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배귀석,남경표,김혜숙,이상구,최행석,민우기,주종원,맹원재,장문백 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6
This study was conducted to determine the effects of the artificial culture medium of wild-ginsengs on in vitro fermentation characteristics. NH_(3)-N concentration was showed the highest in 3% WGM treatment among all treatments and control. In addition, microbila protein synthesis was significantly different in all treatments throughout the incubation time, and WGM 3% tratment was the highest at the 9 h incubation(P<0.05). Protozoa numbers within rumen were decreased in all WGM treatments a t9h incubation time, whereas WGM 3% treatment was always decreased throughtout the incubation(P<0.05). NDF and ADF digestibility showed no significantly increased as the incubation time in both control and treatments. NDF digestibility showed no significantly increased as the incubation time in both control and treatments. NDF digestibility showed no significantly difference between contro and the 3% treatment, and ADF digestibility was similar in all. Total volatile fatty acid(VFA) concentrations of WGM treatments without 5% were significantly higher than control(P<0.05). No differences were observed in total VFA, acetate, propionate and butyrate concentration among the WGM treatments. Acetate/Propionate roatio of WGN treatments was higher than control after 12 h incubation(P<0.05). As a result of the artificial culture medium of wild-ginseng on rumen fermentation characteristics in vitro, microbial protein synthesis of WGM treatment was higher than control, and WGM 3% was the highest in all treatments(P<0.05). The effect of saponin in artificaial culture medium of wild-ginseng tended to decrease NH_(3)-N concentration, while it increases the microbial synthesis in early incubation. Therefor, artificial cultures medium of wild-ginseng ca increase utilization of feed by microbial and anti-protozoal effects of saponin, which may enhance microbial synthesis capacity in early fernentation period in rumen.
Hien, T.B.D.,Maeng, J.H.,Lee, B.H.,Seong, G.H.,Choo, J.,Lee, E.K. Elsevier Science Publishers 2012 Journal of biotechnology Vol.161 No.3
Phage display was performed against human IgG (hIgG) through five rounds of 'biopanning'. Each round consisted of: (1) incubating a library of phage-displayed 12-mer peptides sequences on hIgG-coated magnetic beads, (2) washing the unbound phages, and (3) eluting the bound phages. The eluted phages were either amplified to enrich the pool of positive clones or subjected to the next round without amplification. Through ELISA, four clones (F9, D1, G5, and A10) showing specific binding affinity to hIgG were identified. Among these, F9 had the highest affinity (K<SUB>d</SUB>=6.2nM), only one order of magnitude lower than the native anti-hIgG antibody (0.66nM). Following the DNA sequences of the selected clones, four 12-mer peptides were chemically synthesized. Among them, D1 peptide showed the highest binding affinity to hIgG via SPR biosensor measurements. This peptide was conjugated to biofunctionalized magnetic beads, and its immuno-binding ability was compared with that of the native antibody immobilized to magnetic beads. The mol-to-mol binding efficacy of the peptide-coated magnetic beads was approximately 1000-fold lower than that of the antibody-coated magnetic beads. Our results suggest a feasibility of using antibody-mimicking peptides identified by phage display technique for immuno-magnetic separation of an antigen.