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Cho, Myoung-Lae,Yang, Chen,Kim, Sang-Moo,You, Sang-Guan 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.2
Sulfated polysaccharides isolated from Enteromorpha prolifera and fractionated using ion exchange and gel permeation chromatography were investigated to determine their molecular characteristics and biological activities. The crude and fractionated polysaccharides ($F_1$, $F_2$, and $F_3$) consisted mostly of carbohydrates (50.8-62.5%), sulfates (14.5-18.8%), and uronic acid (13.8-16.6%) with different levels of monosaccharides, including rhamnose (57.1-87.6%), glucose (3.6-39.1%), and xylose (2.4-8.8%). The protein content ranged from 1.0 to 13.9 %, and after the fractionation, most proteins were included in the F2 fraction (11.3%). The crude, $F_1$, $F_2$, and $F_3$ exhibited the molecular weights (Mw) of $37-1,281{\times}10^3\;g/mol$. The polysaccharides had no significant direct cytotoxicity to cancer cells AGS or DLD-1. On the other hand, the $F_1$ and $F_2$ stimulated Raw 264.7 cells to produce a considerable amount of nitric oxide (NO). The investigation of $F_2$ subfractions failed to reveal a specific subfraction more significantly affecting the NO production, thus requiring further systematic elucidation of their structural characteristics.
( Myoung Lae Cho ),( Gab Man Park ),( Su Nam Kim ),( Touseef Amna ),( Seok Joon Lee ),( Woon Seob Shin ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.3
The chlorophyll-related compound pheophorbide a (Pa) was successively purified from an edible red seaweed, Grateloupia elliptica, using silica, octadecyl silica column chromatography and reversed phase-high-performance liquid chromatography, as well as the cell cycle inhibitory and apoptotic effects of Pa being investigated in U87MG glioblastoma cells. The Pa exhibited strong anticancer effects in the absence of direct photo-irradiation against various cancer cell lines, including U87MG, SK-OV-3, and HeLa cells. Among the cancer cells, the strongest anticancer activity of Pa exhibited on U87MG cells with IC50 values of 2.8μg/ml. In addition, Pa specifically had cytostatic activity on glioblastoma cells rather than human umbilical vein endothelial cells. Analysis of the cell cycle distribution showed that Pa induced G0/G1 arrest of U87 MG cells. In addition, arrested cells induced late apoptosis and DNA degradation under dark condition. These results suggest that Pa isolated from G. elliptica is a potential glioblastoma-specific anticancer agent without side effects on normal cells.
가공조건에 따른 사철쑥 침출차의 품질 특성 및 항산화 활성 연구
조명래(Myoung Lae Cho),이종석(Jong Seok Lee),김민경(Min-Kyung Kim),신혜영(Hye-Young Shin),이사라(Sarah Lee),김병직(Byung-Jik Kim),여주홍(Joohong Yeo),김종예(Jong-Yea Kim) 한국식품영양과학회 2018 한국식품영양과학회지 Vol.47 No.6
본 연구에서는 사철쑥을 침출차로 활용하고자 에탄올을 이용하여 사철쑥의 쓴맛을 제거하였으며, 열처리를 이용하여 사철쑥의 풍미를 향상시켰다. 관능평가 결과 아무것도 처리하지 않은 사철쑥으로 침출차를 제조한 경우 쑥 특유의 쓴맛이 강하여 전체적인 기호도가 감소하였다. 하지만 50% 에탄올 처리에 의해서 사철쑥 특유의 쓴맛을 제거할 수 있으며, 160°C의 열처리를 통하여 사철쑥의 풍미를 증가시킬 수 있었다. 또한, 에탄올 처리와 열처리를 병행했을 때 사철쑥 특유의 쓴맛을 가장 많이 감소시킬 수 있었고, 총 페놀릭 성분함량은 약간 감소하였지만 침출차의 전반적인 항산화력은 유지되었다. 따라서 본 연구 결과 사철쑥 침출차 제조 시 에탄올 처리와 열처리를 병행한다면 사철쑥 특유의 쓴맛을 제거하고 풍미를 향상시킬 수 있는 침출차를 제조할 수 있을 것이라 기대된다. Artemisia capillaris was subjected to an ethanol treatment (50 and 95%) and/or heat treatment (100, 120, 140, 160, 180, and 200°C) to enhance the overall quality of leached A. capillaris beverage. Both ethanol and heat treatments did not induce obvious changes in the 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity regardless of the treatment conditions. The ethanol treatment (50 and 95%) reduced the flavor and bitter taste of the A. capillaris beverage but increased the overall preference score. As a heat treatment, when the temperature was increased to 160°C, both the flavor and bitterness scores of A. capillaris beverage were decreased but the roasted favor and overall preference scores were increased. A combination of both ethanol treatment (50%) and heat treatment (160°C) also reduced the bitter taste of the beverage but the overall preference score was also decreased slightly regardless of the treatment time.
김재민(Jae-Min Kim),조명래(Myoung-Lae Cho),서규은(Kyu-Eun Seo),김예슬(Ye-Seul Kim),정태동(Tae-Dong Jung),김영현(Young-Hyun Kim),김단비(Dan-Bi Kim),신기해(Gi-Hae Shin),오지원(Ji-Won Oh),이종석(Jong Seok Lee),이진하(Jin-Ha Lee),김종예(Jon 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.8
본 연구는 유근피를 항산화 소재로 사용하기 위한 기초 자료를 제공하고자 에탄올의 농도(0, 40, 80% 에탄올) 및 추출시간(1, 2, 3시간)이 다른 조건에서 추출하였으며, 이들 추출물의 총 폴리페놀 및 플라보노이드 함량을 측정하였다. 또한 다양한 추출물의 DPPH 라디칼 소거능, ABTS 라디칼 소거능, 환원력, ORAC value 등의 항산화 활성을 측정하였으며, 총 폴리페놀 및 플라보노이드 함량과 항산화 활성과의 연관성을 분석하였다. 그 결과 유근피 추출물은 80% 에탄올로 3시간 추출하였을 때 가장 높은 총 폴리페놀 및 플라보노이드 함량과 항산화 활성을 보였다. 또한 DPPH 및 ABTS 라디칼 소거능, 환원력은 에탄올 함량에 따른 활성 차이는 보였으나 추출시간에 따른 항산화 활성 차이는 보이지 않았다. 하지만 ORAC는 유근피 추출물의 추출시간이 증가함에 따라 함께 증가하였다. 이상의 결과로 볼 때 유근피 추출물은 80% 에탄올을 이용하여 70°C에서 3시간 추출을 할 때 항산화 활성을 갖는 phenolic 및 flavonoid 계열의 유효성분이 가장 많이 추출되었다. 또한 유근피 80% 에탄올 3시간 추출물은 피부 섬유아세포와 3T3-L1 지방세포에서 세포독성을 나타내지 않았고 피부 섬유아세포에서 산화적 스트레스에 대한 세포 보호 효과를 가졌으며, 3T3-L1 지방세포에서 활성산소종 생성량을 감소하였다. 따라서 높은 항산화력을 가진 유근피 추출물은 천연 항산화제로 충분히 사용 가능할 것으로 사료된다. This study investigated optimal extraction conditions for application of Ulmus pumila L. as a natural antioxidant. U. pumila L. was extracted using ethanol (EtOH) at various concentrations (0, 40, and 80%) and extraction times (1, 2, and 3 h) at 70°C and then evaluated for extraction yield, total phenolic contents, total flavonoid contents, as well as antioxidant activities [2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2"-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, reducing power, and oxygen radical absorbing capacity (ORAC)]. Antioxidant activities were correlated with total phenolic and flavonoid contents. Of the solvent conditions, 80% EtOH extracts for 3 h at 70°C showed the highest total phenolic and flavonoid contents with strong antioxidant activities, although there were no significant time effects on DPPH and ABTS radical scavenging activities and reducing power. However, ORAC values of all EtOH extracts remarkably increased in a time-dependent manner. In addition, 80% EtOH extract for 3 h exhibited strong antioxidant effects on HDF and 3T3-L1 cells. Therefore, the antioxidant capacity of U. pumila L., may due to phenolic and flavonoid contents, and extraction conditions were 80% EtOH for 3 h at 70°C. This extract could be a good source for natural antioxidants.
생물전환에 의한 발효 고려엉겅퀴 Pectolinarin 및 Pectolinarigenin의 분석법 개발 및 검증
오지원(Ji-Won Oh),이진하(Jin-Ha Lee),조명래(Myoung-Lae Cho),신기해(Gi-Hae Shin),김재민(Jae-Min Kim),최선일(Sun-Il Choi),정태동(Tae-Dong Jung),김영현(Young-Hyun Kim),이상종(Sang-Jong Lee),이봉진(Bong Jin Lee),박선주(Seon Ju Park),이옥환( 한국식품영양과학회 2015 한국식품영양과학회지 Vol.44 No.10
생물전환에 의한 고려엉겅퀴 pectolinarin의 pectolinarigenin 전환 시 지표성분의 함량 및 표준화를 위한 분석법 검증을 실시하였다. 분석법 검증 결과 HPLC를 이용한 분석방법에서 표준용액의 retention time과 발효 고려엉겅퀴 추출물의 retention time이 일치하였으며 동일한 spectrum을 나타내는 것으로 특이성을 확인하였다. Pectolinarin 및 pectolinarigenin의 검량선은 상관계수 값이 모두 1로 나타나 높은 직선성을 보여주어 분석에 적합함을 알 수 있었다. Pectolinarin 및 pectolinarigenin의 검출한계는 각각 4.25 μg/mL, 2.46 μg/mL였고, 정량한계는 12.88 μg/mL, 7.46 μg/mL로 나타났다. Pectolinarin 및 pectolinarigenin의 함량을 확인한 결과 고려엉겅퀴 0시간, 5시간 발효물에서는 pectolinarin만 관찰되었으며, 19시간 발효물에서 생물전환에 의해 pectolinarin이 pectolinarigenin으로 전환되는 것을 확인할 수 있었다. 그 이후 28시간부터 72시간까지의 고려엉겅퀴 발효물에서는 pectolinarigenin만 관찰할 수 있었다. 정밀도 측정 결과 pectolinarin 및 pectolinarigenin은 일간 정밀도에서 각각 0.9%, 0.5%의 정밀도를 보여주었으며 일내 정밀도에서는 각각 0.5%, 0.2%로 높은 정밀성을 나타내었다. 또한 pectolinarin은 99.7~104.0%, pectolinarigenin은 99.7~102.4% 범위의 회수율을 보여주어 실험방법에 대한 정확성을 검증하였다. 본 연구 결과 고려엉겅퀴의 지표성분인 pectolinarin 및 pectolinarigenin의 HPLC를 이용한 동시분석방법이 적합한 분석방법임이 검증되었다. The aim of this study was to investigate a validation method for determination of pectolinarin and pectolinarigenin in fermented Cirsium setidens Nakai. For validation, the specificity, linearity, precision, accuracy, detection limits, and quantification limits of pectolinarin and pectolinarigenin were measured by HPLC. The results show that the detection limits of pectolinarin and pectolinarigenin were 4.25 μg/mL and 2.46 μg/mL, respectively. The recovery rates of pectolinarin and pectolinarigenin were high in the ranges of 99.7∼104.0% and 99.7∼102.4%, respectively. Inter-day and intra-day precisions of pectolinarin and pectolinarigenin in fermented Cirsium setidens Nakai were 0.9%, 0.5% and 0.5%, 0.2%, respectively. Therefore, application of pectolinarin and pectolinarigenin was validated by an analytical method as a marker compound in Cirsium setidens Nakai.
Selective inhibition of monoamine oxidase A by chelerythrine, an isoquinoline alkaloid
Baek, Seung Cheol,Ryu, Hyung Won,Kang, Myung-Gyun,Lee, Hanna,Park, Daeui,Cho, Myoung-Lae,Oh, Sei-Ryang,Kim, Hoon Elsevier 2018 Bioorganic & medicinal chemistry letters Vol.28 No.14
<P><B>Abstract</B></P> <P>Chelerythrine, an isoquinoline alkaloid isolated from the herbaceous perennial <I>Chelidonium majus</I>, was found to potently and selectively inhibit an isoform of recombinant human monoamine oxidase-A (MAO-A) with an IC<SUB>50</SUB> value of 0.55 µM. Chelerythrine was a reversible competitive MAO-A inhibitor (K<SUB>i</SUB> = 0.22 µM) with a potency much greater than toloxatone (IC<SUB>50</SUB> = 1.10 µM), a marketed drug. Other isoquinoline alkaloids tested did not effectively inhibit MAO-A or MAO-B. A structural comparison with corynoline suggested the 1- and/or 2-methoxy groups of chelerythrine increase its inhibitory activity against MAO-A. Molecular docking simulations revealed that the binding affinity of chelerythrine for MAO-A (−9.7 kcal/mol) was greater than that for MAO-B (−4.6 kcal/mol). Docking simulation implied that Cys323 and Tyr444 of MAO-A are key residues for hydrogen-bond interaction with chelerythrine. Our findings suggest chelerythrine is one of the most reversible selective and potent natural inhibitor of MAO-A, and that it be regarded a potential lead compound for the design of novel reversible MAO-A inhibitors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Chelerythrine was a potent (IC<SUB>50</SUB> = 0.55 µM) and selective inhibitor for human MAO-A. </LI> <LI> Chelerythrine was a reversible and competitive inhibitor (K<SUB>i</SUB> = 0.22 µM). </LI> <LI> The binding affinity of chelerythrine for MAO-A was greater than that for MAO-B. </LI> <LI> The 1-methoxy groups of chelerythrine increase its inhibitory activity against MAO-A. </LI> <LI> Chelerythrine can be a potential lead compound for design novel reversible MAO-A inhibitors. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>