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Lan, Truong Thi Phuong,Huy, Nguyen Duc,Luong, Nguyen Ngoc,Quang, Hoang Tan,Tan, Trinh Huu,Thu, Le Thi Anh,Huy, Nguyen Xuan,Loc, Nguyen Hoang The Korean Society of Plant Biotechnology 2019 식물생명공학회지 Vol.46 No.3
The aim of this study is to evaluate the effect of yeast extract (YE) and salicylic acid (SA) on the expression of curcuminoid-biosynthesis genes (CzDCS and CURS1-3), and accumulation of curcumin in Curcuma zedoaria cell cultures. The results showed that, in cells treated with YE or SA, the expression levels of curcuminoid genes were 1.14- to 3.64-fold higher than the control (untreated cells), in which the YE exhibited a stronger effect in comparison with SA. Curcumin accumulation also tended to be similar to gene expression, curcumin contents in YE- or SA-treated cells were 1.61- to 2.53-fold higher than the control. The SA treatment at the fifth day of culture stimulated the curcumin accumulation and expression in all four genes compared to that at the beginning. While the YE treatments gave different results, the CzCURS1 and CzCURS3 genes were expressed strongly in cells that were treated at the beginning. However, the CzDCS and CzCURS2 genes showed the opposite expression pattern, they were activated strongly in the treatments at day five of the culture. However, the content of curcumin reached its maximum value on the fifth day of culture in all investigations.
Hoang Thi Lan Anh,Le Thi Thanh Hue,Bui Nguyen Hai Linh,Trinh Thanh Trung 한국미생물학회 2023 미생물학회지 Vol.59 No.2
Consumer demand for high-quality and safe fermented meat products is increasing recently, with great attention on functional starter cultures using lactic acid bacteria (LAB). This study aimed to select autochthonous LAB strains that have beneficial effects for human health and technological properties for the production of lap xuong, a traditional fermented sausage of ethnic minorities in northwestern Vietnam. From nine lap xuong samples, 63 LAB strains were isolated and identified into 14 species belonging to 8 genera. Latilactobacillus sakei was the most common species, followed by Lactiplantibacillus plantarum, Lactococcus lactis, and Pediococcus pentosaceus. Five strains of Enterococcus faecium LM0204, Lactococcus lactis LM0302, Latilactobacillus sakei LM0405, Lactiplantibacillus plantarum LM0705, and Lactiplantibacillus plantarum LM0901 exhibited probiotic properties, including tolerance to gastrointestinal tract conditions, adhesion ability to HT-29 cell line, antagonistic activity against harmful bacteria, and antioxidant activities. The strains were further evaluated for technological and safe properties, such as gas production, growth at different temperatures and simulated fermentation and ripening stages, lactic acid production, biogenic amine production, and antibiotic susceptibility. Enterococcus faecium LM0204 and Lactococcus lactis LM0302 were excluded because of tyramine production or unable growth in simulated fermentation conditions. All together probiotic properties and better technical functionality, Latilactobacillus sakei LM0405 and Lactiplantibacillus plantarum LM0705 were proposed as starter cultures for sausage production in northwestern Vietnam. Further studies are needed to standardize the sensory quality and safety aspects of lap xuong in this area using the proposed starter cultures strains for making fermented sausage.
Nguyen Hoang Loc,Truong Thi Phuong Lan,Nguyen Duc Huy,Nguyen Ngoc Luong,Hoang Tan Quang,Trinh Huu Tan,Le Thi Anh Thu,Nguyen Xuan Huy 한국식물생명공학회 2019 JOURNAL OF PLANT BIOTECHNOLOGY Vol.46 No.3
The aim of this study is to evaluate the effect of yeast extract (YE) and salicylic acid (SA) on the expression of curcuminoid-biosynthesis genes (CzDCS and CURS1-3), and accumulation of curcumin in Curcuma zedoaria cell cultures. The results showed that, in cells treated with YE or SA, the expression levels of curcuminoid genes were 1.14- to 3.64-fold higher than the control (untreated cells), in which the YE exhibited a stronger effect in comparison with SA. Curcumin accumulation also tended to be similar to gene expression, curcumin contents in YE- or SA-treated cells were 1.61- to 2.53-fold higher than the control. The SA treatment at the fifth day of culture stimulated the curcumin accumulation and expression in all four genes compared to that at the beginning. While the YE treatments gave different results, the CzCURS1 and CzCURS3 genes were expressed strongly in cells that were treated at the beginning. However, the CzDCS and CzCURS2 genes showed the opposite expression pattern, they were activated strongly in the treatments at day five of the culture. However, the content of curcumin reached its maximum value on the fifth day of culture in all investigations.
Phuong Dinh Tam,Nguyen Luong Hoang,Hoang Lan,Pham Hung Vuong,Ta Thi Nhat Anh,Tran Quang Huy,Nguyen Thanh Thuy 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.68 No.10
In this work, we evaluated the effects of different antibody immobilization strategies on the response of a CeO2-nanowires (NWs)-based immunosensor for V ibrio cholerae O1 detection. Accordingly, the changes in the electron-transfer resistance (Ret) from before to after cells bind to an antibody-modified electrode prepared by using three different methods of antibody immobilization were determined. The values were 16.2%, 8.3%, and 6.65% for the method that utilized protein A, antibodies activated by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/Nhydroxysuccinimide (NHS), and absorption, respectively. Cyclic voltammetry confirmed that the change in the current was highest for the immunosensors prepared using protein A (11%), followed by those prepared with EDC/NHS-activated antibodies (9%), and finally, those prepared through absorption (7.5%). The order of the antibody immobilization strategies in terms of resulting immunosensor detection limit and sensitivity was as follows order: absorption (3.2 × 103 CFU/mL; 45.1 /CFU·mL−1) < EDC/NHS-activated antibody (1.0 × 103 CFU/mL; 50.6 /CFU·mL−1) < protein A (1.0 × 102 CFU/mL; 65.8 /CFU·mL−1). Thus, we confirmed that the protein A - mediated method showed significantly high cell binding efficiencies compared to the random immobilization method.
Dang, Diem-Hong,Luyen, Hai-Quoc,Hien, Hoang Thi Minh,Thu, Ngo Hoai,Anh, Hoang Lan The Korean Society for Marine Biotechnology 2007 한국해양바이오학회지 Vol.2 No.1
For the first time in Vietnam, morphological and molecular studies of a species belonging to Bacillariophyceae collected in Northern coast of Vietnam are presented. Observations with microscope showed that this species belong to genus: Pseudo-nitzschia and seem like P. pungens. Sequence data from the partial 18S small subunit ribosomal RNA gene (18S rDNA) and the internal transcribed spacer 1 - 5.8S - internal transcribed 2 have been used to determine clearly and generate a phylogenetic framework of the obtained sequences to previously reported sequences in GenBank. These results allowed us to highlight described species of Bacillariophyceae in Northern coast of Vietnam. Furthermore, accumulation of molecular study would be helpful for the identification of scientific name of harmful algal species and further taxonomic studies in Vietnam.
Duy Le Nguyen,Hieu Le-Trung Hoang,Vu Ngoc-Anh Ho,Toan Duong Pham,Nam Thanh Nguyen,Van Thi-Thu Tran,Tuong Manh Ho,Lan Ngoc Vuong The Korean Society for Reproductive Medicine 2024 Clinical and Experimental Reproductive Medicine Vol.51 No.1
Objective: The purpose of this study was to identify factors associated with twin pregnancy following day 3 double embryo transfer (DET). Methods: This retrospective cohort study incorporated data from 16,972 day 3 DET cycles. The participants were women aged between 18 and 45 years who underwent in vitro fertilization with intracytoplasmic sperm injection (IVF/ICSI) at My Duc Assisted Reproduction Technique Unit (IVFMD), My Duc Hospital, located in Ho Chi Minh City, Vietnam. Results: Of the 16,972 day 3 DET cycles investigated, 8,812 (51.9%) resulted in pregnancy. Of these, 6,108 cycles led to clinical pregnancy, with 1,543 (25.3% of clinical pregnancies) being twin pregnancies. Factors associated with twin pregnancy included age under 35 years (odds ratio [OR], 1.5; 95% confidence interval [CI], 1.32 to 1.71; p<0.001) and cycles involving the transfer of at least one grade I embryo. Relative to the transfer of two grade III embryos, the risk of twin pregnancy was significantly elevated following the transfer of two grade I embryos (OR, 1.40; 95% CI, 1.16 to 1.69; p<0.001) or a combination of one grade I and one grade II embryo (OR, 1.27; 95% CI, 1.05 to 1.55; p=0.001). Conclusion: By analyzing a large number of IVF/ICSI cycles, we identified several predictors of twin pregnancy. These findings can assist medical professionals in tailoring treatment strategies for couples with infertility.