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Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae
( Fang-jie Yao ),( Li-xin Lu ),( Peng Wang ),( Ming Fang ),( You-min Zhang ),( Ying Chen ),( Wei-tong Zhang ),( Xiang-hui Kong ),( Jia Lu ),( Yoichi Honda ) 한국균학회 2018 Mycobiology Vol.46 No.1
The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker “SCL-18” consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster- type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.
( Chun Fang ),( Tong Cao ),( Ying Shan ),( Ye Xia ),( Yong Ping Xin ),( Chang Yong Cheng ),( Houhui Song ),( John Bowman ),( Xiao Liang Li ),( Xiang Yang Zhou ),( Wei Huan Fang ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.1
Listeria monocytogenes is a foodborne pathogen of considerable genetic diversity with varying pathogenicity. Initially, we found that the strain M7 was far less pathogenic than the strain Lm850658 though both are serovar 4a strains belonging to the lineage III. Comparative genomic approaches were then attempted to decipher the genetic basis that might govern the strain-dependent pathotypes. There are 2,761 coding sequences of 100% nucleotide identity between the two strains, accounting for 95.7% of the total genes in Lm850658 and 92.7% in M7. Lm850658 contains 33 specific genes, including a novel 20K prophage whereas strain M7 has 130 specific genes, including two large prophages (38K and 44K). To examine the roles of these specific prophages in pathogenicity, the 20K and 38K prophages were deleted from their respective strains. There were virtually no differences of pathogenicity between the deletion mutants and their parent strains, although some putative virulent factors like VirB4 are present in the 20K region or holin-lysin in the 38K region. In silico PCR analysis of 29 listeria genomes show that only strain SLCC2540 has the same 18 bp integration hotspot as Lm850658, whereas the sequence identity of their 20K prophages is very low (21.3%). The 38K and 44K prophages are located in two other different hotspots and are conserved in low virulent strains M7, HCC23, and L99. In conclusion, the 20K and 38K prophages of L. monocytogenes serovar 4a strains Lm850658 and M7 are not related to virulence but contribute to genetic diversity.
Tong Wei,Qian Fang 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.5
Polyhydroxyalkanoate (PHA), produced by mixed microbial cultures (MMCs), is a biodegradable biopolyester that alleviates the global plastic crisis in the nearest future. Even PHA has become a research hotspot, reviews on PHA composition determining thermodynamic and processing properties of PHA products are rare. The primary focus of the evaluation relies on the factors affecting the PHA monomer composition of MMC-PHA production. Besides, since the volatile fatty acids (VFAs) composition of the substrates strongly influences the PHA monomer composition, regulating the ratio of even-carbon to odd-carbon VFAs, PHA, vary properties could be obtained predictably. So oriented acid production process, the first stage process in three-stage MMC-PHA production that can regulate the VFAs composition, is also comprehensively introduced to help understand PHA monomer regulation.
Tong-Yul Cho,Jae-Hong Yoon,Yun-Kon Joo,Shihong-Zhang,Wei Fang,권식철,천희곤,Ming-Xi Li 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.54 No.3
High-velocity oxygen-fuel (HVOF) thermal-spray coating (coating) of micron-sized WC-CrC-Ni was carried out on an Inconel718 (In718) surface to improve the surface properties, friction and wear behaviors. During the spraying, binder metals were melted and a small portion of metal carbides, such as WC, Cr7C3 and Ni3C, were melted, partially melted, or decomposed to W2C, Cr, Ni and free carbon. A porous coating was formed by the evolution of carbon oxide gases formed by the reaction of the free carbon and the sprayed oxygen gas. For further improvement, a CO2 laser heat treatment (LH) was performed on the coating. Laser beam (10.6 μm, continuous mode, 400 W) irradiated an oval-shaped focal spot (5 mm × 4 mm) for 0.6 s at a scanning speed 400 mm/min, heating to about 950 - 1200℃ from the edge to the center of the spot. Laser tracks overlap each other 30 %. The porous coating was compacted by the LH, reducing the coating thickness by about 29 % from 280 μm to 200μm and the porosity by about 7 times from 2.6 ± 0.4 % to 0.35±0.06 %. The surface hardness of In718 (410±30 Hv) was increased more than twice to 983±101 Hv by the coating; furthermore, it was increased 45 % (1425±94 Hv) by LH for 0.6 s. The friction coefficient of the In718 surface (0.45±0.08) was reduced 29 % (0.32±0.02) and 9 % (0.29±0.03) by the coating and by the LH, respectively. The wear depth was reduced from 52μm to 30μm and to 12μm by the coating and by the LH, respectively. A HVOF coating of WC-CrC-Ni powder on a metal surface and a LH of the coating are highly recommended for improving the surface properties, the friction behavior and wear resistance.
Fang Gong,Tong Zhang,Jun Sheng,Jing Li,Xianghong Wang,Zhenming Chi 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.5
The extracellular inulinase of the marine yeast Pichia guilliermondii strain 1 was purified to homogeneity resulting in a 7.2-fold increase in specific inulinase activity. The molecular mass of the purified enzyme was estimated to be 50.0 kDa. The op-timal pH and temperature for the purified enzyme were 6.0 and 60C, respectively. The enzyme was activated by Mn²+, Ca²+, K+, Li+, Na+, Fe³+, Fe²+, Cu²+, and Co²+, but Mg²+, Hg²+, and Ag+ inhibited activity. The enzyme was strongly inhibited by phenylmethanesulphonyl fluoride (PMSF), iodoacetic acid, EDTA, and 1, 10-phenanthroline. The Km and Vmax values of the purified inulinase for inulin were 21.1 mg/mL and 0.08 mg/min, respectively. A large number of monosaccharides were de-tected after the hydrolysis of inulin. The deduced protein sequence from the cloned P. guilliermondii strain 1 inulinase gene contained the consensus motifs R-D-P-K-V-F-W-H and W-M-N-D-P-N-G, which are conserved among the inulinases from other microorganisms.
Toxicogenomics and Cell-based Assays for Toxicology
Tong, Weida,Fang, Hong,Mendrick, Donna Korean Society for Bioinformatics 2009 Interdisciplinary Bio Central (IBC) Vol.1 No.3
Toxicity is usually investigated using a set of standardized animal-based studies which, unfortunately, fail to detect all compounds that induce human adverse events and do not provide detailed mechanistic information of observed toxicity. As an alternative to conventional toxicology, toxicogenomics takes advantage of currently advanced technologies in genomics, proteomics, metabolomics, and bioinformatics to gain a molecular level understanding of toxicity and to enhance the predictive power of toxicity testing in drug development and risk/safety assessment. In addition, there has been a renewed interest, particularly in various government agencies, to prioritize and/or supplement animal testing with a battery of mechanistically informative in vitro assays. This article provides a brief summary of the issues, challenges and lessons learned in these fields and discuss the ways forward to further advance toxicology using these technologies.