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Weishi Yu,Yuequn Wang,Yongqing Li,Yun Deng,Zequn Wang,Wuzhou Yuan,Dali Li,Chuanbing Zhu,Xueying Zhao,Xiaoyang Mo,Wen Huang,Na Luo,Yan Yan,Karen Ocorr,Rolf Bodmer,Xiushan Wu 한국분자세포생물학회 2008 Molecules and cells Vol.26 No.5
The Bric-a-brac, Tramtrack, Broad-complex (BTB) domain is a protein-protein interaction domain that is found in many zinc finger transcription factors. BTB containing proteins play important roles in a variety of cellular functions including regulation of transcription, regulation of the cytoskeleton, protein ubiquitination, angiogenesis, and apoptosis. Here, we report the cloning and characterization of a novel human gene, KLHL31, from a human embryonic heart cDNA library. The cDNA of KLHL31 is 5743 bp long, encoding a protein product of 634 amino acids containing a BTB domain. The protein is highly conserved across different species. Western blot analysis indicates that the KLHL31 protein is abundantly expressed in both embryonic skeletal and heart tissue. In COS-7 cells, KLHL31 proteins are localized to both the nucleus and the cytoplasm. In primary cultures of nascent mouse cardiomyocytes, the majority of endogenous KLHL31 proteins are localized to the cytoplasm. KLHL31 acts as a transcription repressor when fused to GAL4 DNA-binding domain and deletion analysis indicates that the BTB domain is the main region responsible for this repression. Overexpression of KLHL31 in COS-7 cells inhibits the transcriptional activities of both the TPA-response element (TRE) and serum response element (SRE). KLHL31 also significantly reduces JNK activation leading to decreased phosphorylation and protein levels of the JNK target c-Jun in both COS-7 and Hela cells. These results suggest that KLHL31 protein may act as a new transcriptional repressor in MAPK/JNK signaling pathway to regulate cellular functions.
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Preparation of b-Ca3(PO4)2 bioceramic powderfrom calcium carbonate and phosphoric acid q
Yun Zhang,Guangfu Yin,Shifu Zhu,Dali Zhou,Yuehua Wang,Yong Li,Lin Luo 한국물리학회 2005 Current Applied Physics Vol.5 No.5
When preparing b-tricalcium phosphate (b-TCP) with Ca(NO3)2 or Ca(OH)2 as the raw materials, because of the problem ofltering, it is dicult to industrialize traditional methods. In this paper,b-TCP was prepared with calcium carbonate and phospho-ric acid. According to XRD spectrum, the precursor of this system is octacalcium phosphate (OCP) [Ca8H2(PO4)6Æ5H2O] instead ofnon-stoichiometric hydroxyapatite (HA) obtained from traditional methods, and thermodynamics mechanism of the reaction wasalso studied. The crystalb-TCP powders were obtained after the precursor had been calcined at 8501150.C for 2 h. SEM imagesshowed that hardening could eliminate the reunion to a great extent, and obtain dispersiveb-TCP powders with a particle diameterbetween 0.5 and 2.0l m. The main advantages of this process over traditional methods are the fast reaction rate, the easy ltering,the ne particle size and the minimum impurity..
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Study on the Isomerization of Maleic Acid to Fumaric Acid without Catalyst
Zhuo Gao,Wangmi Chen,Xiaoting Chen,Dali Wang,Shouzhi Yi 대한화학회 2018 Bulletin of the Korean Chemical Society Vol.39 No.8
Fumaric acid is an important food additive and industrial intermediate compound. The traditional methods of producing fumaric acid were catalyzed by maleic acid isomerization. In this study, isomerization of maleic acid in water without catalyst was investigated at elevated temperature, which addressed the problem of sewage discharge. HPLC analysis showed maleic acid converted into fumaric acid and small amount of malic acid simultaneously. The effects of concentration of maleic acid, reaction temperature, reaction time, and stirring on the yield of fumaric acid were investigated. The optimum reaction conditions were also explored. The results showed the isomerization of maleic acid reached equilibrium at about 1?h, stirring did not affect the reaction rate, and conversion due to the monomolecular mechanism. In order to achieve ?zero emission,? the recycle of filter liquor was also studied. The pH of filter liquor changing signifies the lower pH was in favor of the conversion of maleic acid to fumaric acid. The high yield and recyclability suggested that this process had promising application in fumaric acid production.
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Seed dormancy overcoming and seed coat structure change in Leucaena leucocephala and Acacia nilotica
Mulik Abbaker Ibrahim Yousif,Yan Rong Wang,Chen Dali 한국산림과학회 2020 Forest Science And Technology Vol.16 No.1
Leucaena leucocephala and Acacia nilotica are two arboreal legumes with several uses in agriculture, nutritious forage and livelihoods, fence posts, wood production, biofuel production, charcoal, firewood, shading, hedges, windbreaks, and improvement of soil fertility. The seed dormancy is common in Leucaena and Acacia species, and it creates difficulties in seed testing and planting. The aim of this study is to determine the effective methods of sulfuric acid and hot water due to overcoming dormancy in seeds of those species. Most pretreatments significantly (p<0.05) reduced hard seed content and enhanced germination percentage when compared to nontreated seeds. Effectiveness of water and acid pretreatments increased when increasing in duration time from 3 to 60 min. The best recommended time pretreatment of sulfuric acid 60 min for A. nilotica, and 3 min of hot water for Leucaena. These treatments all reduced hard seeds to 0% and did not cause damage to the seeds during the germination. Further, the lens and hilum both were identified as primary sites for water uptake into the seeds of Leucaena. Whereas, the lens was recognized as the original site of water intake in A. nilotica. In addition, the seed coat anatomy identified that the palisade epidermal layer was dense, and thickness tissue had been prevented imbibition of water in Leucaena and A. nilotica.
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Da Li,Jianjun Ma,Chaosheng Wang,Xiaojuan Gao,Mingjing Fang 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.8
This paper presents a new method to evaluate the piping risk of unconfined sandy cobble aquifers during the dewatering of deep foundation pits. Firstly, the possibility of piping is judged according to the particle size distribution and pore diameter of the sandy cobble stratum. Secondly, the critical hydraulic gradients of particles with different sizes are calculated according to the pipe flow model. Thirdly, the hydraulic gradient around the foundation pit during the dewatering process is calculated by numerical method. Then, combined with the distribution of hydraulic gradient around the foundation pit and the critical hydraulic gradients of particles with different sizes, the range of moveable particles with different sizes in the surrounding stratum is evaluated. Finally, according to the sandy cobble aquifers conditions and the influence of particle movement on the stratum, the level of piping risk is determined. This method was successfully applied to evaluate the piping risk of Tawan Station in Luoyang Metro Line No.1, and according to the risk evaluation the pensile curtain was recommended for the retaining structure in this case. From the satisfied applications, this method is a reference for the evaluation of piping risk and selection of retaining structure when considering the impact of the dewatering in deep foundation pit with unconfined aquifers on the surrounding stratum.
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