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Construction and Commissioning of the KSTAR Current Feeder System
Park, Y.M.,Lee, Y.J.,Yaung-Soo Kim,Woo, I.S.,Kwag, S.W.,Chang, Y.B.,Song, N.H.,Park, H.T.,Kim, C.S.,Lee, K.S.,Bang, E.N.,Chu, Y.,Yonekawa, H.,Park, K.R.,Yang, H.L.,Ha, T.H.,Bak, J.S. IEEE 2009 IEEE transactions on applied superconductivity Vol.19 No.3
<P>The function of the current feeder system (CFS) is for conducting large currents from the power supplies to the KSTAR superconducting (SC) magnets. The CFS consists of SC bus-lines, joints, and current leads. The bus-line conductor is a circular cable-in-conduit conductor (CICC), which consists of a 4.5 mm thick stainless steel 316L seamless pipe containing 324 strands of chrome coated NbTi superconductor and 243 strands of OFHC. The ends of the CICC are assembled with specially designed lap joints. The joining resistance is controlled to less than 2.5 nano-ohm to minimize Joule heating. The outer surfaces of the CICC were electrically insulated up to 15 kV with jackets made of Kapton film and prepreged E-glass tape. Helically wrapped conducting fiber was used to measure the voltages of bus-line quenches. Two pairs of prototype brass leads for poloidal field (PF) and toroidal field (TF) coils have been fabricated and tested up to the currents of 26 kA for the PF leads and 35 kA for the TF leads. The test results satisfied all the requirements so that all 18 leads were manufactured and assembled on site. This paper will describe the detailed manufacturing progress and commissioning results of the KSTAR CFS.</P>
A Novel Quasi-Resonant ZCS-PFM DC-DC Switching Regulator with Loosely-Coupled Flyback Inductors
E.H.Chu,S.Chandhaket,S.Moisseev,E.Hiraki,M.Nakaoka,H.Kifune 전력전자학회 2001 ICPE(ISPE)논문집 Vol.2001 No.10
This paper presents a novel topological prototype of voltage source series quasi-resonant zero current soft-switching pulse frequency modulated dc-dc power converter circuit using IGBTs which incorporates a high-frequency flyback transformer link. Its steady-state operating principle is described on the basis of simulation analysis, along with the open loop controlled power regulation characteristics of the multi-functional coupled inductors linked dc-dc power converter operating under a principle of zero current soft switching commutation.<br/>
Expression analysis of an elastin-like polypeptide (ELP) in a cell-free protein synthesis system
Chu, H.S.,Lee, K.H.,Park, J.E.,Kim, D.M.,Kim, B.G.,Won, J.I. IPC Science and Technology Press ; Elsevier Scienc 2010 Enzyme and microbial technology Vol.46 No.2
An elastin-like polypeptide (ELP) fusion protein was expressed in a cell-free protein synthesis system, and the expression profile was analyzed quantitatively. By selective addition of specific amino acids constituting ELP molecules, the expression level of the ELP fusion protein was improved by 1.3-1.8 times as high as positive control. This result implies that the expression amount of long repetitive polypeptides, which was dramatically decreased in vivo system, can be compensated to a degree by adding repeatedly consumed amino acids in vitro system. Presented results demonstrate the potential of cell-free protein synthesis for high-throughput study of various repetitive polypeptides.
Oh, E-T,Park, M-T,Song, M-J,Lee, H,Cho, Y U,Kim, S J,Chu, Y-C,Choi, E K,Park, H J Macmillan Publishers Limited 2014 Oncogene Vol.33 No.10
Despite strong possibility that endothelial cells (ECs) of tumors and normal tissues may differ in various aspects, most previous studies on ECs have used normal cells. Here, we purified ECs from tumorous and normal human breast tissues, and studied the effect of radiation on angiogenesis and relevant molecular mechanisms in these cells. We found that in normal tissue-derived ECs (NECs), 4 Gy irradiation increased tube formation, matrix metalloproteinase 2 (MMP-2) expression and extracellular signal-regulated kinase (ERK) pathway activation. In cancer-derived ECs (CECs), however, 4 Gy irradiation significantly reduced tube formation, increased the production of angiostatin and interleukin-6 (IL-6), and upregulated AKT and c-Jun N-terminal kinase (JNK) pathway activation. Knockdown experiments showed that siMMP-2 efficiently inhibited tube formation by irradiated NECs, whereas siPlasminogen effectively attenuated the radiation-induced suppression of tube formation and the upregulation of angiostatin in CECs. Moreover, siIL-6 clearly inhibited the radiation-induced generation of angiostatin in CECs. Inhibition of ERK with a pharmacological inhibitor or small interfering RNAs (siRNAs) markedly suppressed the radiation-induced tube formation and MMP-2 upregulation in NECs, whereas the inhibition of either AKT or JNK with pharmacological inhibitor or siRNA treatment of CECs markedly attenuated the inhibition of tube formation and the upregulation of angiostatin and IL-6 caused by 4 Gy irradiation. These observations collectively demonstrate that there are distinct differences in the radiation responses of NECs and CECs, and might provide important clues for improving the efficacy of radiation therapy.
Kim, H. S.,Chu, Y. J.,Park, C. H.,Lee, E. Y.,Kim, H. S. Springer Science + Business Media 2015 Marine biotechnology Vol.17 No.6
<P>A novel oligoalginate lyase from a marine bacterium, Sphingomonas sp. strain MJ-3, exhibited a unique alginate degradation activity that completely depolymerizes alginate to monomers through the formation of oligomers. In order to reveal the reason why MJ-3 oligoalginate can exhibit both endolytic and exolytic alginate lyase activities, ten mutants were developed and characterized on the basis of homology modeling. When the recombinant cell lysates containing the mutated proteins of MJ-3 oligoalginate lyase were allowed to react with alginate, the Asn177Ala, His178Ala, Tyr234Phe, His389Ala, and Tyr426Phe mutants showed reduced oligoalginate lyase activity, whereas the Arg236Ala mutant exhibited endolytic activity. Interestingly, the overexpressed Arg236Ala protein (79.6 kDa) was proteolytically cleaved into two fragments, i.e., the N-terminal 32.0-kDa and the C-terminal 47.6-kDa fragments. Both the purified N-terminal and C-terminal fragments showed endolytic lyase activity. They preferentially degraded a heteropolymeric (polyMG) block than poly-beta-D-mannuronate (polyM) or poly-alpha-L-guluronate (polyG) blocks. These results suggest that the oligoalginate lyase activity of MJ-3 enzyme is derived from the cooperative interaction between the N- and C-terminal endolytic alginate lyase domains in the intact enzyme.</P>