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제초 항생물질 maculosin 생산균주 Streptomyces rochei 87015 - 3 의 동정
조홍연(Hong Yon Cho),최용철(Yong Chul Choi),서형주(Hyung Joo Suh),신광순(Kwang Soon Shin),이희봉(Heui Bong Lee),권형진(Hyung Jin Kwon),김수언(Soo Un Kim) 한국응용생명화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.6
A bacterial strain, which has been shown to produce herbicidal antibiotics maculosins and phenylacetic acid, was identified as a member of Streptomyces rochei. The identification was based on morphological and physiological characteristics. This is the first bacterial strain that produces maculosins other than a fungus, Alternaria alternata.
δ-Aminolevulinic Acid 생산 광합성세균의 분리 및 배양특성
정대열,최양문,조홍연,양한철 ( Dae Yeol Cheong,Yang Mun Chol,Hong Yon Cho,Han Chul Yang ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.6
Screening, Identification and some cultural characteristics of ALA(δ-aminolevulinic acid)-producing photosynthetic bacteria were carried out for the optimal production of ALA, one of the bioherbicides. Among photosynthetic bacteria isolated from soil, marsh, pond, etc., KK-10 was the best producer of ALA and identified to be Rhodobacter capsulatus belonging to a typical group of nonsulfur purple bacteria. By addition of 15 mM LA (levulinic acid), an inhibitor of ALA dehydrase in cyclic tetrapyrrole biosynthesis, into culture broth at middle log phase of cell growths, ALA production was considerably increased to about 20-fold (28 ㎎/ℓ). The combined supplementation of glycine and succinate, each with a concentration of 30 mM also enhanced production of ALA and activity of ALA synthase to about 50-fold (73 ㎎/ℓ) and 2-fold, respectively. The isolated strain was able to produce upto 80 ㎎/ℓ under the cultural condition optimized by addition 15 mM LA into the synthetic medium at four different points starting middle log phase.
두부공업폐수에서 Rhodobacter capsulatus KK-10을 이용한 δ-Aminolevulinic Acid의 생산
정대열,최양문,조홍연,양한철 ( Dae Yeol Cheong,Yang Mun Choi,Hong Yon Cho,Han Chul Yang ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.6
The removal efficiency of COD and the production of δ-aminolevulinic acid (ALA) were concurrently investigated for both purifying the soybean curd wastewater of high BOD and utilizing the wastewater as a renewable substrate of ALA production using Rhodobacter capsulatus KK-10. Its wastewater was a favorable media for the growth of photosynthetic bacteria in terms of its environmental characteristics having COD/BOD rate of 0.98, ratio of BOD : N : P =100 : 6 : 4, BOD/N ratio of 17.2, lactic acid of 1,080 ppm. Its COD value wastewater was decreased to 94% and dry cell weight was approached to about 1.2 g/ℓ after cultivation of the photosynthetic bacteria for 4 days. By the addition of 15 mM levulinic acid (LA) into the wastewater at the middle log phase of cell growth, the amount of ALA secreted was 55 ㎎/ℓ. The ALA production was considerably increased to 114 ㎎/ℓ under the cultural condition of 15 mM supplementations of glycine and succinate with LA at the same period. Furthermore the maximum ALA production of 120 ㎎/ℓ and COD removal efficiency of 92% were accomplished in the soybean curd wastewater enriched with one addition of 15 mM LA and three serial additions 15 mM ALA precursors.
두부공업폐수에서 Rhodobacter capsulatus KK-10을 이용한 ${\delta}-Aminolevulinic$ Acid의 생산
정대열,최양문,양한철,조홍연,Cheong, Dae-Yeol,Choi, Yang-Mun,Yang, Han-Chul,Cho, Hong-Yon 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4
광합성세균을 이용한 고농도 유기폐수인 두부공업폐수의 정화처리와 동시에 두부공업폐수를 생물제초제인 ${\delta}-aminolevulinic$ acid (ALA) 생산용 대체기질로 이용할 목적으로 Rhodobacter capsulatus KK-10을 사용하여 COD 제거율과 ALA 생산능을 검토하였다. 사용된 두부공업폐수의 성상은 pH 4.2, COD/BOD와 BOD/N의 비율은 0.98 및 17.2, BOD : N : P의 함유비율은 10 : 6 : 4, 젖산 함유량 1,080 ppm 등으로 광합성세균의 생육에 적당한 조건이었다. 두부공업폐수에서 본 균주를 4일 동안 배양한 결과 약 94% COD 값의 감소와 1.2 g/l 균체생육을 보였으며 대수기 중기에서 ALA 탈수효소 저해제인 levulinic acid(LA)를 15 mM 첨가한 결과 55 mg/l의 ALA 생산량을 나타내었다. ALA의 전구물질인 succinate 및 glycine과 LA를 각각 15 mM의 농도로 동시 첨가시 최대 114 mg/l의 생산량을 보였다. 상기의 물질들을 각각 15 mM 농도로 연속 첨가한 후 ALA 생성량을 검토한 결과 LA 1회와 glycine과 succinate를 3회에 걸쳐 첨가하였을 때 120 mg/l로 가장 효과적이었으며 COD 제거율은 92%를 나타내었다. The removal efficiency of COD and the production of ${\delta}-aminolevulinic$ acid (ALA) were concurrently investigated for both purifying the soybean curd wastewater of high BOD and utilizing the wastewater as a renewable substrate of ALA production using Rhodobacter capsulatus KK-10. Its wastewater was a favorable media for the growth of photosynthetic bacteria in terms of its environmental characteristics having COD/BOD rate of 0.98, ratio of BOD : N : P=100 : 6 : 4, BOD/N ratio of 17.2, lactic acid of 1,080 ppm. Its COD value wastewater was decreased to 94% and dry cell weight was approached to about 1.2 g/l after cultivation of the photosynthetic bacteria for 4 days. By the addition of 15 mM levulinic acid (LA) into the wastewater at the middle log phase of cell growth, the amount of ALA secreted was 55 mg/l. The ALA production was considerably increased to 114 mg/l under the cultural condition of 15 mM supplementations of glycine and succinate with LA at the same period. Furthermore the maximum ALA production of 120 mg/l and COD removal efficiency of 92% were accomplished in the soybean curd wastewater enriched with one addition of 15 mM LA and three serial additions 15 mM ALA precursors.
${\delta}-Aminolevulinic$ Acid 생산 광합성세균의 분리 및 배양특성
정대열,최양문,양한철,조홍연,Cheong, Dae-Yeol,Choi, Yang-Mun,Yang, Han-Chul,Cho, Hong-Yon 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.4
광합성세균을 이용하여 생물제초제의 하나인 ${\delta}-aminolevulinic$ acid(ALA)를 생산할 목적으로 자연계로부터 ALA 생산능력이 우수한 균주를 분리하고 ALA의 최적생산에 미치는 일부 배양특성을 검토하였다. 선정균주 KK-10을 동정한 결과 홍색비유황세균에 속하는 Rhodobacter capsulatus로 판명되었다. 본 균주의 ALA 생산성을 높이기 위하여 ALA 탈수효소의 저해제인 levulinic acid(LA)를 15 mM 농도로 배양중기에서 배양액에 첨가함으로써 ALA의 생산량은 약 20배(28 mg/l) 증가되었다. ALA의 전구물질인 glycine과 succinate를 각각 30 mM 복합첨가할 때 약 50배(73 mg/l)생산량을 나타내었고 전구물질의 첨가에 의해 ALA 합성효소의 생합성량은 2배 증가하였다. 분리균주는 전구물질 함유 배지에 15 mM LA를 대수기 중기부터 4회 연속첨가함으로써 85 mg/l 균체외 ALA를 생산하였다. Screening, Identification and some cultural characteristics of ALA$({\delta}-aminolevulinic\;acid)$-producing photosynthetic bacteria were carried out for the optimal production of ALA, one of the bioherbicides. Among photosynthetic bacteria isolated from soil, marsh, pond, etc., KK-10 was the best producer of ALA and identified to be Rhodobacter capsulatus belonging to a typical group of nonsulfur purple bacteria. By addition of 15 mM LA (levulinic acid), an inhibitor of ALA dehydrase in cyclic tetrapyrrole biosynthesis, into culture broth at middle log phase of cell growths, ALA production was considerably increased to about 20-fold (28 mg/l). The combined supplementation of glycine and succinate, each with a concentration of 30 mM also enhanced production of ALA and activity of ALA synthase to about 50-fold (73 mg/l) and 2-fold, respectively. The isolated strain was able to produce upto 80 mg/l under the cultural condition optimized by addition 15 mM LA into the synthetic medium at four different points starting middle log phase.
유기폐수처리를 위한 Rhodospirillum rubrum P17 의 종균생산
조경덕(Kyung Dug Cho),강성옥(Seong Og Kang),임왕진(Wang Jin Lim),조홍연(Hong Yon Cho),양한철(Han Chul Yang) 한국응용생명화학회 1993 Applied Biological Chemistry (Appl Biol Chem) Vol.36 No.6
A photosynthetic bacterium strain P17 having high growth rate and assimilating ability of organic acids was isolated from several soil samples, which was identified as Rhodospirillum rubrum. Cultural conditions of the strain P17 were examined for the production of starter culture used in the treatment of organic waste water. The addition of organic acids mixture as carbon source containing 0.2% Na-acetate, 0.1% Na-propionate and 0.2%r Na-lactate and 0.1% of yeast extract as growth factor stimulated the cell growth. The maximal cell production was obtained at 30℃, pH 7.0, 2,500 lux of illumination and 50∼100 rpm of agitation. Under the optimal conditions of batch and fed-batch culture systems in a Jar fermentor, 5.17 g/ℓ and 7.93 g/ℓ of cells were obtained after 5 days of cultivation, respectively. In continuous culture system, the cell productivity was 0.206 g/ℓ/h at a dilution rate of 0.21 h^(-1). When R. rubrum P17 was cultivated in a soybean curd waste water, initial COD level(3,240 ㎎/ℓ) of the waste water was reduced to 250 ㎎/ℓ after 4 days of cultivation.
감잎(Diospyos kaki L.)으로부터 정제한 보체계 활성화 다당류
정영주,전혁,김경임,안정희,신동훈,홍범식,조홍연,양한철,Jung, Yung-Joo,Chun, Hyug,Kim, Kyung-Im,An, Jeung-Hee,Shin, Dong-Hoon,Hong, Bum-Shik,Cho, Hong-Yon,Yang, Han-Chul 한국식품과학회 2002 한국식품과학회지 Vol.34 No.5
감잎으로부터 항보체 활성물질을 분리정제하기 위해 감잎 (250 g)을 $100^{\circ}C$에서 3시간 동안 열수 추출하고 분자량 10 kDa membrane을 사용하여 농축한 후 ethanol 침전과 methanol 추출을 통해 조다당류(DKC)를 얻었다. 조다당류의 정제는 DEAE-Toyopearl 650C와 Bio-gel P60을 사용하여 실시하였다. 정제된 DKC-1c는 $1000\;{\mu}g/mL$의 농도에서 고전경로를 통해서는 85.4% 활성화시켰고 부경로에서는 65.1% 활성화시켰다. 정제 다당류 DKC-1c는 분자량은 66.6 kDa이고 정제도가 높은 중성다당류로써 주요 구성당은 glucose(29.0 mol.%), arabinose(24.3 mol.%), galactose(16.2 mol.%) 순으로 검출되었다. 면역전기영동을 통하여 확인한 결과 DKC-1c는 C3를 부경로에서도 C3a와 C3b로 활성화시키는 complement activator임이 확인되었다. Cold and hot water fractions of Diospyros kaki were screened to determine its anti-complementary activity. Flour of Diospyros kaki leaf (250 g) was boiled at $100^{\circ}C$ for 3 h and passed through a membrane of 10 kDa molecular weight (DK-0). DK-0 was precipitated with ethanol and refluxed with methanol to obtain the crude polysaccharide (DKC). DKC-1 was isolated by ion exchange chromatography on DEAE-Toyopearl 650C, and DKC-1c was purified from DKC-1 by size exclusion chromatography on Bio gel P-60. The anti-complementary activities of DKC-1c at $1000\;{\mu}g/mL$ were 85.4 and 61.1% via whole and alternative pathways, respectively. DKC-1c was determined as a neutral polysaccharide composed of glucose (29.0 mol.%), arabinose (24.3 mol.%), and galactose (16.2 mol.%) with the molecular weight of 66.6 kDa. Results of agarose gel immunoelectrophoresis revealed DKC-1c, as a complement activator, cleaved C3 into C3a and C3b via both pathways.