Ischemic postconditioning protects cardiomyocytes against ischemia/reperfusion injury by inducing MIP2

Hong-Lin Zhu,Kang-Kai Wang,Xing Wei,Shun-Lin Qu,Chi Zhang,Xiao-Xia Zuo,Yan-Sheng Feng,Qi Luo,Guang-Wen Chen,Mei-Dong Liu,Lei Jiang,Xian-Zhong Xiao 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.8

Cardiomyocytes can resist ischemia/reperfusion (I/R)injury through ischemic postconditioning (IPoC)which is repetitive ischemia induced during the onset of reperfusion. Myocardial ischemic preconditioning up-regulated protein 2 (MIP2) is a member of the WD-40family proteins, we previously showed that MIP2 was up-regulated during ischemic preconditioning (IPC). As IPC and IPoC engaged similar molecular mechanisms in cardioprotection, this study aimed to elucidate whether MIP2 was up-regulated during IPoC and contributed to IPoC-mediated protection against I/R injury. The experiment was conducted on two models,an in vivo open chest rat coronary artery occlusion model and an in vitro model with H9c2 myogenic cells. In both models, 3 groups were constituted and randomly designated as the sham, I/R and IPoC/hypoxia postconditioning (HPoC) groups. In the IPoC group, after 45 min of ischemia, hearts were allowed three cycles of reperfusion/ischemia phases (each of 30 s duration)followed by reperfusion. In the HPoC group, after 6 h of hypoxia, H9c2 cells were subjected to three cycles of 10 minute reoxygenation and 10 minute hypoxia followed by reoxygenation. IPoC significantly reduced the infarct size, plasma level of Lactate dehydrogenase and creatine kinase MB in rats. 12 h after the reperfusion,MIP2 mRNA levels in the IPoC group were 10 folds that of the sham group and 1.4 folds that of the I/R group. Increased expression of MIP2 mRNA and attenuation of apoptosis were similarly observed in the HPoC group in the in vitro model. These effects were blunted by transfection with MIP2 siRNA in the H9c2cells. This study demonstrated that IPoC induced protection was associated with increased expression of MIP2. Both MIP2 overexpression and MIP2 suppression can influence the IPoC induced protection.

Formation of Magnetic Graphene Nanosheets for Rapid Enrichment and Separation of Methyl Orange from Water

Feng-Jun Zhang,Zhuo Zhang,Han Xuan,Hong-Chen Xia,Lei Zhu,오원춘,Fazhi Xie 한국세라믹학회 2014 한국세라믹학회지 Vol.51 No.6

Magnetic-graphene nanosheets have been synthesized via a simple effective chemical precipitation method followed by heattreatment. The composite nanosheets are super paramagnetic at room temperature and can be separated by an external magneticfield. The prepared magnetic-graphene nanosheets were characterized by X-ray diffraction (XRD), scanning electron microscopy(SEM), transmission electron microscopy (TEM), Raman spectroscopy, and BET surface area analysis. The resultsdemonstrated the successful attachment of iron oxide nanoparticles to graphene nanosheets. It was found that the attachednanoparticles were mainly Fe3O4. The magnetic-graphene nanosheets showed near complete methyl orange removal within 10mintues and would be practically usable for methyl orange separation from water.

Research on the spindle deflecting reliability of the wellbore trajectory control tool based on Palmgren-Miner

Hong Zhang,Lei Shi,Xuhui Liu,Xianshu Leng,Ding Feng,Paul Tu 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.12

Wellbore trajectory control tool is a sort of point-the-bit drilling tool to achieve the steering function while drilling. In complex conditions, the spindle bears the acting force of the bit pressure, the torque, the force of biasing mechanism and so on. So the force is very complex. Basing on the early research of our group, the further research of spindle deflecting reliability of prototype has been carried out. Based on the contact analysis between the tool spindle and the inner eccentric ring, the variation law of contact stress between the inner ring of the eccentric mechanism and the spindle is studied. Based on Palmgren-Miner and the fatigue life experiment of the spindle material, the cumulative damage calculation model of the spindle is established, and the fatigue life of spindle is solved. The result shows that the deflecting reliability of the spindle can be guaranteed.

Laser-processing of transparent solid materials for advanced photonic device applications

Feng Zhu,Rui Yang,Lei Wang,Yong-Sen Yu,Qi-Dai Chen,Hong-Bo Sun 한국생산제조학회 2012 한국생산제조시스템학회 학술발표대회 논문집 Vol.2012 No.10

We demonstrate the laser processing for fabrication of advanced photonic devices including fiber gratings and high transmittance biomimetic windows. The fiber gratings, including fiber Bragg gratings (FBGs) and long period fiber gratings (LPFGs), are fabricated in non-sensitized standard telecom single-mode fiber by femtosecond (fs) laser, making them hightemperature (1000 ℃) stable. The FBG written by fs laser combined with phase mask method is used for monitoring the thermal effect in fs laser interaction with silica glass. The peak temperature of the thermal impulse is estimated about 4800 ℃, which decays to around 500 ℃ in the pulse interval for irradiation of 1.1 mJ at repetition rate of 1 kHz. When the pulse energy and repetition rate increase in the fabrication process of FBGs, the fs-laser-induced grating regions extend from the fiber core to the cladding?air boundary due to the laser self-focusing. With the help of selective chemical etching of the laser modified grating regions, a microhole array structured FBG is formed, which is demonstrated in the application of simultaneous sensing for refractive index (RI) and temperature. For the fs laser direct writing of LPFGs, the period is 100 μm, making the total length of the LPFG only 5 mm. The LPFGs can couple the guided mode to high-order cladding modes of second-order diffraction, resulting in a high RI sensitivity of 5265 nm/RI unit in the RI range of 1.433 ∼ 1.443 and a low-temperature sensitivity of ?15.52 pm/℃ from 20 ℃ to 500 ℃. In addition, the high transmittance biomimetic windows are produced by direct laser interference ablation. The interfered laser beams are introduced into the surface of a bare optical substrate, where structured surfaces consisting of a micropillar array are produced by the multiple exposure of the two-beam interference ablation. As an example of the application, zinc sulfide substrates are processed with this technology.

Clenbuterol Inhibits SREBP-1c Expression by Activating CREB1

( Lei Zhou ),( Yi Xing Li ),( Tao Nie ),( Sheng Qiu Feng ),( Ji Hong Yuan ),( Hua Ping Chen ),( Zai Qing Yang ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.4

Formation of Magnetic Graphene Nanosheets for Rapid Enrichment and Separation of Methyl Orange from Water

Zhang, Feng-Jun,Zhang, Zhuo,Xie, Fa-Zhi,Xuan, Han,Xia, Hong-Chen,Zhu, Lei,Oh, Won-Chun The Korean Ceramic Society 2014 한국세라믹학회지 Vol.51 No.6

Magnetic-graphene nanosheets have been synthesized via a simple effective chemical precipitation method followed by heat treatment. The composite nanosheets are super paramagnetic at room temperature and can be separated by an external magnetic field. The prepared magnetic-graphene nanosheets were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, and BET surface area analysis. The results demonstrated the successful attachment of iron oxide nanoparticles to graphene nanosheets. It was found that the attached nanoparticles were mainly $Fe_3O_4$. The magnetic-graphene nanosheets showed near complete methyl orange removal within 10 mintues and would be practically usable for methyl orange separation from water.

Toxic Epidermal Necrolysis Induced by Sintilimab: A Case Report

Ya-lei Lye,Bin Shan,Chen-hong Jia,Jiang Liu,Juan Hou,Wen-li Du,Rui Feng,Ping Liang 대한피부과학회 2023 Annals of Dermatology Vol.35 No.-

Sintilimab is an anti-programmed cell death receptor-1 antibody. The phase III clinical trial ORIENT-12 confirmed the safety of sintilimab combined with pemetrexed/platinum in the treatment of advanced squamous non-small cell lung cancer. Skin reactions are the most commonly reported adverse events of immune checkpoint inhibitors and are rarely severe. We describe a case of toxic epidermal necrolysis related to sintilimab in an elderly oncologic patient. 3 weeks after immunotherapy, the patient developed an extensive rash and diffuse itching, rapidly evolving into macules, blisters, bullae and erosions. Causal evaluation was performed based on the algorithm of drug causality for epidermal necrolysis and national Food and Drug Administration qualitative analysis. The patient responded to high-dose glucocorticosteroid and supportive therapy, alongside with local wound care. If immune checkpoint inhibitors need to be extrapolated clinically, strictly following evidence-based research, promptly detecting and treating adverse reactions is crucial.

Expression and Effects of JMJD2A Histone Demethylase in Endometrial Carcinoma

Wang, Hong-Li,Liu, Mei-Mei,Ma, Xin,Fang, Lei,Zhang, Zong-Feng,Song, Tie-Fang,Gao, Jia-Yin,Kuang, Ye,Jiang, Jing,Li, Lin,Wang, Yang-Yang,Li, Pei-Ling Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.7

Previous studies have demonstrated that JMJD2A is a potential oncogene and is overexpressed in human tumors. However, its role in the endometrial carcinoma remains largely unknown. In this study, we discovered that JMJD2A was overexpressed in endometrial carcinoma, using immunohistochemistry, quantitative realtime polymerase chain reaction, and western blotting. Downregulation of JMJD2A led to reduced endometrial carcinoma RL95-2 and ISK cell proliferation, invasion and metastasis as asessed with cell counting kit-8, cell migration and invasive assays. Collectively, our results support that JMJD2A is a promoter of endometrial carcinoma cell proliferation and survival, and is a potential novel drug target.

Myeloid-Derived Suppressor Cells Are Associated with Viral Persistence and Downregulation of TCRζ Chain Expression on CD8+ T Cells in Chronic Hepatitis C Patients

Qing-Lei Zeng,Bin Yang,Hong-Qi Sun,Guo-Hua Feng,Lei Jin,Zheng-Sheng Zou,Zheng Zhang,Ji-Yuan Zhang,Fu-Sheng Wang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.1

Myeloid-derived suppressor cells (MDSCs) play an important role in impairing the function of T cells. We characterized MDSCs in two chronic hepatitis C (CHC) cohorts: a cross-sectional group that included 61 treatment-naive patients with CHC, 14 rapid virologic response (RVR) cases and 22 early virologic response (EVR) cases; and a longitudinal group of 13 cases of RVR and 10 cases of EVR after pegylated-interferon-α/ribavirin treatment for genotype 1b HCV infection. Liver samples from 32 CHC patients and six healthy controls were subjected to immunohistochemical analysis. MDSCs frequency in treatmentnaive CHC was significantly higher than in RVR, EVR, or healthy subjects and was positively correlated with HCV RNA. Patients infected with HCV genotype 2a had a significantly higher frequency of MDSCs than those infected with genotype 1b. Decreased T cell receptor (TCR) ζ expression on CD8+ T cells was significantly associated with an increased frequency of MDSCs in treatment-naive CHC patients and was restored by L-arginine treatment in vitro. Increased numbers of liver arginase-1+ cells were closely associated with the histological activity index in CHC. The TCR ζ chain was significantly downregulated on hepatic CD8+ T cells in CHC. During antiviral follow up, MDSCs frequency in peripheral blood mononuclear cells was directly correlated with the HCV RNA load in the plasma and inversely correlated with TCR ζ chain expression in CD8+ T cells in both RVR and EVR cases. Notably, the RVR group had a higher frequency of MDSCs at baseline than the EVR group. Collectively, this study provides evidence that MDSCs might be associated with HCV persistence and downregulation of CD8 ζ chain expression.

Ischemic postconditioning protects cardiomyocytes against ischemia/reperfusion injury by inducing MIP2

Zhu, Hong-Lin,Wei, Xing,Qu, Shun-Lin,Zhang, Chi,Zuo, Xiao-Xia,Feng, Yan-Sheng,Luo, Qi,Chen, Guang-Wen,Liu, Mei-Dong,Jiang, Lei,Xiao, Xian-Zhong,Wang, Kang-Kai Korean Society for Biochemistry and Molecular Bion 2011 Experimental and molecular medicine Vol.43 No.8

Cardiomyocytes can resist ischemia/reperfusion(I/R) injury through ischemic postconditioning (IPoC) which is repetitive ischemia induced during the onset of reperfusion. Myocardial ischemic preconditioning up-regulated protein 2 (MIP2) is a member of the WD-40 family proteins, we previously showed that MIP2 was up-regulated during ischemic preconditioning (IPC). As IPC and IPoC engaged similar molecular mechanisms in cardioprotection, this study aimed to elucidate whether MIP2 was up-regulated during IPoC and contributed to IPoC-mediated protection against I/R injury. The experiment was conducted on two models, an $in$ $vivo$ open chest rat coronary artery occlusion model and an $in$ $vitro$ model with H9c2 myogenic cells. In both models, 3 groups were constituted and randomly designated as the sham, I/R and IPoC/hypoxia postconditioning (HPoC) groups. In the IPoC group, after 45 min of ischemia, hearts were allowed three cycles of reperfusion/ischemia phases (each of 30 s duration) followed by reperfusion. In the HPoC group, after 6 h of hypoxia, H9c2 cells were subjected to three cycles of 10 minute reoxygenation and 10 minute hypoxia followed by reoxygenation. IPoC significantly reduced the infarct size, plasma level of Lactate dehydrogenase and creatine kinase MB in rats. 12 h after the reperfusion, MIP2 mRNA levels in the IPoC group were 10 folds that of the sham group and 1.4 folds that of the I/R group. Increased expression of MIP2 mRNA and attenuation of apoptosis were similarly observed in the HPoC group in the $in$ $vitro$ model. These effects were blunted by transfection with MIP2 siRNA in the H9c2 cells. This study demonstrated that IPoC induced protection was associated with increased expression of MIP2. Both MIP2 overexpression and MIP2 suppression can influence the IPoC induced protection.