DNA Repair Gene Polymorphisms Do Not Predict Response to Radiotherapy-Based Multimodality Treatment of Patients with Rectal Cancer: a Meta-analysis

Guo, Cheng-Xian,Yang, Guo-Ping,Pei, Qi,Yin, Ji-Ye,Tan, Hong-Yi,Yuan, Hong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.2

Background: A number of association studies have been carried out to investigate the relationship between genetic polymorphisms in DNA repair genes and response to radiotherapy-based multimodality treatment of patients with rectal cancer. However, their conclusions were inconsistent. The objective of the present study was to assess the role of DNA repair gene genetic polymorphisms in predicting genetic biomarkers of the response in rectal cancer patients treated with neoadjuvant chemoradiation. Materials and Methods: Studies were retrieved by searching the PubMed database, Cochrane Library, Embase, and ISI Web of Knowledge. We conducted a meta-analysis to evaluate the association between genetic polymorphisms and the response in rectal cancer treated with neoadjuvant chemoradiation by checking odds ratios (ORs) and 95% confidence intervals (CIs). Results: Data were extracted from 5 clinical studies for this meta-analysis. The results showed that XRCC1 RS25487, XRCC1 RS179978, XRCC3 RS861539, ERCC1 RS11615 and ERCC2 RS13181 were not associated with the response in the radiotherapy-based multimodality treatment of patients with rectal cancer (p>0.05). Conclusions: This study shows that DNA repair gene common genetic polymorphisms are not significantly correlated with the radiotherapy-based multimodality treatment in rectal cancer patients.

Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites

Guo, Yan-Wu,Guo, Hui-Li,Li, Xing,Huang, Li-Li,Zhang, Bo-Ning,Pang, Xiao-Bin,Liu, Ben-Ye,Ma, Lan-Qing,Wang, Hong 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

In our recent work (Ma et al., in Planta 229(3):457-469, 2009a and 229(4):1077-1086, 2009b), two three-intron type III PKS genes, PcPKS1 and PcPKS2, were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is a three-intron chalcone synthase (CHS) gene, and PcPKS2 is found to be a three-intron benzalacetone synthase (BAS) gene. The regular CHS encoded by a single intron gene have not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3) and a stilbene synthase (STS) gene with three-intron (PcPKS5) were isolated and characterized by functional and phylogenetic analyses. In comparison with PcPKS1, a bifunctional enzyme with both CHS and BAS activity, the enzymatic product of recombinant PcPKS3 was naringenin, bis-noryangonin (BNY) and 4-coumaroyltriacetic acid lactone (CTAL) occurred as side products. The PcPKS5 synthesized resveratrol and a trace amount of naringenin from p-coumaroyl-CoA. To our knowledge, PcPKS5 is the first reported three-intron STS gene in flowering plants. In this work, we speculated that this involved a possible evolutionary route of plant-specific type III PKS superfamily in P. cuspidatum.

Prognostic Significance of Beclin-1 Expression in Colorectal Cancer: a Meta-analysis

Han, Ye,Xue, Xiao-Feng,Shen, Hu-Gang,Guo, Xiao-Bo,Wang, Xu,Yuan, Bin,Guo, Xing-Po,Kuang, Yu-Ting,Zhi, Qiao-Ming,Zhao, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Objective: Beclin-1 has recently been observed as an essential marker of autophagy in several cancers. However, the prognostic role of Beclin-1 in colorectal neoplasia remains controversial. Our study aimed to evaluate the potential association between Beclin-1 expression and the outcome of colorectal cancer patients. Materials and Methods: All related studies were systematically searched in Pubmed, Embase, Springer and Chinese National Knowledge Infrastructure databases (CNKI), and then a meta-analysis was performed to determine the association of Beclin-1 expression with clinical outcomes. Finally, a total of 6 articles were included in our analysis. Results: Our data showed that high Beclin-1 expression in patients with CRC was associated with poor prognosis in terms of tumor distant metastasis (OR=2.090, 95%CI=1.061-4.119, p=0.033) and overall survival (RR=1.422, 95%CI=1.032-1.959, p=0.031). However, we did not found any correlation between Beclin-1 over-expression and tumor differentiation (OR=1.711, 95%CI=0.920-3.183, p=0.090). In addition, there was no evidence of publication bias as suggested by Egger's tests for tumor distant metastasis (p=1.000), differentiation (p=1.000) and OS (p=0.308). Conclusions: Our present meta-analysis indicated that elevated Beclin-1 expression iss associated with tumor metastasis and a poor prognosis in patients with CRC. Beclin-1 might serve as an efficient prognostic indicator in CRC, and could be a new molecular target in CRC therapy.

Simultaneous distillation-extraction for manufacturing ultra-high-purity electronic-grade octamethylcyclotetrasiloxane (D4)

Wenhui Guo,Shuhu Guo,Xu Zhao,Zhenjun Yuan,Yu Zhao,Xin Chang,Hong Li,Xiong Zhao,Ye Wan,Dazhou Yan,Zhongyuan Ren,Xiaolei Fan,Xin Gao 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-

Ultra-high-purity (UHP) electronic-grade octamethylcyclotetrasiloxane (D4) is the key precursor of lowdielectricconstant (low-k) SiCOH films to manufacture integrated circuits (IC), meeting the stringentrequirements of the rapidly developing semiconductor industry. Commonly, metallic impurities in D4were removed by multiple unit operations of adsorption, filtration, and distillation, which could reducethe concentration of a single metallic impurity below 1 ppb. However, D4 with higher purity is requiredby semiconductor production due to an increase in transistor density. Herein, a novel method based onthe integrated simultaneous distillation–extraction (SDE) was developed for manufacturing UHPelectronic-grade D4. The lab and pilot scale experiments showed that the purity of water and D4 has apositive correlation. Based on the experimental data, a double-column process, consisting of azeotropic/extractive distillation column and precision distillation column with UNIQUAC method, was establishedto access the feasibility of scaling up the SDE process. According to the simulation results, D4with the purity > 99.999 wt.% and total metallic impurities (TMI) content below 1 ppb could be obtainedusing ultra-pure water.

Study of Credibility Evaluation of Dynamic Models and their Parameters

Guo, Weimin,Ye, Xiaohui,Zhong, Wuzhi,Su, Yi,Tang, Yaohua,Song, Xinli,Liu, Tao The Korean Institute of Electrical Engineers 2014 The Journal of International Council on Electrical Vol.4 No.1

The time-domain simulation plays an important role in planning, designing and operation of the power system, but mismatches between its results and the actual system response show that the credibility evaluation of dynamic models is critical in all the above aspects of power systems. This paper discusses the technologies of credibility evaluation, including hybrid dynamic simulation with PMU measurements, error analysis and the calculated indices. Moreover, a phase shift method is presented to implement hybrid dynamic simulation in PSD-FDS. Then the paper proposes a credibility evaluation scheme to provide solution for the evaluation. At last, one sample is used to validate the implementation of hybrid dynamic simulation, while the other sample illustrates the effectiveness of the credibility evaluation approach.

Imidazole derivatives for efficient organic light-emitting diodes

Ye Shaofeng,Zhuang Shaoqing,Pan Biao,Guo Runda,Wang Lei 한국정보디스플레이학회 2020 Journal of information display Vol.21 No.3

Since the first development of organic light-emitting diodes (OLEDs) in 1987, imidazole derivatives, mainly including phenanthroimidazole (PI) and benzimidazole (BI), have increasingly attracted attention. Their strong electron-withdrawing properties make them suitable for emitters, hosts, and electron-transporting materials (ETMs). In this review, an overview of the recent developments regarding OLEDs based on imidazole derivatives, especially the relationship between the molecule structure and the device performance as fluorescent and host materials, is given.

A Novel Strategy for Thermostability Improvement of Trypsin Based on N-Glycosylation within the Ω-Loop Region

Guo, Chao,Liu, Ye,Yu, Haoran,Du, Kun,Gan, Yiru,Huang, He The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.7

The Ω-loop is a nonregular and flexible structure that plays an important role in molecular recognition, protein folding, and thermostability. In the present study, molecular dynamics simulation was carried out to assess the molecular stability and flexibility profile of the porcine trypsin structures. Two Ω-Loops (fragment 57-67 and fragment 78-91) were confirmed to represent the flexible region. Subsequently, glycosylation site-directed mutations (A73S, N84S, and R104S) were introduced within the Ω-loop region and its wing chain based on its potential N-glycosylation sites (Asn-Xaa-Ser/Thr consensus sequences) and structure information to improve the thermostability of trypsin. The result demonstrated that the half-life of the N84S mutant at 50℃ increased by 177.89 min when compared with that of the wild-type enzyme. Furthermore, the significant increase in the thermal stability of the N84S mutant has also been proven by an increase in the T_m values determined by circular dichroism. Additionally, the optimum temperatures of the wild-type enzyme and the N84S mutant were 75℃ and 80℃, respectively. In conclusion, we obtained the thermostability-improved enzyme N84S mutant, and the strategy used to design this mutant based on its structural information and N-linked glycosylation modification could be applied to engineer other enzymes to meet the needs of the biotechnological industry.

Tristetraprolin Overexpression in Gastric Cancer Cells Suppresses PD-L1 Expression and Inhibits Tumor Progression by Enhancing Antitumor Immunity

Guo, Jian,Qu, Huiheng,Shan, Ting,Chen, Yigang,Chen, Ye,Xia, Jiazeng Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.7

The RNA-binding protein tristetraprolin (TTP) binds to adenosine-uridine AU-rich elements in the 3'-untranslated region of messenger RNAs and facilitates rapid degradation of the target mRNAs. Therefore, it regulates the expression of multiple cancer and immunity-associated transcripts. Furthermore, a lack of TTP in cancer cells influences cancer progression and predicts poor survival. Although the functions of TTP on cancer cells have previously been researched, the mechanism of TTP on the interaction between cancer cells with their micro-environment remains undiscovered. In this study, we admed to determine the role of cancer cell TTP during the interaction between tumor and immune cells, specifically regulatory T cells (Tregs). We evaluate the capability of TTP to modulate the antitumor immunity of GC and explored the underlying mechanism. The overexpression of TTP in GC cells dramatically increased peripheral blood mononuclear lymphocyte (PBML) -mediated cytotoxicity against GC cells. Increased cytotoxicity against TTP-overexpressed GC cells by PBMLs was determined by Treg development and infiltration. Surprisingly, we found the stabilization of programmed death-ligand 1 (PD-L1) mRNA was declining while TTP was elevated. The PD-L1 protein level was reduced in TTP-abundant GC cells. PD-L1 gas been found to play a pivotal role in Treg development and functional maintenance in immune system. Taken together, our results suggest the overexpression of TTP in GC cells not only affects cell survival and apoptosis but also increases PBMLs -mediated cytotoxicity against GC cells to decelerate tumor progression. Moreover, we identified PD-L1 as a critical TTP-regulated factor that contributes to inhibiting antitumor immunity.

Timosaponin AIII induces apoptosis and autophagy in human melanoma A375-S2 cells

Ye Wang,Lei Xu,Li-Li Lou,Shao-Jiang Song,Guo-Dong Yao,Meng-Yao Ge,Toshihiko Hayashi,Shin-ichi Tashiro,Satoshi Onodera,Takashi Ikejima 대한약학회 2017 Archives of Pharmacal Research Vol.40 No.1

Timosaponin AIII (AIII), a steroidal saponinisolated from Anemarrhena asphodeloides Bge. Our studyshowed that AIII induced both apoptosis and autophagy,and autophagy inhibited apoptosis in A375S2 cells. Furtherly,this study was carried out to investigate what kindof cytokines plays an important role in this process. Theresults revealed that AIII induced apoptosis through activatingc-Jun N-terminal protein kinase (JNK) or extracellularsignal related kinase (ERK) signaling pathway andgenerating NO. However, JNK or ERK inhibited autophagy,while NO had no effect on autophagy. Therefore,JNK, ERK or NO regulates two programmed death processesin different ways. AIII did not show obvious cytotoxiceffect on human peripheral blood mononuclear cells,which indicated that AIII has less side effects on normalcells, and could be considered as a leading compound fordeveloping novel anticancer drug.

A critical review on ammonium recovery from wastewater for sustainable wastewater management

Ye, Yuanyao,Ngo, Huu Hao,Guo, Wenshan,Liu, Yiwen,Chang, Soon Woong,Nguyen, Dinh Duc,Liang, Heng,Wang, Jie Elsevier 2018 Bioresource technology Vol.268 No.-

<P><B>Abstract</B></P> <P>The growing global population’s demand for ammonium has triggered an increase in its supply, given that ammonium plays a crucial role in fertilizer production for the purpose of food security. Currently, ammonia used in fertilizer production is put through what is known as the industrial Haber Bosch process, but this approach is substantially expensive and requires much energy. For this reason, looking for effective methods to recover ammonium is important for environmental sustainability. One of the greatest opportunities for ammonium recovery occurs in wastewater treatment plants due to wastewater containing a large quantity of ammonium ions. The comprehensively and critically review studies on ammonium recovery conducted, have the potential to be applied in current wastewater treatment operations. Technologies and their ammonium recovery mechanisms are included in this review. Furthermore the economic feasibility of such processes is analysed. Possible future directions for ammonium recovery from wastewater are suggested.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Growing global population ultimately calls for ammonium recovery. </LI> <LI> Wastewater is considered as the renewable resource for the ammonium recovery. </LI> <LI> The bioelectrochemical system can efficiently and effectively recover ammonium. </LI> <LI> Mechanism and technology of ammonium recovery were summarized. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>