Acute MPTP treatment impairs dendritic spine density in the mouse hippocampus

Poornima D. E. Weerasinghe-Mud,Mary Jasmin Ang,Mai Wada,Sung-Ho Kim,Taekyun Shin,Miyoung Yang,Changjong Moon 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Among the animal models of Parkinson"s disease (PD), the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-lesioned mouse model has shown both dopaminergic (DA) damage and related motor control defects, as observed in patients with PD. Recent studies have suggested that the DA system interacts with the synaptic plasticity of the hippocampus in PD. However, little is known about how alterations in the hippocampal structural plasticity are affected by the DA damage in MPTP-lesioned models. In the present study, we investigated alterations in dendritic complexity and spine density in the mouse hippocampus following acute MPTP treatment (22 mg/kg, i.p., four times/day, 2-h intervals). We confirmed that acute MPTP treatment significantly decreased initial motor function and reduced persistently the number of tyrosine hydroxylase-positive DA neurons in the substantia nigra. Golgi staining showed that acute MPTP treatment significantly reduced the spine density of neuronal dendrites in the cornu ammonis 1 (CA1) apical/basal and dentate gyrus (DG) subregions of the mouse hippocampus at 8 and 16 days after treatment, although it did not affect dendritic complexity (e.g., number of crossing dendrites, total dendritic length, and branch points per neuron) in both CA1 and DG subregions at all time points after treatment. Therefore, the present study provides anatomical evidence that acute MPTP treatment affects synaptic structure in the hippocampus during the late phase after acute MPTP treatment in mice, independent of any changes in the dendritic arborization of hippocampal neurons. These findings offer data for the ability of the acute MPTP-lesioned mouse model to replicate the non-nigrostriatal lesions of clinical PD.

AKARI mid-infrared slitless spectroscopic survey of star-forming galaxies at <i>z</i> ≲ 0.5

Ohyama, Y.,Wada, T.,Matsuhara, H.,Takagi, T.,Malkan, M.,Goto, T.,Egami, E.,Lee, H.-M.,Im, M.,Kim, J.H.,Pearson, C.,Inami, H.,Oyabu, S.,Usui, F.,Burgarella, D.,Mazyed, F.,Imanishi, M.,Jeong, W.-S.,Miya EDP Sciences 2018 Astronomy and astrophysics Vol.618 No.-

<P><I>Context.</I> Deep mid-infrared (MIR) surveys have revealed numerous strongly star-forming galaxies at redshift <I>z</I> ≲ 2. Their MIR fluxes are produced by a combination of continuum and polycyclic aromatic hydrocarbon (PAH) emission features. The PAH features can dominate the total MIR flux, but are difficult to measure without spectroscopy.</P><P><I>Aims.</I> We aim to study star-forming galaxies by using a blind spectroscopic survey at MIR wavelengths to understand evolution of their star formation rate (SFR) and specific SFR (SFR per stellar mass) up to <I>z</I> ≃ 0.5, by paying particular attention to their PAH properties.</P><P><I>Methods.</I> We conducted a low-resolution (<I>R</I> ≃ 50) slitless spectroscopic survey at 5-13 <I>μ</I>m of 9 <I>μ</I>m flux-selected sources (>0.3 mJy) around the north ecliptic pole with the infrared camera (IRC) onboard AKARI. After removing 11 AGN candidates by using the IRC photometry, we identify 48 PAH galaxies with PAH 6.2, 7.7, and 8.6 <I>μ</I>m features at <I>z</I> < 0.5. The rest-frame optical-MIR spectral energy distributions (SEDs) based on CFHT and IRC imaging covering 0.37-18 <I>μ</I>m were produced, and analysed in conjunction with the PAH spectroscopy. We defined the PAH enhancement by using the luminosity ratio of the 7.7 <I>μ</I>m PAH feature over the 3.5 <I>μ</I>m stellar component of the SEDs.</P><P><I>Results.</I> The rest-frame SEDs of all PAH galaxies have a universal shape with stellar and 7.7 <I>μ</I>m bumps, except that the PAH enhancement significantly varies as a function of the PAH luminosities. We identify a PAH-enhanced population at <I>z</I> ≳ 0.35, whose SEDs and luminosities are typical of luminous infrared galaxies. They show particularly larger PAH enhancement at high luminosity, implying that they are vigorous star-forming galaxies with elevated specific SFR. Our composite starburst model that combines a very young and optically very thick starburst with a very old population can successfully reproduce most of their SED characteristics, although we cannot confirm this optically think component from our spectral analysis.</P>

Impacts of oxygen sensitization methods on the deposition and microstructure of ternary lead chalcogenide alloys

Su Peter,Stoll Katherine E.,Agarwal Samarth,Maksimov Oleg,Bhattacharya Pijush,Bhandari Harish B.,Wada Kazumi,Kimerling Lionel C.,Agarwal Anuradha 한국물리학회 2022 Current Applied Physics Vol.36 No.-

Oxygen sensitization and incorporation of ternary lead chalcogenide PbSe1-xTex thin films was investigated with two methods: adding oxygen via PbO to the bulk source alloy and post-deposition oxygen annealing. Characterization of the composition, structure, and morphology of these films confirmed that they follow Vegard’s law for lattice parameter, and adding PbO to the source alloy did not impact the lattice parameter. However, adding PbO changed the electrical carrier properties observed in Hall effect measurements without forming any new oxide phase. Conversely, post-deposition annealing increased the lattice parameter due to oxygen incorporation into the lattice via interstitials in samples with appropriate grain boundary orientations. Morphological analysis revealed that PbSe0.8Te0.2 films demonstrated (100) texture, while PbSe0.6Te0.4 films demonstrated (111) texture with resulting grain boundary orientations more favorable to oxygen diffusion and incorporation. This varying oxygen incorporation from PbO source and oxygen annealing methods reveals trends that can lead to improved photodetector performance.

An experimental study of tip-vortex structures behind a small wind turbine with a flanged diffuser

Abe, K.,Kihara, H.,Sakurai, A.,Wada, E.,Sato, K.,Nishida, M.,Ohya, Y. Techno-Press 2006 Wind and Structures, An International Journal (WAS Vol.9 No.5

An experimental study of tip-vortex structures behind a small wind turbine with a flanged diffuser

K. Abe,H. Kihara,A. Sakurai,E. Wada,K. Sato,M. Nishida,Y. Ohya 한국풍공학회 2006 Wind and Structures, An International Journal (WAS Vol.9 No.5

700-nm Zwitterionic Near-Infrared Fluorophores for Dual-Channel Image-Guided Surgery

Hyun, H.,Henary, M.,Gao, T.,Narayana, L.,Owens, E. A.,Lee, J. H.,Park, G.,Wada, H.,Ashitate, Y.,Frangioni, J. V. Elsevier Science B.V., Amsterdam. 2016 Molecular imaging and biology Vol.18 No.1

<P>Purpose: The purpose of this study was to develop a family of 700-nm zwitterionic pentamethine indocyanine near-infrared fluorophores that would permit dual-channel image-guided surgery. Procedures: Three complementary synthetic schemes were used to produce novel zwitterionic chemical structures. Physicochemical, optical, biodistribution, and clearance properties were compared to Cy5.5, a conventional pentamethine indocyanine now used for biomedical imaging. Results: ZW700-1a, ZW700-1b, and ZW700-1c were synthesized, purified, and analyzed extensively in vitro and in vivo. All molecules had extinction coefficients >= 199,000 M-1 cm(-1), emission >= 660 nm, and stability >= 99 % after 24 h in warm serum. In mice, rats, and pigs, >= 80 % of the injected dose was completely eliminated from the body via renal clearance within 4 h. Either alone or conjugated to a tumor targeting ligand, ZW700-1a permitted dual-channel, high SBR, and simultaneous imaging with 800-nm NIR fluorophores using the FLARE (R) imaging system. Conclusions: Novel 700-nm zwitterionic NIR fluorophores enable dual-NIR image-guided surgery.</P>

Amla (Emblica officinalis Gaertn.) Extracts Reduce Oxidative Stress in Streptozotocin-Induced Diabetic Rats

T.P. Rao,N. Sakaguchi,L.R. Juneja,E. Wada,T. Yokozawa 한국식품영양과학회 2005 Journal of medicinal food Vol.8 No.3

The antioxidant properties of amla extracts and their effects on the oxidative stress in streptozotocin-induceddiabetes were examined in rats. Amla in the form of either the commercial enzymatic extract SunAmla (Taiyo Kagaku Co.Ltd., Yokkaichi, Japan) (20 or 40 mg/kg of body weight/day) or a polyphenol-rich fraction of ethyl acetate extract (10 or 20mg/kg of body weight/day) was given orally for 20 days to the streptozotocin-induced diabetic rats. Amla extracts showedstrong free radical scavenging activity. Amla also showed strong inhibition of the production of advanced glycosylated endproducts. The oral administration of amla extracts to the diabetic rats slightly improved body weight gain and also signifi-cantly alleviated various oxidative stress indices of the serum of the diabetic rats. The elevated serum levels of 5-hydrox-ymethylfurfural, which is a glycosylated protein that is an indicator of oxidative stress, were significantly reduced dose-de-pendently in the diabetic rats fed amla. Similarly, the serum level of creatinine, yet another oxidative stress parameter, wasalso reduced. Furthermore, thiobarbituric acid-reactive substances levels were significantly reduced with amla, indicating areduction in lipid peroxidation. In addition, the decreased albumin levels in the diabetic rats were significantly improved withamla. Amla also significantly improved the serum adiponectin levels. These results form the scientific basis supporting theefficacy of amla for relieving the oxidative stress and improving glucose metabolism in diabetes.

The North Ecliptic Pole Wide survey of AKARI: a near- and mid-infrared source catalog

Kim, S. J.,Lee, H. M.,Matsuhara, H.,Wada, T.,Oyabu, S.,Im, M.,Jeon, Y.,Kang, E.,Ko, J.,Lee, M. G.,Takagi, T.,Pearson, C.,White, G. J.,Jeong, W.-S.,Serjeant, S.,Nakagawa, T.,Ohyama, Y.,Goto, T.,Takeuch EDP Sciences 2012 Astronomy and astrophysics Vol.548 No.-

Acupuncture Effect on Pain, Mouth Opening Limitation and on the Energy Meridians in Patients with Temporomandibular Dysfunction: A Randomized Controlled Trial

Vera LR. Zotelli,Cassia M. Grillo,Maria LB. Gil,Ronaldo S. Wada,Jorge E. Sato,Maria da Luz Rosario de Sousa 사단법인약침학회 2017 Journal of Acupuncture & Meridian Studies Vol.10 No.5

Temporomandibular disorders (TMD), recognized as the most common conditions of chronic orofacial pain, have a multifactorial etiology. Acupuncture can help to relieve the pain and discomfort associated with these conditions, because it can rebalance the energy (Qi) circulating in the meridians. The aim of the study was to verify the effectiveness of acupuncture in treating the pain; mouth opening limitation, and energy circulating in the meridians of patients with TMD of muscular or mixed origin. This was a controlled, randomized, double-blind clinical trial conducted at the Piracicaba Dental School (FOP/Unicamp), in Piracicaba SP, Brazil. The Treatment Group received acupuncture with real penetration of the needle, and the Placebo Group received a sham treatment without needle penetration. The acupoints used were: ST6, ST7, SI18, GV20, GB20, BL10, and LI4, during treatment performed for four weekly sessions. The TMD and mouth opening were evaluated according to the Research Diagnostic Criteria for Temporomandibular Disorders (RDC). The measurements of the energy at the meridians were performed by the Ryodoraku method, before and after acupuncture in all of the sessions in both groups. The results showed no decrease in pain in the Treatment Group when compared with the Placebo Group (p Z 0.2261). There was no increase in the oral opening limit in the Treatment Group compared with the Placebo Group (p > 0.05). Regarding the energy levels, after acupuncture, there was a decrease in Yang energy in all sessions (p < 0.05), in both groups, however, only real acupuncture was effective in maintaining the Yin energy average throughout the four sessions, with significant difference between groups (p Z 0.0198). In conclusion, volunteers with TMD presented a pattern of energy deficiency and the most prevalent imbalance patterns identified were in the meridians coupled to the kidney and bladder, and in the Shao Yin (heart/kidney) and Shao Yang (triple energizer/gall bladder) energetic planes. The acupuncture points used were equally effective in reducing pain in both groups; increasing the unassisted mouth opening limitation without pain in the Treatment Group, and were also effective in preserving the Yin energy in the Treatment Group. The Yang energy decreased equally in both groups.

Patterns of Energy Imbalance of the Meridians in Patients with Temporomandibular Dysfunction

Vera L. Rasera Zotelli,Ca´ssia M. Grillo,Maria L. Bressiani Gil,Ronaldo S. Wada,Jorge E. Sato,Maria da Luz R. de Sousa 사단법인약침학회 2018 Journal of Acupuncture & Meridian Studies Vol.11 No.1

Temporomandibular dysfunction (TMD) is a set of changes that affects the muscles of mastication, temporomandibular joint, teeth, and associated periodontal and orofacial structures. According to Traditional Chinese Medicine, the imbalance of energy (Qi) circulating in the acupuncture meridians is always the primary etiologic cause of any physical manifestation. The aim of this study was to describe the patterns of Qi imbalance in patients with TMD by means of an objective measurement. The clinical study was conducted at the Piracicaba Dental School (FOP/Unicamp), in Piracicaba-SP, Brazil. We evaluated 40 adult volunteers with TMD. The Qi measurement was carried out by the researcher using the Ryodoraku method using 24 points representing the 12 acupuncture meridians: LU9 (Taiyuan), PC7 (Daling), HT7 (Shemen), SI5 (Yanggu), TE4 (Yangchi), LI5 (Yangxi), SP3 (Taibai), LR3 (Taichong), KI3 (Taixi), BL64 (Jinggu), GB40 (Qiuxu), and ST42 (Chongyang). The average total Qi of 40 volunteers (21.7 μA ± 1.5), was below the normal range (40–60 μA) and was classified as deficiency of Qi (empty). The coupled meridians that showed the highest Qi imbalance were the kidney (29.4 μA ± 2.8) and bladder (13.8 μA ± 1). The Qi planes with greatest imbalance were the Shao Yang and Shao Yin. In conclusion, volunteers with TMD presented a pattern of Qi deficiency, and the most prevalent imbalance patterns identified were in the kidney and bladder coupled meridians and in the energetic planes Shao Yin (heart/kidney) and Shao Yang (triple energizer/gall bladder).