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Hino, T.,Mukunoki, H.,Imanishi, K.,Miyazaki, K. Asian Australasian Association of Animal Productio 1992 Animal Bioscience Vol.5 No.3
Ethanol was utilized by mixed rumen microbes, but addition of pentachlorophenol (25 mg/l), a methanogen inhibitor, suppressed the utilization of ethanol. Carbon monoxide (50% of the gas phase), a hydrogenase inhibitor, more strongly suppressed the utilization of ethanol, propanol, and butanol. These results suggest that the major ethanol utilizers are $H_2$ producers. Ethanol utilization was depressed at low pH (below 6.0). Since methanogens were shown to be relatively resistant to low pH, it appears that ethanol utilizers are particularly sensitive to low pH. Ruminococcus albus and R. flavefaciens in mono-culture produced ethanol from carbohydrate (glucose and cellobiose), even when a high level (170 mM) of ethanol was present. Ethanol was not utilized even in the absence of carbohydrate, but the co-culture of these bacteria with methanogens resulted in the utilization of ethanol, i.e., when $H_2$ was rapidly converted to $CH_4$, R. albus and R. flavefaciens utilized ethanol. These results suggest that ethanol is utilized when the electrons liberated by the oxidation of ethanol are rapidly removed, and ready electron disposal in ethanol-utilizing, $H_2$-producing bacteria is accomplished by the interspecies transfer of $H_2$.
Optimum PVD installation depth for two-way drainage deposit
Chai, J.C.,Miura, N.,Kirekawa, T.,Hino, T. Techno-Press 2009 Geomechanics & engineering Vol.1 No.3
For a two-way drainage deposit under a surcharge load, it is possible to leave a layer adjacent to the bottom drainage boundary without prefabricated vertical drain (PVD) improvement and achieve approximately the same degree of consolidation as a fully penetrated case. This depth is designated as an optimum PVD installation depth. Further, for a two-way drainage deposit under vacuum pressure, if the PVDs are fully penetrated through the deposit, the vacuum pressure will leak through the bottom drainage boundary. In this case, the PVDs have to be partially penetrated, and there is an optimum installation depth. The equations for calculating these optimum installation depths are presented, and the usefulness of the equations is studied by using finite element analysis as well as laboratory model test results.