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Lee, Hye Kyoung,Choi, Sun-Hae,Lee, Cho Rong,Lee, Sun Hee,Park, Mi Ri,Kim, Younghoon,Lee, Myung-Ki,Kim, Geun-Bae Korean Society for Food Science of Animal Resource 2015 한국축산식품학회지 Vol.35 No.1
The present study was conducted to screen candidate probiotic strains for anti-inflammatory activity. Initially, a nitric oxide (NO) assay was used to test selected candidate probiotic strains for anti-inflammatory activity in cultures of the murine macrophage cell line, RAW 264.7. Then, the in vitro probiotic properties of the strains, including bile tolerance, acid resistance, and growth in skim milk media, were investigated. We also performed an in vitro hydrophobicity test and an intestinal adhesion assay using Caenorhabditis elegans as a surrogate in vivo model. From our screening, we obtained 4 probiotic candidate lactic acid bacteria (LAB) strains based on their anti-inflammatory activity in lipopolysaccharide (LPS)-stimulated RAW 264.7 cell cultures and the results of the in vitro and in vivo probiotic property assessments. Molecular characterization using 16S rDNA sequencing analysis identified the 4 LAB strains as Lactobacillus plantarum. The selected L. plantarum strains (CAU1054, CAU1055, CAU1064, and CAU1106) were found to possess desirable in vitro and in vivo probiotic properties, and these strains are good candidates for further investigations in animal models and human clinical studies to elucidate the mechanisms underlying their anti-inflammatory activities.
Lee, Dae Hong,Eun Jin Kang,Hee Jin Lee,Hee Bong Seok,Chang Wook Jo,Cho Rong Park,Myoung Gi Kang,Mi Ja Seo,Young Nam Youn 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.05
The multicolored Asian ladybird beetle, Harmonia axyridis, which demonstrates typical genetic polymorphism in its elytra color patterns. Early studies have color polymorphism in terms of geographical clines while a few investigated temporal populations in Coccinellidae. Nevertheless, note that geographical and temporal morph variation does not always correspond to what is expected from thermal and industrial adaption theories. A recent study of transformation and RNAi of the ladybird beetle, however, there is yet no evidence to indicate the variation is genetic or environmental factors. Here we describe a relatively new molecular fingerprinting technique, amplified fragment length polymorphism (AFLP). Because we think that color polymorphism in Coccinellidae is affected by genetic polymorphism. In total 38 markers were scored from which some markers were polymorphic. Supsequent UPGMA cluster analysis revealed 3 major group of Harmonia axyridis populations. But for strains that are more genetically similar, different primer combinations may be need to generate enough polymorphic marker.
Cho-Rong Lee,Kwang-Lai Park,Jeong-Lai Cho,Yura Oh,Sang-Min Lee,Nan-Hee An 한국토양비료학회 2020 한국토양비료학회지 Vol.53 No.2
Soil phosphate accumulation has been problems in some organic farmlands in Korea. This study was conducted to solubilize the accumulated phosphate in organic upland soils using green manure mixtures as substitute for chemical materials (EDTA etc.). We investigated the effects of barley-hairy vetch mixtures on the productivity of biomass and nutrient and the solubilization of soil phosphate. Treatments were barley single (B), hairy vetch single (H), mixtures of B and H at the ratio of 2:1 (B2H1), 1:1 (B1H1), 1:2 (B1H2), and control (CON). Green manures were cultivated on phosphate accumulated soils (>1,000 mg Lancaster P₂O₅ kg<SUP>-1</SUP>) for 3 years. Mixtures were more effective for stable production of yields and nutrient than B or H, because dry matter yields of green manures were more variable in B and H single. The quality of B as green manure improved in mixtures, because the C/N ratio of B in mixture increased less than B single. In addition, H was effective for phosphate solubilization, as water soluble phosphate contents of H single (2017 and 2019) and mixtures (2019) were significantly higher than B single (2017 and 2019) and CON (2019) despite of phosphate uptake by green manures. Therefore, barley-hairy vetch mixtures could be useful considering green manure productivity and phosphate solubilization in phosphate accumulated upland soils.
Lee, Seung-Yup,Lee, Donghwan,Nam, Cho Rong,Kim, Da Yea,Park, Sera,Kwon, Jun-Gun,Kweon, Yong-Sil,Lee, Youngjo,Kim, Dai Jin,Choi, Jung-Seok AKADEMIRI KIRDO 2018 JOURNAL OF BEHAVIOURAL ADDICTIONS Vol.7 No.2
<P><B>Background and objectives</B></P><P>The ubiquitous Internet connections by smartphones weakened the traditional boundaries between computers and mobile phones. We sought to explore whether smartphone-related problems differ from those of computer use according to gender using latent class analysis (LCA).</P><P><B>Methods</B></P><P>After informed consents, 555 Korean middle-school students completed surveys on gaming, Internet use, and smartphone usage patterns. They also completed various psychosocial instruments. LCA was performed for the whole group and by gender. In addition to ANOVA and χ<SUP>2</SUP> tests, post-hoc tests were conducted to examine differences among the LCA subgroups.</P><P><B>Results</B></P><P>In the whole group (<I>n</I> = 555), four subtypes were identified: dual-problem users (49.5%), problematic Internet users (7.7%), problematic smartphone users (32.1%), and “healthy” users (10.6%). Dual-problem users scored highest for addictive behaviors and other psychopathologies. The gender-stratified LCA revealed three subtypes for each gender. With dual-problem and healthy subgroup as common, problematic Internet subgroup was classified in the males, whereas problematic smartphone subgroup was classified in the females in the gender-stratified LCA. Thus, distinct patterns were observed according to gender with higher proportion of dual-problem present in males. While gaming was associated with problematic Internet use in males, aggression and impulsivity demonstrated associations with problematic smartphone use in females.</P><P><B>Conclusions</B></P><P>An increase in the number of digital media-related problems was associated with worse outcomes in various psychosocial scales. Gaming may play a crucial role in males solely displaying Internet-related problems. The heightened impulsivity and aggression seen in our female problematic smartphone users requires further research.</P>
Lee, Cho Rong,Kim, Changman,Song, Young Eun,Im, Hyeonsung,Oh, You-Kwan,Park, Sunghoon,Kim, Jung Rae Elsevier 2018 Bioresource technology Vol.259 No.-
<P><B>Abstract</B></P> <P>The biological conversion of carbon monoxide (CO) has been highlighted for the development of a C1 gas biorefinery process. Despite this, the toxicity and low reducing equivalent of CO uptake make biological conversion difficult. The use of synthetic co-cultures is an alternative way of enhancing the performance of CO bioconversion. This study evaluated a synthetic co-culture consisting of <I>Citrobacter amalonaticus</I> Y19 and <I>Sporomusa ovata</I> for acetate production from CO. In this consortium, the CO<SUB>2</SUB> and H<SUB>2</SUB> produced by the water-gas shift reaction of <I>C. amalonaticus</I> Y19, were utilized further by <I>S. ovata</I>. Higher acetate production was achieved in the co-culture system compared to the monoculture counterparts. Furthermore, syntrophic cooperation via various reducing equivalent carriers provided new insights into the synergistic metabolic benefits with a toxic and refractory substrate, such as CO. This study also suggests an appropriate model for examining the syntrophic interaction between microbial species in a mixed community.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Co-culture of <I>C. amalonaticus</I> Y19 and <I>S. ovata</I> was examined using CO as substrate. </LI> <LI> CO was converted to CO<SUB>2</SUB> and H<SUB>2</SUB> by Y19, then metabolized by ovata. </LI> <LI> Co-culture presented better CO consumption and acetate production than mono-culture. </LI> <LI> The addition of HNQ as mediator improves conversion of CO to acetate. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Cho-Rong Lee,Yura Oh,Jung A Jung,Sang-Min Lee,Nan-Hee An 한국토양비료학회 2020 한국토양비료학회지 Vol.53 No.4
Nitrogen (N) supply from organic materials (OM) takes more time than chemical fertilizer (CF), because mineralization of organic N requires microbial conversion. Most organic N applied to soil remains into SOM. It might have a greater residual effect than CF on soil fertility and crop nutrient uptake and influence on N fate. Residual organic N might have influence on soil fertility and nutrient uptake of crop by depending on N cycling in soil. This study investigated the residual effect of <SUP>15</SUP>N-labeled organic materials on Chinese cabbage N uptake and soil N. <SUP>15</SUP>N-labeled organic materials at rate of 456 ㎏ N/ha applied with green manure (GM), livestock compost (LC), GM+LC, oil cake (OC), and CF into wagner pot (1/2000a) for Chinese cabbage cultivation. During continuous crop cultivation (Sep. 2018 to Mar, 2020), <SUP>15</SUP>N-labeled Chinese cabbage and soil was analyzed by using stable isotope elemental analyzer. Organic materials and chemical fertilizer were higher N uptake in Chinese cabbage than in the control at first cropping season. However, N uptake of Chinese cabbage under continuous cultivation was significantly increased 35-72% at organic material treatments compared to the control. Also, <SUP>15</SUP>N-labeled soil was significantly high at the treatments applied with GM, LC, and GM+LC, but was not at OC and CF treatment. In conclusion, organic materials (especially GM, LC, and GM+LC) had a greater N residual effect on Chinese cabbage and soil than chemical fertilizer.
Cho-Rong Lee,Yura Oh,Kwang-Lai Park,Jeong-Lai Cho,Nan-Hee An,Sang-Min Lee 한국토양비료학회 2020 한국토양비료학회지 Vol.53 No.3
Phosphorus (P) easily accumulates in farmland soil due to their high affinity to cations and clay, and thus their use efficiency of crop is low. Green manure-maize rotation system might be an efficient measure to reduce P accumulation in organic farmland by improving P uptake of crops. This study was conducted to investigatethe effects of green manure-maize rotation on P uptake of maize and excess P removal in organic upland soils. Maize mono cropping and green manure-maize rotation systems (barley (B), hairy vetch (H), mixture of B and H) were established in organic farmland, and the experiments were conducted for 2 years without additional nutrients application. Green manure had a significant impact on maize grain production. Maize P uptake increased with the increased rate of hairy vetch seeding. Water soluble P content in soil was further reduced in green manure-maize rotation systems compared to the mono cropping. The results suggest that green manure-maize rotation system (except barley) without nutrient supplement increases P uptake of maizeand thus decreases soil P accumulation.