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Do Huynh Luong,Neelesh Sharma,Nameun Kim,Jiao Jiao Zhang,Nisansala Chandimali,Mrinmoy Ghosh,Meeta Gera,Taeho Kwon,Dong-Kee Jeong 한국발생생물학회 2017 한국발생생물학회 학술발표대회 Vol.2017 No.8
Bovine mammalian gland has biopotential in therapeutic protein production and could be used as a model in further lactation researches. In this study, we have isolated bovine mammary gland-derived epithelial cells (BMECs) from Korean Holstein dairy cattle and themselves show differential dynamic ability in in-vitro culture. BMECs enables to form lobulo-alveolar structure, express milk production related gene. Functional studies indicated that BMECs secret exogeneous antibacterial fragment-Bovine Lactoferricin B (bLfcin-B), which is inserted in PiggyBac system, and this bioactive fragment inhibits the growths of Escherichia coli and Staphylococcus aureus. These data demonstrated that BMECs open new scope in either bioactive fragment, heading to prevent the spread of mastitis or post-mastitis damage in dairy graze and could be an ideal bioreactor for antibacterial proteins.
Huynh, Do Luong,Zhang, Jiao Jiao,Chandimali, Nisansala,Ghosh, Mrinmoy,Gera, Meeta,Kim, Nameun,Park, Yang Ho,Kwon, Taeho,Jeong, Dong Kee Elsevier 2018 Biochemical and biophysical research communication Vol.503 No.4
<P><B>Abstract</B></P> <P>Pancreatic ductal adenocarcinoma (PDAC) is a major malignant phenotype in pancreatic cancer, which is one of the most death causes by cancer in the world. PDAC developed from pancreatic intra-epithelial neoplasms (PanINs) and poorly diagnosed at early stages. Beside of high drug resistance, metastasis is the great concern during pancreatic cancer treatment. SALL4 expression is inherent in the upregulations of endothelial mesenchymal transition (EMT) genes and therefore promoting cancer metastasis. Furthermore, some of evidences indicated reactive oxygen species (ROS) is also influent to metastasis and self-antioxidant capacity seems a gold standard for successful metastasis rate. In this study, we have found the role Spalt like protein 4 (SALL4) to PDAC proliferation, mobility and its regulation to mitochondrial ROS via FoxM1/Prx III axis. It is possible that SALL4 mainly induces endothelial-mesenchymal transition (EMT) phenotype and favors ROS loss to facilitate metastasis efficiency in PDAC cells. Therefore, SALL4 might be a promising marker for PDAC treatment and targeting SALL4 would benefit anti-proliferative and anti-metastasis therapies.</P> <P><B>Highlights</B></P> <P> <UL> <LI> SALL4 promotes stemness traits and metastatic phenotype <I>in vitro</I>. </LI> <LI> SALL4 expression positively impacts PDAC-derived tumor growth. </LI> <LI> SALL4 regulates intracellular ROS via FoxM1/Prx III by activation of ERK1/2. </LI> </UL> </P>
Han Choe,Minh Quan Nguyen,Do Hyung Kim,Hye Ji Shim,Huynh Kim Khanh Ta,Thi Luong Vu,Thi Kieu Oanh Nguyen,Jung Chae Lim 한국분자세포생물학회 2023 Molecules and cells Vol.46 No.12
Recombinant immunotoxins (RITs) are fusion proteins consisting of a targeting domain linked to a toxin, offering a highly specific therapeutic strategy for cancer treatment. In this study, we engineered and characterized RITs aimed at mesothelin, a cell surface glycoprotein overexpressed in various malignancies. Through an extensive screening of a large nanobody library, four mesothelin-specific nanobodies were selected and genetically fused to a truncated Pseudomonas exotoxin (PE24B). Various optimizations, including the incorporation of furin cleavage sites, maltose-binding protein tags, and tobacco etch virus protease cleavage sites, were implemented to improve protein expression, solubility, and purification. The RITs were successfully overexpressed in Escherichia coli, achieving high solubility and purity post-purification. In vitro cytotoxicity assays on gastric carcinoma cell lines NCI-N87 and AGS revealed that Meso(Nb2)-PE24B demonstrated the highest cytotoxic efficacy, warranting further characterization. This RIT also displayed selective binding to human and monkey mesothelins but not to mouse mesothelin. The competitive binding assays between different RIT constructs revealed significant alterations in IC50 values, emphasizing the importance of nanobody specificity. Finally, a modification in the endoplasmic reticulum retention signal at the C-terminus further augmented its cytotoxic activity. Our findings offer valuable insights into the design and optimization of RITs, showcasing the potential of Meso(Nb2)-PE24B as a promising therapeutic candidate for targeted cancer treatment.
Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
Zhang, Jiao Jiao,Wang, Xian Zhong,Kwon, Taeho,Huynh, Do Luong,Chandimali, Nisansala,Kim, Nameun,Kang, Tae Yoon,Ghosh, Mrinmoy,Gera, Meeta,Lee, Sang Baek,Lee, Sung Jin,Lee, Wang Shik,Kim, Seong Bong,Mo MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.8
<P>As an innovative technology in biological applications—non-thermal plasma technique—has recently been applied to living cells and tissues. However, it is unclear whether non-thermal plasma treatment can directly regulate the growth and development of livestock. In this study, we exposed four-day-incubated fertilized eggs to plasma at 11.7 kV for 2 min, which was found to be the optimal condition in respect of highest growth rate in chickens. Interestingly, plasma-treated male chickens conspicuously grew faster than females. Plasma treatment regulated the reactive oxygen species homeostasis by controlling the mitochondrial respiratory complex activity and up-regulating the antioxidant defense system. At the same time, growth metabolism was improved due to the increase of growth hormone and insulin-like growth factor 1 and their receptors expression, and the rise of thyroid hormones and adenosine triphosphate levels through the regulation of demethylation levels of growth and hormone biosynthesis-related genes in the skeletal muscles and thyroid glands. To our knowledge, this study was the first to evaluate the effects of a non-thermal plasma treatment on the growth rate of chickens. This safe strategy might be beneficial to the livestock industry.</P>
Zhang, Jiao Jiao,Kang, Tae Yoon,Kwon, Taeho,Koh, Hyebin,Chandimali, Nisansala,Huynh, Do Luong,Wang, Xian Zhong,Kim, Nameun,Jeong, Dong Kee American Society for Microbiology 2019 Infection and immunity Vol.87 No.3
<P><I>Gallibacterium anatis</I> is a pathogen associated with peritonitis and salpingitis in chickens and other avian species. Novel safety prevention strategies are urgently needed because of widespread multidrug resistance and antigenic diversity.</P><P><I>Gallibacterium anatis</I> is a pathogen associated with peritonitis and salpingitis in chickens and other avian species. Novel safety prevention strategies are urgently needed because of widespread multidrug resistance and antigenic diversity. The objective of this study was to produce a specific chicken egg yolk antibody and evaluate its protective response against a <I>G. anatis</I> infection model in 4-week-old chicks. Enzyme-linked immunosorbent assays showed that hens immunized with the recombinant N terminus of <I>Gallibacterium</I> toxin A (GtxA-N) had significantly increased antibody titers against GtxA-N in serum and egg yolk IgY. Western blotting showed that IgY antibody had specificity against GtxA-N in the egg yolks of immunized hens. The growth of <I>G. anatis</I> in brain heart infusion (BHI) broth and agar was significantly inhibited by the GtxA-N-specific IgY antibody. The protective effects of the specific IgY antibody were evaluated in <I>G. anatis</I>-infected chicks after intramuscular injection (10 mg/ml). The anti-GtxA-N antibody titers in the sera of <I>G. anatis</I>-challenged chicks following an injection of specific IgY antibody were significantly higher than those of the control and the nonspecific IgY groups, but lower lesion scores for the peritoneum, liver, and duodenum were found after specific IgY antibody treatment. The results from this study suggest that the GtxA-N-specific IgY antibody could potentially improve the protective response against <I>G. anatis</I> infection in chicks.</P>