framework was composed of ZnO and La2O2CO3 nanoparticles. A monoclinic La2O2CO3 structure was mainly formed in the disordered macroporous materials at La/Zn=1 and 2. In contrast, the conventional coprecipitation of La2O2CO3/ZnO materials dominantly formed a hexagonal La2O2CO3 phase with aggregating morphology of ZnO and La2O2CO3 nanoparticles. However, nanocrystalline sizes of ZnO (7-10 nm) and monoclinic La2O2CO3 (12-14 nm) in the disordered macroporous structure were much smaller than those (29-36 nm for ZnO and 44-58 nm for hexagonal La2O2CO3) by conventional co-precipitation. In addition, the high temperature calcination at 700 oC increased the ZnO nanocrystallite size (24 nm) in the disordered macroporous framework, with transforming La2O2- CO3 into La2O3. This result implies that the interaction between monoclinic La2O2CO3 and ZnO in the disordered macroporous structure inhibited ZnO nanoparticle agglomeration.

A newly developed vacuum leak calibration system at NIM

Hongyan YU,Jinku WANG,Yuanchao YANG,Jin YUE,Chuxin GE 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Deformation of the ink roller of an offset press based on a simplified model of ink-rubber viscoelasticity

Hongyan Chu,Ruilong Ding,Caixia Zhang,Qiang Yu 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.12

Ink transfer is achieved by the squeezing and rotation of two rollers, one of which is a roller coated with a viscoelastic rubber layer. In this study, the force caused by ink hydrodynamic pressure on the rubber roller during the rotation is considered, the viscoelasticity of ink and rubber is equivalently substituted by spring-damping unit, and a rolling squeezing theoretical model of two rollers is established to study the node deformation of the rubber roller. The validity of the theoretical model is verified by experiments. Results show that the deformation of the rubber roller is greatly affected by the roller rotational speed, rubber layer thickness, and nip width. The rotational speed and nip width have a significant influence on the stiffness– damping of the ink layer and the ink force, and the rubber layer thickness has a remarkable effect on the squeezing deformation between the rollers.

Degradation of Lignocelluloses in Rice Straw by BMC-9, a Composite Microbial System

( Hongyan Zhao ),( Hai Ru Yu ),( Xu Feng Yuan ),( Ren Zhe Piao ),( Hu Lin Li ),( Xiao Fen Wang ),( Zong Jun Cui ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.5

To evaluate the potential utility of pretreatment of raw biomass with a complex microbial system, we investigated the degradation of rice straw by BMC-9, a lignocellulose decomposition strain obtained from a biogas slurry compost environment. The degradation characteristics and corresponding changes in the bacterial community were assessed. The results showed that rapid degradation occurred from day 0 to day 9, with a peak total biomass bacterium concentration of 3.3 × 10(8) copies/ml on day 1. The pH of the fermentation broth declined initially and then increased, and the mass of rice straw decreased steadily. The highest concentrations of volatile fatty acid contents (0.291 mg/l lactic acid, 0.31 mg/l formic acid, 1.93 mg/l acetic acid, and 0.73 mg/l propionic acid) as well as the highest xylanse activity (1.79 U/ml) and carboxymethyl cellulase activity (0.37 U/ml) occurred on day 9. The greatest diversity among the microbial community also occurred on day 9, with the presence of bacteria belonging to Clostridium sp., Bacillus sp., and Geobacillus sp. Together, our results indicate that BMC-9 has a strong ability to rapidly degrade the lignocelluloses of rice straw under relatively inexpensive conditions, and the optimum fermentation time is 9 days.

Ginsenoside compound K inhibits nuclear factor-kappa B by targeting Annexin A2

Wang, Yu-Shi,Zhu, Hongyan,Li, He,Li, Yang,Zhao, Bing,Jin, Ying-Hua The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.3

Background: Ginsenoside compound K(C-K), a major metabolite of ginsenoside, exhibits anticancer activity in various cancer cells and animal models. A cell signaling study has shown that C-K inhibited nuclear factor-kappa B ($NF-{\kappa}B$) pathway in human astroglial cells and liver cancer cells. However, the molecular targets of C-K and the initiating events were not elucidated. Methods: Interaction between C-K and Annexin A2 was determined by molecular docking and thermal shift assay. HepG2 cells were treated with C-K, followed by a luciferase reporter assay for $NF-{\kappa}B$, immunofluorescence imaging for the subcellular localization of Annexin A2 and $NF-{\kappa}B$ p50 subunit, coimmunoprecipitation of Annexin A2 and $NF-{\kappa}B$ p50 subunit, and both cell viability assay and plate clone formation assay to determine the cell viability. Results: Both molecular docking and thermal shift assay positively confirmed the interaction between Annexin A2 and C-K. This interaction prevented the interaction between Annexin A2 and $NF-{\kappa}B$ p50 subunit and their nuclear colocalization, which attenuated the activation of $NF-{\kappa}B$ and the expression of its downstream genes, followed by the activation of caspase 9 and 3. In addition, the overexpression of Annexin A2-K320A, a C-K binding-deficient mutant of Annexin A2, rendered cells to resist C-K treatment, indicating that C-K exerts its cytotoxic activity mainly by targeting Annexin A2. Conclusion: This study for the first time revealed a cellular target of C-K and the molecular mechanism for its anticancer activity.

Degradation Characteristics and Metabolic Pathway of 17β-estradiol (E2) by Rhodococcus sp. DS201

Qingmiao Yu,Ping Wang,Dongbo Liu,Ruixia Gao,Huanhuan Shao,Hongyan Zhao,Zhe Ma,Dan Wang,Hongliang Huo 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.6

In this study an E2-degrading bacterium was isolated from the activated sludge of a municipal treatment plant that treats the waste from a contraceptive medicineprocessing factory in Beijing, China. Using the observed morphological and physiological features of the bacterium and 16S rRNA sequence analysis, this bacterial strain was identified as Rhodococcus sp. DS201. Using single-factor experiments and orthogonal tests, it was demonstrated that, when strain DS210 bacteria were inoculated into MM medium at an initial concentration of 1 mg/L with an initial pH of 7 and an inoculum amount of 1%, complete degradation of E2 by this strain was achieved within 3 days at 30oC. After strain DS201 had degraded the E2, several E2 metabolites were detected in the culture extracts using high-performance liquid chromatography (HPLC); they were then further identified using HPLC with tandem mass spectrometry (LC-MS/MS). Mass spectrum analysis of the E2 degradation identified the following products: pent- 4-enoic acid; 2-ethyl-3-hydroxy-6-methylcyclohexane-1- carboxylic acid; 3-(7a-methyl-1,5-dioxooctahydro-1H-inden- 4-yl) propanoic acid; and 5-hydroxy-4-(3-hydroxypropyl)- 7a-methyloctahydro-1H-inden-1-one. These products have not previously been reported as parts of a mechanism for microbial E2 degradation and were suspected to be new metabolite products. Therefore, the E2 degradation pathway by strain DS201 is proposed herein.

The enhancement of humidity sensing performance based on Eu-doped ZnO

Shuguo Yu,Hongyan Zhang,Cunchong Lin,Mingjin Bian 한국물리학회 2019 Current Applied Physics Vol.19 No.2

In this work, a high performance impedance-type humidity sensor based on Europium-doped ZnO with abundant surface oxygen vacancy defects was synthesized by sol-gel method. Response of the Eu-doped ZnO with different molar ratio were investigated by exposing them to humidity environments in wide range of 11–95% RH at room temperature. The Eu-doped ZnO (2 mol%) exhibits a three orders impedance change, along with short response/ recovery time (5 s/19 s), low hysteresis and best linearity. Complex impedance spectra indicates that dopant Eu can enhance humidity sensing performance of ZnO, which is resulted from the introduction of Eu3+ ions into ZnO structure to produce more defects of surface oxygen vacancy and more active sites on the surface of ZnO. The results show that this is a feasible method to achieve high humidity sensing performance by Eu doped ZnO, which make it a promising candidate for humidity sensing materials and broaden the use of ZnO materials.

Evaluation of Biogas Production Performance and Archaeal Microbial Dynamics of Corn Straw during Anaerobic Co-Digestion with Cattle Manure Liquid

( Benyue Zhang ),( Hongyan Zhao ),( Hairu Yu ),( Di Chen ),( Xue Li ),( Weidong Wang ),( Renzhe Piao ),( Zongjun Cui ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4

The rational utilization of crop straw as a raw material for natural gas production is of economic significance. In order to increase the efficiency of biogas production from agricultural straw, seasonal restrictions must be overcome. Therefore, the potential for biogas production via anaerobic straw digestion was assessed by exposing fresh, silage, and dry yellow corn straw to cow dung liquid extract as a nitrogen source. The characteristics of anaerobic corn straw digestion were comprehensively evaluated by measuring the pH, gas production, chemical oxygen demand, methane production, and volatile fatty acid content, as well as applying a modified Gompertz model and high-throughput sequencing technology to the resident microbial community. The efficiency of biogas production from fresh straw (433.8 ml/g) was higher than that of production from straw silage and dry yellow straw (46.55 ml/g and 68.75 ml/g, respectively). The cumulative biogas production from fresh straw, silage straw, and dry yellow straw was 365 l-1 g-1 VS, 322 l-1 g-1 VS, and 304 l-1 g-1 VS, respectively, whereas cumulative methane production was 1,426.33%, 1,351.35%, and 1,286.14%, respectively, and potential biogas production was 470.06 ml-1 g-1 VS, 461.73 ml-1 g-1 VS, and 451.76 ml-1 g-1 VS, respectively. Microbial community analysis showed that the corn straw was mainly metabolized by acetate-utilizing methanogens, with Methanosaeta as the dominant archaeal community. These findings provide important guidance to the biogas industry and farmers with respect to rational and efficient utilization of crop straw resources as material for biogas production.

Constant pressure gas flowmeter for vacuum metrology

Jinku WANG,Hongyan YU 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8