<P>A plant virus-based system enables transient gain- and loss-of-function studies in pepper, a highly recalcitrant species for genetic transformation.</P><P>While pepper (<I>Capsicum annuum</I>) is a highly recalcitrant ...
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https://www.riss.kr/link?id=A107446181
2019
-
SCI,SCIE,SCOPUS
학술저널
867-880(14쪽)
0
상세조회0
다운로드다국어 초록 (Multilingual Abstract)
<P>A plant virus-based system enables transient gain- and loss-of-function studies in pepper, a highly recalcitrant species for genetic transformation.</P><P>While pepper (<I>Capsicum annuum</I>) is a highly recalcitrant ...
<P>A plant virus-based system enables transient gain- and loss-of-function studies in pepper, a highly recalcitrant species for genetic transformation.</P><P>While pepper (<I>Capsicum annuum</I>) is a highly recalcitrant species for genetic transformation studies, plant virus-based vectors can provide alternative and powerful tools for transient regulation and functional analysis of genes of interest in pepper. In this study, we established an effective virus-based vector system applicable for transient gain- and loss-of-function studies in pepper using <I>Broad bean wilt virus2</I> (BBWV2). We engineered BBWV2 as a dual gene expression vector for simultaneous expression of two recombinant proteins in pepper cells. In addition, we established enhanced and stable expression of recombinant proteins from the BBWV2-based dual vector via coexpression of a heterologous viral suppressor of RNA silencing. We also developed a BBWV2-based virus-induced gene silencing (VIGS) vector, and we successfully silenced the phytoene desaturase gene (<I>PDS</I>) using the BBWV2-based VIGS vector in various pepper cultivars. Additionally, we optimized the BBWV2-based VIGS system in pepper by testing the efficiency of <I>PDS</I> gene silencing under different conditions. This BBWV2-based vector system represents a convenient approach for rapid and simple analysis of gene functions in pepper.</P>