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Chung, Namhyun 한국응용생명화학회 2001 Journal of Applied Biological Chemistry (J. Appl. Vol.44 No.4
Contaminated sites are often abandoned for an extended period of time, thus organopollutants becoming sequestered. The information on the initial concentrations of the contaminants would be helpful for the economic bioremediation of the chemicals. The present study estimated the initial concentrations of atrazine and phenanthrene through multiple regression analyses using soil properties and the amount of chemicals available in situ. Percentage mineralized or extracted was best correlated with organic C or logarithm of organic C, and the $R^2$ values were 0.548 and 0.894 for atrazine and phenanthrene, respectively. Estimation of the initial concentration of the chemicals was then calculated from both the percentage mineralized or extracted and the amount of chemicals extracted or mineralized. Results showed that the estimation of the initial concentration of the chemical at the time of contamination is feasible.
Chung, Eunsu,Gao, Rongke,Ko, Juhui,Choi, Namhyun,Lim, Dong Woo,Lee, Eun Kyu,Chang, Soo-Ik,Choo, Jaebum Royal Society of Chemistry 2013 Lab on a chip Vol.13 No.2
<P>We report the rapid and highly sensitive trace analysis of mercury(ii) ions in water using a surface-enhanced Raman scattering (SERS)-based microdroplet sensor. Aptamer-modified Au/Ag core-shell nanoparticles have been fabricated and utilized as highly functional sensing probes. All detection processes for the reaction between mercury(II) ions and aptamer-modified nanoparticles were performed in a specially designed microdroplet channel. Small water droplets that included sample reagents were separated from each other by an oil phase that continuously flowed along the channel. This two-phase liquid-liquid segmented flow system prevented the adsorption of aggregated colloids to the channel walls due to localized reagents within encapsulated droplets. The result was reduced residence time distributions. The limit of detection (LOD) of mercury(II) ions in water was determined by the SERS-based microdroplet sensor to be below 10 pM, which is three orders below the EPA-defined maximum contaminant level. This combination of a SERS-based microfluidic sensor with aptamer-based functional nanoprobes can be used for in-the-field sensing platforms, due to its size and simplicity.</P>
( Ji Young Lee ),( Namhyun Chung ),( Yong Kwon Lee ) 한국응용생명화학회(구 한국농화학회) 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.1
Aged black garlic (ABG) was extracted with 20% ethanol and water (crude extracts) and fractionated into three categories (>10, 3-10, and <3 kDa). The effect of crude extract supplements on anti-My-10 hybridoma cell growth and IgG1 antibody production was investigated in suspension culture with a chemically defined protein-free medium. We observed that supplementation of ABG to the cell culture medium stimulated anti-My-10 hybridoma cell growth and production of IgG1 antibody, particularly with fractionated ABG of low molecular weight. The stimulation depended upon the concentration and the size of the fractionated ABG. We also found that the growth promoting activity was not correlated with high antibody production. These results suggest that fractionated ABG is a novel and promising alternative as an animal cell culture supplement.
JEON, JU-HYUN,PARK, JUN-HWAN,CHUNG, NAMHYUN,LEE, HOI-SEON ational Association for Food Protection 2014 Journal of food protection Vol.77 No.8
<P>The acaricidal activities of an active material derived from Rosmarinus officinalis oil and its relative monoterpene ketones were determined using fumigant and contact toxicity bioassays against Tyrophagus putrescentiae and were compared with that of a commercial acaricide (benzyl benzoate). The active component of R. officinalis oil, isolated by silica gel column chromatography and high-performance liquid chromatography, was identified as camphor, based on various spectroscopic analyses. In the fumigant toxicity bioassay, camphor (2.25 μg/cm3) was 5.58 times more active than benzyl benzoate (12.56 μg/cm3) against T. putrescentiae, followed by (+)-camphor (3.89 μg/cm3) and (−)-camphor (5.61 μg/cm3). In the contact toxicity bioassay, camphor (1.34 μg/cm2) was 6.74 times more toxic than benzyl benzoate (9.03 μg/cm2) against T. putrescentiae, followed by (+)-camphor (2.23 μg/cm2) and (−)-camphor (2.94 μg/cm2). These results indicate that camphor and its derivatives are very useful as potential control agents against stored food mites regardless of the application method.</P>
Anti-Diabetic Effects of Red Rose Flowers in Streptozotocin-Induced Diabetic Mice
Ju, Jae Eun,Joo, Yong Hoon,Chung, Namhyun,Chung, Soo Yeon,Han, Sung Hee,Lee, Yong Kwon The Korean Society for Applied Biological Chemistr 2014 Applied Biological Chemistry (Appl Biol Chem) Vol.57 No.4
Diabetes mellitus is a common chronic metabolic disease that is of increasing concern, because it frequently leads to complications such as cardiovascular diseases and cancer. Plants have been studied as a potential source for anti-diabetics to supplement dietary modifications, insulin, and other medications. The components of green tea polyphenols can be changed by processes such as browning. This processing method was applied to the red rose flower to determine whether the processed or non-processed rose flower has an anti-diabetic effect on the streptozotocin-induced diabetic mouse. Aqueous extracts of processed and non-processed rose flowers were analyzed using liquid chromatography/mass spectrometry. The results show that the polyphenol content decreased with browning. The hemoglobin A1c level, an indicator of long-term diabetes, in diabetic mice after administration of extracts of browned rose flowers for 24 and 48 h were lower than those after administration of extracts of non-browned rose flowers. Moreover, the activity of aspartate transaminase, which is often high in diabetic patients, was low in all groups treated with rose flowers, whether they were non-browned or browned. Taken together, these results indicate that extracts from red rose flowers have long-term anti-diabetic effects, and that this effect is independent of the level of polyphenols in the extract.