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      • KCI등재

        Isolation and Cytotoxic Potency of Endophytic Fungi Associated with Dysosma difformis, a Study for the Novel Resources of Podophyllotoxin

        Hoa Thi Tran,Giang Thu Nguyen,Hong Ha Thi Nguyen,Huyen Thi Tran,Quang Hong Tran,Quang Ho Tran,Ngoc Thi Ninh,Phat Tien Do,Ha Hoang Chu,Ngoc Bich Pham 한국균학회 2022 Mycobiology Vol.50 No.5

        Endophytic fungi are promising sources for the production of podophyllotoxin-an important anticancer compound, replacing depleted medical plants. In this study, the endophytes asso- ciated with Dysosma difformis-an ethnomedicinal plant species were isolated to explore novel sources of podophyllotoxin. Fifty-three endophytic fungi were isolated and identified by morphological observation and ITS-based rDNA sequencing, assigning them to 27 genera in 3 divisions. Fusarium was found the most prevalent genus with a colonization frequency of 11.11%, followed by Trametes (9.26%) and Penicillium (7.41%). Phylogenetic trees were constructed for the endophytic fungi community in two collection sites, Ha Giang and Lai Chau, revealing the adaptation of the species to the specific tissues and habitats. Cytotoxic activity of endophytic fungal extracts was investigated on cancer cell lines such as SK-LU-1, HL-60, and HepG2, demonstrating strong anti-cancer activity of six isolates belonging to Penicillium, Trametes, Purpureocillium, Aspergillus, and Ganoderma with IC50 value of lower than 10 mg/mL. The presence of podophyllotoxin was indicated in Penicillium, Trametes, Aspergillus and for the first time in Purpureocillium and Ganoderma via high-performance liquid chromatography, which implied them as a potential source of this anti- cancer compound.

      • KCI등재

        Enhancing Removal of Acid Orange II by Heterogeneous Catalytic Ozonation Using ZnO Nanoparticles-Modified Fly Ash Composite

        Hoang Van Hung,Chu Thi Hong Huyen,Nguyen Thi Dong,Nguyen Thi Hong Vien,Nguyen Thu Huyen,Luu Thi Cuc,Van Huu Tap,Nguyen Hoang,Duong Dinh Tuan,Nguyen Van Huu Luong 한국화학공학회 2024 Korean Journal of Chemical Engineering Vol.41 No.5

        In this study, heterogeneous catalytic ozonation using fl y ash (FA) modifi ed ZnO nanoparticles to the decolorization of acid orange II (AOII) was studied. The combination of ZnO and fl y ash via an incipient wetness impregnation method to form ZOFA composites created a synergistic eff ect that led to effi cient catalytic ozonation activity towards AOII degradation and decolorization compared with individual ozonation processes. From the experimental results, ZOFA composite with the optimal weight ratio between ZnO NPs and FA was 20 wt% (denoted as ZOFA-20) that exhibited outstanding catalytic ozonation activity. Moreover, through other investigations including initial pH conditions and catalyst dosage, AOII could be eff ectively and optimally eliminated by 0.5 g of ZOFA-20 with an initial pH value of 11. The corresponding k value was measured as 0.248 min −1 with a maximum TOC removal content of 77.27%. Besides, ZOFA-20 could show high catalytic ozonation activity even at highly concentrated AOII concentrations while it could also retain its excellent stability and reusability over 5 continuous AOII removal cycles. From the experimental and electron paramagnetic resonance (EPR) results, hydroxyl radical ( · OH) was the main active species during the catalytic ozonation of AOII. The present study provides a promising approach to prepare novel composite-based fl y ash that would be applicable for removing azo dye and other dye pollutants in water via a catalytic ozonation strategy.

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        Determinants of Human Resource Accounting Disclosures: Empirical Evidence from Vietnamese Listed Companies

        Duc Hieu PHAM,Thi Huyen CHU,Thi Minh Giang NGUYEN,Thi Hong Lam NGUYEN,Thi Nhinh NGUYEN 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.7

        This paper aims to analyze whether company characteristics are potential determinants of human resource accounting (HRA) disclosure practices by Vietnamese listed companies. It examines the human resource disclosure level of 204 companies by content analysis of these companies’ annual reports. The study has relied on a multiple linear regression to test the association between a number of corporate attributes and the extent of human resource disclosure in companies’ annual reports. The extent of human resource disclosure was measured using unweighted human resource disclosure index. The explanatory variables considered in this study were firm size, firm age, profitability, leverage, industry profile, and auditor type. The results revealed that the most influential variable for explaining firms’ variation in human resource disclosure is firm size followed by firm age and profitability. Thus, it can be concluded that firm size, firm age and profitability are major predictors that may affect the variety of HRA disclosure practices on firms listed in the Vietnam Stock Exchange. However, neither industry profile nor auditor type seems to explain differences in human resource disclosure practices between Vietnamese listed firms, indicating that company’s industry profile and auditor type are not a matter for the company to disclose HRA information.

      • KCI등재

        Genome‑wide identification, organization, and expression profiles of the chicken fibroblast growth factor genes in public databases and Vietnamese indigenous Ri chickens against highly pathogenic avian influenza H5N1 virus infection

        Truong Anh Duc,Tran Ha Thi Thanh,Chu Nhu Thi,Nguyen Huyen Thi,부 티 하오,Hong Yeojin,송기덕,Dang Hoang Vu,홍영호 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.4

        Objective: Fibroblast growth factors (FGFs) play critical roles in embryo development, and immune responses to infectious diseases. In this study, to investigate the roles of FGFs, we performed genome-wide identification, expression, and functional analyses of FGF family members in chickens. Methods: Chicken FGFs genes were identified and analyzed by using bioinformatics approach. Expression profiles and Hierarchical cluster analysis of the FGFs genes in different chicken tissues were obtained from the genome-wide RNA-seq. Results: A total of 20 FGF genes were identified in the chicken genome, which were classified into seven distinct groups (A-F) in the phylogenetic tree. Gene structure analysis revealed that members of the same clade had the same or similar exon-intron structure. Chromosome mapping suggested that FGF genes were widely dispersed across the chicken genome and were located on chromosomes 1, 4-6, 9-10, 13, 15, 28, and Z. In addition, the interactions among FGF proteins and between FGFs and mitogen‑activated protein kinase (MAPK) proteins are limited, indicating that the remaining functions of FGF proteins should be further investigated in chickens. Kyoto encyclopedia of genes and genomes pathway analysis showed that FGF gene interacts with MAPK genes and are involved in stimulating signaling pathway and regulating immune responses. Furthermore, this study identified 15 differentially expressed genes (DEG) in 21 different growth stages during early chicken embryo development. RNA-sequencing data identified the DEG of FGFs on 1- and 3-days post infection in two indigenous Ri chicken lines infected with the highly pathogenic avian influenza virus H5N1 (HPAIV). Finally, all the genes examined through quantitative real-time polymerase chain reaction and RNA-Seq analyses showed similar responses to HPAIV infection in indigenous Ri chicken lines (R2 = 0.92– 0.95, p<0.01). Conclusion: This study provides significant insights into the potential functions of FGFs in chickens, including the regulation of MAPK signaling pathways and the immune response of chickens to HPAIV infections.

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