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Synthesis of Oligosaccharide-containing Orange Juice Using Glucansucrase
탄한,서예슬,조재영,이선,Ghahyun J. Kim,윤종원,안승현,황경환,박준승,장태수,김도만 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3
Orange juice is a well-accepted fruit juice, and is a natural source of various vitamins, especially vitamin C, as well as sugar, potassium, thiamine, folate, flavonoids and antioxidants. The respective fructose, glucose, and sucrose concentrations were 9.3, 22.9, and 48.1 g/L in the original orange juice used in this study, and 183.4, 170.1, and 142.8 g/L after concentration. Over 97% of the sucrose in the juice was enzymatically converted to glucooligosaccharides upon addition of 3 U/mL dextransucrase, prepared from Leuconostoc mesenteroides 512FMCM, at 16oC. The synthesized oligosaccharides comprised 35.0% of the total saccharides in the concentrated juice and 31.7% in the original juice. The optimum conditions for oligosaccharide synthesis using the concentrated juice were 35.2 × 10−1 U/mL dextransucrase and 1% Ca(OH)2. The calories in the original and modified concentrated orange juices were 325.4 and 246.7 kcal/L, respectively. Compared to the original concentrated juice, the enzyme-modified concentrated juice prevented the formation of 62.7% of the insoluble glucan resulting from addition of mutansucrase, produced by Streptococcus mutans.
Enhanced Saccharification of Rice Straw Using Hypochloritehydrogen Peroxide
강희경,Nahyun M. Kim,Ghahyun J. Kim,Eun-Seong Seo,Hwa-Ja Ryu,Sang-Il Yun,Hyun-Chul Choi,Donal F. Day,Jongho Kim,Dong-Lyun Cho,김도만 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.2
Rice straw is a lignocellulosic biomass, and has been recognized as a renewable organic substance and alternative energy source. In this study, rice straw was pretreated with hypochlorite-hydrogen peroxide (Ox-B)solution. The optimal pretreatment conditions were determined via response surface methodology, and the pretreated rice straw was hydrolyzed with exo-glucanase, endoglucanase,hemicellulase, and β-glucosidase Accellerase 1000™ (endo-glucanase equivalent activity of 1,250 carboxy methyl cellulose (CMC) U/g of rice straw pretreated for 24h). The optimal conditions were as follows: 60 min pretreatment using Ox-B solution containing 0.6% hypochlorite and 25% hydrogen peroxide for 1 g of rice straw in a total reaction volume of 240 mL. Under these conditions, 406.8mg of D-glucose and 224.0 mg of D-xylose were obtained from 1 g of rice straw. The fermentation of enzymatic hydrolysates containing 8.14 g/L D-glucose and 4.49 g/L D-xylose with Pichia stipitis generated 3.65 g/L of ethanol with a corresponding yield of 0.37 g/g. The maximum possible ethanol conversion rate is 72.54%.