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Daojie Wang,Cuiling Yang,Long Dong,Jiacheng Zhu,Jianping Wang,Shufeng Zhang 한국식물학회 2015 Journal of Plant Biology Vol.58 No.4
Brassica napus is a dicotyledonous plant in the family Brassicaceae. It is an important oil crop which has been widely cultivated over the world. However, drought stress is a very important threatening to B. napus production. In this study, two B. napus strains with different resistance to drought stress were treated with 200 g/L PEG-6000 as drought simulation agent (marked as S, R)and two controls were treated with 1/2 Hoagland medium(marked as ST, RT). With the help of Illumina paired-end RNA-seq technology and de novo assembly by Trinity, we obtained 107,294 Unigenes (integrated four sample results) with an average length of 834 bp and N50 1245. Of these Unigenes, 84,768, 92,545, 54,857, 28,129, 75,278 were assigned to NR, NT, Swiss-Prot, COG and GO database respectively. A total of 46,861 were mapped to 128 pathways by BLAST comparison against the KEGG database. In order to have an overall understanding of the unique genes’ expression difference among four samples, we utilized RPKM to calculate Unigene expression, and to identify differentially expressed genes. Besides, 22,414 SSRs were developed in this study.
Cuiling Zhao,Yuchao Bai,Hao Wang 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.4
Laser powder bed fusion (LPBF) provides an effective and economical solution for fabricating multi-material components of complex structures as it entails a layer-wise manufacturing process. The feasibility and reliability of depositing AlSi10Mg alloy on the wrought AA6061 alloy substrate using the LPBF process were studied. The study includes the analysis of metallurgical quality, microstructure evolution, mechanical properties, and corrosion behaviour of the multi-material parts before and after heat treatment. The interface region, decorated with epitaxial growth, shows excellent metallurgical bonding without apparent defects of pores and cracks. LPBF AlSi10Mg comprises fine equiaxed grains and coarse columnar grains on the boundary and inside the molten pool, respectively. They were replaced by large Si particles after heat treatment without altering the grain morphology and <100>//BD (building direction) texture. The as-built multi-material part exhibits a low ultimate tensile strength of 192.8 ± 3.4 MPa, similar to that of wrought AA6061, and a higher elongation (13.6 ± 0.5%) than the LPBF AlSi10Mg alloy (9.4 ± 0.2%). In addition, the ultimate tensile strength and elongation of the multi-material part were slightly improved after heat treatment. Compression testing showed that, in contrast to single-alloy parts, the multi-material part achieved moderate strength and good compressive capacity under both as-built and heat-treated conditions. Interestingly, the galvanic corrosion effects in the interface region are suppressed for both as-built and heat-treated multi-material parts. Moreover, the as-built multi-material sample has a higher corrosion resistance than the heat-treated one. This study verifies the feasibility of efficiently manufacturing a reliable, excellent, and low-cost multi-material component combining conventional and additive manufacturing processes.
Yuetao Li,Yongkun Zhao,Cuiling Wang,Xuexing Zheng,Hualei Wang,Weiwei Gai,Hongli Jin,Feihu Yan,Boning Qiu,Yuwei Gao,Nan Li,Songtao Yang,Xianzhu Xia 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.2
Rift Valley fever (RVF) is an acute, febrile zoonotic disease that is caused by the RVF virus (RVFV). RVF is mainly prevalent on the ArabianPeninsula, the African continent, and several islands in the Indian Ocean near southeast Africa. RVFV has been classified by the WorldOrganisation for Animal Health (OIE) as a category A pathogen. To avoid biological safety concerns associated with use of the pathogen inRVFV neutralization assays, the present study investigated and established an RVFV pseudovirus-based neutralization assay. This study usedthe human immunodeficiency virus (HIV) lentiviral packaging system and RVFV structural proteins to successfully construct RVFVpseudoviruses. Electron microscopy observation and western blotting indicated that the size, structure, and shape of the packagedpseudoviruses were notably similar to those of HIV lentiviral vectors. Infection inhibition assay results showed that an antibody against RVFVinhibited the infective ability of the RVFV pseudoviruses, and an antibody neutralization assay for RVFV detection was then established. Thisstudy has successfully established a neutralization assay based on RVFV pseudoviruses and demonstrated that this method can be used toeffectively evaluate antibody neutralization.
Yuanpeng Fu,Yaqun He,Hangchao Chen,Cuiling Ye,Qichang Lu,Rongnian Li,Weining Xie,Jie Wang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.79 No.-
The present work focuses on simultaneous recycling of Li and Co from crushed products of mixedelectrode materials using mixed organic acids, in which benzenesulfonic acid and formic acid werecooperatively used as the leaching reagents. Results show that the optimal leaching efficiency of 97% Coand 99% Li were obtained under the conditions of 1.3 mol/L benzenesulfonic acid, 1.5 mol/L formic acid, asolid to liquid (S/L) ratio of 30 g/L, and 40 min reaction time at 50 C. Meanwhile, the leaching of Li and Cofits well to logarithmic rate model with apparent activation energy of 32.7 and 47.0 kJ/mol in this givenleaching system, respectively. Besides, cobalt was directly recovered from the leach liquor as pure cobaltbenzene sulfonic with the recovery efficiency of 99%, and lithium can be entirely precipitated by addingphosphoric acid. Further, the reaction mechanism involves the leaching-hydrating-complexing model ofLiCoO2 particles was proposed based on the dissolution behavior of metals and then verified bymorphological and phase characterization (i.e. FT-IR, XRD and SEM-EDS) of the recycling product. Thewhole process is found to be effective and sustainable for recovery of Li, Co and graphite from mixedindustrial crushing product of spent LIBs.
A High-Performance Self-Powered UV Photodetector Based on SnO2 Mesoporous Spheres @ TiO2
Yuewu Huang,Qingjiang Yu,Jinzhong Wang,Xiaochao Li,Yuan Yan,Shiyong Gao,Feifei Shi,Dongbo Wang,Cuiling Yu 대한금속·재료학회 2015 ELECTRONIC MATERIALS LETTERS Vol.11 No.6
A novel self-powered UV photodetector (UVPD) based on the photoelectrochemical cell (PECC) has been constructed using the TiO2 coated SnO2 mesoporous spheres (SnO2-MS@TiO2). This self-powered UVPD displays a higher photocurrent density compared to the UVPD with the pure SnO2-MS. By means of external quantum efficiency (EQE), UV-vis absorption, and electrochemical impedance measurements, we scrutinize the intrinsic role of the TiO2 coating layer on the photocurrent enhancement. Under UV irradiation, this UVPD exhibits a high on/off ratio of 11519, a fast rise time of 0.007 s and decay time of 0.006 s, together with the excellent visible-blind characteristic and linear optical signal response. The self-powered photodetector is a promising candidate for application in high-sensitivity and high-speed UVPDs.