A library of TAL effector nucleases spanning the human genome

Kim, Yongsub,Kweon, Jiyeon,Kim, Annie,Chon, Jae Kyung,Yoo, Ji Yeon,Kim, Hye Joo,Kim, Sojung,Lee, Choongil,Jeong, Euihwan,Chung, Eugene,Kim, Doyoung,Lee, Mi Seon,Go, Eun Mi,Song, Hye Jung,Kim, Hwangbeo Nature Publishing Group, a division of Macmillan P 2013 Nature biotechnology Vol.31 No.3

Transcription activator–like (TAL) effector nucleases (TALENs) can be readily engineered to bind specific genomic loci, enabling the introduction of precise genetic modifications such as gene knockouts and additions. Here we present a genome-scale collection of TALENs for efficient and scalable gene targeting in human cells. We chose target sites that did not have highly similar sequences elsewhere in the genome to avoid off-target mutations and assembled TALEN plasmids for 18,740 protein-coding genes using a high-throughput Golden-Gate cloning system. A pilot test involving 124 genes showed that all TALENs were active and disrupted their target genes at high frequencies, although two of these TALENs became active only after their target sites were partially demethylated using an inhibitor of DNA methyltransferase. We used our TALEN library to generate single- and double-gene-knockout cells in which NF-κB signaling pathways were disrupted. Compared with cells treated with short interfering RNAs, these cells showed unambiguous suppression of signal transduction.

朝鮮《李仙圖》에 나타난 작가의 심미의식과 시대적 의의

金道榮(Kim Doyoung) 한국도교문화학회 2012 道敎文化硏究 Vol.36 No.-

본고에서는 한국회화사에서 김명국의 〈풍백도〉라는 오류를 필자가 최초로 발견하여 김명국의 〈철괴도〉로 바로잡았다. 정조시대 프로페셔날 화인 김홍도는 〈풍속화첩〉이라는 대표작으로 서민의 생활상을 자신의 화폭에 그려 넣은 조선의 대표적인 리얼리티 화가로 평가되지만, 필자의 분석은 그것은 제한된 소재이고 당시의 현실과 상당부분 타협한 내용들임을 밝히고 있다. 그런 그의 관념은 그의 작품 전편에 흐르고 있으며, 士大夫를 향한 의식지향이라는 김홍도의 인간적 고뇌로 읽어내었다. 아울러 통시적으로 기타 畵人 김명국, 심사정, 조석진 등의 〈철괴도〉와의 비교를 통해 그들의 〈철괴도〉에 투영된 작가의 예술정신과 시대적 의의를 밝히고 있다. 그리고 조선〈철괴도〉가 중국〈철괴도〉와 어떠한 차별점을 띠는지 집중 분석한 결과, 이 〈철괴도〉가 조선조의 향유계층인 상층양반사회에 수용되며 한국적으로 변형된 점, 日本에서는 원형그대로의 모습을 간직한 이유에 대해 韓?中?日문화와 정신의 차이에서 기인한 점을 밝히고 비교연구의 가능성을 제시하고 있다. This paper aims to assess the Korean modification of Tie kuai-Li in the Chosun Immortal paintings. Especially I focus that why the greatest painter Kim Hongdo(金弘道) can not paint the Immortal Iron Crutch -Li. Li Tie Kuai - the famous Immortal disabled begger with the Iron Crutch 鐵拐 and a bottle-gourd 葫蘆 offers a striking contrast to the other members of the group. Hideous, hairy, deformed, and scantily clad in filthy rags, he is the type of that repulsive legion preying upon a long-suffering public, which is moved to the giving of alms not so much by pity as by feelings of horror and fear. That is the reason why a painter Kim Hongdo wining the heart of the reformation ruler 正祖 of the Chosun Dynasty absolutely can not paint the Immortal disabled begger Iron Crutch -Li, a cripple. Kim Hongdo belonging to the middle class was a struggle for the upper classes and a person who compromised with reality. In this essay, I try to illuminate my initial findings and artistic spirit of 4 painters; 〈the Immortal Iron Crutch -Li(李仙圖)〉 painted by Kim meongguk(金明國) was first discovered by my investigation and reserch. Kim meongguk was also often more creative, unconventional, adventurous, and rebellious. With all his ability and descending from a noble family, Sim sazeng(沈師正) lived in obscurity all his life by the involvement system and had no way to work his will. Cho sukjin(趙錫晋) was a person with mild disposition and negative reconciliation during the Japanese Colonial Period. In conclusion, I am considering it in two bearings; The one is the transformation of the nobility in Immortal paintings, the two upper classes(兩班) of old Korea is living free from worldly cares. Tie kuai paintings losing the Yuan dynasty’s original form in the Chosun Immortal paintings are a mirror of the aristocrat of old Korea. The other is that these 4 Chosun painters was samely breaking up the Chinese Immortal Iron Crutch -Li by each artistic spirit. This is the distinguishing mark of 〈the Immortal Iron Crutch -Li〉 painted by Korean.

Homecare Kit를 활용한 수면무호흡 진단 서비스 개발

김동수(Dongsu-KIM),김도영(Doyoung-KIM),김동현(Donghyeon-KIM),김동겸(Donggyeom-KIM),김민규(Mingyu-KIM),김일곤(Ilgon-KIM) 한국정보과학회 2017 한국정보과학회 학술발표논문집 Vol.2017 No.12

The formation of a dielectric SiNxCy sealing layer using an atomic layer deposition technique

Kim, Doyoung,Kim, Soo-Hyun,Kim, Hyungjun Elsevier Science B.V., Amsterdam. 2015 Materials science in semiconductor processing Vol.29 No.-

Synergistic effects of dietary ascorbic acid, -tocopherol and arachidonic acid on non-specific immune response, tissue concentration and gonad histology in female broodstock Japanese eel, Anguilla japonica

Doyoung Kim,Seunghan Lee,Youngjin Park,Dae-Jung Kim,Dae-Jung Kim,Bae Ik Lee,Sungchul C.Bai 한국수산해양기술학회(구 한국어업기술학회) 2015 한국수산해양기술학회 학술발표대회 Vol.2015 No.10

UV-curing kinetics and performance development of <i>in situ</i> curable 3D printing materials

Kim, Ye Chan,Hong, Sungyong,Sun, Hanna,Kim, Myeong Gi,Choi, Kisuk,Cho, Jungkeun,Choi, Hyouk Ryeol,Koo, Ja Choon,Moon, Hyungpil,Byun, Doyoung,Kim, Kwang J.,Suhr, Jonghwan,Kim, Soo Hyun,Nam, Jae-Do Elsevier 2017 European polymer journal Vol.93 No.-

<P><B>Abstract</B></P> <P>As three-dimensional (3D) printing technology is emerging as an alternative way of manufacturing, the high resolution 3D printing device often requires systems such as drop jetting printing of <I>in situ</I> UV-curable photopolymers. Accordingly, the key issue is process control and its optimization to ensure dimensional accuracy, surface roughness, building orientation, and mechanical properties of printed structures, which are based on the time- and temperature-dependent glass transition temperature (<I>T<SUB>g</SUB> </I>) of the resin system under UV-curing. In this study, the UV-cure kinetics and <I>T<SUB>g</SUB> </I> development of a commercially available UV-curable acrylic resin system were investigated as a model system, using a differential scanning photocalorimeter (DPC). The developed kinetic model included the limited conversion of cure that could be achieved as a maximum at a specific isothermal curing temperature. Using the developed model, the <I>T<SUB>g</SUB> </I> was successfully described by a modified DiBenedetto equation as a function of UV curing. The developed kinetic model and <I>T<SUB>g</SUB> </I> development can be used to determine the 3D printing operating conditions for the overlay printing and <I>in situ</I> UV curing, which could ensure high-resolution and high-speed manufacturing with various UV-curing materials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> UV-cure kinetic analysis were applied to a commercial Multi-jet 3D printing material. </LI> <LI> The developed kinetic model included the limited conversion of cure by temperature. </LI> <LI> The <I>T<SUB>g</SUB> </I> was described by a modified DiBenedetto equation as a function of UV curing. </LI> <LI> The developed kinetic model showed an excellent agreement to isothermal experiments. </LI> <LI> The overlay printing time for each isothermal temperature was determined. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

N ‐(Biphenyl‐3‐ylmethyl)ethanamines as G protein‐biased agonists of 5‐HT 7 R

Kim Doyoung,Lee Jieon,Kwag Rina,Kim Hyunbin,Oh Hyunji,문봉진,Kim Hak Joong,Seong Jihye,Jeon Byungsun,Kang Taek,Choo Hyunah 대한화학회 2022 Bulletin of the Korean Chemical Society Vol.43 No.1

There has been much attention to biased ligands of G protein-coupled receptors (GPCRs) for potential pharmacological benefits. Recently, we reported N- ((6-chloro-2’-methoxy-[1,1’-biphenyl]-3-yl)methyl)ethanamine 1 as G proteinbiased agonist of 5-HT7R, which could be used as a chemical probe for the study on treatment discovery of autism spectrum disorder. Herein, we describe the synthesis of derivatives of the compound 1 and their biological evaluations in both G protein and β-arrestin signaling pathway. Total 16 compounds were synthesized and evaluated, and the compounds 3c, 3f, 3i, and 3p could be called as G protein-biased agonists like the compound 1. Among the four compounds, the compound 3c was the best in efficacy with an Emax value of 73% and the compound 3f was the most potent agonist with an EC50 value of 0.094 μM.

Luminescent Properties of Rare Earth Fully Activated Apatites, LiRE₉(SiO₄)₆O₂ (RE = Ce, Eu, and Tb): Site Selective Crystal Field Effect

Kim, Donghyeon,Park, Doyoung,Oh, Namgyeong,Kim, Jaegyeom,Jeong, Euh Duck,Kim, Seung-Joo,Kim, Sungyun,Park, Jung-Chul American Chemical Society 2015 Inorganic Chemistry Vol.54 No.4

<P>Novel LiCe9(SiO4)(6)O-2 and LiTb9(SiO4)(6)O-2 compounds have been successfully synthesized, and the site selectivity and occupancy of activator ions have been estimated including LiEu9(SiO4)(6)O-2 compound. The rare earth (RE) fully occupied compounds, as well as the RE partially occupied congeners are required for the assessment of site selectivity of RE (activator) ions in apatite-type compounds. The splitting energies of the 6H and 4F Wycoff positions of LiRE9(SiO4)(6)O-2 (RE = Ce, Eu, and Tb) compounds are calculated based on crystal field theory: Delta E-Ce(6H) = 3849.3 cm(-1), Delta E-Ce(4F) = 4228.1 cm(-1), Delta E-Eu(6H) = 3870.0 cm(-1), Delta E-Eu(4F) = 4092.8 cm(-1), Delta E-Tb(6H) = 3637.6 cm(-1), Delta E-Tb(4F) = 4396.1 cm(-1), indicating that the splitting energy for the 4F site is larger than that for the 6H site in all compounds; thus the absorption energy is higher for the 6H site. In apatite-type LiRE9(SiO4)(6)O-2 (RE = Ce, Eu, and Tb) compounds, the Ce3+ ions predominantly occupy the 4F site associated with the absorption band around 300 nm at lower Ce3+ concentration, and then enter the 6H site associated the absorption band around 245 nm. For the Eu3+-doped compounds, the 4F site and 6H site are mixed within the charge transfer band (CTB) between 220 and 350 nm. Eu3+ ions initially preferentially occupy the 6H site (around 290 nm) at lower Eu3+ concentration and subsequently enter the 4F site (around 320 nm) with increasing Eu3+ concentration. For the Tb3+-doped compounds, the absorption due to the two different sites is mixed within f-d absorption band between 200 and 300 nm. At lower Tb3+ concentration, the Tb3+ ions enter favorably 6H site around 240 nm and then enter 4F site around 270 nm. These compounds may provide a platform for modeling a new phosphor and application in the solid-state lighting field.</P>

장자사상(莊子思想)이 조선후기 사의화(寫意畵)에 끼친 심미경지 연구

김도영 ( Kim Doyoung ) 한국동양예술학회 2017 동양예술 Vol.34 No.-

In the late Joseon period (about 1700 ~ 1850), various changes were actively made in various fields such as politics and economy as well as society and culture. It was also the time of the most brilliant achievements in Korean painting history with the new paintings and the development of various styles. In particular, the fluctuations and changes of the class system have brought a tremendous change in the manner of the writers. It was not possible to actively cope with the changed order of the late Choson dynasty by the artistic work based on the three -At this point, Chuangtzu`s criticism, the logic of change and injustice were captured by progressive thinkers and used as a mechanism to criticize the system and to reflect the authenticity of morality. As a means of searching literally for refreshing the direction of life, The idea of “Chuangtzu” has become an alternative. The painting of the artist is a painting of the nobleman which emphasizes the feeling of the artist`s intention in the appearance of the object to be depicted, and draws attention to the spiritual world of the artist, the “Paint one`s mind”. The postwar history of the late Joseon Dynasty was greatly influenced by the artistic spirit of the Chuangtzu, who portrayed the liberation spirit of `Draw a meaning` that freed the mind and purifies the mind cleanly. Kang Se-hwang, Sim sa-jung, Lee In-sang, Kim Hong-do, Choi Buk, and Cho Hee-ryong are the characters who embraced the epistemology of the Chuangtzu and sought the possibility of new thought and embodied it in the work. Oriental painting has an artistic orientation to reveal his spiritual world, that is, to the “Draw a meaning” rather than the reproduction of natural objects. As a new method of socialization, they positively accepted the idea of “Chuangtzu”, escaped from the constraints of reality and took a free life or displayed a meeting on secular value, and escaped from the Neo-Confucianism explored aesthetic sense. In this way, it is analyzed that there are three major characteristics of the aesthetic and the aesthetic characteristics of the Chuangtzu`s thought on the painting of the late Joseon Dynasty. First, it is the Daegyo beaty of Ki-jin-ho-do. What the Chuangtzu denies is the “little craft” which means secular beauty and revealing to others. However, if the worldly beauty is denied, it will be possible to grasp the `A big beaty`, and if it transcends the pleasures of earthly delight, it will be able to realize the `Daelock` and pursue the `Daegyo`. Cho Hee-ryong`s < Hongmaedo Dalian > and Kim Hong-do`s < Nomaedo > combine the natural nature and the technique of handicraft to create a new painting of plum with the best artistic status. It is the Daegyo beaty of Ki-jin-ho-do. Secondly, this is a simple beauty of the Empty to reveal reality. This is a way of expressing the shape of the thread (the part drawn) as a `huh` (a part that is not drawn), which is a breakthrough expression method that has taken shape of the brush briefly. If the origin of the universe is regarded as being in the `“Empty to reveal reality” and the source of beauty as “Empty to reveal reality”, the method of expressing the figure in relation to not doing anything in a state that is not artificially modified is explained by the margin (huh). And, in fact, it is a line and a side. The paintings of Kim Hong-do`s “Sansangkwanmaedo” and Lee In-sang`s “Changbaigsando” In the marvelous work of margins and book lines, In stark contrast to the pilseon of the margins, the `jin` of the natural substance was expressed completely. Especially, Jeon ki`s the < Gyesanpomudo > is emphasized the fulness of the natural life conveyed by the Brief expressions with no color and By the dry effect of a dry brush. Third, it is a quaint beauty of so-yo-ja-jeog. True art is free from self-consciousness and free from social conventions. In addition, the external ugliness and bizarre expresses the sublime and beauty of the human intrinsic spirit even more truthfully and more potently. Here, Chuangtzu`s original intention was not to create a single artistic form, and through it, the person who realized the nature of the absurdity, even though the external form looks incongruous and ugly, It is emphasized to receive love. Through the ugliness, the judges who showed `Jin` the < Sunindohaedo > of Sim sa-jung, on the paintings of the < Goeseok do >, which is based on the paintings of the Gang se-hwang, painted with my fingertips on the < Pungseoryaguiin > of Choi buk. It can be seen that he deprived his unfortunate environment and painted a picture that seeks freedom and relaxed freedom. The late Joseon period was a period when the artist perceived that art could acquire eternal life-style when he constantly attempted self-transformation while denying and criticizing the previous fixed framework. At this time, the free spiritual aesthetics of the Chuangtzu who pursued the natural nature by eliminating compromise or norms with the world affects the painters of those days.

자율지능 로봇의 경로 계획 및 설명을 위한 거대 언어 모델 적용 방안 연구

김도영(Doyoung Kim),백한비(Hanbi Baek),김희연(Hee-Yeun Kim),김성우(Seong-Woo Kim) 대한전자공학회 2024 대한전자공학회 학술대회 Vol.2024 No.6

Focusing on the integration of Large Language Models (LLMs) in robotics, this research examines their role in path planning and providing linguistic explanations for autonomous intelligent robots. LLMs have demonstrated superior performance in natural language processing and problem-solving abilities, and various attempts are being made in the robotics field to utilize them in path planning. In this study, we propose a framework that enables LLMs to understand map data, plan paths, and provide linguistic explanations for decision-making processes. To achieve this, we enhance the performance of LLMs using incontext learning and fine-tuning methods and verify their performance through various evaluation metrics. Experimental results indicate that LLMs can perform more accurate path planning by providing time-aware path explanations. Future research will design a framework that integrates with ROS (Robot Operating System) and explore methods to condition LLMs to operate on map forms that reflect linguistic context and spatial experience.