Anti-diabetic Potential of Silver Nanoparticles Synthesized with Argyreia nervosa Leaf Extract High Synergistic Antibacterial Activity with Standard Antibiotics Against Foodborne Bacteria

Saratale, Ganesh Dattatraya,Saratale, Rijuta Ganesh,Benelli, Giovanni,Kumar, Gopalakrishnan,Pugazhendhi, Arivalagan,Kim, Dong-Su,Shin, Han-Seung Springer-Verlag 2017 Journal of cluster science Vol.28 No.3

<P>The current investigation highlighted a novel cost-effective green synthesis of silver nanoparticles (AgNPs) using Argyreia nervosa leaves extract (ANE) as a potential reducing and capping agent. Surface plasmon resonance confirmed the formation of AgNPs with maximum absorbance at lambda (max) = 435 nm. FTIR revealed the involvement of biological macromolecules of ANE in the synthesis and stabilization of AgNPs. HRTEM images showed that the size of the spherical AgNPs ranged between 5 and 40 nm with average particle size of about 15 nm. The ANE-AgNPs showed inhibition activity against carbohydrate digestive enzymes alpha-amylase and alpha-glucosidase, with EC50 of 55.5 and 51.7 A mu g/mL, respectively, indicating its antidiabetic potential. The in vitro antioxidant activity of ANE-AgNPs was evaluated in terms of ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and DPPH (1,1-diphenyl-2-picrylhydrazyl) free radicals scavenging assays with IC50 value of 44.3 and 55.9 A mu g/mL, respectively. The AgNPs displayed strong antibacterial activity against foodborne bacteria with zone of inhibition 16.0 and 12.5 mm for Escherichia coli and Staphylococcus aureus, respectively, and also exhibited strong synergistic antibacterial activity together with standard antibiotics. The biological activity in terms of antioxidant, antidiabetic and antibacterial potential could be useful in various bio-applications such as cosmetics, food, and biomedical industry.</P>

Screening and optimization of pretreatments in the preparation of sugarcane bagasse feedstock for biohydrogen production and process optimization

Saratale, Ganesh Dattatray,Saratale, Rijuta Ganesh,Kim, Sang Hyoun,Kumar, Gopalakrishnan Elsevier 2018 International journal of hydrogen energy Vol.43 No.25

<P><B>Abstract</B></P> <P>This work evaluated the effects of individual alkaline, sodium carbonate (Na<SUB>2</SUB>CO<SUB>3</SUB> denoted as; NaC), sodium sulfide (Na<SUB>2</SUB>SO<SUB>3</SUB> denoted as; NaS) and combination of NaC + NaS pretreatment for the saccharification of sugarcane bagasse (SCB). The effects of different pretreatments on chemical composition and structural complexity of SCB in relation with its saccharification were investigated. For enzymatic hydrolysis of pretreated SCB we have utilized the produced crude enzymes by <I>Streptomyces</I> sp. MDS to make the process more cost effective. A enzyme dose of 30 filter paperase (FPU) produced a maximum reducing sugar (RS) 592 mg/g with 80.2% hydrolysis yield from NaC + NaS pretreated SCB under optimized conditions. The resulted enzymatic hydrolysates of each pretreated SCB were applied for hydrogen production using <I>Clostridium beijerinckii</I> KCTC1785. NaC + NaS pretreated SCB hydrolysates exhibited maximum H<SUB>2</SUB> production relative to other pretreatment methods. Effects of temperature, initial pH of culture media and increasing NaC + NaS pretreated SCB enzymatic hydrolysates concentration (2.5–15 g/L) on bioH<SUB>2</SUB> production were investigated. Under the optimized conditions, the cumulative H<SUB>2</SUB> production, H<SUB>2</SUB> production rate, and H<SUB>2</SUB> yield were 1485 mL/L, 61.87 mL/L/h and 1.24 mmol H<SUB>2</SUB>/mol of RS (0.733 mmol H<SUB>2</SUB>/g of SCB), respectively. The efficient conversion of the SCB hydrolysate to H<SUB>2</SUB> without detoxification proves the viability of process for cost-effective hydrogen production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pretreatments of alkaline, sodium carbonate, sodium sulfide and combination demonstrated for sugarcane baggase. </LI> <LI> Several crucial factors towards optimization were carried out. </LI> <LI> Peak 59.2 g/L of reducing sugar (RS) with 80.2% hydrolysis yield from NaC + NaS pretreated SCB. </LI> <LI> Significant hydrogen production and process optimization using SCB hydrolysates studied in detail. </LI> </UL> </P>

Electrochemical Oxidation of Phenol for Wastewater Treatment Using Ti/PbO2 Electrode

Saratale, Rijuta Ganesh,Hwang, Kyoung-Jin,Song, Ji-Young,Saratale, Ganesh Dattatray,Kim, Dong-Su American Society of Civil Engineers 2016 Journal of environmental engineering Vol.142 No.2

<P>The electrochemical oxidation of phenol was studied using a Ti/PbO2 electrode prepared by the electrodeposition method with PbO2 coated on Ti. The structural and morphological activity of Ti/PbO2 was analyzed by scanning electron microscopy (SEM) and X-ray diffraction (XRD), and intermediates formed after degradation of phenol were quantitatively assessed by high-pressure liquid chromatography (HPLC). Optimization of various parameters such as current density, initial phenol concentration, initial solution pH, and different temperature and dose of Fe2+ on electrochemical degradation of phenol using Ti/PbO2 were investigated. Complete removal of phenol (250mgL-1) was observed at 50 degrees C, potential difference (5V), and at pH 2. Experimental results showed that the phenol removal rate increased with increasing current intensity along with significant reduction in total organic carbon (TOC). Fundamental kinetic data obtained for the degradation of phenol by Ti/PbO2 was found to follow in accordance with the zero-order kinetics with respect to the phenol concentration. This paper is expected to be useful for the development of electrochemical process using Ti/PbO2 for the degradation of phenol containing wastewater. (C) 2015 American Society of Civil Engineers.</P>

Combined effect of inorganic salts with calcium peroxide pretreatment for kenaf core biomass and their utilization for 2,3-butanediol production

Saratale, Rijuta Ganesh,Shin, Han Seung,Ghodake, Gajanan S.,Kumar, Gopalakrishnan,Oh, Min Kyu,Saratale, Ganesh Dattatraya Elsevier 2018 Bioresource technology Vol.258 No.-

<P><B>Abstract</B></P> <P>This study focuses on development of calcium peroxide (CaO<SUB>2</SUB>) pretreatment that removes major part of lignin but retaining most of sugar components of kenaf core powder (KCP) biomass. In chemical pretreatment, usually higher loss of biomass occurs which was less during this pretreatment strategy. Supplementation of inorganic salts; manganese sulfate (MnSO<SUB>4</SUB>) and cobalt chloride (COCl<SUB>2</SUB>) in CaO<SUB>2</SUB> pretreatment resulted in maximum delignification of KCP relative to individual CaO<SUB>2</SUB> pretreatment. Maximum glucose yield (98%) and hydrolysis yield (80.5%) was achieved after enzymatic hydrolysis (30 FPU/g of KCP) under optimized conditions. Analytical results proved effective lignin removal and significant destruction of KCP with this pretreatment strategy. Finally, utilization of KCP enzymatic hydrolysates by developed strain <I>Klebsiella pneumoniae</I> KMK05 resulted in maximum 2,3-butanediol (BDO) production (10.42 g/L) and BDO titer (0.385 g/g of sugar). BDO titer achieved with KCP derived sugars were found comparable with the mixture of standard sugars which is notable.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Developed CaO<SUB>2</SUB> pretreatment & optimized various operational parameters for KCP biomass. </LI> <LI> Addition of inorganic salts improved delignification, glucose yield and total sugar recovery. </LI> <LI> Maximum BDO production (10.42 g/L) was achieved using <I>Klebsiella pneumoniae</I> KMK05. </LI> <LI> This novel pretreatment strategy abstained loss of sugar components of biomass. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Blood management strategies in congenital Glanzmann thrombasthenia at a hematology referral center

Ganesh Kasinathan,Jameela Sathar 대한혈액학회 2021 Blood Research Vol.56 No.4

Background Glanzmann thrombasthenia is associated with abnormalities in the glycoprotein IIb/IIIa receptor. This study, conducted at Ampang Hospital, Malaysia, aimed to assess outcomes of blood management strategies for Glanzmann thrombasthenia. Methods Ten patients with Glanzmann thrombasthenia aged 9 years (2009‒2018) were examined. Data on clinical characteristics, transfusion practices, and patient blood management were obtained from medical records. Patient blood management methods included parenteral iron, erythropoietin, hormonal pills, intrauterine progesterone contraceptive devices, tranexamic acid, and recombinant factor VIIa. Primary outcomes were hemoglobin levels and the proportion of patients who received blood transfusion. Secondary outcomes were morbidity and mortality. Results The median age at diagnosis was 8.2 years (range, 1‒15 yr). The female-to-male ratio was 9:1. Eight patients had type 2 disease (5‒20% of normal GPIIb/IIIa), and two patients had type 1 disease (normal GPIIb/IIIa <5%). All patients had iron deficiency. All female patients presented with significant menorrhagia. Other bleeding symptoms included epistaxis, spontaneous skin bruising, hemoptysis, gingival bleeding, knee hemarthrosis, and pelvic hematoma. No patient experienced life-threatening bleeding. Our patients had a mean hemoglobin level of 5.6 g/dL at diagnosis. All patients were optimized using non-transfusion methods as described above. Our patient had a current mean hemoglobin level of 11 g/dL. Approximately 70% (7/10) of patients did not experience receiving blood transfusions in the last 5 years. No patient experienced non-transfusion-related morbidities such as sepsis, thromboembolism, or cardiorespiratory events. Conclusion High cost, transfusion-related adverse events, and immunomodulation could be effectively prevented by avoiding unnecessary blood transfusions.

Wind flow modification by a jet roof for mitigation of snow cornice formation

Ganesh Kumar,Ajay Gairola,Aditya Vaid 한국풍공학회 2021 Wind and Structures, An International Journal (WAS Vol.32 No.2

The snow cornice mass on the formation zone had triggered avalanches which led to the loss of human life and property. Snow cornice is formed due to flow separation on the leeward side. Effect of lee slope is more prominent in the formation of snow cornices as compared to the windward slope. The analysis of wind flow pattern has been carried out to evaluate the performance of a jet roof. Computational Fluid Dynamics (CFD) analysis of wind flow over a 2D hill model was carried out using RNG based k- turbulence models available in ANSYS Fluent. Effect of varying leeward hill slope (1:2 to 1:6) on flow separation for the given windward slope was observed and a critical slope of 1:4 was found at which the separation zone ceased to exist. The modification of wind flow over a hill due to the installation of jet roof was simulated. It was observed that jet roof had significantly modified the wind flow pattern around hill ridgeline and ultimately snow cornice formation had mitigated. The results of the wind flow pattern were validated with the wind data collected at the experimental site, Banihal Top (Jammu and Kashmir, India). The wind flow simulation over the hill and mitigation of cornice formation by the jet roof has been explained in the present paper.

The question of equal representation of citizens in the legislature of Nepal: rhetoric and reality

Ganesh Prasad Pandeya,Tatsuo Oyama 서울대학교행정대학원 2019 Asian Journal of Political Science Vol.27 No.1

To what extent the legislature represents the views of all social groups is a central question in multiethnic societies like Nepal. The results from descriptive and inferential statistical analysis of all representatives from 1959 to 2013 show the existence of a prolonged unequal representation of caste/ethnic groups in the legislature of Nepal. A few subgroups of peoples have historically created a ‘creamy layer’ throughout all political systems. Although women and some excluded identities have gained substantial seats in the later period, progress for many groups, particularly historically marginalized ones, has been far too slow. The representation patterns are largely associated with a design of political institutions, such as electoral system and ethnic quotas, as well as the socioeconomic status of a group linked to poverty, wealth, adult literacy, and human development. These findings suggest that the chances of equal representation appear not just to the worth of group effort rather than its ability to influence the political process through power and resources. Thus, reforming political institutions, particularly the electoral systems, and improving the socioeconomic status of women and excluded identities, all of which demand strong political will and commitment, may narrow down inequality in political representation.

Novel Approach: Testing and Computing Periodicity of Continuous Time Signal

Ganesh Gautam,Surendra Shrestha 한국디지털융합학회 2017 IJICTDC Vol.2 No.1

Periodic signals are those which repeat itself after certain interval of time. The time after which it repeats is called as the period of the signal. Periodic signals are used almost everywhere in application of electrical engineering such as, supply of electricity means supplying AC voltage constantly. This supply of ac is periodic waveform [1]. Continuous time sinusoids are periodic if they are not truncated and period of this type of signal can be predicted in advanced just comparing the angular frequency (w). Whenever addition or multiplications of two or more sinusoids or any other continuous time signals are involved then it is not that simple to find the period of the resulting signal [2]. All the cases of addition and multiplication of different types of continuous time signals were taken into account and the result were obtained from MATLAB. Findings were analyzed and generalized being based on the result obtained from MATLAB. When addition of two or more continuous time signals takes place, the period of resulting signal is the Least Common Multiple (LCM) of the period of individual signal. When multiplication of two or more continuous time signal are involved the period of resulting signal is the half the value of Least Common Multiple (LCM) of the period of individual signal, if any one of the signal has both positive and negative amplitude. Else the period of the resulting signal is Least Common Multiple of the period of individual signal. When addition of two or more continuous time signal are involved the period of resulting signal is the value of Least Common Multiple (LCM) of the period of individual signal, even if any one of the signal has both positive and negative amplitude. Generally Continuous time Fourier series is performed to obtain the spectrum either that may be magnitude spectra or phase spectra. Periodicity and the period of the signal are vital for representing time domain signal to frequency domain.

Effect of Alstonia scholaris in Enhancing the Anticancer Activity of Berberine in the Ehrlich Ascites Carcinoma-Bearing Mice

Ganesh Chandra Jagetia,Manjeshwar Shrinath Baliga 한국식품영양과학회 2004 Journal of medicinal food Vol.7 No.2

The chemomodulatory activity of Alstonia scholaris extract (ASE) was studied in combination with berberine hydrochloride (BCL), a topoisomerase inhibitor, in Ehrlich ascites carcinoma-bearing mice. The tumor-bearing animals were injected with various doses of ASE, and 8 mg/kg of BCL (one-fifth of the 50% lethal dose) was combined with different doses of ASE (60–240 mg/kg). The combination of 180 mg/kg of ASE with 8 mg/kg of BCL showed the greatest antitumor effect; the number of tumor-free survivors was more, and the median survival time and the average survival time increased up to 47 and 40.5 days, respectively, when compared with either treatment alone. Similarly, when 180 mg/kg of ASE was combined with different doses of BCL (2–12 mg/kg), a dose-dependent increase in the anticancer activity was observed up to 8 mg/kg of BCL. However, a further increase in the BCL dose to 10 and 12 mg/kg resulted in toxic side effects. The best effect was observed when 180 mg/kg of ASE was combined with 6 or 8 mg/kg of BCL, where an increase in the antineoplastic activity was reported. The efficacy of the combination of 180 mg/kg of ASE was also tested with 6 mg/kg body weight of BCL in various stages of tumorigenesis, and it was effective when given in the early stages, although the efficiency decreased with an increase in the tumor developmental stages.

STUDY OF P-CURVATURE TENSOR IN THE SPACE-TIME OF GENERAL RELATIVITY

( Ganesh Prasad Pokhariyal ),( Sudhakar Kumar Chaubey ) 호남수학회 2023 호남수학학술지 Vol.45 No.2

The P-curvature tensor has been studied in the space-time of general relativity and it is found that the contracted part of this tensor vanishes in the Einstein space. It is shown that Rainich conditions for the existence of non-null electro variance can be obtained by P_αβ. It is established that the divergence of tensor G_αβ defined with the help of P_αβ and scalar P is zero, so that it can be used to represent Einstein field equations. It is shown that for V4 satisfying Einstein like field equations, the tensor P_αβ is conserved, if the energy momentum tensor is Codazzi type. The space-time satisfying Einstein’s field equations with vanishing of P-curvature tensor have been considered and existence of Killing, conformal Killing vector fields and perfect fluid space-time has been established.