http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Chi Heung Cho ),( Holim Jang ),( Migi Lee ),( Hee Kang ),( Ho Jin Heo ),( Dae-ok Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.7
The present study was carried out to investigate the antioxidative and neuroprotective effects of sea buckthorn (Hippophae rhamnoides L.) leaves (SBL) harvested at different times. Reversed-phase high-performance liquid chromatography analysis revealed five major phenolic compounds: ellagic acid, gallic acid, isorhamnetin, kaempferol, and quercetin. SBL harvested in August had the highest total phenolic and flavonoid contents and antioxidant capacity. Treatment of neuronal PC-12 cells with the ethyl acetate fraction of SBL harvested in August increased their viability and membrane integrity and reduced intracellular oxidative stress in a dose-dependent manner. The relative populations of both early and late apoptotic PC-12 cells were decreased by treatment with the SBL ethyl acetate fraction, based on flow cytometry analysis using annexin V-FITC/PI staining. These findings suggest that SBL can serve as a good source of antioxidants and medicinal agents that attenuate oxidative stress.
녹용의 무기질 조성, 항산화능 및 PC-12 신경 세포 보호능에 대한 급여 사료의 영향
조치홍(Chi Heung Cho),이봉한(Bong Han Lee),김해영(Hae-Yeong Kim),김영채(Young Chae Kim),김대옥(Dae-Ok Kim) 한국생물공학회 2012 KSBB Journal Vol.27 No.4
The ash content, mineral composition, total phenolics, antioxidant capacity, and neuroprotective effect of the antlers of deer fed with herb-incorporated feedstuff (HFS) or normal feedstuff (NFS) were comparatively evaluated. The contents of ash and mineral of the antler of deer fed with HFS were significantly lower than its counterpart. The ash and mineral contents of deer antlers decreased generally from the upper section toward the tip section. The ratios of Ca/ash, Ca/P, and Ca/Fe of antler of deer fed with HFS were lower than those of antler of deer fed with NFS. Antlers of deer fed with HFS had higher total phenolics, nitrite scavenging capacity, and antioxidant capacity than those of deer fed with NFS. Antlers of deer fed with HFS or NFS showed in vitro neuronal protection of PC-12 cells against oxidative stress in a dosedependent manner, where antler of deer fed with HFS generally had higher cellular viability than NFS. These results above suggest that the incorporation of the medicinal herbal complex into feedstuff may improve the biological effects of deer antlers.
우뭇가사리(Gelidium amansii)추출물의 Mouse Glomerular Mesangial Cells에최종당화산물로 유도된 당뇨병성 신병증 완화 효과
조치흥 ( Chi Heung Cho ),김민경 ( Mingyeong Kim ),김세라 ( Sera Kim ),이상훈 ( Sang-hoon Lee ) 한국키틴키토산학회 2021 한국키틴키토산학회지 Vol.26 No.4
본 연구에서는 제주도에서 자생하는 홍조류인 우뭇가사리 (Gelidium amansii) 70% (v/v) 에탄올 추출물(GAE)의 항당화 (anti-glycation) 효능 및 최종당화산물로 유도되는 당뇨병성 합병증 중 하나인 신장병증(nephropathy)에 대한 예방 효과를 평가하였다. GAE는 모든 처리 농도(1, 5, 및 20 μg/mL)에서 BSA, glucose, 및 fructose로 인해 생성되는 최종당화산물을 유의적으로 감소시키는 것을 확인하였다. 또한 GAE의 전처리는 최종당화산물과 콜라겐 단백질 사이의 교차결합 형성을 유의적으로 억제 시켰으며, 최종당화산물과 콜라겐 단백질 사이에 이미 형성된 비가역적 교차결합에 GAE를 처리하였을 때 매우 효과적으로 절단하는 효능을 가지고 있음을 확인하였다. GAE의 신장 보호 효과를 평가하기 위해 mouse glomerular mesangial 세포 보호 효능을 평가한 결과 최종당화산물의 전구체인 MGO로 유도된 세포 독성으로부터 신장 세포를 보호하는 효과를 나타내었다. 이러한 효과는 GAE의 전처리가 MGO로 인해 증가하는 신장 세포 내 ROS 생성 억제, 세포 내 최종당화산물 축적 감소, 및 세포 내 MGO 축적 감소를 통해 신장 세포의 apoptosis를 억제하기 때문인 것을 확인하였다. 본 연구를 통해 GAE는 합성 당뇨합병증 치료제로 알려진 아 미노구아니딘과 유사한 수준의 효능을 가지고 있음을 확인하였다. 그러므로 우뭇가사리는 최종당화산물로 인해 유도되는 당뇨병성 신병증을 예방 및 치료하는 효과를 가지고 있는 것으로 보이며, 부작용 위험이 낮은 천연 당뇨합병증 치료제로 개발 잠재력이 높은 것으로 사료된다. In this study, we evaluated the anti-glycation activity and nephroprotective effects of Gelidium amansii 70% (v/v) ethanol extract (GAE) in mouse glomerular mesangial cells. GAE (1, 5, and 20 μg/mL) treatment significantly reduced advanced glycation end-products (AGEs) formation (p < 0.001), and inhibited AGEs formation up to 47.8% at 1 μg/mL GAE. In addition, GAE not only significantly inhibited the formation of AGEs-collagen cross-linking, but also showed the effect of breaking the already formed cross-links (p < 0.001). In particular, 20 μg/mL GAE (41.9%) exhibited similar breaking ability as ALT-711 (0.5 mg/mL, 33.7%) used as a positive control. Treatment of methylglyoxal (MGO), a precursor of AGEs, on mouse glomerular mesangial cells, increased intracellular MGO and AGEs accumulation, leading to increased reactive oxygen species (ROS) production and eventually induced renal cell apoptotic death. However, pre-treatment with the GAE, at 1, 5 and 20 μg/mL, reduced intracellular ROS production level to approximately 541.1-201.4%. Especially, pre-treatment of 20 μg/mL GAE significantly decreased intracellular MGO concentration (18.99 μg/mL) to a level similar to that of the non-treated group (16.67 μg/mL) (p < 0.001). Moreover, it was confirmed by immunofluorescence analysis that GAE suppressed the AGEs accumulation in mouse glomerular mesangial cells. As a result, due to this effects of GAE, MGO-induced apoptotic cell death was dramatically reduced, proving that GAE has an ability in preventing AGEs-related diabetic nephropathy. Therefore, our results suggest that Gelidium amansii has high potential to be developed as a natural agent for diabetic complications with a low risk of side effects.
넓패(Ishige foliacea)의 최종당화산물 생성저해 활성 및 신장세포 보호 효과
조치흥 ( Chi Heung Cho ),김민경 ( Mingyeong Kim ),염가희 ( Ga Hee Youm ),김세라 ( Sera Kim ),박용곤 ( Yongkon Park ),이상훈 ( Sang-hoon Lee ) 한국키틴키토산학회 2020 한국키틴키토산학회지 Vol.25 No.3
본 연구에서 국내 자생 갈조류 중 하나인 넓패를 이용하여 추출방법에 따른 생리활성 물질의 함량을 정량적으로 분석하였다. 또한 당뇨병성신장증의 합병증을 유발하는 물질로 알려진 최종당화산물의 생성 억제 효과, 최종당화산물과 콜라겐 사이의 교차결합 형성 억제 효과 및 교차결합 절단 효과를 평가하였다. 넓패 70% 에탄올 추출물은 총 페놀, 총 플라보노이드 및 탄닌과 같은 생리활성 성분을 가장 많이 함유하는 추출 조건임을 확인하였으며, 최종당화산물의 생성 억제, 최종당화산물과 콜라겐 사이의 가교결합 형성 저해 및 생성된 가교결합을 절단하는 효과 또한 가장 높았다. 특히, 70% EtOH IFE는 양성대조군으로 처리한 AG보다 더 높은 교차 결합 억제 및 교차결합 절단 효과를 나타내는 것을 확인할 수 있었다. 또한 70% EtOH IFE는 MGO에 의해 유도되는 산화적 스트레스로부터 신장 세포인 mesangial 세포의 세포 내 산화적 스트레스 수준을 유의적으로 감소시킬 뿐만 아니라 apoptosis로 인한 세포 사멸을 감소시켜 신장 손상을 보호하는 효과를 보였다. 따라서 넓패는 부작용이 적은 당뇨합병증 예방 및 치료제 개발 후보물질로서 가능성이 충분한 것으로 판단되며, 향후 국내 자생 해양자원의 가치 향상을 위해 넓패의 생리활성 성분의 동정 및 당뇨합병증 예방과 치료에 작용하는 메커니즘 구명 연구가 추가적으로 진행되어야 할 것으로 사료된다. The present study was conducted to investigate the anti-glycation activities and renoprotective properties of Ishige foliacea. The yield of extract ranged from 3.20 to 35.59%. Among the extracts, 70% aqueous ethanol extract of Ishige foliacea (70% EtOH IFE) showed the highest total phenolic, condensed tannin, and total flavonoid contents (1.570 ± 0.165 mg GAE/g DW, 0.407 ± 0.008 mg CE/g DE, 0.353 ± 0.050 mg CE/g DE, respectively). 70% EtOH IFE showed the highest breaking activity against preformed AGEs-BSA cross-link than ALT-711, the well-known cross-link breaker. In addition, 70% EtOH IFE observedthe highest inhibitory effect on the AGEs-BSA cross-link and exhibited more potent than aminoguanidine, a well-known AGEs-BSA cross-link inhibitor. Treatment with the 70% EtOH IFE, at 2, 10, and 50 μg/mL, reduced oxidative stress to the level of approximately 186.3-95.9%. The intracellular antioxidative effect of the 70% EtOH IFE was stronger to that with amino-guanidine at 500 μM. Pre-treatments of cells with the 70% EtOH IFE at concentrations of 2, 10, and 50 μg/mL led to 60.3%, 38.0%, and 20.8% decrease in apoptotic cells, respectively. These result suggest that 70% aqueous ethanol extract of Ishige foliacea can serve as a good source of natural anti-diabetic complications agents via breaking and inhibition of AGEs-BSA cross-link and renoprotective effects against MGO-induced oxidative stress.
폐경 후 호르몬 요법시 초기 생화학적 골표지자와 골밀도 변화와의 상관관계
조지희,공은희,최영식,김양수,김흥열,어완규,홍기연 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.1
Background : This study was aimed to estimate the relationship between bone mineral density after hormone therapy (HT) for one year and short-term changes of bone markers in postmenopausal women. Methods : Biochemical markers of serum osteocalcin (OC) and urine deoxypyridinoline (Dpd) at 3 months and lumbar bone mineral density (BMD) were measured at 1 year in 80 operated postmenopausal women(PMW) taking HT. Results : Serum OC and urine Dpd decreased from 10.85% at baseline to 7.87% at 3 months and BMD increased 2.2% after 1 year of treatment. 56 (80%) among 70 with decreased OC revealed increasement of BMD, while 50 (78.1%) among 64 PMW with decreased urine Dpd showed increasement of BMD. Serum OC of bone gainers was lower than of bone losers (10.18% vs 14.01%) and urine Dpd also lower than that of bone losers (7.29% vs 10.6%). No correlations between biochemical markers such as, serum OC (r=-0.034) and urine Dpd (r=-0.031that ) checked at 3 months and BMD at 1 year were seen in postmenopausal women. Conclusion : Short-term changes of bone markers did not precisely predict the long-term changes of BMD in postmenopausal women.