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Giang Thi Huong Luong,Byoung-Su Yoon 한국양봉학회 2015 韓國養蜂學會誌 Vol.30 No.1
Black queen cell virus is one of the members belonging to Picornavirus, which possesses a 3C-like protease. Viral replication and capsid assembly in the order Picornavirales require polyprotein proteolysis processing, which is performed by 3C or 3C-like (3CL) proteases. Therefore, viral proteases are attractive targets for anti-viral therapy. The complete 3C-like protease gene from Black queen cell virus was cloned into pGEM-3Zf(+) vector, and the amino acid sequence revealed a high homology (99.1%) to 3C-like protease deposited in a reference Genbank EF517515.1. For the expression, 3C-like protease gene was subcloned into pMAL-C2 vector system. The expression level of MBP-BQCV-3CL protein was evaluated under different standards of cell optical density, IPTG concentration, induction time, and induction temperature. As the result, the optimal condition was confirmed as the induction with 0.1mM IPTG at 25°C for 6 hours, and a purification step using amylose resin reached a high protein yield of approximately 2.22mg per 1ml of cultured cells. Purified MBP-BQCV-3CL proteins can provide an important antigen source to generate monoclonal antibody and will become a potential candidate for anti-viral drugs, which can inactivate 3C-like protease function as well as BQCV replication.
Giang Thi Huong Luong,Joo-Seong Lee,So-Jung Yong,Byoung-Su Yoon 한국양봉학회 2015 韓國養蜂學會誌 Vol.30 No.3
Black Queen Cell Virus (BQCV) is one of the most prevalent viruses in honeybee, and could not be recognized by any infected symptoms in the adult bees. Thus, a newly developed method for rapid RNA isolation and cDNA generation from honeybee samples will be useful and easy to evaluate the presence of BQCV. A significant improvement by only one-step RNA isolation for 10 minutes made possible to detect BQCV RNAs at high quantity. cDNAs were also generated directly from isolated BQCV RNAs by different primer sets for 1 minutes and were subsequently applied to Ultra-Rapid Real-Time PCR by using microchip of 6μl reaction volume with extremely short time in each step of PCR. This system provided the ultra-high speed reaction (30 cycles in less than 10 min) including melting temperature analysis for amplified BQCV products. These results suggest that BQCV detection can be completed within 6 min 42 sec, and the Ultra-Rapid Real-time PCR is sensitive, reliable and time-saving for monitoring BQCV in honeybee.
Giang Huong Thi Luong,Byoungsu Yoon 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
Black queen cell virus (BQCV), one of the most prevalent viruse, causes the death of queen larvae and pupae. The RNA-dependent RNA polymerases (RdRPs) are central components in the life cycle of RNA viruses that catalyzes the replication of RNA from an RNA template without DNA stage. Inhibition of RdRP gene is importantly significant for application of monoclonal antibody generation as a diagnosis tool for identifying BQCV infection in honey bee..In this study, the presence of BQCV in honey bee samples was confirmed by PCR using BQCV F/R primer set to multiply of 700 bp DNA fragment. For ampification of BQCV Rdrp gene, a primer set attached BamHI/SalI restriction site was designed based on the best homogenization between BQCV RdRP sequences in NCBI, a PCR product containing BQCV RdRP gene with 1576 bp in length was amplified. Furthermore, BQCV RdRP gene will be cloned into pBlueXcm vector for future researches.
Giang Thi Huong Luong,Hee-Young Lim,Byoung-Su Yoon 한국양봉학회 2014 韓國養蜂學會誌 Vol.29 No.3
Honeybee populations infected by numerous viruses could not easily distinguish from the healthy populations because there are no apparent clinical symptoms and signs of infection. Black queen cell virus (BQCV) is one of the most prevalent viruses, resulting in the death of queen larvae and pupae. Like to other picornaviruses, BQCV has the RNA dependent RNA polymerase (RdRp) that catalyzes the replication of viral RNA. The optimal conditions for expression of MBP-BQCV-RdRp protein were confirmed by an induction with 0.1mM IPTG at 25°C for 6 hours and the MBP-BQCV-RdRp protein was purified using by amylose resin. Total yield of protein reached approximately 5mg per 50ml of induced cells. Due to its property that is an essential enzyme of virus replication, purified recombinant BQCV-RdRp proteins can be provided to the further lead as an important source to study how to control the BQCV viral disease in honeybee, development of a rapid kit for the diagnosis of BQCV infection in field,or finding out the anti-viral chemical compound for the inhibitor assay to prevent BQCV replication in honeybee.
Le, Thi Huong,Nga, Thi Thu Vu,Nguyen, Ngoc Dung,Le, Thi Thanh Xuan,Kim, Bao Giang,Phan, Thi Hai,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Tuan Lam,Hoang, Van Minh,Pham, Thi Quynh Nga Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1
The aim of this paper is to report the rate of current and ever cigarette smoking and explore correlates of current cigarette smoking among adolescents aged 13-15 in Viet Nam. This analysis was derived from GYTS survey, which comprised of 3,430 adolescents aged 13-15, conducted in 2014 in 13 cities and provinces of Viet Nam. We calculated the weighted rates of current and ever cigarette smoking and reported patterns of smoking behavior. We also performed logistic regression to explore correlates of current cigarette smoking behavior. The weighted rate of ever cigarette smoking was 9.5% (95% confidence interval (CI): 8.5 %-10.5%), in which the weighted rate among males (15.4%; 95% CI: 13.6%-17.0%) was higher than that among females (4.2%; 95% CI: 3.3%-5.1%). The weighted rate of current cigarette smoking was relatively low at 2.5% (95%CI: 2.0%-3.0%) with higher weighted rate among males (4.9%; 95% CI: 3.8%-5.9%) compared to the corresponding figure among females (0.2%; 95% CI: 0.0 %-0.5%). Current cigarette smoking was significantly higher among males than females, in students aged 15 versus 13 years old, and in students who had several or all close friends smoking and students with daily observation of smoking at school. For greater smoking reduction outcomes, we recommend that tobacco interventions for adolescents should consider targeting more male students at older ages, establish stricter adherence to school-based banning of cigarette smoking, engage both smoking and nonsmoking adolescents and empower adolescents to resist peer smoking influence as well as changing their norms or beliefs towards smoking benefits.
VO, Thi Huong Giang,LUONG, Duy Binh,LE, Khoa Huan Korea Distribution Science Association 2022 The Journal of Asian Finance, Economics and Busine Vol.9 No.6
With the dramatic increase in mobile usage, more and more businesses see the potential of m-commerce. This study focuses on a subcategory of m-commerce, a mobile shopping application. To understand the purchase intention via m-commerce applications, this study is aimed to identify the main factors that are related to the applications and explore the influence of these factors on consumers' mobile shopping intention. This study uses quantitative research methods and selects Vietnam as its case study. The survey responses of 450 Vietnamese mobile shoppers were analyzed using partial least squares structural equation modeling (PLS-SEM). The results indicated that online reviews, e-service quality, and information quality are significant predictors of behavior intention, and perceived risk negatively influences consumer online purchase intention via the applications. The content enriches the combined research of detailed and possible models with quality dimensions and risk perception. Practitioners such as e-retailers and developers can enhance the quality of applications and determine strategies to reach potential users and maximize revenue. M-commerce providers should pay adequate attention to credible and influential online reviews since mobile shoppers heavily rely on reading reviews before buying a product.
Nguyen, Thanh Huong,Nguyen, Trung Kien,Kim, Bao Giang,Hoang, Van Minh,Phan, Thi Hai,Doan, Thu Huyen,Luong, Ngoc Khue,Nguyen, Thuy Linh,Nguyen, Tuan Lam,Pham, Thi Quynh Nga Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.no.sup1
Studies have shown that smoking is a learnt behavior, often initiated during adolescence. This paper aims to describe tobacco-related knowledge, attitude and associations among school adolescents aged 13-15 with exposure to anti-smoking information. Using data from the Global Youth Tobacco Survey (GYTS) in Viet Nam, 2014, knowledge was measured through 4 questions about tobacco use, and attitude was assessed through 3 questions on personal, social and environmental aspects. Students giving most anti-tobacco responses to all questions were considered as having correct knowledge or appropriate attitude or both. Access to anti-smoking information was determined by exposure to any media messages on tobacco control during the past 30 days and teaching in school about the danger of tobacco use during the past 12 months. A substantial percentage of students thought that being near others who smoke might be harmful to them and smoking is harmful to health (89.4% and 89.6% respectively). However, only 46.4% reported that it is definitely difficult to quit smoking and 66.9% thought that smoking for only 1 or 2 years, once stopped, is harmful to health. Slightly more than half of the respondents reported appropriate attitude that young smokers have fewer friends than others and smoking makes them less attractive and less comfortable at social events. Noticing anti-smoking messages in the media together with having lessons in school about the dangers of tobacco substantially increased the likelihood of having correct knowledge, appropriate attitude and both. Despite relatively high awareness about smoking harms, effective educational communication is still highly needed to improve the level of comprehensive knowledge and an appropriate attitude regarding tobacco use.
역전사 실시간 Recombinase Polymerase Amplification (RT/RT RPA)에 의한 꿀벌 Black Queen Cell Virus의 신속 검출
임수진(Su Jin Lim),Giang Thi Huong Luong,민상현(Sang Hyun Min),왕지희(Ji Hee Wang),윤병수(Byoung-Su Yoon) 한국양봉학회 2016 韓國養蜂學會誌 Vol.31 No.1
Black Queen Cell Virus (BQCV) is one of pathogenic virus in honeybee which could be detected using reverse transcription real-time PCR (RT/RT-PCR). In this study, for rapid detection of BQCV, Recombinase Polymerase Amplification (RPA) was applied, and BQCV-specific reverse transcription real-time RPA (RT/RT-RPA) method was newly developed based on BQCV-specific RT/RT PCR. The Real-time RPA (RT-RPA) was performed at 37°C isothermal condition for 40 minutes. During the experiments, specific DNA amplifications were real-timely monitored using fluorescent detector. BQCV-specific DNA amplification could be detected from 3 min 26 sec after RPA reaction with specific DNA templates by RT-RPA, while 41 min 42 sec was required by qRTPCR with same quantities of initial templates. With generated cDNA from BQCV-infected honeybee, specific DNA amplification was recognized at 4 min 18 sec using RT-RPA, however, 66 min 5 sec was needed using Real-time PCR. Moreover, with reverse transcriptase and RPA solution, BQCVspecific DNA amplification could be detected at 8 min 36 sec from total RNA of BQCV-infected honeybee using one-step Reverse Transcription/Real-Time RPA (RT/RT-RPA).