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Effective Multiplication of Somatic Embryos Using Suspension Culture and Regeneration in Soybean
Kim, Young Jin,Park, Tae Il,Kim, Hyun Soon,Suh, Sug Kee,Kim, Hag Sin,Yun, Song Joong The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.2
The use of liquid-medium-based procedure relative to the solid media led to a 4.5-fold increase in the number of cotyledon-stage embryos. The most efficient system for multiplication and regeneration of somatic embryos was CP6 procedure with the media MSD40/MSD20/MSM6AC/FNL0S3S3GM. However, the rate of regeneration was lower. About 71% of the embryos with dicotyledon were continued to develop the roots after desiccation treatment and 92% of the germinated embryos produced shoots in 10 days. Of the four morphologically different types of embryos, dicotyledonous ones showed a high frequency of conversion, while only a few with fused and horn type cotyledon developed shoots. Mature somatic embryos were desiccated in empty petri dishes for 12-72 h. Embryo survival rate was the highest after 12 h of desiccation, but maximal germination was observed at 24 h. After desiccation, they were placed on MS medium without growth regulators for germination. Germinating embryos were transferred to small pots with vermiculite for plant regeneration. The etiolating the plants during the growth was resolved to add 1% activated charcoal on hormone-free MS medium.
The Factors on Somatic Embryogenesis of Soybean [Glycine max. (L.) Merrill]
Kim, Kyong-Ho,Kim, Hag-Sin,Oh, Young-Jin,Suh, Sug-Kee,Kim, Tae-Soo,Park, Ho-Kee,Park, Moon-Soo,Kim, Seok-Dong,Yeo, Up-Dong The Korean Society of Plant Biotechnology 2000 Plant molecular biology and biotechnology research Vol.2 No.3
To enhance in vitro plantlet regeneration efficiency of soybean through embryogenesis, the culture conditions such as material part and size of immature seed, 2,4-D, pH and solidifying agents for somatic embryogenesis were investigated. Somatic embryogenesis was induced from the immature embryo, immature cotyledon and embryonic axis explants of the immature seed on MS medium supplemented with 2.0 mg/L 2,4-D. The highest rate (up to 22.9%) of somatic embryogenesis was obtained from the immature cotyledon, following embryonic axis and the immature embryo. The rate varied with the developmental stages of seed. The maximum rate (25.4%) of embryogenesis was obtained from 3-4 mm length of the seed (after 25 days of flowering). The optimum concentration of 2,4-D for embryogenesis was 10 mg/L. The optimum pH was at 5.8 and solidifying agent for medium was better with 0.4% gelrite than with agar. For rapid multiplication of shoot tips from the germinating somatic embryos, they were cultured on MS medium containing 2 mg/L indole-3-butyyic acid (IBA) and 1 mg/L 6-benzyladenine (BA). After then somatic embryos with one and three cotyledons were transferred to the growth regulator free medium. The medium exhibited the higher rate (ca. 50%) of development than the multiplication medium.
Effective Acclimation System for in Vitro Regenerated Plant lets of Soybean
Kim, Young Jin,Park, Tae Il,Kim, Hyun Soon,Suh, Sug Kee,Kim, Hag Sin,Yun, Song Joong The Korean Society of Plant Biotechnology 2004 Plant molecular biology and biotechnology research Vol.6 No.2
To establish an efficient acclimation system for regenerated plantlets of soybean, we used various media with hydroponic nutrient solutions before regenerants were transplanted into soil. The hydroponic nutrient solution was essential for the survival of the plantlets. The vermiculite with nutrient solution at pH 5.5 was found to be the best medium with 97-100% survival rate and better growth of regenerants plantlets. Regeneraed grew best in the following order of solutions: Yoshida solution, modified Yoshida solution, SoyI, Soy II, and MS medium. However, Soy I solution (EC 2.9 mS/cm), developed by the Honam Agricultural Research Institute proved to be the most effective for acclimation in terms of the time required for vigorous growth and economical use of chemicals.
Factors on Somatic Embryogenesis and Organogenesis of Soybean (Glycine max)
Kim, Kyong-Ho,Oh, Young-Jin,Suh, Sug-Kee,Kim, Hag-Sin,Kim, Tae-Soo,Lee, Mi-Ja,Park, Ho-Ki,Suh, Duck-Yong,Yeo, Up-Dong 한국콩연구회 2002 韓國콩硏究會誌 Vol.19 No.1
To enhance in vitro plantlet regeneration efficiency of soybean through embryogenesis, the culture conditions such as meterial part and size of immature seed, 2,4-D pH and solidifying agents for somatic embryogenesis were investigated. Somatic embryogenesis was induced from the immature embryo, immature cotyledon, and embryonic axis explants of the immature seed on MS medium supplemented with 2.0 mg/L 2,4-D. The highest rate (up tp 23%) of somatic embryogenesis was obtained from the immature cotyledon, followed by embryonic axis and immature embryo. The rate varied with the developmental stages of seed. The maximum rate (25%) of embryogenesis was obtained from 3-4 mm length of the seed after 25 days of flowering. The optimum concentration of 2,4-D for embryogenesis was 10mg/l. The optimum pH was at 5.8 and solidifying agent for medium was better with 0.4% gelrite than with agar. For rapid multiplication of shoot tip from germinating somatic embryos, they were cultured on MS medium containing 2 mg/L IBA and 1 mg/L BAP, After then somatic embryos with one and three cotyledons were transferred to the plant growth regulator free medium. The medium exhibited the higher rate (50%) of development than the multiplication medium. To select the section with shoot formation ability, the calli and shoot formation from three sections of 5-days-seedings of soybean were induced on MS medium supplemented with 1.0 mg/L BAP, 3% sucrose, and 0.3% gelrite for one month. The first leaf section exhibited the highest shoot formation rate (51%), followed the hypocotyl section and the cotyledon section. The shoot formation rates and shoot number of the four excised sections of the first leaf were aldo investigated on the same medium. A half of the first leaf explant and the third of the first leaf explant had higher shoot formation rates (76-80%) and number (3-4/ explants) than those in other two explants. Effects of cytokinins on shoot formation were determined, using the half of the first leaf explants. Zeatin (1.0mg/L) exhibited the highest in shoot formation rate (94%) and combination (1: 1, v/v) of zeatin (1.0mg/L) and IAA (1.0 mg/L) eshibited the highest in shoot formation rate (96%) and numbers (16/explant), twice more than zeatin alone (1.0 mg/L). The shoot cuttings were transferred and cultivated on the rooting media supplemented with only auxin, IBA at variois concentrations. The highest root formation (8/shoot) was achieved on the medium supplementes with 1.5 mg/L. After 4 weeks of cultivation, the plantlets with an extensive root system were transplanted in pots with a soil mixture of vermiculite and fine sand. Transferred to field, about 75% of the plantlets survived.
경기장 부대서비스시설 만족이 이벤트이미지와 재관람에 미치는 영향
김학신(Kim Hag Sin) 한국사회체육학회 2003 한국사회체육학회지 Vol.19 No.1
This study was intended to investigate the effect of spectator`s event image and intention to revisit in an attempt to establish the plan for effective operational management of the professional baseball field. As a result, the following conclusion was drawn: First it was found that as for the service facility incidental to the stadium male spectators showed a higher level of satisfaction with factors such as F&B facilities and the bathroom than female counterparts. Seen in terms of age, the group of spectator belonging to the 10s and more than the 40s of age showed the higher level of satisfaction than the other age groups. Seen in terms of occupation, spectators employed in self-employed business, business/specialized technical job, and professional/freelance showed a relatively higher level of satisfaction compared to the other occupational groups. And seen in terms of monthly average income, the club-run concessions and F&B concessions showed the relatively higher level of satisfaction compared to the other income brackets. Especially, it was found that satisfaction with the service facility incidental to the stadium had the significant influence on the event image included the club-run concession, F&B facility, seating, bathroom, parking lots and the like. And it was found that F&B facilities, seating, bathroom and club-run concessions had a significant effect on spectators` intention to revisit the event.
동시작업기를 이용한 밀 재배시 노력절감 및 농업적 형질의 차이
김학신 ( Hag Sin Kim ),김경훈 ( Kyeong Hoon Kim ),강천식 ( Chon Sik Kang ),신상현 ( Sang Hyun Shin ),정영근 ( Young Kun Jeong ),이춘기 ( Choon Ki Lee ),김경호 ( Kyung Ho Kim ),박광근 ( Kwang Geun Park ),박기훈 ( Ki Hun Park ) 전북대학교 농업과학기술연구소 2014 농업생명과학연구 Vol.45 No.1
밀 재배의 생력화와 생산비 절감을 위한 밀 파종방법을 산파 후 배수골 형성과 동시에 복토하는 광산파와 파종단계의 파종, 시비, 배토, 배수골 형성을 일괄작업이 가능한 동시작업기를 활용한 줄뿌림파종에 따른 밀의 생육 및 수량성을 검토한 시험 결과를 요약하면 다음과 같다. 1. 밀 파종단계의 작업시간은 개별 작업인 기계 산파는 17.0시간 ㏊-1이 소요되나 동시작업기 이용 줄뿌림은 8.7시간으로 49%의 노력이 절감되었다. 2. 밀 입모율은 파종방법이나 파종량 간에 차이가 없었으며, 2010년 입모율이 71~72%로 낮았던 것은 파종에서 출현까지 토양수분이 부족하였기 때문이었으며, 농업적 형질은 기계 산파보다 동시파종기 줄뿌림에서 다소 좋았으나 간장은 오히려 작은 경향을 보였다. 3. 파종방법간 간장은 산파는 68.8cm로 줄뿌림보다 9.3cm가 컸으며, 수장, 수당 입수는 줄뿌림이 많았고 파종량 간에는 산파는 180kg ha-1에서, 줄뿌림은 2000kg ha-1에서 길거나 많았다. m2당 수수와 천립중은 파종방법간에 차이가 없었으며 200kg ha-1 파종에서 많은 경향을 보였다. 4. 수량성은 파종방법간에는 산파(3.79톤 ha-1)보다는 줄뿌림(3.94톤 ha-1)이 4%가 증수되었고, 파종량간에는 기계 산파는 ha당 150kg 파종에 비하여 기계 산파 180kg는 12%, 동시작업기 줄뿌림 180kg는 5%, 동시작업기 줄뿌림 200kg 에서 22%가 증수되었다. 5. 이상의 결과를 요약하면 생산비 절감을 위한 일괄작업기를 이용 밀 재배시 적정 파종량은 ha당 180~200kg 수준이며, 최소 작업면적은 1.0ha 이상으로 판단되었다. This study was performed to evaluate the effects of seeding method using ridger to growth and grain yield increasement in wheat. This study was conducted by the National institute of crop science, Iksan, Korea for two years (2010 to 2011). Two types of seeding methods, Broadcast seeding(BS) and drill seeding(DS), were used to compare in three different seeding rates. Culm length of DS methods was shorter than BS methods. Agricultural characteristics were improved by DS methods. The average of grain yield by DS and BS methods were 3.79ton/㏊ and 3.94ton/㏊, respectively. According to two different seeding rate, 180kg/ha and 200 kg/ha, the grain yields were 3.98 tons in the DS methods. This means that yield were increased 5% and 22% compared with BS methods, respectively. Using of the ridge, labor time was reduced about 8.3 hours in DS methods. Consequently, using ridger have high efficiency under the optimum condition that was 180- 200kg seeding rates at least of 1.0 ha.
고농도 산소로 유발한 흰쥐의 급성폐손상모델에서 폐포대식세포 아형군의 분포와 형태 변화
신윤 ( Sin Yun ),이상학 ( Lee Sang Hag ),윤형규 ( Yun Hyeong Gyu ),이숙영 ( Lee Sug Yeong ),김석찬 ( Kim Seog Chan ),권순석 ( Kwon Sun Seog ),김영균 ( Kim Yeong Gyun ),김관형 ( Kim Gwan Hyeong ),문화식 ( Mun Hwa Sig ),송정섭 ( So 대한결핵 및 호흡기학회 2000 Tuberculosis and Respiratory Diseases Vol.48 No.4
레저스포츠 제품에 대한 가치지각이 재구매의도와 충성도에 미치는 영향
김학신(Kim Hag Sin),박용범(Park Yong Beom) 한국사회체육학회 2003 한국사회체육학회지 Vol.20 No.1
This study was intended to identify the effect of perceived value on repurchase and loyalty for consumers experienced in purchasing and using skiing articles, golfing articles, fitness articles and the like. As a result, the following findings were obtained: First, it was found that consumers with the high level of perceived value showed the very significant correlation between the perceived value on the product and its repurchase. Second, it was found that consumers with the high level of perceived value showed the very significant correlation between the perceived value on the product and its loyalty. Second, it was found that consumers`s higher level of perceived value had a significant effect on the intention of repurchase. Fourth, it was found that consumers`s higher level of perceived value on the product had a significant effect on loyalty. It can be seen that consumers` perceived value on the sporting products reflected the corporate image, recognition, reliability and the like in all-inclusive sense. For this reason, the firm which will engage in marketing in the target market needs to continue an reliable access to it with socially good consciousness as well as the approach to enhance the value of the product.