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염행철 ( Heng Cherl Yom ),조성연 ( Song Yon Cho ) 한국청소년복지학회 2007 청소년복지연구 Vol.9 No.2
This study was to investigate the effect of the ecological variables on the stress of Korean adolescents. The ecological variables in this study were self-esteem for individuals, relationship with teachers and friends for school environments, city size(big city, small city, and county) for the community environment. The data in this study were used from the Korea Youth Panel Survey for 3 years from 2003. The participants were 3,388 students from the second year and 3,107 from the third year in middle schools, and 3,033 from the first year in high schools. Cheju-Do was excluded in the survey. The data were analysed by SPSS 12.0 version for ANCOVA, Pearson`s partial correlation, stepwise multiple regression, Tukey test for post hoc test, and factor analysis. The results were as follows; firstly, there were significant differences by gender in all school year groups and significant difference by city size only from the third year in middle school. Secondly, there were significant negative correlations with `adolescents` self-esteem` and `relationship with parents` on their stress in all school year groups of every city groups. Also, there were various significant negative correlations with `relationship with friends` and `relationship with teachers` on their every stress according to all school grades and city size. Finally, the ecological variables were explained by 16~29% on their stress, and self-esteem was the most important variable.
Lentivirus-mediated Gene Transfer to Bovine Embryos
Young Mi Kim,Mo Sun Kwon,Bon Chul Koo,Teoan Kim,Heng-Cherl Yom,Dae Hwan Ko 한국동물생명공학회(구 한국동물번식학회) 2008 Reproductive & developmental biology Vol.32 No.1
Pronuclear DNA microinjection has been the most universal method in transgenic animal production but its success rate of transgenesis in mammals are extremely low. To address this long-standing problem, we used retrovirus- and lentivirus-based vectors carrying the enhanced green fluorescent protein (EGFP) gene under the control of ubiquitously active cytomegalovirus (CMV) promoter to deliver transgenes to bovine embryos. The rate of transgenesis was evaluated by counting EGFP positive blastocysts after injection of concentrated virus stock into the perivitelline space of the bovine oocytes in metaphase II. Among two different types of lentivirus vectors derived from FIV (feline immunodeficiency virus) and HIV (human immunodeficiency virus), the former scored the higher gene transfer efficiency; almost 100% of the blastocysts developed from the oocytes infected with FIV-based vector were EGFP positive. As for the vectors derived Com HIV lentivirus, the transgenesis rate of the blastocysts was reduced to 39%.
Kim, Teoan,Lee, Young Man,Lee, Hoon Taek,Heo, Young Tae,Yom, Heng-Cherl,Kwon, Mo Sun,Koo, Bon Chul,Whang, Key,Roh, Kwang Soo Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.2
Despite the high potency of the retrovirus vector system in gene transfer, one of the main drawbacks of has been difficulty in preparing highly concentrated virus stock. Numerous efforts to boost the virus titer have ended in unsatisfactory results mainly due to fragile property of retrovirus envelope protein. In this study, to overcome this problem, we constructed our own retrovirus vector system producing vector viruses encapsulated with VSV-G (vesicular stomatitis virus G glycoprotein). Concentration process of the virus stock by ultracentrifuge did not sacrifice the virus infectivity, resulting in more than 108 to 109 CFU (colony forming unit) per ml on most of the target cell lines tested. Application of this high-titer retrovirus vector system was tested on chicken embryos. Injection of virus stock beneath the blastoderms of pre-incubated fertilized eggs resulted in chick embryos expressing E. coli LacZ gene with 100% efficiency. Therefore, our results suggest that it is possible to transfer the foreign gene into chicken embryo using our high-titer retrovirus vector.
Lentivirus-mediated Gene Transfer to Bovine Embryos
Kim, Young-Mi,Kwon, Mo-Sun,Koo, Bon-Chul,Kim, Teo-An,Yom, Heng-Cherl,Ko, Dae-Hwan The Korean Society of Animal Reproduction 2008 Reproductive & developmental biology Vol.32 No.1
Pronuclear DNA microinjection has been the most universal method in transgenic animal production but its success rate of transgenesis in mammals are extremely low. To address this long-standing problem, we used retrovirus- and lentivirus-based vectors carrying the enhanced green fluorescent protein (EGFP) gene under the control of ubiquitously active cytomegalovirus (CMV) promoter to deliver transgenes to bovine embryos. The rate of transgenesis was evaluated by counting EGFP positive blastocysts after injection of concentrated virus stock into the perivitelline space of the bovine oocytes in metaphase II. Among two different types of lentivirus vectors derived from FIV (feline immunodeficiency virus) and HIV (human immunodeficiency virus), the former scored the higher gene transfer efficiency; almost 100% of the blastocysts developed from the oocytes infected with FIV-based vector were EGFP positive. As for the vectors derived Com HIV lentivirus, the transgenesis rate of the blastocysts was reduced to 39%.