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최시원(Siwon Choi),이동경(Dokyoung Lee),김동규(Dong Kyue Kim),나중채(Joong Chae Na),심정섭(Jeong Seop Sim) 한국정보과학회 2009 정보과학회 컴퓨팅의 실제 논문지 Vol.15 No.1
문자열 불포함 문제에 대한 연구는 최근 들어 여러 분야에서 활발히 진행되어 왔다. 문자열 집합 F가 주어질 때, F내의 어떤 문자열도 포함하지 않는 문자열을 F에 대한 공통비상위문자열이라 하고 공통비상위문자열 중에서 가장 긴 유한길이의 문자열을 최장공통비상위문자열이라한다. 본 논문에서는 공통비상위문자열과 관련된 연구 결과들을 제시한다. 먼저 기존의 공통비상위문자열에 대한 접미사 그래프 모델과 달리 접두사를 이용하여 직관적인 그래프 모델링이 가능함을 증명한다. 다음으로, 상수 크기의 알파벳에 대해 정의된 문자열 집합 F의 모든 문자열들의 길이의 합을 N라 할 때 O(N)시간에 접두사 그래프를 생성하고 이를 이용하여 최장공통비상위문자열을 찾는 알고리즘을 제시한다. Recently, the string non-inclusion related problems have been studied vigorously. Given a set of strings F over a constant size alphabet, consider a string χ such that χ does not include any string in F as a substring. We call χ a Common Non-SuperString(CNSS for short) of F. Among the CNSS's of F, the longest one with finite length is called the Longest Common Non-SuperString(LCNSS for short) of F. In this paper, we first propose a new graph model using prefixes of F. Next, we suggest an O(N)-time algorithm for finding the LCNSS of F, where N is the sum of the lengths of all the strings in F.
A Plant Virus-Based Vector System for Gene Function Studies in Pepper
Choi, Boram,Kwon, Sun-Jung,Kim, Myung-Hwi,Choe, Siwon,Kwak, Hae-Ryun,Kim, Mi-Kyeong,Jung, Choonkyun,Seo, Jang-Kyun American Society of Plant Biologists 2019 Plant Physiology Vol.181 No.3
<P>A plant virus-based system enables transient gain- and loss-of-function studies in pepper, a highly recalcitrant species for genetic transformation.</P><P>While pepper (<I>Capsicum annuum</I>) is a highly recalcitrant species for genetic transformation studies, plant virus-based vectors can provide alternative and powerful tools for transient regulation and functional analysis of genes of interest in pepper. In this study, we established an effective virus-based vector system applicable for transient gain- and loss-of-function studies in pepper using <I>Broad bean wilt virus2</I> (BBWV2). We engineered BBWV2 as a dual gene expression vector for simultaneous expression of two recombinant proteins in pepper cells. In addition, we established enhanced and stable expression of recombinant proteins from the BBWV2-based dual vector via coexpression of a heterologous viral suppressor of RNA silencing. We also developed a BBWV2-based virus-induced gene silencing (VIGS) vector, and we successfully silenced the phytoene desaturase gene (<I>PDS</I>) using the BBWV2-based VIGS vector in various pepper cultivars. Additionally, we optimized the BBWV2-based VIGS system in pepper by testing the efficiency of <I>PDS</I> gene silencing under different conditions. This BBWV2-based vector system represents a convenient approach for rapid and simple analysis of gene functions in pepper.</P>
Characterization of the Effects of Silver Nanoparticles on Liver Cell Using HR-MAS NMR Spectroscopy
Siwon Kim,현진원,Jinhee Choi,Bobae Kwon,Sangmi Lee,Sosun Kim,김석만 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.6
AgNPs (silver nanoparticles) has been widely used for the commercial products, which have antimicrobial agent, medical devices, food industry and cosmetics. Despite, AgNPs have been reported as toxic to the mammalian cell, lung, liver, brain and other organs and many researchers have investigated the toxicity of AgNPs. In this study, we investigated toxicity of the AgNPs to the liver cell using metabolomics based on HRMAS NMR (High Resolution Magic Angle Spinning Nuclear Magnetic Resonance) technics, which could apply to the intact tissues or cells, to avoid the sample destruction. Target profiling and multivariative statistical analysis were performed to analyze the 1D ^1H spectrum. The results show that the concentrations of many metabolites were affected by the AgNPs in the liver cell. The concentrations of glutathione (GSH), lactate,taurine, and glycine were decreased and most of amino acids, choline analogues, and pyruvate were increased by the AgNPs. Moreover, the levels of the metabolites were recovered upto similar level of metabolites in the normal cell by the pre-treatment of NAC, external antioxidant. The results suggest that the depletion of the GSH by the AgNPs might induce the conversion of lactate and taurine to the pyruvate.