Visualizing the distributions and spatiotemporal changes of metabolites in Panax notoginseng by MALDI mass spectrometry imaging

Chenglong Sun,Shuangshuang Ma,Lili Li,Daijie Wang,Wei Liu,Feng Liu,Lanping Guo,Xiao Wang 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.6

Background: Panax notoginseng is a highly valued medicinal herb used widely in China and many Asiancountries. Its root and rhizome have long been used for the treatment of cardiovascular and hematologicaldiseases. Imaging the spatial distributions and dynamics of metabolites in heterogeneous planttissues is significant for characterizing the metabolic networks of Panax notoginseng, and this will alsoprovide a highly informative approach to understand the complex molecular changes in the processing ofPanax notoginseng. Methods: Here, a high-sensitive MALDI-MS imaging method was developed and adopted to visualize thespatial distributions and spatiotemporal changes of metabolites in different botanical parts of Panaxnotoginseng. Results: A wide spectrum of metabolites including notoginsenosides, ginsenosides, amino acids, dencichine,gluconic acid, and low-molecular-weight organic acids were imaged in Panax notoginseng rhizomeand root tissues for the first time. Moreover, the spatiotemporal alterations of metabolites during thesteaming of Panax notoginseng root were also characterized in this study. And, a series of metabolitessuch as dencichine, arginine and glutamine that changed with the steaming of Panax notoginseng weresuccessfully screened out and imaged. Conclusion: These spatially-resolved metabolite data not only enhance our understanding of the Panaxnotoginseng metabolic networks, but also provide dire

Thermal-hydraulic analysis of a new conceptual heat pipe cooled small nuclear reactor system

Wang, Chenglong,Sun, Hao,Tang, Simiao,Tian, Wenxi,Qiu, Suizheng,Su, Guanghui Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.1

Small nuclear reactor features higher power capacity, longer operation life than conventional power sources. It could be an ideal alternative of existing power source applied for special equipment for terrestrial or underwater missions. In this paper, a 25kWe heat pipe cooled reactor power source applied for multiple use is preliminary designed. Based on the design, a thermal-hydraulic analysis code for heat pipe cooled reactor is developed to analyze steady and transient performance of the designed nuclear reactor. For reactor design, UN fuel with 65% enrichment and potassium heat pipes are adopted in the reactor core. Tungsten and LiH are adopted as radiation shield on both sides of the reactor core. The reactor is controlled by 6 control drums with B₄C neutron absorbers. Thermoelectric generator (TEG) converts fission heat into electricity. Cooling water removes waste heat out of the reactor. The thermal-hydraulic characteristics of heat pipes are simulated using thermal resistance network method. Thermal parameters of steady and transient conditions, such as the temperature distribution of every key components are obtained. Then the postulated reactor accidents for heat pipe cooled reactor, including power variation, single heat pipe failure and cooling channel blockage, are analyzed and evaluated. Results show that all the designed parameters satisfy the safety requirements. This work could provide reference to the design and application of the heat pipe cooled nuclear power source.

Ginsenoside Re inhibits myocardial fibrosis by regulating miR-489/myd88/NF-κB pathway

Jinghui Sun,Ru Wang,Tiantian Chao,Jun Peng,Chenglong Wang,Keji Chen The Korean Society of Ginseng 2023 Journal of Ginseng Research Vol.47 No.2

Background: Myocardial fibrosis (MF) is an advanced pathological manifestation of many cardiovascular diseases, which can induce heart failure and malignant arrhythmias. However, the current treatment of MF lacks specific drugs. Ginsenoside Re has anti-MF effect in rat, but its mechanism is still not clear. Therefore, we investigated the anti-MF effect of ginsenoside Re by constructing mouse acute myocardial infarction (AMI) model and AngII induced cardiac fibroblasts (CFs) model. Methods: The anti-MF effect of miR-489 was investigated by transfection of miR-489 mimic and inhibitor in CFs. Effect of ginsenoside Re on MF and its related mechanisms were investigated by ultrasonographic, ELISA, histopathologic staining, transwell test, immunofluorescence, Western blot and qPCR in the mouse model of AMI and the AngII-induced CFs model. Results: MiR-489 decreased the expression of α-SMA, collagenI, collagen III and myd88, and inhibited the phosphorylation of NF-κB p65 in normal CFs and CFs treated with AngII. Ginsenoside Re could improve cardiac function, inhibit collagen deposition and CFs migration, promote the transcription of miR-489, and reduce the expression of myd88 and the phosphorylation of NF-κB p65. Conclusion: MiR-489 can effectively inhibit the pathological process of MF, and the mechanism is at least partly related to the regulation of myd88/NF-κB pathway. Ginsenoside Re can ameliorate AMI and AngII induced MF, and the mechanism is at least partially related to the regulation of miR-489/myd88/NF-κB signaling pathway. Therefore, miR-489 may be a potential target of anti-MF and ginsenoside Re may be an effective drug for the treatment of MF.

The effect of biogas slurry application on biomass production and the silage quality of corn

Hua Sun,Kai Shi,Hairong Ding,Chenglong Ding,Zhiqing Yang,Chen An,Chongfu Jin,Beiyi Liu,Zhaoxin Zhong,Xia Xiao,Fuyin Hou Asian Australasian Association of Animal Productio 2023 Animal Bioscience Vol.36 No.12

Objective: The objective of this study was to evaluate the effect of biogas slurry application on biomass production and the silage quality of corn. Methods: A field experiment was conducted in which corn was grown using different biogas slurry application rates. The effect of 25% to 500% biogas slurry nitrogen replacement (T1 to T14) on the yield and quality indices of corn were studied by field plot experiments. Results: The results revealed that biogas slurry application improved the stem diameter and relative feed value of corn silage in treatments T13 and T11. Moreover, the fermentation quality of corn silage was improved due to an increase in lactic acid content; in comparison with the chemical synthetic fertilizer (CF) group. The crude protein contents of corn silage had no obvious change with increasing biogas slurry application. However, the forage quality index of acid detergent fiber was decreased (p<0.05) in the T11 group compared with the CF group. In addition, higher (p<0.05) 30 h in vitro dry matter digestibility and 30 h in vitro neutral detergent fiber digestibility were observed in the T11 and T13 groups than in the CF group. Conclusion: Based on these results, it was concluded that the optimum biogas slurry application rate for corn was approximately 350% to 450% biogas slurry nitrogen replacement under the present experimental conditions.

Preliminary design and assessment of a heat pipe residual heat removal system for the reactor driven subcritical facility

Wenwen Zhang,Kaichao Sun,Chenglong Wang,Dalin Zhang,Wenxi Tian,Suizheng Qiu,G.H. Su 한국원자력학회 2021 Nuclear Engineering and Technology Vol.53 No.12

A heat pipe residual heat removal system is proposed to be incorporated into the reactor drivensubcritical (RDS) facility, which has been proposed by MIT Nuclear Reactor Laboratory for testing anddemonstrating the Fluoride-salt-cooled High-temperature Reactor (FHR). It aims to reduce the risk of thesystem operation after the shutdown of the facility. One of the main components of the system is an aircooled heat pipe heat exchanger. The alkali-metal high-temperature heat pipe was designed to meet theoperation temperature and residual heat removal requirement of the facility. The heat pipe modeldeveloped in the previous work was adopted to simulate the designed heat pipe and assess the heattransport capability. 3D numerical simulation of the subcritical facility active zone was performed by thecommercial CFD software STAR CCM þ to investigate the operation characteristics of this proposedsystem. The thermal resistance network of the heat pipe was built and incorporated into the CFD model. The nominal condition, partial loss of air flow accident and partial heat pipe failure accident weresimulated and analyzed. The results show that the residual heat removal system can provide sufficientcooling of the subcritical facility with a remarkable safety margin. The heat pipe can work under therecommended operation temperature range and the heat flux is below all thermal limits. The facilitypeak temperature is also lower than the safety limits

Network pharmacology prediction to discover the potential pharmacological action mechanism of Rhizoma Dioscoreae for liver regeneration

Wei Liu,Wenyu Wang,Chenglong Tian,Ming-Zhong Sun,Shuqing Liu,Qinlong Liu 대한생리학회-대한약리학회 2024 The Korean Journal of Physiology & Pharmacology Vol.28 No.5

Magnetic Field-Driven and Photocured Rapid Shaping of Carbonyl Iron Particles in the Coating to Achieve Superhydrophobic Fabric

Duchao Feng,Luyang Qiao,Guangdong Sun,Kangjing Hong,Mingyue Wu,Chenglong Wang,Jinhuan Zheng 한국섬유공학회 2023 Fibers and polymers Vol.24 No.10

A magnetic field-driven photocurable superhydrophobic fabric coating technology was developed in this study. The coating, consisting of photoreactive carbonyl iron powder (CIP) assembled hydrophobic micro-nano structures, was constructed on the surface of polyester fabric by combining the magnetic field-driven assembly technology and blue light photocuring technology. Camphorquinone (CQ), ethyl 4-dimethylaminobenzoate (EDB) and diphenyliodonium hexafluorophosphate (DPI) were served as the photoinitiator formulation, while polyurethane diacrylate and hydroxyethyl acrylate (HEA) were used as the polymerization-cross-linking matrix for photocurable coating. Photo-differential scanning calorimetry (Photo-DSC) and rheometry were used to characterize the performance of coating formulations. Optical contact angle meter and scanning electron microscope (SEM) were used in combination with tensile performance test to explore the construction of superhydrophobic structure on coating surface induced by magnetic field. The optimal formulation of photoinitiators for coatings with excellent photopolymerization performance has been definitively established. Photocured coating film displayed a distinct hydrophobicity (the water contact angle is approx. 152.7°, and the sliding angle is approx. 7.2°) when the mass ratio of CIP to polymeric components was 1:1. The coated fabric eventually exhibited a satisfactory hydrophobicity (the water contact angle is approx. 153.3°) and mechanical properties.