MLL4 Regulates the Progression of Non-Small-Cell Lung Cancer by Regulating the PI3K/AKT/SOX2 Axis

Yang Yang,Rongfang Qiu,Qiaoyou Weng,Ziwei Xu,Jingjing Song,Siyu Zhao,Miaomiao Meng,Dengke Zhang,Chunli Kong,Hailin Wang,Min Xu,Zhongwei Zhao,Jiansong Ji 대한암학회 2023 Cancer Research and Treatment Vol.55 No.3

Purpose Mixed-lineage leukemia protein 4 (MLL4/KMT2D) is a histone methyltransferase, and its mutation has been reported to be associated with a poor prognosis in many cancers, including lung cancer. We investigated the function of MLL4 in lung carcinogenesis. Materials and Methods RNA sequencing (RNA-seq) in A549 cells transfected with control siRNA or MLL4 siRNA was performed. Also, we used EdU incorporation assay, colony formation assays, growth curve analysis, transwell invasion assays, immunohistochemical staining, and in vivo bioluminescence assay to investigate the function of MLL4 in lung carcinogenesis. Results We found that MLL4 expression was downregulated in non–small cell lung cancer (NSCLC) tissues compared to adjacent normal tissues and tended to decrease with disease stage progression. We analyzed the transcriptomes in control and MLL4- deficient cells using high-throughput RNA deep sequencing (RNA-seq) and identified a cohort of target genes, such as SOX2, ATF1, FOXP4, PIK3IP1, SIRT4, TENT5B, and LFNG, some of which are related to proliferation and metastasis. Our results showed that low expression of MLL4 promotes NSCLC cell proliferation and metastasis and is required for the maintenance of NSCLC stem cell properties. Conclusion Our findings identify an important role of MLL4 in lung carcinogenesis through transcriptional regulation of PIK3IP1, affecting the PI3K/AKT/SOX2 axis, and suggest that MLL4 could be a potential prognostic indicator and target for NSCLC therapy.

Ablation resistance under different heat fluxes of HfC‑ZrC‑SiC multiphase coating prepared by supersonic atmospheric plasma spraying for C/C composites

Yang Yang,Chun Zhao,Qiaojuan Gong,Jie Wang 한국세라믹학회 2020 한국세라믹학회지 Vol.57 No.2

To prevent the ablation of C/C composites, an HfC-ZrC-SiC multiphase coating was prepared by supersonic atmospheric plasma spraying. The morphology and microstructure of the coating were examined by X-ray diffraction and scanning electron microscopy, respectively. The as-prepared coating showed a compact structure with a few defects. The ablation resistance test of the coatings was carried out using an oxyacetylene torch under different heat fluxes. The samples ablated under different heat fluxes showed different ablation properties and ablation morphologies. During the ablation, the thickness and weight of the coating decreased with an increase in the heat flux from 2.38 to 4.18 MW/m2. Under the heat flux of 2.38 MW/m2, the coating remained intact because of its low surface temperature and the pinning effect of the newly formed HfSiO4 and ZrSiO4 phases. With an increase in the heat flux, the surface temperature increased beyond the melting point of HfO2 and ZrO2 and the coating suffered a violent flush of the oxyacetylene torch, which resulted in severe evaporation of the oxides and the weakening of the pinning effect.

The Prevalence and Genotype of Human Papillomavirus from Patients with Genital Warts in Eastern Guangdong Province

Luo, Zhao-Yun,Chen, Qiang,Yang, Hui,Lin, Min,Chen, Chan-Yu,Yang, Chun,Yang, Li-Ye Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.14

Background: Low-risk human papillomavirus (LR-HPV) infection is the main cause of genital warts. LRHPV genotypes 6 and 11 are associated with genital warts, but there have only been a few published studies about the genotype-specific prevalence of HPV in genital warts in China. The objective of our study was to assess the prevalence of HPV genotypes for clinical cases involving both men and women and to evaluate the potential benefit of a quadrivalent (genotypes 6, 11, 16, and 18) HPV vaccine in eastern Guangdong province of China. Materials and Methods: A total of 696 eligible patients with genital warts were enrolled during the period Aug 2009 through Oct 2014. Specimens were collected from genital warts, the HPV GenoArray test was used for HPV detection and genotyping, which could detect 21 HPV genotypes, including genotypes 6, 11, 16, and 18. Results: Among the 696 cases, 675 samples were successfully genotyped. The median age of patients was 32.1 years (range, 16-67 years). The most prevalent genotypes were HPV-6 (285/675, 42.2%), HPV-11 (265/675, 39.3%), HPV-52 (52/675, 7.7%), HPV-16 (51/675, 7.56%), HPV-81 (50/675, 7.40%) and HPV-58 (37/675, 5.48%). Low-risk genotypes predominated, with a prevalence of 96.59%. The cumulative prevalence of genotypes 6 and 11 was 78.7% (531/675), the cumulative prevalence of genotypes 16 and 18 was 11.6% (78/675), and the cumulative prevalence of genotypes 6, 11, 16, and 18 was 82.5% (557/675). Conclusions: Our results provide strong evidence that, in eastern Guangdong, different from Western countries, the most prevalent low risk HPV genotypes in patients with genital warts are 6, 11 and 81. The quadrivalent HPV vaccine could prevent 82.5% of genital warts in eastern Guangdong.

Effect of Prior Microstructures on Cementite Dissolution Behavior During Subcritical Annealing of High Carbon Steels

Xiao‑Yu Zhao,Xian‑Ming Zhao,Chun‑Yu Dong,Yang Yang,Huai‑Bin Han 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.6

The variation of the morphology and distribution of cementite particles in different prior structures with spheroidizingannealing process has been proceeded in this paper. It is found that the dissolution and coarsening progresses of variousinitial structures in spheroidizing annealing process are quite asynchronous due to the different interlamellar spacing. Thedissolution rate of degenerated pearlite (D-P) with finer interlamellar spacing is faster. The granular cementite in the spheroidizedmicrostructure is fine, uniform and dense. The mean diameter of spherical cementite is refined to 233 nm. However,the initial structure of degenerated pearlite (D-P) is highly sensitive to the austenitization temperature. The cementite iseasy to be coarsened under high austenitizing temperature. The coarsening is accompanied by the gradual increase of theCr content in the cementite, which increases the stability of the cementite. Therefore, the optimal austenitizing temperaturefor degenerated pearlite (D-P) is suggested to be 770 °C.

Preliminary Report on the Breeding of Robust and Resistant-NPV and High Quality Silkworm Race 'Shengming No.1' for Summer-autumn Rearing

Zhao, Yuan,Chen, Kepin,Yao, Qing,Wu, Yang-Chun,Zhang, Jian,Guo, Xijie Korean Society of Sericultural Science 2006 International Journal of Industrial Entomology Vol.13 No.2

Seveval Chinese and Japanese varieties with good characters were used in the breeding. After 5 years (15 generations), a pair of robust and high quality silkworm variety with NPV resistance was bred by means of a combination of crossing and pedigree selection complemented by the selection of NPV resistance. The variety was identified jointly nationwide in 2003 and 2004, and appraised by National Mulberry and Silkworm Appraising Committee. Results are as follows: its cocooning rate is over 93%, shell rate 23-25%, filament length 1200-1300 meters, reelability 75-88%, Length of non-broken cocoon filament 900-1100 meters, raw silk rate 17-19%, neatness 95-97 points, and cocoon crop, cocoon shell weight and raw silk weight per 10000 larvae is higher than those of the control variety by 7-10%, 14-19% and 14-18%, respectively. The variety is not only robust, resistant to high temperature and NPV, easy to rear, uniform in hatching, molting and maturing, but also lays more eggs, and its fecundity is high. It is suitable to rear in the Yangtze River Basin, the Yellow River basin and the Pearl River basin of China.

Anti-Neuroinflammatory Effects of Fucoxanthin via Inhibition of Akt/NF-κB and MAPKs/AP-1 Pathways and Activation of PKA/CREB Pathway in Lipopolysaccharide-Activated BV-2 Microglial Cells

Zhao, Dong,Kwon, Seung-Hwan,Chun, Yoon Sun,Gu, Ming-Yao,Yang, Hyun Ok Springer-Verlag 2017 Neurochem Res Vol.42 No.2

MTHFR C677T Polymorphism and Colorectal Cancer Risk in Asians, a Meta-analysis of 21 Studies

Yang, Zhen,Zhang, Xie-Fu,Liu, Hong-Xiang,Hao, Yong-Shun,Zhao, Chun-Lin Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Background: Previous studies concerning the association between methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism and colorectal cancer risk in Asian populations generated conflicting results. A meta-analysis was therefore performed to allow a more reliable estimate of any link. Methods: Relevant studies concerning the association between the MTHFR C677T polymorphism and risk of colorectal cancer were included into this meta-analysis. The quality of the studies was assessed according to a predefined scale. Odds ratios (ORs) and 95% confidence intervals (CIs) were determined for this gene-disease association using fixed or random effect models according to the heterogeneity between included studies. Results: Finally, 21 studies with a total of 6692 cases and 8266 controls were included. Meta-analyses showed that there was an obvious association of the MTHFR 677T allele with decreased risk of colorectal cancer (OR = 0.91, 95%CI=0.85-0.98, P=0.011). Subgroup analyses by country further identified this association, with dietary folate as the main source of heterogeneity. Conclusion: The MTHFR 677T allele is associated with a lower risk of colorectal cancer in Asian populations, and there is effect modification by population plasma folate.

A Novel Multi-Path Routing Algorithm Based on Clustering for Wireless Mesh Networks

( Chun-xiao Liu ),( Yan Zhang ),( E Xu ),( Yu-qiang Yang ),( Xu-hui Zhao ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.4

As one of the new self-organizing and self-configuration broadband networks, wireless mesh networks are being increasingly attractive. In order to solve the load balancing problem in wireless mesh networks, this paper proposes a novel multi-path routing algorithm based on clustering (Cluster_MMesh) for wireless mesh networks. In the clustering stage, on the basis of the maximum connectivity clustering algorithm and k-hop clustering algorithm, according to the idea of maximum connectivity, a new concept of node connectivity degree is proposed in this paper, which can make the selection of cluster head more simple and reasonable. While clustering, the node which has less expected load in the candidate border gateway node set will be selected as the border gateway node. In the multi-path routing establishment stage, we use the intra-clustering multi-path routing algorithm and inter-clustering multi-path routing algorithm to establish multi-path routing from the source node to the destination node. At last, in the traffic allocation stage, we will use the virtual disjoint multi-path model (Vdmp) to allocate the network traffic. Simulation results show that the Cluster_MMesh routing algorithm can help increase the packet delivery rate, reduce the average end to end delay, and improve the network performance.

Patent Ductus Arteriosus and Pulmonary Valve Stenosis in A Patient with 18p Deletion Syndrome

Chun-Hong Xie,Jian-Bin Yang,Fang-Qi Gong,Zheng-Yan Zhao 연세대학교의과대학 2008 Yonsei medical journal Vol.49 No.3

We report on a patient with a partial deletion on the short arm of chromosome 18 (del 18p), who presented with dysmorphic features and delayed developmental milestones as well as with a patent ductus arteriosus (PDA) and pulmonary valve stenosis (PS). Several forms of congenital heart disease (CHD) are found in about 10% of patients with del (18p), but coexisting PDA and PS have not been reported. Del (18p) must be considered in patients with characteristic phenotypic abnormalities and congenital heart disease, including a combination of PDA and PS.

Stem cells from human exfoliated deciduous teeth attenuate trigeminal neuralgia in rats by inhibiting endoplasmic reticulum stress

Yang, Zhijie,Wang, Chun,Zhang, Xia,Li, Jing,Zhang, Ziqi,Tan, Zhao,Wang, Junyi,Zhang, Junyang,Bai, Xiaofeng The Korean Pain Society 2022 The Korean Journal of Pain Vol.35 No.4

Background: The treatment of trigeminal neuralgia remains a challenging issue. Stem cells from human exfoliated deciduous teeth (SHED) provide optimized therapy for chronic pain. This study aimed to investigate the mechanisms underlying the attenuation of trigeminal neuralgia by SHED. Methods: Trigeminal neuralgia was induced by chronic constriction injury of the infraorbital nerve. The mechanical threshold was assessed after model establishment and local SHED transplantation. Endoplasmic reticulum (ER) morphology and Caspase12 expression in trigeminal ganglion (TG) was evaluated as well. BiP expression was observed in PC12 cells induced by tunicamycin. Results: The local transplantation of SHED could relieve trigeminal neuralgia in rats. Further, transmission electron microscopy revealed swelling of the ER in rats with trigeminal neuralgia. Moreover, SHED inhibited the tunicamycin-induced up-regulated expression of BiP mRNA and protein in vitro. Additionally, SHED decreased the up-regulated expression of Caspase12 mRNA and protein in the TG of rats caused by trigeminal neuralgia after chronic constriction injury of the infraorbital nerve mode. Conclusions: This findings demonstrated that SHED could alleviate pain by relieving ER stress which provide potential basic evidence for clinical pain treatment.