Optimization and Molecular Characterization of Exoelectrogenic Isolates for Enhanced Microbial Fuel Cell Performance

Nwagu, Kingsley Ekene,Ekpo, Imo A.,Ekaluo, Benjamin Utip,Ubi, Godwin Michael,Elemba, Munachimso Odinakachi,Victor, Uzoh Chukwuma The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.4

In this study we attempted to screen bacteria and fungi that generate electricity while treating wastewater using optimized double-chamber microbial fuel cell (MFC) system parameters. Optimization was carried out for five best exoelectrogenic isolates (two bacteria and three fungi) at pH values of 6.0, 7.5, 8.5, and 9.5, and temperatures of 30, 35, 40, and 45℃; the generated power densities were measured using a digital multimeter (DT9205A). The isolates were identified using molecular characterization, followed by the phylogenetic analysis of isolates with known exoelectrogenic microorganisms. The bacterium, Proteus species, N6 (KX548358.1) and fungus, Candida parapsilosis, S10 (KX548360) produced the highest power densities of 1.59 and 1.55 W/m² (at a pH of 8.5 and temperatures of 35 and 40℃) within 24 h, respectively. Other fungi-Clavispora lusitaniae, S9 (KX548359.1) at 40℃, Clavispora lusitaniae, S14 (KX548361.1) at 35℃-and bacterium-Providencia species, N4 (KX548357.1) at 40℃-produced power densities of 1.51, 1.46, and 1.44 W/m², respectively within 24 h. The MFCs achieved higher power densities at a pH of 8.5, temperature of 40℃ within 24 h. The bacterial isolates have a close evolutionary relationship with other known exoelectrogenic microorganisms. These findings helped us determine the optimal pH, temperature, evolutionary relationship, and exoelectrogenic fungal species other than bacteria that enhance MFC performance.

Evaluation of In Vivo Antimalarial Activities of Ethanolic Leaf and Seed Extracts of Telfairia occidentalis

Okokon, Jude E.,Ekpo, Arit J.,Eseyin, Olorunfemi A. The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.3

The leaves and seeds of Telfairia occidentalis are used as vegetables in making soups in Southern Nigeria. In this study, we investigated the antimalarial activity of leaf and seed extracts in vivo in mice infected with Plasmodium berghei berghei during early and established infections. T. occidentalis leaf extract (250-750 mg/kg/day) exhibited antiplasmodial activity both in the 4-day early infection test and in established infection with a marked increase of the mean survival time, which, however, remained lower than that achieved with the standard drug, chloroquine (5 mg/kg/day). The seed extract (450-1,350 mg/kg/day) also demonstrated a promising blood schizontocidal activity in early and established infections. This plant possesses significant antiplasmodial activities, which may be exploited in the control of malaria.

Optimization and Molecular Characterization of Exoelectrogenic Isolates for Enhanced Microbial Fuel Cell Performance

Kingsley Ekene Nwagu1,Imo A. Ekpo,Benjamin Utip Ekaluo,Godwin Michael Ubi,Munachimso Odinakachi Elemba,Uzoh Chukwuma Victor 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.4

In this study we attempted to screen bacteria and fungi that generate electricity while treating wastewater using optimized double-chamber microbial fuel cell (MFC) system parameters. Optimization was carried out for five best exoelectrogenic isolates (two bacteria and three fungi) at pH values of 6.0, 7.5, 8.5, and 9.5, and temperatures of 30, 35, 40, and 45℃; the generated power densities were measured using a digital multimeter (DT9205A). The isolates were identified using molecular characterization, followed by the phylogenetic analysis of isolates with known exoelectrogenic microorganisms. The bacterium, Proteus species, N6 (KX548358.1) and fungus, Candida parapsilosis, S10 (KX548360) produced the highest power densities of 1.59 and 1.55 W/m2 (at a pH of 8.5 and temperatures of 35 and 40℃) within 24 h, respectively. Other fungi— Clavispora lusitaniae, S9 (KX548359.1) at 40℃, Clavispora lusitaniae, S14 (KX548361.1) at 35℃—and bacterium— Providencia species, N4 (KX548357.1) at 40℃—produced power densities of 1.51, 1.46, and 1.44 W/m2, respectively within 24 h. The MFCs achieved higher power densities at a pH of 8.5, temperature of 40℃ within 24 h. The bacterial isolates have a close evolutionary relationship with other known exoelectrogenic microorganisms. These findings helped us determine the optimal pH, temperature, evolutionary relationship, and exoelectrogenic fungal species other than bacteria that enhance MFC performance.

Optimization and Molecular Characterization of Exoelectrogenic Isolates for Enhanced Microbial Fuel Cell Performance

( Kingsley Ekene Nwagu ),( Imo A. Ekpo ),( Benjamin Utip Ekaluo ),( Godwin Michael Ubi ),( Munachimso Odinakachi Elemba ),( Uzoh Chukwuma Victor ) 한국미생물 · 생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.4

In this study we attempted to screen bacteria and fungi that generate electricity while treating wastewater using optimized double-chamber microbial fuel cell (MFC) system parameters. Optimization was carried out for five best exoelectrogenic isolates (two bacteria and three fungi) at pH values of 6.0, 7.5, 8.5, and 9.5, and temperatures of 30, 35, 40, and 45℃; the generated power densities were measured using a digital multimeter (DT9205A). The isolates were identified using molecular characterization, followed by the phylogenetic analysis of isolates with known exoelectrogenic microorganisms. The bacterium, Proteus species, N6 (KX548358.1) and fungus, Candida parapsilosis, S10 (KX548360) produced the highest power densities of 1.59 and 1.55 W/m² (at a pH of 8.5 and temperatures of 35 and 40℃) within 24 h, respectively. Other fungi― Clavispora lusitaniae, S9 (KX548359.1) at 40℃, Clavispora lusitaniae, S14 (KX548361.1) at 35℃―and bacterium― Providencia species, N4 (KX548357.1) at 40℃―produced power densities of 1.51, 1.46, and 1.44 W/m², respectively within 24 h. The MFCs achieved higher power densities at a pH of 8.5, temperature of 40℃ within 24 h. The bacterial isolates have a close evolutionary relationship with other known exoelectrogenic microorganisms. These findings helped us determine the optimal pH, temperature, evolutionary relationship, and exoelectrogenic fungal species other than bacteria that enhance MFC performance.

Evaluation of In Vivo Antimalarial Activities of Ethanolic Leaf and Seed Extracts of Telfairia occidentalis

Jude E. Okokon,Arit J. Ekpo,Olorunfemi A. Eseyin 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.3

The leaves and seeds of Telfairia occidentalis are used as vegetables in making soups in Southern Nigeria. In this study, we investigated the antimalarial activity of leaf and seed extracts in vivo in mice infected with Plasmodium berghei berghei during early and established infections. T. occidentalis leaf extract (250–750mg/kg/day) exhibited antiplasmodial activity both in the 4-day early infection test and in established infection with a marked increase of the mean survival time, which, however, remained lower than that achieved with the standard drug, chloroquine (5mg/kg/day). The seed extract (450–1,350mg/kg/day) also demonstrated a promising blood schizontocidal activity in early and established infections. This plant possesses significant antiplasmodial activities, which may be exploited in the control of malaria.

Coaching Intervention Indices as Predictors of Effective Coaching among Nigerian Coaches

O. A. Adegbesan,A.S Chidi,O. M. Jaiyeoba,S. Mohammed,G. U. A. Ekpo 한국코칭능력개발원 2014 International Journal of Coaching Science Vol.8 No.2

The purpose of this research was the investigation of coaching intervention behavior as predictors of effective coaching. We adopted a descriptive research design of correlation type to gain an in-depth understanding of the participants’ views on the coaching intervention and effective coaching practice variables. The participants used for this study were 50 male and 34 female athletes with mean age (mean = 24.52; SD 9.4). Their sport involvement is athletics, judo, swimming, volleyball, handball and table tennis. The measures used were Perceived Coaching Intervention Questionnaire (r=.84) and Effective Coaching Scale (r=.79). The results shows positive correlations between Effective Coaching and Encouragement (r=.514**, P<.05), Technical Information (r=.614**, P< .05), Negative Feedback (r=.591**, P<.05) Positive feedback (r=.585**, P<.05). 46.9% of the variance in the dependent variables was explained by four predictor variables when taken together. Conclusively, it was identified that positive feedback was the most potent of the four interventions as perceived by the athletes. The recognition of these excellent intervention coaching practice along the various sporting pathway may supports developments in models and frameworks for sport in Nigeria and globally.