NEDD 4‐induced degradative ubiquitination of phosphatidylinositol 4‐phosphate 5‐kinase α and its implication in breast cancer cell proliferation

Tran, Mai Hoang,Seo, Eunjeong,Min, Soohong,Nguyen, Quynh&#x2010,Anh T.,Choi, Juyong,Lee, Uk&#x2010,Jin,Hong, Soon&#x2010,Sun,Kang, Hyuk,Mansukhani, Alka,Jou, Ilo,Lee, Sang Yoon John Wiley and Sons Inc. 2018 JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Vol.22 No.9

<P><B>Abstract</B></P><P>Phosphatidylinositol 4‐phosphate 5‐kinase (PIP5K) family members generate phosphatidylinositol 4,5‐bisphosphate (PIP2), a critical lipid regulator of diverse physiological processes. The PIP5K‐dependent PIP2 generation can also act upstream of the oncogenic phosphatidylinositol 3‐kinase (PI3K)/Akt pathway. Many studies have demonstrated various mechanisms of spatiotemporal regulation of PIP5K catalytic activity. However, there are few studies on regulation of PIP5K protein stability. Here, we examined potential regulation of PIP5Kα, a PIP5K isoform, via ubiquitin‐proteasome system, and its implication for breast cancer. Our results showed that the ubiquitin ligase NEDD4 (neural precursor cell expressed, developmentally down‐regulated gene 4) mediated ubiquitination and proteasomal degradation of PIP5Kα, consequently reducing plasma membrane PIP2 level. NEDD4 interacted with the C‐terminal region and ubiquitinated the N‐terminal lysine 88 in PIP5Kα. In addition, PIP5Kα gene disruption inhibited epidermal growth factor (EGF)‐induced Akt activation and caused significant proliferation defect in breast cancer cells. Notably, PIP5Kα K88R mutant that was resistant to NEDD4‐mediated ubiquitination and degradation showed more potentiating effects on Akt activation by EGF and cell proliferation than wild‐type PIP5Kα. Collectively, these results suggest that PIP5Kα is a novel degradative substrate of NEDD4 and that the PIP5Kα‐dependent PIP2 pool contributing to breast cancer cell proliferation through PI3K/Akt activation is negatively controlled by NEDD4.</P>

The synergistic effects of clopidogrel with montelukast may be beneficial for asthma treatment

Trinh, Hoang Kim Tu,Nguyen, Thuy Van Thao,Choi, Youngwoo,Park, Hae&#x2010,Sim,Shin, Yoo Seob John Wiley and Sons Inc. 2019 JOURNAL OF CELLULAR AND MOLECULAR MEDICINE Vol.23 No.5

<P><B>Abstract</B></P><P>Platelets modulate asthma pathogenesis by forming the platelet‐eosinophil aggregation (PEA), which facilitates the activation of eosinophils. Platelets exhibit the purinergic receptor (P2Y12R), which responds to cysteinyl leukotriene E4 (LTE<SUB>4</SUB>). We have suggested that the combination of an antiplatelet drug (clopidogrel, [Clo]) and montelukast (Mon) would synergistically suppress asthma. BALB/c mice were intraperitoneally sensitized with ovalbumin (OVA) on days 0 and 14 and subsequently challenged on days 28‐30 and 42‐44. Mice were administered with Clo (10 mg/kg), Mon (10 mg/kg) or both drugs (Clo/Mon) orally 30 minutes before the OVA (1%) challenge on days 42‐44. Mice were assayed for airway hyper‐responsiveness (AHR) to methacholine and airway inflammation. Clopidogrel and montelukast attenuated the increased AHR; the combined treatment was more effective than a single treatment for total and eosinophil counts (all <I>P < </I>0.05). Levels of interleukin (IL)‐4, IL‐5, IL‐13, platelet factor 4, eosinophil peroxidase and LTE<SUB>4</SUB> increased in the bronchoalveolar lavage fluid of asthmatic mice, but these levels decreased in mice treated with Clo/Mon (all <I>P < </I>0.05). Goblet cell hyperplasia decreased in response to Clo/Mon. Mouse platelets and eosinophils were isolated and co‐cultured for an in vitro assay with 10 µmol/L adenosine diphosphate (ADP), LTE<SUB>4</SUB> (200 nmol/L), Mon (1 µmol/L), Clo (1 µmol/L) and Clo/Mon (1 µmol/L). Flow cytometry revealed that the increased formation of the PEA (%) was fully mediated by ADP and partly mediated by LTE<SUB>4</SUB>. Clo/Mon reduced ADP‐induced PEA formation and P‐selectin expression (<I>P < </I>0.05). In conclusion, Clo/Mon synergistically relieved asthma by inhibiting ADP‐mediated PEA formation.</P>

Unusually High‐Performing Organic Field‐Effect Transistors Based on π‐Extended Semiconducting Porphyrins

Hoang, Mai Ha,Kim, Youngmee,Kim, Minsik,Kim, Kyung Hwan,Lee, Tae Wan,Nguyen, Duc Nghia,Kim, Sung&#x2010,Jin,Lee, Kwangyeol,Lee, Suk Joong,Choi, Dong Hoon WILEY‐VCH Verlag 2012 Advanced Materials Vol.24 No.39

<P><B>Highly conjugated porphyrin derivatives, H<SUB>2</SUB>TP and ZnTP, are synthesized.</B> J‐aggregations of the H‐aggregated dimeric porphyrin pairs are clearly observed by their single crystal structures that facilitate slip‐stacked charge transport phenomenon. In particular, their SC‐FETs show the highest field‐effect mobilities of 0.85–2.90 cm<SUP>2</SUP> V<SUP>−1</SUP>s<SUP>−1</SUP>. Furthermore, the ZnTP‐based OPT displays a dramatic photoinduced current enhancement with a high photoresponsivity of 22 000 A W<SUP>−1</SUP> under a very low light intensity (5.6 m W cm<SUP>−2</SUP>).</P>

The reduced reversible phase transition field of lead–free Bi–based ceramic composites by adding nonergodic relaxor

Dinh, Thi Hinh,Tran, Vu Diem Ngoc,Nguyen, Thi Thao,Hoang, Quyen Thi Ngoc,Han, Hyoung&#x2013,Su,Lee, Jae&#x2013,Shin Elsevier 2017 CERAMICS INTERNATIONAL Vol.43 No.18

<P><B>Abstract</B></P> <P>This study investigated the microstructure, dielectric, ferroelectric, and strain properties of lead‒free 0.995Bi<SUB>1/2</SUB>(Na<SUB>0.82</SUB>K<SUB>0.18</SUB>)<SUB>1/2</SUB>TiO<SUB>3</SUB>–0.005BaZrO<SUB>3</SUB>/0.98Bi<SUB>1/2</SUB>(Na<SUB>0.78</SUB>K<SUB>0.22</SUB>)<SUB>1/2</SUB>TiO<SUB>3</SUB>–0.02LaFeO<SUB>3</SUB> (BNKT18BZ/BNKT22LF) ceramic composites. We found that the required electric field for trigging the phase transition from ergodic relaxor (ER) to ferroelectric of BNKT18BZ/BNKT22LF ceramic composites can be effectively decreased by adding BNKT18BZ as nonergodic relaxor (NER) with changes of three different stabilized regions and the highest <I>S</I> <SUB>usable</SUB> peak points as a function of the applied electric field. The highest <I>d</I> <SUB>33</SUB> <SUP>*</SUP> value of 690p.m./V was obtained at 4kV/mm for 0.3wt BNKT18BZ fraction ceramic composites. This value was higher than 575 p.m./V of BNKT22LF ceramics under an applied electric field of 5kV/mm.</P>

Preparation and characterization of silicone resin nanocomposite containing CdSe/ZnS quantum dots

Trung, Nguyen Ngoc,Luu, Quynh&#x2010,Phuong,Son, Bui Thanh,Sinh, Le Hoang,Bae, Jin&#x2010,Young Wiley Subscription Services, Inc., A Wiley Company 2012 Polymer composites Vol.33 No.10

<P><B>Abstract</B></P><P>We report the preparation of the core/shell cadmium selenide/Zinc sulfide quantum dots (CdSe/ZnS QDs)‐silicone resin nanocomposite through the solution‐mixing method, followed by thermal hydrosilylation. After dispersing QDs into Dow Corning two‐component silicone resins (OE6630A and OE6630B at 1:4 mixing ratio by weight), the resins were cured at 150°C for 1.5 h to produce QD‐silicone resin nanocomposites. The curing behavior of the silicone resins resulting from the thermal hydrosilylation was studied using differential scanning calorimetry (DSC). The properties of the QD‐silicone resin nanocomposites were investigated by ultraviolet–visible (UV–vis), fluorescence, confocal laser scanning microscopy (CLSM), atomic force microscopy (AFM), and thermogravimetric analysis (TGA) measurements. The QDs that contain trioctylamine (TOA) as the original ligand can poison the Pt catalyst in the resins and inhibit the curing process by increasing the exothermic peak temperature, at which a lower heat of hydrosilylation is observed. Incorporating a small amount of CdSe/ZnS QDs (0.1 wt%) can greatly improve the thermal stability of the silicone resins. Moreover, CdSe/ZnS QDs tend to form clusters that are relatively homogeneously distributed in a cured silicone resin, offering good optical properties of 11.2 lm W<SUP>−1</SUP> luminous efficiency and 14.6% photoluminescence conversion efficiency (PCE) in light emitting device (LED) test. POLYM. COMPOS., 2012. © 2012 Society of Plastics Engineers</P>

Thermal, dielectric, and rheological properties of aluminum nitride/liquid crystalline copoly(ester amide) composite for the application of thermal interface materials

Sinh, Le Hoang,Hong, Jeong&#x2010,Mi,Son, Bui Thanh,Trung, Nguyen Ngoc,Bae, Jin&#x2010,Young Wiley Subscription Services, Inc., A Wiley Company 2012 Polymer composites Vol.33 No.12

<P><B>Abstract</B></P><P>In this article, thermally conductive and relatively low dielectric constant polymer matrix composites of an aluminum nitride filler (AlN) and a novel liquid crystalline copoly(ester amide) (LCP) were prepared via a solution blending method in the presence of a phosphate containing dispersant. The viscosities, thermal conductivities, and dielectric properties of the prepared AlN/LCP composites were investigated as a function of AlN loading. Our experimental results demonstrated that the AIN/LCP composite with AlN concentration of 50 wt% had 2.5 times higher thermal conductivity than pure LCP (2.020 and 0.817 W/mK for composite with 50 wt% of AlN and pure LCP, respectively), but its dielectric constant remained at low level, i.e., < 9.0 at frequency of 900 Hz. In addition, viscosities of AlN/LCP pastes in the <I>N</I>‐methyl‐2‐pyrrolidinone solvent remained at acceptable levels with the high AlN loading of 50 wt%. The morphologies of the prepared composites were also investigated by scanning electron microscopy. POLYM. COMPOS., 2012. © 2012 Society of Plastics Engineers</P>

Vibration and lateral buckling optimisation of thin-walled laminated composite channel-section beams

Nguyen, Hoang X.,Lee, Jaehong,Vo, Thuc P.,Lanc, Domagoj Elsevier 2016 COMPOSITE STRUCTURES -BARKING THEN OXFORD- Vol.143 No.-

<P><B>Abstract</B></P> <P>This study presents vibration and lateral buckling optimisation of thin-walled laminated composite beams with channel sections. While flanges’ width, web’s height, and fibre orientation are simultaneously treated as design variables, the objective function involves maximising the fundamental frequency and critical buckling moment. Based on the classical beam theory, the beam element with seven degrees of freedom at each node is developed to solve the problem. Micro Genetic Algorithm (micro-GA) is then employed as an optimisation tool to obtain optimal results. A number of composite channel-section beams with different types of boundary conditions, span-to-height ratios, and lay-up schemes are investigated for the optimum design. The outcomes reveal that geometric parameters severely govern the optimal solution rather than the fibre orientation and it is considerably effective to use micro-GA compared with regular GA in term of optimal solution and convergence rate.</P>

Nutritional Supplement “Bao Khi Khang” as an Adjuvant Therapy in Acute Exacerbations of Chronic Obstructive Pulmonary Disease

Ba X. Hoang,Bo Han,Hoang X. Nguyen,Khanh T. Dang 한국식품영양과학회 2018 Journal of medicinal food Vol.21 No.10

To investigate the effectiveness of nutritional supplement Bao Khi Khang as an adjuvant therapy in acute exacerbations of chronic obstructive pulmonary disease (COPD) compared to conventional therapy alone. This was a prospective, randomized, open-label controlled trial in 60 patients presenting with acute exacerbation of COPD. Patients were randomized into two groups, the control and the experimental groups. The control group was treated with the standard therapeutic protocol (Antimicrobial+Corticosteroids+Bronchodilator+Mucolytic drugs). The experimental group was treated with Bao Khi Khang tablets combined with the standard therapeutic protocol. The treatment outcomes, including clinical and paraclinical parameters of exacerbation of COPD, were compared between the experimental group and the control group at day 15 and 30. This study was conducted according to protocol: 01.2014-HTNCKH. The results showed a significant reduction of major COPD exacerbation symptoms in experimental group. The favorable progresses in mMRC scale and COPD Assessment Test score before and after treatment, between experimental group and control group, were statistically different. Symptom control such as cough, copious sputum secretion, and bacterial infection was 90% effective (very good 50.0%, good 40.0%) in experimental group compared to 50% (very good 20%, good 30.0%) in control group. No adverse side effects were observed in experimental group. Bao Khi Khang nutritional supplement could be an effective and safe adjuvant therapy in acute exacerbations of COPD. Further interventional studies are required to confirm these findings.

Can algae-based technologies be an affordable green process for biofuel production and wastewater remediation?

Vo Hoang Nhat, P.,Ngo, H.H.,Guo, W.S.,Chang, S.W.,Nguyen, D.D.,Nguyen, P.D.,Bui, X.T.,Zhang, X.B.,Guo, J.B. Elsevier 2018 Bioresource Technology Vol.256 No.-

<P><B>Abstract</B></P> <P>Algae is a well-known organism that its characteristic is prominent for biofuel production and wastewater remediation. This critical review aims to present the applicability of algae with in-depth discussion regarding three key aspects: (i) characterization of algae for its applications; (ii) the technical approaches and their strengths and drawbacks; and (iii) future perspectives of algae-based technologies. The process optimization and combinations with other chemical and biological processes have generated efficiency, in which bio-oil yield is up to 41.1%. Through life cycle assessment, algae bio-energy achieves high energy return than fossil fuel. Thus, the algae-based technologies can reasonably be considered as green approaches. Although selling price of algae bio-oil is still high (about $2 L<SUP>−1</SUP>) compared to fossil fuel’s price of $1 L<SUP>−1</SUP>, it is expected that the algae bio-oil’s price will become acceptable in the next coming decades and potentially dominate 75% of the market.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Microalgae has more lipid content while macroalgae is rich of carbohydrate. </LI> <LI> Algae is efficient in nutrients removal. </LI> <LI> Combined processes, optimization and algae strains screening are important. </LI> <LI> Revenues in algae-based biofuel production and wastewater remediation are limited. </LI> <LI> Policy and social support for commercialization are lacking. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Effects of Trang Phuc Linh Plus-Food Supplement on Irritable Bowel Syndrome Induced by Mustard Oil

Thong T. Nguyen,Duong T. Dau,Dung C. Nguyen,Huong T.T.G. Nguyen,Phuong T.B. Ngo,Thai Q. Nguyen,Bo Han,Ba X. Hoang 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.4

Trang phuc linh plus (TPLP) is a food supplement product derived from dried extracts of herbal agents Atractylodes macrocephala, Poria cocos, Paeonia lactiflora, Phellodendron amurense, and added lactobacillus fermentum lysate (ImmuneGamma®) and 5-hydroxytryptophan. TPLP is a functional food used as adjunctive treatment for treating irritable bowel syndrome (IBS). However the biological effect and its mechanism of action in IBS have not been elucidated. In this study, we aimed to determine the pharmacological activities and mode of action of TPLP on IBS animal models. Mice were given a single administration of 5% mustard oil (MO) intracollonically. Acute colitis induction by MO resulted in later development of an IBS-like accelerated upper gastrointestinal transit in mice. Mice were treated with different does of TPLP and controls. Results showed that TPLP at the dose of 654 mg/kg/day given orally significantly decreased intestinal motility (IM) compared with the control animals. The effect was similar to Duspatalin (80 mg/kg/day) (Mebeverine Hydrochloride, an antispasmodic that helps to relieve the pain and discomfort associated with gastrointestinal spasms). Increased TPLP dose (1962 mg/kg/day) had a better effect on relief of IM than Duspatalin (80 mg/kg/day). TPLP also reduced peristalsis frequency and decreased fluid volume and electrolytes excretion in intestine tested in ex vivo models. Overall, TPLP may be an effective nutraceutical supplement for IBS.