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김규완(Gyu Wan Kim),김윤상(Yun Sang Kim),Zhang Guang,Tokitada Hashimoto,Toshiaki Setoguchi,김희동(Heuy Dong Kim) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11
In this study basic research was carried out to obtain the fundamental data of the needle-free injection system with powder injection. Improved needle-free injector was designed and manufactured. In addition, we measured the velocity of the powder particles, this was performed by simply visualizing the macro mode traveling at supersonic velocity to measure the number of milliliters of the square particles with high spatial and temporal resolution using high-speed video camera. The velocity data were acquired by the particle tracking velocimetry method after image processing.
Piao, Hong-Guang,Lee, Hana,Yoon, Jungbum,Kim, Dong-Hyun,You, Chun-Yeol,Kim, Tae Wan IEEE 2010 IEEE transactions on magnetics Vol.46 No.6
<P> We have investigated a ratchet effect of magnetic domain wall in asymmetric sawtooth-shaped ferromagnetic nanowires under high frequency AC magnetic fields by means of micromagnetic simulation. The ratchet phenomenon has been intensively examined during the domain wall propagation with variation of the asymmetric sawtooth geometry of nanowires as well as variation of the AC external field frequency/strength. Very interestingly, it has been found that there is a strong correlation between the spatial frequency of asymmetrical notches and the effective driving frequency/strength of AC external fields. It is demonstrated that number of notches swept by domain wall ratchet motion per each half-cycle of the driving frequency is controlled under a GHz AC driving field. </P>
Xiao‐Juan Li,Guang-Ping DONG,Jian-Min FANG,Hong-Jian LIU,Wan-Lin GUO 한국곤충학회 2017 Entomological Research Vol.47 No.3
Monochamus alternatus is a destructive stem‐boring herbivore of Pinus massoniana, and the principal vector of pine wood nematode. To investigate the impacts of boring by M. alternatus larvae on the emission of volatile organic compounds (VOCs) from their host trees, the VOCs from uninfested and M. alternatus larvae infested P. massoniana trees were observed using a gas chromatograph–mass spectrometer. We detected 12, 9, 18 and 14 volatile organic compounds from infested xylem, infested phloem, uninfested xylem and uninfested phloem, respectively. In P. massoniana xylem, the boring of M. alternatus larvae induced cyclosativene, and inhibited 4‐carene, humulene, styrene, α‐phellandrene, β‐myrcene, β‐phellandrene and γ‐terpinene. The relative amounts of camphene, copacamphene, longicyclene, longifolene, tricyclene and α‐longipinene were significantly increased, and the relative amounts of α‐pinene and β‐pinene were significantly decreased by the boring behaviors of M. alternatus larvae. In P. massoniana phloem, the boring of M. alternatus larvae induced 2‐bornanone, copacamphene, longicyclene and α‐longipinene, and inhibited 2‐carene, 4‐carene, styrene, α‐phellandrene, β‐myrcene, β‐phellandrene, β‐pinene, γ‐terpinene and ο‐cymene. The relative amounts of camphene, caryophyllene and longifolene were significantly increased by the boring behaviors of M. alternatus larvae. The results indicate that the boring behaviors of M. alternatus larvae changed both the sorts and contents of the VOCs from P. massoniana trees.
Xiao‐Juan Li,Guang-Ping DONG,Jian-Min FANG,Hong-Jian LIU,Wan-Lin GUO 한국곤충학회 2018 Entomological Research Vol.48 No.4
The pine sawyer Monochamus alternatus Hope (Coleoptera: Cerambycidae) is a serious pest of several Pinus species, and the ectoparasitoid larvae of Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae) is an important natural enemy of this pest. The transcriptome of M. alternatus larvae was sequenced using the Illumina platform and immunity‐related genes were specifically analyzed. De novo assembly resulted in the identification of 24 241 unigenes, with a mean length of 1122 bp, in unparasitized M. alternatus larvae and 23 807 unigenes, with a mean length of 1140 bp, for parasitized larvae. Removal of redundant unigenes resulted in 26 095 all‐unigenes, of which 16 959 (64.99%) showed clear homology with some of the known genes in the National Center for Biotechnology Information nr database. Parasitization had notable effects on the transcriptome profile of M. alternatus larvae. In all, 2702 genes were differentially expressed in M. alternatus larvae after parasitization, with 1491 (55.18%) upregulated and 1211 (44.82%) downregulated. Moreover, expression levels of immunity‐related genes in M. alternatus larvae were markedly altered in response to parasitization by D. helophoroides. In conclusion, the transcriptome profiling data, especially the discovered of immunity‐related genes, help illustrate the molecular mechanisms of parasitism between D. helophoroides and M. alternatus and provide new insights into developing immunity regulation‐mediated control methods of M. alternatus.
( Ki Joo Kim ),( Jung Wan Oh ),( Guang Zhen Jin ),( Jung Ho Lee ),( Deuk Young Oh ),( Hyun Wook Kang ),( Hwan Mook Kim ),( Soo Hong Lee ),( Dong Woo Cho ),( Sang Tae Ahn ),( Jong Won Rhie ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.3
One of the main goals of bone tissue engineering is to develop suitable bio-acceptable materials for bone graft substitutes, especially for filling large defects. In the present study, porous poly(lactic-co-glycolic acid) (PLGA)-silica scaffolds were successfully fabricated using indirect microstereolithography technology. The scaffolds were evaluated in vitro by analyzing their microscopic structure, porosity, and stiffness. Following in vitro osteogenic induction, human adipose-derived stromal cells (ADSCs) were seeded in the scaffolds, and the expression levels of osteocalcin mRNA and collagen type I protein were detected by RT-PCR and Western blotting, respectively. We further evaluated the osteoinductive efficacy of the scaffolds in vivo by implanting human ADSC-seeded scaffolds into athymic mice. The silica particles improved the mechanical strength of PLGA scaffolds, and the PLGA-silica scaffolds exhibited better osteogenic potential in vitro than conventional PLGA scaffolds. The in vivo osteogenic events after scaffold implantation were consistent with those observed in vitro. This study demonstrates the potential of PLGA-silica scaffolds seeded with human ADSCs as a useful tool for bone tissue engineering.