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Yoo, Jisun,Jung, Young Mee,Hahn, Jong Hoon,Pyo, Dongjin Marcel Dekker 2010 Journal of immunoassay & immunochemistry Vol.31 No.4
<P>A quantitative analysis of prostate-specific antigen (PSA) in samples of human blood serum by fluorescence immunochromatography using monoclonal antibodies to PSA was developed. The fluorescence immunochromatographic analysis system is composed of anti-PSA-monoclonal antibody (mAb), fluorescence conjugates in detection solution, a immunochromatographic assay strip, and a laser fluorescence scanner. A fluorescence immunochromatographic analysis system was employed to detect PSA on the basis of the area ratio between the control line and the test line of the strip. Under optimal conditions, the area ratio was proportional to PSA concentration ranging from 0.72 to 46.0 ng/mL with a detection limit of 0.72 ng/mL.</P>
Cheolhee Yoo,임정호,박수민,Dongjin Cho 대한원격탐사학회 2020 大韓遠隔探査學會誌 Vol.36 No.4
Satellite-based land surface temperature (LST) has been used as one of the major parameters in various climate and environmental models. Especially, Moderate Resolution Imaging Spectroradiometer (MODIS) LST is the most widely used satellite-based LST product due to its spatiotemporal coverage (1 km spatial and sub-daily temporal resolutions) and longevity (> 20 years). However, there is an increasing demand for LST products with finer spatial resolution (e.g., 10-250 m) over regions such as urban areas. Therefore, various methods have been proposed to produce high-resolution MODIS-like LST less than 250 m (e.g., 100 m). The purpose of this review is to provide a comprehensive overview of recent research trends and challenges for the downscaling of MODIS LST. Based on the recent literature survey for the past decade, the downscaling techniques classified into three groups—kernel-driven, fusion-based, and the combination of kernel-driven and fusion-based methods—were reviewed with their pros and cons. Then, five open issues and challenges were discussed: uncertainty in LST retrievals, low thermal contrast, the nonlinearity of LST temporal change, cloud contamination, and model generalization. Future research directions of LST downscaling were finally provided.
Degradation of cyanobacterial toxin, microcystin LR, using chemical oxidants.
Pyo, Dongjin,Yoo, Jisun Marcel Dekker 2008 Journal of immunoassay & immunochemistry Vol.29 No.3
<P>Cyanobacterial toxins, microcystins, are very potent hepatotoxins and their occurrence has been reported all over the world. They could threaten human health when toxic Microcystis occurs in water supply reservoirs. In this study, the effects of several environmental factors on production and degradation of toxins produced by cyanobacteria in Lake Soyang have been studied. A new rapid quantification method of microcystins, using fluorescence for a detection signal and a lateral-flow-type immunochromatography as a separation system, was used. Chlorine, potassium permanganate, and hydrogen peroxide were used as chemical oxidants for the degradation of microcystin LR. When chlorine was used, the efficiency of degradation was the highest. The degradation reaction took 40 minutes.</P>
Pyo, Dongjin,Kim, Taemin,Yoo, Jisun Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.2
For the production of ethanol from freshwater cyanobacteria, a new pretreatment method using supercritical fluid was introduced. In this study, it was found that the supercritical fluid could penetrate inside the cell wall and help to liberate starch from cyanobacterial cells which resulted in the increase of the efficiency of ethanol production. For Microcystis aeruginosa, supercritical fluid pretreatment increased the amount of ethanol produced from cyanobacteria from 1.53 g/L to 2.66 g/L. For Anabaena variabilis, the amount of ethanol was increased from 1.25 g/L to 2.28 g/L. With use of supercritical fluid pretreatment, the efficiency of the process to obtain higher ethanol yields from freshwater cyanobacteria was improved upto 80%. The optimum temperature and pressure conditions for supercritical fluid pretreatment were determined as the temperature of $40^{\circ}C$ and the pressure of 120 atm. This study demonstrates the feasibility of using supercritical fluid pretreatment for ethanol production using freshwater cyanobacteria.
New trends in fluorescence immunochromatography.
Pyo, Dongjin,Yoo, Jisun Marcel Dekker 2012 Journal of immunoassay & immunochemistry Vol.33 No.2
<P>During the last decade, immunochromatographic techniques, in particular fluorescence labeled immunochromatography, have become more popular for the determination of low concentrations of analytes. It offers several potential advantages including highly sensitive detection and wide applications in clinical chemistry, bioanalysis, and environmental analysis. Currently, fluorescence labeled immunochromatography is not the exclusive preserve for a few specialists who are well experienced with antigen-antibody analysis, since the recent developments in fluorescence labeled immunochromatography have become widespread, from simple screening application to quantitative analysis of analytes. In this review, we assembled recent advances in the development and applications of fluorescence labeled immunochromatography techniques, considering their potential benefits in the future.</P>
Comparison of Matrices for Optimal Analysis of Synthetic Polymers Using MALDI-TOF Mass Spectrometry
( Hee-jin Yoo ),( Duck-hyun Kim ),( Yoon-ji Choi ),( Jung Hoon Choi ),( Moonhee Park ),( Dongjin Shin ),( Yoonseok Oh ),( Yangsun Kim ),( Kun Cho ) 한국질량분석학회 2020 Mass spectrometry letters Vol.11 No.4
Characterization of the various chemical aspects of composite polymers is important for quality control of manufactured polymers. In this study, we compared three suitable matrices (α cyano-4-hydroxycinnamic acid [CHCA], 2,5 dihydroxy benzoic acid [2,5-DHB], and dithranol), to characterize various synthetic polymers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Although the spectra obtained with the CHCA and 2,5-DHB matrices were generally good, in certain samples ghost peaks disappeared only when dithranol was used as the matrix. Furthermore, we examined the use of sodium trifluoroacetate (NaTFA) as an additive to reduce interference by metals and copolymers in the spectra. In conclusion, appropriate selection of a matrix, according to the characteristics of the polymer, and the use of additives to improve sensitivity are important considerations for polymer analysis and development.
Yoen Heera,Yoo Roh-Eul,Choi Seung Hong,Kim Eunkyung,오병모,Yang Dongjin,Hwang Inpyeong,Kang Koung Mi,Yun Tae Jin,Kim Ji-hoon,Sohn Chul-Ho 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.1
Objective: This study aimed to investigate the blood-brain barrier (BBB) disruption in mild traumatic brain injury (mTBI) patients with post-concussion syndrome (PCS) using dynamic contrast-enhanced (DCE) magnetic resonance (MR) imaging and automatic whole brain segmentation. Materials and Methods: Forty-two consecutive mTBI patients with PCS who had undergone post-traumatic MR imaging, including DCE MR imaging, between October 2016 and April 2018, and 29 controls with DCE MR imaging were included in this retrospective study. After performing three-dimensional T1-based brain segmentation with FreeSurfer software (Laboratory for Computational Neuroimaging), the mean Ktrans and vp from DCE MR imaging (derived using the Patlak model and extended Tofts and Kermode model) were analyzed in the bilateral cerebral/cerebellar cortex, bilateral cerebral/cerebellar white matter (WM), and brainstem. Ktrans values of the mTBI patients and controls were calculated using both models to identify the model that better reflected the increased permeability owing to mTBI (tendency toward higher Ktrans values in mTBI patients than in controls). The Mann-Whitney U test and Spearman rank correlation test were performed to compare the mean Ktrans and vp between the two groups and correlate Ktrans and vp with neuropsychological tests for mTBI patients. Results: Increased permeability owing to mTBI was observed in the Patlak model but not in the extended Tofts and Kermode model. In the Patlak model, the mean Ktrans in the bilateral cerebral cortex was significantly higher in mTBI patients than in controls (p = 0.042). The mean vp values in the bilateral cerebellar WM and brainstem were significantly lower in mTBI patients than in controls (p = 0.009 and p = 0.011, respectively). The mean Ktrans of the bilateral cerebral cortex was significantly higher in patients with atypical performance in the auditory continuous performance test (commission errors) than in average or good performers (p = 0.041). Conclusion: BBB disruption, as reflected by the increased Ktrans and decreased vp values from the Patlak model, was observed throughout the bilateral cerebral cortex, bilateral cerebellar WM, and brainstem in mTBI patients with PCS.