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The Effects of rhBMP-2 Injection at Distraction Osteogenesis of Rats` Tibia
( Hae Ryong Song ),( Sung Eun Kim ),( Hyo Geun Kim ),( Young Pil Yun ),( Ji Hoon Bae ),( Hak Jun Kim ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.2
Distraction osteogenesis is the popular method of bone-lengthening procedure. Delayed consolidation of bone after distraction osteogenesis makes complications related to external fixator and refractures after removal of external frame. We hypothesized that only rhBMP-2(recombinant human bone morphogenetic protein-2) could accelerate the long bone healing. 18 Sprague-Dawley rats were divided into two groups after 5 mm lengthening by distraction osteogenesis. The control group was none-injected group and the experimental group was injected 0.5 cc of 0.05 mg/ml rhBMP-2. We evaluated the 6 samples respectively at different time point (2, 4, 8 weeks) using simple radiographs, micro-CT and histological stains. According to the simple radiographs, bone consolidation of percentage pixel count was higher in the experimental group at 2, 4, 8 week, and statistically significant higher than the control group at 8 weeks (p < 0.05). Percentage bone Volume, trabecular thickness in the experimental group was higher than the control group by evaluation with micro-CT. According to the histological examinations, neovascularization and new stromal cells were observed in the control group at 2 week- speciemen but intramembranous ossification was seen at 2 week- specimens in the experimental group. Intramembranous and enchondral ossification was seen in the experimental group but not seen in the control group at the 4 week-specimens. Trabeculae thickness in the experimental group was thicker than the control group at 8-week specimens. Only local injection of rhBMP- 2 at the distraction site could accelerate the bone healing during distraction osteogenesis.
Molecular Cloning and Characterization of a Bile Salt Hydrolase from Lactobacillus acidophilus PF01
( Hae Keun Oh ),( Ji Yoon Lee ),( Soo Jin Lim ),( Min Jeong Kim ),( Geun Bae Kim ),( Jung Hoan Kim ),( Soon Kwang Hong ),( Dae Kyung Kang ) 한국미생물 · 생명공학회 2008 Journal of microbiology and biotechnology Vol.18 No.3
Helical Aromatic Foldamers Functioning as a Fluorescence Turn-on Probe for Anions
Jeon, Hae-Geun,Jang, Han Bit,Kang, Philjae,Choi, Ye Rin,Kim, Junyoung,Lee, Ji Hyun,Choi, Moon-Gun,Jeong, Kyu-Sung American Chemical Society 2016 ORGANIC LETTERS Vol.18 No.17
<P>Indolocarbazole-pyridine hybrid foldamers are strongly fluorescent in an extended random conformation, but the fluorescence is completely quenched upon folding to a helical conformation due to the compact stacking between aryl planes in the backbone. Anion binding disturbs the helical conformation, thus regenerating the fluorescence of the foldamers. This unique property has been utilized to develop a fluorescence turn-on probe for anions such as sulfate and fluoride.</P>
Aromatic Hybrid Foldamer with a Hydrophilic Helical Cavity Capable of Encapsulating Glucose
Hwang, Ji Young,Jeon, Hae-Geun,Choi, Ye Rin,Kim, Junyoung,Kang, Philjae,Lee, Seungwon,Jeong, Kyu-Sung THE AMERICAN CHEMICAL SOCIETY 2017 ORGANIC LETTERS Vol.19 No.20
<P>An indolocarbazole–naphthyridine hybrid oligomer capable of adopting a stable helical conformation was prepared, and its folding properties were thoroughly studied in the solid state and in solution. As a result of folding, a hydrophilic cavity was generated inside the helix wherein monosaccharides were able to be encapsulated in the order of glucose (9.6 × 10<SUP>4</SUP> M<SUP>–1</SUP>) > galactose (1.0 × 10<SUP>4</SUP> M<SUP>–1</SUP>) ≫ mannose (∼0) in 10% (v/v) DMSO/CH<SUB>2</SUB>Cl<SUB>2</SUB>.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/orlef7/2017/orlef7.2017.19.issue-20/acs.orglett.7b02768/production/images/medium/ol-2017-02768x_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/ol7b02768'>ACS Electronic Supporting Info</A></P>
Choi, Hae Yoon,Park, Ji Woo,Lee, Namseok,Hwang, Soon Young,Cho, Geum Ju,Hong, Ho Cheol,Yoo, Hye Jin,Hwang, Taek Geun,Kim, Seon Mi,Baik, Sei Hyun,Park, Kyong Soo,Youn, Byung-Soo,Choi, Kyung Mook American Diabetes Association 2013 Diabetes care Vol.36 No.10
<P><B>OBJECTIVE</B></P><P>To examine the effect of a combined exercise program on C1q/tumor necrosis factor-related protein (CTRP) 3 and CTRP-5 levels and novel adiponectin paralogs suggested to be links between metabolism and inflammation and to evaluate sex differences and association with cardiometabolic risk factors in humans with use of a newly developed ELISA.</P><P><B>RESEARCH DESIGN AND METHODS</B></P><P>This cross-sectional study explored the implications of CTRP-3 and CTRP-5 on cardiometabolic parameters in 453 nondiabetic Korean adults. In addition, we evaluated the impact of a 3-month combined exercise program on CTRP-3 and CTRP-5 levels in 76 obese women. The exercise program consisted of 45 min of aerobic exercise at an intensity of 60–75% of the age-predicted maximum heart rate (300 kcal/session) and 20 min of resistance training (100 kcal/session) five times per week.</P><P><B>RESULTS</B></P><P>Both CTRP-3 and CTRP-5 concentrations were significantly higher in women (<I>P</I> < 0.001) than in men (<I>P</I> = 0.030). In a multiple stepwise regression analysis, CTRP-3 levels were independently associated with age, sex, and triglyceride, LDL cholesterol, adiponectin, and retinol-binding protein 4 (RBP4) levels (<I>R</I><SUP>2</SUP> = 0.182). After 3 months of a combined exercise program, cardiometabolic risk factors, including components of metabolic syndrome, insulin resistance, and RBP4 levels, decreased significantly. In particular, CTRP-3 levels decreased significantly (median [interquartile range] 444.3 [373.8–535.0] to 374.4 [297.2–435.9], <I>P</I> < 0.001), whereas CTRP-5 levels were slightly increased (34.1 [28.6–44.3] to 38.4 [29.8–55.1], <I>P</I> = 0.048).</P><P><B>CONCLUSIONS</B></P><P>A 3-month combined exercise program significantly decreased CTRP-3 levels and modestly increased CTRP-5 levels in obese Korean women.</P>
Bacillus subtilis 발효두유의 AGS 인체 위암세포 증식억제 효과
서혜리(Hae-Ree Seo),김지영(Ji-Young Kim),배근호(Geun-Ho Bae),박건영(Kun-Young Park) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.5
Bacillus subtilis로 발효한 두유의 암세포 증식 저해 효과를 알아보기 위하여 AGS 인체 위암 세포를 이용하여 실험을 수행하였다. 두유와 발효두유 모두 암세포 성장 저해 효과가 나타났으며, 발효두유에서 그 효과가 더욱 증진되었다. 이러한 결과는 apoptosis에 의해 암세포 증식이 저해된 것으로 사료되며 DAPI staining을 통해 뚜렷한 apoptotic body를 관찰할 수 있었다. 발효두유를 처리한 암세포에서 apoptosis를 유발하는 인자인 bcl-2의 발현은 감소하고, 억제하는 인자인 bax의 발현은 발효하지 않은 두유보다 상대적으로 증가하였다. 또한 종양억제 유전자인 p53과 p21의 발현 역시 발효하지 않은 두유보다 발효두유에서 더 증가되었다. 따라서 발효두유의 항암 효과는 이들 유전자의 발현을 조절하여 유도된 apoptosis에 의한 것으로 보이며, 발효두유와 발효하지 않은 두유의 항암효과의 차이는 발효 균주와 발효과정 중 생성된 여러 가지 발효산물들이 암세포 성장 억제에 영향을 끼친 것으로 생각된다. Antiproliferative effects of soy milk fermented with Bacillus subtilis from chungkukjang was studied in AGS human gastric adenocarcinoma cells. The fermented soy milk by B. subtilis (B. subtilis-F-SM) exhibited 82% growth inhibitory effect at 2 ㎎/㎖ concentration, while non-fermented soy milk (Non-F-SM) showed 68%. B. subtilis-F-SM treated AGS cells induced more apoptotic bodies than the Non-F-SM treated cells. In mRNA expressions, B. subtilis-F-SM showed decreased expression of anti-apoptotic bcl-2 and increased expression of pro-apoptotic bax. The expressions of tumor suppressor genes of p53 and p21 were also increased. These results suggest that fermented soy milk by B. subtilis exhibited higher antiproliferative activities compared with non-fermented soy milk.
클라리세이지 향흡입이 치주염환자의 치료 시 통증 및 스트레스에 미치는 효과
유지혜 ( Ji Hye You ),김미영 ( Mi Young Kim ),문혜경 ( Hae Kyung Moon ),민선식 ( Sun Seek Min ),설근희 ( Geun Hee Seol ) 대한예방치과·구강보건학회 2011 大韓口腔保健學會誌 Vol.35 No.1
Objectives. The purpose of this study was to investigate the analgesic and antistress effect of clary sage-inhalation in periodontitis patients. Methods. Thirty-five patients with periodontitis, who were classified under Russell`s Periodontal Index, were studied from September to December 2009. All patients understood the purpose of this study and written informed consent for the study was obtained. Results. Clary sage-inhalation was conducted during the treatment period. Blood pressure, pulse, visual analog scale, and salivary cortisol levels were measured before and after treatment. The visual analog scale score which represents subjective pain perception in the experimental (clary sage-inhalation) group was significantly lower than that in the vehicle (almond oil-inhalation) or control (saline-inhalation) group. In addition, the experimental group showed the lowest systolic and diastolic blood pressure and pulse rate among the three groups. However, there was no significant difference in salivary cortisol level among the three groups. Conclusions. Clary sage-inhalation is effective in alleviating pain and blood pressure. These results suggest that clary sage-inhalation can be a useful method for the relief of pain during the treatment of periodontitis.
Jae Geun Lee,Seon Young Bak,Ji Hae Nahm,Sang Woo Lee,Seon Ok Min,Kyung Sik Kim 한국간담췌외과학회 2015 한국간담췌외과학회지 Vol.19 No.2
Backgrounds/Aims: Stem cell therapies for liver disease are being studied by many researchers worldwide, but scientific evidence to demonstrate the endocrinologic effects of implanted cells is insufficient, and it is unknown whether implanted cells can function as liver cells. Achieving angiogenesis, arguably the most important characteristic of the liver, is known to be quite difficult, and no practical attempts have been made to achieve this outcome. We carried out this study to observe the possibility of angiogenesis of implanted bio-artificial liver using scaffolds. Methods: This study used adipose tissue-derived stem cells that were collected from adult patients with liver diseases with conditions similar to the liver parenchyma. Specifically, microfilaments were used to create an artificial membrane and maintain the structure of an artificial organ. After scratching the stomach surface of severe combined immunocompromised (SCID) mice (n=4), artificial scaffolds with adipose tissue-derived stem cells and type I collagen were implanted. Expression levels of angiogenesis markers including vascular endothelial growth factor (VEGF), CD34, and CD105 were immunohistochemically assessed after 30 days. Results: Grossly, the artificial scaffolds showed adhesion to the stomach and surrounding organs; however, there was no evidence of angiogenesis within the scaffolds; and VEGF, CD34, and CD105 expressions were not detected after 30 days. Conclusions: Although implantation of cells into artificial scaffolds did not facilitate angiogenesis, the artificial scaffolds made with type I collagen helped maintain implanted cells, and surrounding tissue reactions were rare. Our findings indicate that type I collagen artificial scaffolds can be considered as a possible implantable biomaterial.