Remedial efficiency study of microbial agents onto contaminated bay sediment of the southeast coast of Korea

Md. Mahabub Alam,Md. Niamul Haque,Sung-hyun Kwon 한국해양환경·에너지학회 2015 한국해양환경공학회 학술대회논문집 Vol.2015 No.5

The purposes of this study are to measure environmental condition of the contaminated area and to evaluate remedial efficiency of microbial agents in the contaminated sediment. For this purpose sediments were collected from two different locations, dangdong and danghangpho in the wonmun bay, southeast coast of Korea. Three microbial agents such as Bacillus Bacteria (A), mixture of montmorillonite (B) and biogranula (C) were applied onto contaminated bay sediment to activate microbial population. Geochemical parameters such as chemical oxygen demand (CODMn), total phosphorus (T-P), total nitrogen (T-N), and acid volatile sulfide (AVS) were measured to know environmental condition of the contaminated area. The remedial efficiency of microbial agents was calculated with compared to control sediment within 28 days. The bacterial growth of microbial agents was observed by the total viable count (TVC) method. Those microbial agents were found to reduce chemical oxygen demand (CODMn), total phosphorus (T-P), total nitrogen (T-N), and acid volatile sulfide (AVS) value. Pollutant removal efficiency order of microbial agents were found as microbial agent A > microbial agent B > microbial agent C. These results showed that microbial agent A can reduce chemical oxygen demand (CODMn), total phosphorus (T-P), total nitrogen (T-N), and acid volatile sulfide (AVS) value in the contaminated sediments. Microbial agent A is available in market, easy to spray and comparatively low cost. Therefore the Bacillus Bacteria is a promising agent for remediation of contaminated sediments.

Acute and Chronic Effects of Nanoplastics on the Water Flea Moina macrocopa

Haque Md. Niamul,Kim Jaehee,Rhee Jae-Sung (사)한국해양생명과학회 2023 한국해양생명과학회지 Vol.8 No.1

Here, upon acute (96 h) and chronic (14 days) exposure, ingestion of polystyrene NPs (100 nm) and physiological, biochemical, and cholinergic modulations were analyzed in the water flea Moina macrocopa exposed to different concentrations (0.001, 0.01, 0.1, 1, 5, 10, 50, 100, and 500 μg l-1). Exposed NPs were observed in the internal organs (e.g., digestive tract and foregut) of the water flea. Chronic exposure to the relatively high concentrations resulted in significant decreases in survival, body length, and the total number of molts, whereas reproduction parameter was not affected. Significant increase in oxidative stress biomarker (malondialdehyde) and decrease in the intracellular content of endogenous antioxidant (glutathione) and enzymatic activity of antioxidant enzymes (glutathione peroxidase, glutathione reductase, catalase, superoxide dismutase, and glutathione S-transferase) were detected in response to relatively high concentrations of NPs. Transcriptional expression of the hsp70 gene was increased in response to relatively high concentrations of NPs, whereas acetylcholinesterase activity was lowered by the same concentrations of NPs. Taken together, NPs exposure would be a significant modulator on physiological and biochemical metabolism of water flea.

Exposure to Sublethal Concentrations of Copper Pyrithione Reduces Cholinergic Activity and Induces Oxidative Stress in a Marine Polychaete

Haque Md. Niamul,이재성 (사)한국해양생명과학회 2022 한국해양생명과학회지 Vol.7 No.2

Despite concerns about the significant toxicity of copper pyrithione (CuPT) at environmental concentrations, effects of CuPT on benthic organisms have received little attention. Here, we analyzed the detrimental effects of CuPT at sublethal concentrations (1/50, 1/20, and 1/10 of the 96 h-LC50 value) for 14 days in the marine polychaete Perinereis aibuhitensis. Reduced burrowing activity and significantly decreased the acetylcholinesterase activity in response to relatively high concentrations of CuPT were identified as CuPT-triggered cholinergic inhibition. The lipid peroxidation marker, malondialdehyde levels were dose- dependently increased, whereas intracellular glutathione was depleted by relatively high concentrations. In the CuPT-treated polychaete, significant fluctuations in the enzymatic activities of the antioxidant defense system (catalase, superoxide dismutase, glutathione reductase, and glutathione peroxidase) were observed with significantly modulated glutathione S-transferase activity. These results indicate that even sublethal levels of CuPT would have detrimental effects on the health status of the marine polychaete.

Dose- and age-specific antioxidant responses of the mysid crustacean <i>Neomysis awatschensis</i> to metal exposure

Haque, Md. Niamul,Lee, Do-Hee,Kim, Bo-Mi,Nam, Sang-Eun,Rhee, Jae-Sung Elsevier 2018 Aquatic toxicology Vol.201 No.-

<P><B>Abstract</B></P> <P>Waterborne metals can adversely affect an organism’s innate defenses through oxidative stress. In the present study, the marine mysid <I>Neomysis awatchensis</I> was exposed to sublethal concentrations (1/10 and 1/5 of the median LC50s) of As, Cd, Cu, Pb, and Zn for 48 or 96 h at the juvenile and adult developmental stages, and the dose- and age-specific antioxidant defense system responses were characterized. Metal accumulation and modulation of four key antioxidant biomarkers, malondialdehyde (MDA), glutathione (GSH), superoxide dismutase, and catalase, were measured in juvenile and adult mysids. Based on LC50, level of metal toxicity was dependent on metal concentration, developmental stage, and the exposure duration. Intracellular MDA content was increased in the As-, Cu-, Pb-, and Zn-exposed juvenile mysids after exposure for 48 and/or 96 h, while increases in MDA content were observed in adult mysids following Cu and Zn exposure. Interestingly, GSH content was differentially modulated, where intracellular GSH levels decreased in juvenile mysids following Cd, Cu, Pb, and Zn exposure, but significantly increased in metal-exposed adult mysids, except for Pb exposure. The catalase and superoxide dismutase activities displayed similar stage-specific increases or decreases as also observed for the different GSH levels, suggesting that the susceptibility to and defense against metal-induced oxidative stress differed based on stage. Modulations in MDA and GSH content and enzymatic activity of the antioxidant defense system indicate that mysid antioxidant defense system factors are intimately connected during control of oxidative imbalances with different capacities at different developmental stages.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Evidence on the age-specific bioconcentration of five metals in the marine mysid was obtained. </LI> <LI> Age-specific relationship between metal dynamics and ecotoxicity between ages was observed. </LI> <LI> Oxidative stress was induced by metals and caused distinctive age-specific antioxidant response. </LI> </UL> </P>

Assessing the hydrogen peroxide effect along with sodium hypochlorite against marine blue mussels aimed at antifouling usage

Md. Niamul Haque,Sunghyun Kwon 대한환경공학회 2017 Environmental Engineering Research Vol.22 No.1

Chlorination has been the most common antifouling method, but alternatives are under searching. In this article, we report how the hydrogen peroxide could enhance the effect of chlorination to prevent fouling by inhibiting larvae settlement and abatement of mussel colonization or by extinct of them; through marine mussel Mytilus edulis. The addition of hydrogen peroxide shows synergic effect on the veliger larvae (up to 19 folds) and effectively reduces required time of mussel mortality by 8-22%. For resolution of micro- and macro-fouling caused by the marine mussel, as well as diminishing of time and conventional chlorine dose could be important factor in favour of environment and economics.

Physiological effects of biocide on marine bivalve blue mussels in context prevent macrofouling

Md. Niamul Haque,권성현 한국생태학회 2016 Journal of Ecology and Environment Vol.40 No.3

Background: Mussels are stubborn organisms attached to solid substrata by means of byssus threads. The abundance of marine mussel Mytilus edulis in marine facilities like power stations was reason to select among fouling animals. Methods: Mortality patterns as well as physiological behavior (oxygen consumption, foot activity, and byssus thread production) of two different size groups (14- and 25-mm shell length) of M. edulis were studied at different hydrogen peroxide concentrations (1–4 mg l−1). Results: Studied mussels showed progressive reduction in physiological activities as the hydrogen peroxide concentration increased. Mussel mortality was tested in 30 days exposure, and 14 mm mussels reached the highest percentage of 90% while 25 mm mussels reached 81%. Produced data was echoed by Chick-Watson model extracted equation. Conclusions: This study points that, while it could affect the mussel mortality moderately in its low concentrations, hydrogen peroxide has a strong influence on mussels’ physiological activities related to colonization. Therefore, hydrogen peroxide can be an alternative for preventing mussel colonization on facilities of marine environment.

Enhanced natural bioremediation using a assessment and study of potential controlling method

Md. Niamul Haque,Sung-Hyun Kwon(권성현) 한국해양환경·에너지학회 2015 한국해양환경공학회 학술대회논문집 Vol.2015 No.11

Oyster and ark shell farm sediment pollution assessment and study of potential controlling method

Md. Niamul Haque,Hwan-Jin Bae,Md. Mahabub Alam,Daechul Cho,Sunghyun Kwon 한국해양환경·에너지학회 2015 한국해양환경공학회 학술대회논문집 Vol.2015 No.5

Volatile organic compounds (VOCs) adsorption and thermal insulation study of oyster shell powder

Md. Niamul Haque,Junghyun Kwon,Suwan Ahn,Jongsoo Lee,Sunghyun Kwon 한국해양환경·에너지학회 2015 한국해양환경공학회 학술대회논문집 Vol.2015 No.5

Oyster shell waste has been a serious hazard in many fishery areas in Korea. Oyster waste has also been said to be a promising adsorbent for reducing popular environmental contaminants such as volatile organic compounds (VOC) and thermal conductivity incorporated with building materials also an important characteristics. We investigated those properties in this research. Thermal conductivity measured by thermograph and the concentrations of the contaminants determined by HPLC or FID method in gas chromatography. Based on Fourier’s 2<SUP>nd</SUP> law of heat conduction we found that conductionrelated coefficients and parameters could greatly influence on the thermal profile of oyster shell. 180 minute adsorption study results revealed that the initial concentrations did not seem to make any difference in adsorption performance but higher oyster content strongly lead to higher VOC adsorption. This research was supported by the Fund of Research Promotion Program(2014-03-011), Gyeongsang National University, 2014.

Physiological effects of biocide on marine bivalve blue mussels in context prevent macrofouling

Haque, Md Niamul,Kwon, Sung-Hyun The Ecological Society of Korea 2016 Journal of Ecology and Environment Vol.40 No.3

Background: Mussels are stubborn organisms attached to solid substrata by means of byssus threads. The abundance of marine mussel Mytilus edulis in marine facilities like power stations was reason to select among fouling animals. Methods: Mortality patterns as well as physiological behavior (oxygen consumption, foot activity, and byssus thread production) of two different size groups (14- and 25-mm shell length) of M. edulis were studied at different hydrogen peroxide concentrations ($1-4mg\;l^{-1}$). Results: Studied mussels showed progressive reduction in physiological activities as the hydrogen peroxide concentration increased. Mussel mortality was tested in 30 days exposure, and 14 mm mussels reached the highest percentage of 90% while 25 mm mussels reached 81%. Produced data was echoed by Chick-Watson model extracted equation. Conclusions: This study points that, while it could affect the mussel mortality moderately in its low concentrations, hydrogen peroxide has a strong influence on mussels' physiological activities related to colonization. Therefore, hydrogen peroxide can be an alternative for preventing mussel colonization on facilities of marine environment.