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- 저자 : Diao, Yun Fei (검색결과 212 건)
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匡調元 圓光大學校 韓醫學硏究所 1994 원광한의학 Vol.4 No.1
The constitution of human is a stable state with the specificity of function and metabolism, which can distinguish one from another during the process that he is born, grow up and senile. This state may usually decide the specialty of physical response, sensitivity to the cause and type of disease. In Western medicine, there is a middle zone between that of disease and that of normal state, whether it can be disease or not. Based on these concept, the constitutional pathology is suggested. The constitution of human is classified into five types in the part of pathology. In this study, a experiment was designed for the scientific access of Chinese constituion theory and constitutional food therapy.
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Diao, Qi,Li, Xiaoyang,Diao, Mengxiao,Lee, Yong-Ill,Liu, Hong-Guo Elsevier 2018 JOURNAL OF COLLOID AND INTERFACE SCIENCE - Vol.522 No.-
<P><B>Abstract</B></P> <P><B>Hypothesis</B></P> <P>Our previous studies have shown that the metal nanoparticle/polymer composite structures fabricated at the liquid/liquid interface have good reusability but lower catalytic activity for heterogeneous reactions in aqueous solutions. This should be attributed to the poor water wettability and more compact structure of the polymer matrices. Therefore, it should be possible to improve the catalytic activity through designing and fabricating a porous composite structure with good water wettability.</P> <P><B>Experiments</B></P> <P>A modified liquid/liquid interface adsorption and fabrication method was used. An aqueous solution of copper acetate and a chloroform/DMF mixed solution of PS-<I>b</I>-PAA acted as the two phases. Through spontaneous emulsification, self-assembly of the polymer molecules with Cu<SUP>2+</SUP> ions in the droplets, and adsorption of the formed spherical micelles and nanofibers to the planar liquid/liquid interface, a porous composite microstructure was formed.</P> <P><B>Findings</B></P> <P>This structure consisted of nanofiber-connected nanospheres which have a PS core and a PAA corona. Tiny and well-dispersed Cu nanoparticles were embedded in the hydrophilic corona and were adsorbed on the nanofibers surface as well. After physical cross-linking with 1,6-diaminohexane, the composite material exhibited high catalytic activity and good reusability for the reactions in aqueous solutions. For example, the rate constant for the reduction of <I>p</I>-nitroaniline reached 1965 s<SUP>−1</SUP> g<SUP>−1</SUP>.</P> <P><B>Graphical abstract</B></P> <P>Block copolymer/copper composite microstructure with highly heterogeneous catalytic activity was fabricated successfully at the liquid/liquid interface.</P> <P>[DISPLAY OMISSION]</P>
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A single vesicle-vesicle fusion assay for in vitro studies of SNAREs and accessory proteins
Diao, Jiajie,Ishitsuka, Yuji,Lee, Hanki,Joo, Chirlmin,Su, Zengliu,Syed, Salman,Shin, Yeon-Kyun,Yoon, Tae-Young,Ha, Taekjip Nature Publishing Group, a division of Macmillan P 2012 NATURE PROTOCOLS -ELECTRONIC EDITION- Vol.7 No.5
SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins are a highly regulated class of membrane proteins that drive the efficient merger of two distinct lipid bilayers into one interconnected structure. This protocol describes our fluorescence resonance energy transfer (FRET)-based single vesicle-vesicle fusion assays for SNAREs and accessory proteins. Both lipid-mixing (with FRET pairs acting as lipophilic dyes in the membranes) and content-mixing assays (with FRET pairs present on a DNA hairpin that becomes linear via hybridization to a complementary DNA) are described. These assays can be used to detect substages such as docking, hemifusion, and pore expansion and full fusion. The details of flow cell preparation, protein-reconstituted vesicle preparation, data acquisition and analysis are described. These assays can be used to study the roles of various SNARE proteins, accessory proteins and effects of different lipid compositions on specific fusion steps. The total time required to finish one round of this protocol is 3??6 d.
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Diao, Yun-Fei,Kim, Hong-Rye,Han, Rong-Xun,Kim, Myung-Yoon,Park, Chang-Sik,Jin, Dong-Il The Korean Society of Animal Reproduction 2010 Reproductive & developmental biology Vol.34 No.3
Current developments in IVF and animal cloning have resulted in increasing demand for large quantities of oocytes and ovarian follicles at specific stages of development. These medical and scientific needs may be met by developing an optimal culture system for preantral follicles. In this study, we investigated the growth of porcine preantral follicle cultures in different media and in the presence and absence of serum. Follicles were manually dissected from ovaries obtained from prepubertal gilts at a local slaughterhouse, and cultured for 3 days in M199 or NCSU23 medium supplemented with porcine FSH, transferrin, L-ascorbic acid and insulin. Follicle diameters were measured on day 1 and 3 of culture. In Experiment 1, the effect of supplementing culture medium with fetal calf serum (FCS) on porcine preantral follicle growth was examined. In the group of cultures supplemented with FCS, follicle diameter after 3 days of culture, survival rate and antrum formation rate in the FCS group were significantly higher than those of the control group. In Experiment 2, the effects of culture medium (M199 and NCSU23) on follicle growth were compared. Follicle diameters were increased in the M199 group, compared with those in NCSU23 (p<0.05), but we observed no significant differences in survival and antrum formation rates between cultures grown in the two media. In conclusion, supplementation of the culture medium with serum enhances preantral follicle growth and antrum formation, and M199 is superior to NUSU23 for porcine preantral follicle culture in vitro.
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Diao, Li-Jun,Dong, Kan,Yin, Shao-Bo,Tang, Jing,Chen, Jie The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.5
The traction motors in electric multiple unit (EMU) trains are powered by AC-DC-AC converters, and the DC link voltage is generated by single phase PWM converters, with a fluctuation component under twice the frequency of the input catenary AC grid, which causes fluctuations in the motor torque and current. Traditionally, heavy and low-efficiency hardware LC resonant filters parallel in the DC side are adopted to reduce the ripple effect. In this paper, an analytical model of the ripple phenomenon is derived and analyzed in the frequency domain, and a ripple control scheme compensating the slip frequency of rotor vector control systems without a hardware filter is applied to reduce the torque and current ripple amplitude. Then a relatively simple discretization method is chosen to discretize the algorithm with a high discrete accuracy. Simulation and experimental results validate the proposed ripple control strategy.
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Diao Zongguang,김현정,이상엽 한국비교정부학회 2008 한국비교정부학보 Vol.12 No.1
By comparing with the international hotel, the internal hotel have huge difference in scope、strength、the degree of famous、the ability of capital operating. The collectivization is the major representation. Our hotels should properly breakthrough in the hotel industry, and capture the market economic hotel, the creation of national brands and enhance core competitiveness, the high tech transformation of the traditional hotel business model, increase the development of human resources upgrading the level of groups, and promote the development of the Group.
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Diao, Yun-Fei,Kenji, Naruse,Han, Rong-Xun,Lin, Tao,Oqani, Reza-K.,Kang, Jung-Won,Jin, Dong-Il The Korean Society of Animal Reproduction 2013 Reproductive & developmental biology Vol.37 No.2
Developmental potential of cloned embryos is related closely to epigenetic modification of somatic cell genome. The present study was to investigate the effects of applying histone deacetylation inhibitor, trichostatin A (TSA) to activated porcine embryos on subsequent development of porcine parthenogenetic and nuclear transfer embryos. Electrically activated oocytes were treated with 5 nM TSA for different exposure times (0, 1, 2 and 4 hr) and then the activated embryos were cultured for 7 days. The reconstructed embryos were treated with different concentrations of 0, 5, 10 and 25 nM TSA for 1 hr. Also 5 nM TSA was tested with different exposure times of 0, 0.5, 1, 2 and 4 hr. And fetal fibroblast cells were treated with 50 nM TSA for 1, 2 or 4 hr and with 5 nM TSA for 1 hr. Cumulus-free oocytes were enucleated and reconstructed by TSA-treated donor cells and electrically fused and cultured for 6 days. In parthenogenetic activation experiments, 5 nM TSA treatment for 1 hr significantly improved the percentage of blastocyst developmental rates than the other groups. Total cell number of blastocysts in 1 hr group was significantly higher than other groups or control. Similarly, blastocyst developmental rates of porcine NT embryos following 5 nM TSA treatment for 1 hr were highest. And the reconstructed embryos from donor cells treated by 50 nM TSA for 1 hr improved the percentage of blastocyst developmental rates than the control group. In conclusion, TSA treatment could improve the subsequent blastocyst development of porcine parthenogenetic and nuclear transfer embryos.
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Screening for Metastatic Osteosarcoma Biomarkers with a DNA Microarray
Diao, Chun-Yu,Guo, Hong-Bing,Ouyang, Yu-Rong,Zhang, Han-Cong,Liu, Li-Hong,Bu, Jie,Wang, Zhi-Hua,Xiao, Tao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4
Objective: The aim of this study was to screen for possible biomarkers of metastatic osteosarcoma (OS) using a DNA microarray. Methods: We downloaded the gene expression profile GSE49003 from Gene Expression Omnibus database, which included 6 gene chips from metastatic and 6 from non-metastatic OS patients. The R package was used to screen and identify differentially expressed genes (DEGs) between metastatic and non-metastatic OS patients. Then we compared the expression of DEGs in the two groups and sub-grouped into up-regulated and down-regulated, followed by functional enrichment analysis using the DAVID system. Subsequently, we constructed an miRNA-DEG regulatory network with the help of WebGestalt software. Results: A total of 323 DEGs, including 134 up-regulated and 189 down-regulated, were screened out. The up-regulated DEGs were enriched in 14 subcategories and most significantly in cytoskeleton organization, while the down-regulated DEGs were prevalent in 13 subcategories, especially wound healing. In addition, we identified two important miRNAs (miR-202 and miR-9) pivotal for OS metastasis, and their relevant genes, CALD1 and STX1A. Conclusions: MiR-202 and miR-9 are potential key factors affecting the metastasis of OS and CALD1 and STX1A may be possible targets beneficial for the treatment of metastatic OS. However, further experimental studies are needed to confirm our results.
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DIAO, HUAIAN,CAO, YONGLIN 한국전산응용수학회 2006 Journal of applied mathematics & informatics Vol.20 No.1
The classical perturbation theory is extended to the weighted Kronecker product linear systems $W(A{\bigotimes}B)W\chi=h$. Upper bounds are derived for the normwise condition number.
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