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( Chang Bo Dai ),( Myeong Hyeon Wang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.10
To investigate the pathways of oxidoreductases in plants, 2 key components in thioredox systems i.e. thioredoxin h (Trx h) and NADPH-dependent thioredoxin reductase (NTR) genes were first isolated from tomatoes (Solanum lycopersicum). Subsequently, the coding sequences of Trx h and NTR were inserted into pET expression vectors, and overexpressed in Escherichia coli. In the UV-Visible spectra of the purified proteins, tomato Trx h was shown to have a characteristic ``shoulder`` at ~290 nm, while the NTR protein had the 3 typical peaks unique to flavoenzymes. The activities of both proteins were demonstrated by following insulin reduction, as well as DTNB reduction. Moreover, both NADPH and NADH could serve as substrates in the NTR reduction system, but the catalytic efficiency of NTR with NADPH was 2500-fold higher than with NADH. Additionally, our results reveal that the tomato Trx system might be involved in oxidative stress, but not in cold damage. [BMB reports 2011; 44(10): 692-697]
Dai, Chang-Bo,Wang, Myeong-Hyeon The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.2
The cDNA clone, SlPMSR, which codes for a putative methionine sulfoxide reductase (PMSR), was isolated from the tomato plant (Solanum lycopersicum L.). The PMSR protein, which could reduce methionine sulfoxide back to methionine, shared a high degree of identity with those proteins isolated from Arabidopsis thaliana (76%), Gossypium barbadense (75%), and Lactuca sativa (70%). The SlPMSR gene exists as at least one copy in the tomato plant, and its transcription was expressed strongly in the leaves and red mature fruits. The SlPMSR gene could be induced constantly by abscisic acid (ABA). Relatively high levels of SlPMSR transcripts were detected 6 h after salicylic acid (SA) treatment, and the transcript levels decreased rapidly until 12 h. The expression of the SlPMSR gene increased at 3 h after $H_2O_2$ treatment, and maintained a constant expression until 24 h. The transcripts of SlPMSR were unchanged after exposure to cold and were induced by mannitol and NaCl, and were increased slightly after treatment. These results indicate that SlPMSR may perform an important function in protecting the plant against abiotic and oxidative stresses.
( Chang Bo Dai ),( Naresh Kumar Singh ),( Myung Ho Park ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.12
Methionine sulfoxide reductase A (MSRA) is a ubiquitous enzyme that has been demonstrated to reduce the S enantiomer of methionine sulfoxide (MetSO) to methionine (Met) and can protect cells against oxidative damage. In this study, we isolated a novel MSRA (SlMSRA2) from Micro-Tom (Solanum lycopersicum L. cv. Micro-Tom) and characterized it by subcloning the coding sequence into a pET expression system. Purified recombinant protein was assayed by HPLC after expression and refolding. This analysis revealed the absolute specificity for methionine-S-sulfoxide and the enzyme was able to convert both free and protein-bound MetSO to Met in the presence of DTT. In addition, the optimal pH, appropriate temperature, and Km and Kcat values for MSRA2 were observed as 8.5, 25oC, 352 ± 25 μM, and 0.066 ± 0.009 S-1, respectively. Disk inhibition and growth rate assays indicated that SlMSRA2 may play an essential function in protecting E. coli against oxidative damage. [BMB reports 2011; 44(12): 805-810]
( Chang Bo Dai ),( Myeong Hyeon Wang ) 한국응용생명화학회 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.2
The cDNA clone, SlPMSR, which codes for a putative methionine sulfoxide reductase (PMSR), was isolated from the tomato plant (Solanum lycopersicum L.). The PMSR protein, which could reduce methionine sulfoxide back to methionine, shared a high degree of identity with those proteins isolated from Arabidopsis thaliana (76%), Gossypium barbadense (75%), and Lactuca sativa (70%). The SlPMSR gene exists as at least one copy in the tomato plant, and its transcription was expressed strongly in the leaves and red mature fruits. The SlPMSR gene could be induced constantly by abscisic acid (ABA). Relatively high levels of SlPMSR transcripts were detected 6 h after salicylic acid (SA) treatment, and the transcript levels decreased rapidly until 12 h. The expression of the SlPMSR gene increased at 3 h after H2O2 treatment, and maintained a constant expression until 24 h. The transcripts of SlPMSR were unchanged after exposure to cold and were induced by mannitol and NaCl, and were increased slightly after treatment. These results indicate that SlPMSR may perform an important function in protecting the plant against abiotic and oxidative stresses.
Dai, Zhengfei,Lee, Chul-Soon,Kim, Bo-Young,Kwak, Chang-Hoon,Yoon, Ji-Wook,Jeong, Hyun-Mook,Lee, Jong-Heun American Chemical Society 2014 ACS APPLIED MATERIALS & INTERFACES Vol.6 No.18
<P>The use of composite materials and polynary compounds is a promising strategy to promote conductometric sensor performances. The perovskite oxides provide various compositional combinations between different oxides for tuning gas-sensing reaction and endowing rich oxygen deficiencies for preferable gas adsorption. Herein, a sacrificial colloidal template approach is exploited to fabricate crystalline ternary LaFeO<SUB>3</SUB> perovskite porous thin films, by transferring a La<SUP>3+</SUP>–Fe<SUP>3+</SUP> hybrid solution-dipped template onto a substrate and sequent heat treatment. The honeycomb-like LaFeO<SUB>3</SUB> film consisted of monolayer periodic pore (size: ∼ 500 nm) array can be successfully in situ synthesized in a homogeneous layout with a single phase of perovskite. This periodic porous LaFeO<SUB>3</SUB> film with p-type semiconductivity exhibits a high gas response, fast response (∼4 s), trace detection capacity (50 ppb), and favorable ethanol selectivity from similar acetone. It exhibits enhanced sensing performances compared to those of a binary n-type Fe<SUB>2</SUB>O<SUB>3</SUB> film and a nontemplated dense LaFeO<SUB>3</SUB> film. In addition, a five-axe spiderweb diagram is introduced to make a feasible evaluation of the optimal practical work condition, comprehensively regarding the response/recovery rate, gas response, selectivity and operating temperature. The enhanced ethanol sensing mechanism of honeycomb-like LaFeO<SUB>3</SUB> periodic porous film is also addressed. This novel and facile route to fabricate well-ordered porous LaFeO<SUB>3</SUB> thin film can also be applied to many fields to obtain special performances, such as solar cells, ion conductors, gas separation, piezoelectricity, and self-powered sensing device system.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2014/aamick.2014.6.issue-18/am504386q/production/images/medium/am-2014-04386q_0008.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am504386q'>ACS Electronic Supporting Info</A></P>
Sun, Chang-Qing,Chang, Yu-Bo,Cui, Ling-Ling,Chen, Jia-Jun,Sun, Nan,Zhang, Wei-Jie,Jia, Xiao-Can,Tian, Yuan,Dai, Li-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
Gastric cancer is the second most common cause of cancer-related deaths in the world. Although certain dietary factors and lifestyles have been suggested to be associated with gastric carcinogenesis, there have been few investigations focusing on rural areas. A case-control study was therefore carried out to investigate the risk factors of gastric cardia cancer (GCC) in rural areas of Linzhou. A total of 470 newly diagnosed cases of GCC and 470 healthy controls were included. Face-to-face interviews were conducted, using a uniform questionnaire containing questions on demographics, per capita income, living habits, dietary habits and family history of tumors. The relationship between putative risk factors and GCC was assessed by odds ratios (OR) and their 95% confidence intervals (95%CI) derived from conditional logistic regression model by the COXREG command using SPSS 12.00. Multivariate logistic regression analysis was used to evaluate simultaneously the effects of multiple factors and other potential confounding factors. Multivariate logistic analysis showed that smoking (OR=1.939, 95%CI:1.097-3.426), alcohol drinking (OR=2.360, 95%CI: 1.292-4.311), hot food consumption (OR=2.034, 95%CI: 1.507-2.745), fast eating (OR=1.616, 95%CI: 1.171-2.230), mouldy food (OR=4.564, 95%CI: 2.682-7.767), leftover food (OR=1.881. 95%CI: 1.324-2.671), and family history of tumor (OR=2.831, 95%CI: 1.588-5.050) were risk factors for GCC. High per capita income (OR=0.709, 95%CI: 0.533-0.942), high education level (OR=0.354, 95%CI: 0.163-0.765), consumption of fresh fruits (OR=0.186, 95%CI: 0.111-0.311) and vegetables (OR=0.243, 95%CI: 0.142-0.415), and high BMI (OR=0.367, 95%CI: 0.242-0.557) were protective factors for GCC. Our data indicate that unhealthy lifestyle and dietary habits might be important contributors to GCC in this population.
Genome-wide identification of the ARRs gene family in tobacco (Nicotiana tabacum)
Jing Lv,Chang-bo Dai,Wei-feng Wang,Yu-he Sun 한국유전학회 2021 Genes & Genomics Vol.43 No.6
Background The growth of axillary buds determines the shoot branching and morphology of plants, and its initiation and development are regulated by a series of hormonal signals, such as cytokinin. Arabidopsis response regulators (ARRs) can regulate the growth and development, disease resistance and stress resistance of plants by participating in cytokinin signaling. Objective To explore the distribution and expression pattern of ARR members in tobacco. Methods The identifcation, isoelectric points, molecular weights, protein subcellular localization prediction, multiple sequence alignment, phylogenetic analysis, protein motifs and structures, chromosome distributions of deduced ARR proteins were conducted. The gene expression profling of various tissues in response to topping, low temperature and drought were analyzed by RNA-seq and qRT-PCR. Results 59 ARR genes from cultivated tobacco (Nicotiana tabacum) were identifed, namely NtARRs, including 21 type A NtARRs and 38 type B NtARRs. The 59 NtARRs were expressed mainly in all organs except the fruits. Some representative NtARRs may participate in axillary bud initiation and development, as well as in stress resistance through cytokinin signal transduction. Conclusion Understanding the roles of NtARRs in the molecular mechanisms responsible for axillary bud growth and stress tolerance could aid in targeted breeding in crops.