GRAPHENE AS TUNABLE STATIONARY PHASE ADDITIVE FOR ENANTIOSEPARATION

FEI-YUE TU,LIN-YAN YU,JIN-GANG YU,XIAOQING CHEN,QIANG FU,FEI-PENG JIAO,ZHI-GUANG PENG,TING ZHANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.6

Graphene-based biosensor and chiral sensor have made significant progress in recent years. Because of the similarity of the optical enantiomers, enantioseparation is perhaps the most subtle to achieve. Developing more effectively chiral separation techniques based on graphene is highly desirable. Herein, pristine graphene was prepared and then applicated to assist resolution of two racemic drugs of propranolol and ofloxacin using thin-layer chromatography (TLC). In comparison with TLC chiral separation by only using pure D-(-)-tartaric acid as a selector with relatively low degree of separation, a mixture of graphene and D-(-)-tartaric acid is more attractive, efficient and ready available. The high specific resolution ability for racemic compounds aided by graphene might allow its potential application in future chiral separation technologies.

Dihydroartemisinine Enhances Dictamnine-induced Apoptosis via a Caspase Dependent Pathway in Human Lung Adenocarcinoma A549 Cells

An, Fu-Fei,Liu, Yuan-Chong,Zhang, Wei-Wei,Liang, Lei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Dictamnine (Dic) has the ability to exert cytotoxicity in human cervix, colon, and oral carcinoma cells and dihydroartemisinin (DHA) also has potent anticancer activity on various tumour cell lines. This report explores the molecular mechanisms by which Dic treatment and combination treatment with DHA and Dic cause apoptosis in human lung adenocarcinoma A549 cells. Dic treatment induced concentration- and time-dependent cell death. FCM analysis showed that Dic induced S phase cell cycle arrest at low concentration and cell apoptosis at high concentration in which loss of mitochondrial membrane potential (${\Delta}{\Psi}m$) was not involved. In addition, inhibition of caspase-3 using the specific inhibitor, z-DQMD-fmk, did not attenuate Dic-induced apoptosis, implying that Dic-induced caspase-3-independent apoptosis. Combination treatment with DHA and Dic dramatically increased the apoptotic cell death compared to Dic alone. Interestingly, pretreatment with z-DQMD-fmk significantly attenuated DHA and Dic co-induced apoptosis, implying that caspase-3 plays an important role in Dic and DHA co-induced cell apoptosis. Collectively, we found that Dic induced S phase cell cycle arrest at low concentration and cell apoptosis at high concentration in which mitochondria and caspase were not involved and DHA enhanced Dic induced A549 cell apoptosis via a caspase-dependent pathway.

Nested Case-control Study of Occupational Radiation Exposure and Breast and Esophagus Cancer Risk among Medical Diagnostic X Ray Workers in Jiangsu of China

Wang, Fu-Ru,Fang, Qiao-Qiao,Tang, Wei-Ming,Xu, Xiao-San,Mahapatra, Tanmay,Mahapatra, Sanchita,Liu, Yu-Fei,Yu, Ning-Le,Sun, Quan-Fu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

Medical diagnostic X-ray workers are one occupational group that expose to the long-term low-dose external radiation over their working lifetime, and they may under risk of different cancers. This study aims to determine the relationship between the occupational X-ray radiation exposure and cancer risk among these workers in Jiangsu, China. We conducted Nested case-control study to investigate the occupational X-ray radiation exposure and cancer risk. Data were collected through self-administered questionnaire, which includes but not limits to demographic data, personal behaviors and family history of cancer. Retrospective dose reconstruction was conducted to estimate the cumulative doses of the x-ray workers. Inferential statistics, t-test and 2 tests were used to compare the differences between each group. We used the logistic regression model to calculate the odds ratio (OR) and 95% confidence interval (CI) of cancer by adjusting the age, gender. All 34 breast cancer cases and 45 esophageal cancer cases that detected in a cohort conducted among health workers between 1950~2011 were included in this presented study, and 158 cancer-free controls were selected by frequency-matched (1:2). Our study found that the occupational radiation exposure was associated with a significantly increased cancer risk compared with the control, especially in breast cancer and esophageal cancer (adjusted OR=2.90, 95% CI: 1.19-7.04 for breast cancer; OR=4.19, 95% CI: 1.87-9.38 for esophageal cancer, and OR=3.43, 95% CI: 1.92-6.12 for total cancer, respectively). The occupational X-ray radiation exposure was associated with increasing cancer risk, which indicates that proper intervention and prevention strategies may be needed in order to bring down the occupational cancer risk.

Psychopathological Profile of Women with Breast Cancer Based on the Symptom Checklist-90-R

Pan, Xiong-Fei,Fei, Man-Dong,Zhang, Kenneth Y.,Fan, Zhen-Lie,Fu, Feng-Huan,Fan, Jin-Hu Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

Background: With effective early treatments, many breast cancer patients suffer from psychological distress due to adverse effects and lifelong physical disfigurement. Our study aimed to evaluate the psychopathological profile of breast cancer patients in comparison with healthy women and explored demographic correlates. Method: We consecutively enrolled breast cancer patients who came to the hospital for follow-up or rehabilitation care after primary treatment, and healthy female relatives or friends of inpatients in the Cancer Institute of Chinese Academy of Medical Sciences between August 30, 2010 and January 1, 2012. Psychopathological profile was assessed based on the Symptom Checklist-90-R (SCL-90-R) for patients and controls. We compared demographics such as age, ethnicity, education, marriage, and occupation, and incorporated these data plus cancer status for the association with the general SCL-90-R index and scores for 9 major symptom dimensions in multiple regression analysis. Results: We surveyed a total of 291 female breast cancer patients and 531 healthy women. The average age was $55.1{\pm}6.40$ years for breast cancer patients and $43.1{\pm}12.8$ for healthy controls (P<0.01). The mean survival was 5.20 years for cancer patients (range, 0.60-9.90 years). There were statistically significant differences in education, marriage, and occupation between the two groups (P<0.01). General index ($1.45{\pm}0.45$ versus $1.32{\pm}0.37$) and 8 dimension scores (excluding anxiety) on SCL-90-R were significantly higher in patients (P<0.05). Multiple regression analysis showed that the breast cancer status was positively correlated with general SCL-90-R index and 6 dimension scores (excluding the anxiety, phobic anxiety and paranoid ideation dimensions) (P<0.05). Regression coefficients ranged from 0.10 (depression) to 0.19 (somatization). Higher interpersonal sensitivity was noticed in single women compared to married women. Conclusions: Chinese patients with breast cancer demonstrate greater psychopathology compared to healthy controls. The breast cancer status is an independent contributing factor to the general psychopathological profile. Breast cancer patients should be given particular counseling and care to alleviate their psychological distress.

Variations of microbial community in Aconitum carmichaeli Debx. rhizosphere soilin a short-term continuous cropping system

Fei Xia,Lina Wang,Jiayang Chen,Meng Fu,Guodong Wang,Yaping Yan,Langjun Cui 한국미생물학회 2021 The journal of microbiology Vol.59 No.5

Aconitum carmichaeli Debx. (Ranunculaceae) is a potential source of an important herbal drug named “Fuzi”, which is derived from the lateral root of the plant. Increased therapeutic usage resulted in the great demand for artificial cultivation of A. carmichaeli, however, the obstacles caused by continuous cropping is a serious problem. Continuous cropping has shown to affect the soil biological and non-biological factors. The current study attempted to discover the variations of microbial communities and soil properties in shortterm continuous cropping of A. carmichaeli. An experimental procedure with A. carmichaeli planted two years continuously was established. The variation of the soil microbial community, disease incidence, soil properties, and the correlation between soil microbe and disease incidence were investigated. The disease incidence increased during the continuous cropping of A. carmichaeli. The PCoA and LefSe results indicated that fungal communities in rhizosphere soil were altered during the short-term continuous croppingand the bacterial community was disturbed by the cultivation of A. carmichaeli, however, in the following two years of continuous cropping period, the soil bacterial community has not changed obviously. Proportions of some fungal and bacterial genera were varied significantly (p < 0.05), and some genera of microflora showed a significant correlation with adisease incidence of A. carmichaeli. Microorganisms contributing to community composition discrepancy were also elucidated. Continuous cropping of A. carmichaeli disturbed the rhizosphere soil microbial community and altered the soil chemical parameters and soil pH. These variations in soil may be related to the occurrence of plant diseases. The current study will not only provide theoretical and experimental evidence for the A. carmichaeli continuous cropping obstacles but will also contribute to A. carmichaeli agricultural production and soil improvement.

Clinical Value of Real Time Elastography in Patients with Unexplained Cervical Lymphadenopathy: Quantitative Evaluation

Fu, Ying,Shi, Yun-Fei,Yan, Kun,Wang, Yan-Jie,Yang, Wei,Feng, Guo-Shuang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Ultrasonography is non-invasive and can give useful clues in the diagnosis of cervical lymphadenopathy, However, differential diagnosis is difficult in some situations even combined with color Doppler imaging. The present study was conducted to evaluate the clinical value of real time elastography in patients with unexplained cervical lymphadenopathy using a quantitative method. From May 2011 to February 2012, 39 enlarged lymph nodes from 39 patients with unexplained cervical lymphadenopathy were assessed. All the patients were examined by both B-mode ultrasound, color Doppler flow imaging and elastography. The method of analyzing elasto-graphic data was the calculation of the 10 parametres ("mean", "sd", "area%", "com", "kur", "ske", "con", "ent", "idm", "asm") offered by the software integrated into the Hitachi system. The findings were then correlated with the definitive tissue diagnosis obtained by lymph node dissection or biopsy. Final histology revealed 10 cases of metastatic lymph nodes, 11 cases of lymphoma, 12 cases of tuberculosis and 6 cases of nonspecific lymphadenitis. The significant distinguishing features for conventional ultrasound were the maximum short diameter (p=0.007) and absent of echogenic hilum (p=0.0293). The diagnostic accuracy was 43.6% (17/39 cases) and there were 17 patients with equivocal diagnosis. For elastography, "mean" (p=0.003), "area%" (p=0.009), "kurt" (p=0.0291), "skew" (p=0.014) and "cont" (p=0.012) demonstrated significant differences between groups. With 9 of the 17 patients with previous equivocal diagnoses (52.9%) definite and correct diagnoses could be obtained. The diagnostic accuracy for conventional ultrasound combined elastography was 69.2% (27/39 cases). There were differences in the diagnostic sensitivity of the two methods (p=0.0224). Ultrasound combined with elastography demonstrated higher rates of conclusive and accurate diagnoses in patients with unexplained cervical lymphadenopathy than conventional ultrasound. The quantitative program showed good correlation with the pathology of different lymph node diseases.

Study and Optimization of Structural Parametrics of Oil Seal by Response Surface Method

Fu-Ying Zhang,Jian-Lei Chen,Tian-Tian Li,Yu-Fei Zhang 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.2

The effect of structural parameters such as contact width, low-angle, high-angle, interference and waist depth on the sealing performance of rotary lip seal was analyzed by finite element method. Based on the macroscopic pumping effect model of the rotary shaft lip seal and the pumping rate equation, the influence of structural parameters on pumping rate was calculated and analyzed. The structural and operational parameters of lip seal were optimized by the response surface method. 32 kinds of the model were made by compounding levels on each factor, and the response surface regression characteristics of each model are analyzed. The results show that low-angle, high-angle and interference are the main factors that influence significantly the seal performance of rotary shaft lip seal, and contact width, low-angle and high-angle are major influential factors for pumping effect. Therefore, these factors are considered as the most important factors for an optimum design of the rotary shaft lip seal. The optimal conditions are obtained, and the results are also verified by analytical methods.

Study of co-excited green emission of Tb3+, Ce3+ and Gd3+ in yttrium aluminum garnet

Fei Huang,Limin Dong,Hao Wang,Weimin Wang,Yucheng Wang,Zhengyi Fu 한양대학교 세라믹연구소 2009 Journal of Ceramic Processing Research Vol.10 No.6

YAG : Tb3+, Ce3+, Gd3+ nano-phosphors derived from a sol-gel chemistry have been successfully synthesized and characterized by TG/DTA, XRD, SEM, and spectrometer. The results show that the phosphors have a uniform particle size distribution ranging 30-50 nm. The value of the lattice constant increased with an increase of the Gd3+ content. Tb3+ →Ce3+, Ce3+ →Tb3+ and Gd3+ →Ce3+ energy transfer existed in the co-excited system. The luminescence intensity was controlled by the concentration and ratio of the co-dopants. A small quantity of Gd3+ and Ce3+ evidently increased the green emission (5D4→7F5) of Tb3+. YAG : Tb3+, Ce3+, Gd3+ nano-phosphors derived from a sol-gel chemistry have been successfully synthesized and characterized by TG/DTA, XRD, SEM, and spectrometer. The results show that the phosphors have a uniform particle size distribution ranging 30-50 nm. The value of the lattice constant increased with an increase of the Gd3+ content. Tb3+ →Ce3+, Ce3+ →Tb3+ and Gd3+ →Ce3+ energy transfer existed in the co-excited system. The luminescence intensity was controlled by the concentration and ratio of the co-dopants. A small quantity of Gd3+ and Ce3+ evidently increased the green emission (5D4→7F5) of Tb3+.

Knowledge, Perceptions and Acceptability of HPV Vaccination among Medical Students in Chongqing, China

Fu, Chun-Jing,Pan, Xiong-Fei,Zhao, Zhi-Mei,Saheb-Kashaf, Michael,Chen, Feng,Wen, Ying,Yang, Chun-Xia,Zhong, Xiao-Ni Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Objectives: To evaluate medical students' knowledge of HPV and HPV related diseases and assess their attitudes towards HPV vaccination. Methods: A total of 605 medical undergraduates from Chongqing Medical University in China were surveyed using a structured and pretested questionnaire on HPV related knowledge. Results: Some 68.9% of the medical students were females, and mean age was 21.6 (${\pm}1.00$) years. Only 10.6% correctly answered more than 11 out of 14 questions on HPV related knowledge, 71.8% being willing to receive/advise on HPV vaccination. Female students (OR: 2.69; 95% CI: 1.53-4.72) and students desiring more HPV education (OR: 4.24; 95% CI: 1.67-10.8) were more willing to accept HPV vaccination. HPV vaccination acceptability was observed to show a positive association with HPV related knowledge. Conclusions: Our survey found low levels of HPV related knowledge and HPV vaccination acceptability among participating medical students. HPV education should be systematically incorporated into medical education to increase awareness of HPV vaccination.

Pose Measuring and Aligning of a Micro Glass Tube and a Hole on the Micro Sphere

Fu-Dong Li,De Xu,Zheng-Tao Zhang,Ya-Li Shi,Fei Shen 한국정밀공학회 2014 International Journal of Precision Engineering and Vol. No.

To ensure high uniformity of the inner space of the hollow sphere after assembly, the glass tube must be inserted into the hole on thesphere towards the center of the sphere. This paper deals with the pose measuring of the hole on the sphere and the glass tube andpose aligning of the two objects. The sphere is 500 mm in diameter with a 20 mm hole on it, and the glass tube is 17 mm in diameter. Novel pose measuring method for the hole on the sphere and the glass tube is developed, using two microscopic cameras, each oneacquires a projection vector of the object to be measured. A plane containing the optical axis of the microscopic camera and the posevector of the object is determined, with the microscopic camera calibrated in advance. Pose vector of the object to be measured canbe calculated by the intersection of the two planes acquired by the two microscopic cameras. Error analysis of the pose measuringmethod is conducted and experimental results were consistent with analytical results. Less than 0.7o pose aligning error is achievedusing the proposed pose measuring method and pose aligning method.